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Citations for PerkinElmer :
1 - 50 of 88 citations for IL 13 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
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bioRxiv - Microbiology 2023Quote: Apical wash fractions collected pre- and post-infection were used to detect Lactate dehydrogenase activity and IL-8 concentrations using human LDH and IL-8 Alphalisa Kits (Perkin Elmer). ELISA Kits for the detection of human TNFα ...
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bioRxiv - Cell Biology 2022Quote: ... Confocal sections of 13 (Perkin Elmer) or 17 (Andor ...
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bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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bioRxiv - Cancer Biology 2023Quote: ... Europium labelled anti-His (Perkin Elmer # AD0110) and XL665 labelled streptavidin (CIS Bio 610SAXAC ...
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bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells were seeded in 384-well PhenoPlates (PerkinElmer #6057302) manually coated with collagen I in T-REx base media supplemented with doxycycline (1 ug/mL) ...
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bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells were seeded in 96-well PhenoPlates (PerkinElmer #6055302) manually coated with collagen I in T-REx base media supplemented with doxycycline (1 ug/mL) ...
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bioRxiv - Bioengineering 2022Quote: ... HEK293-ACE2 cells were seeded in 384-well white plates (PerkinElmer, USA), allowed to attach overnight followed by addition of pseudoviral particles in the presence or absence of varying concentrations of GRFT ...
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bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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bioRxiv - Microbiology 2019Quote: ... Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer) were added at final concentration 10 mg/mL to each well and incubated on RT for 3 h ...
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bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
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bioRxiv - Biophysics 2019Quote: ... and detected on a Si APD detector (SPCM-ARQH-13, Perkin Elmer). Microscope control and image acquisition arose from a custom LabView program running on an FPGA (PCIe-7842R ...
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bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
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bioRxiv - Molecular Biology 2020Quote: ... with the DNA 1K / 12K / Hi Sensitivity Assay LabChip (Perkin Elmer) and the LabChip DNA High Sensitivity Reagent Kit ...
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bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells stably transfected with LRRK2 plasmids were seeded in 96-well PhenoPlates (PerkinElmer #6055302) manually coated with collagen I in T-REx base media ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ...
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bioRxiv - Biophysics 2021Quote: ... HEK293 TauRD-P301S-CFP/YFP expressing biosensor cells were plated in poly-L-lysine coated 96-well plates (PerkinElmer) at a density of 20000 cells/well and subsequently transfected with pre-incubated mixtures of tau seeds/CAP1 ...
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bioRxiv - Bioengineering 2021Quote: ... we used the TTL signal from an avalanche photo diode (APD, SPCM-AQRH-13-FC, Perkin Elmer). We illuminated the APD with natural light ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg/mL acceptor (nickel chelate) and donor (anti-his europium; both Perkin Elmer). All components were diluted to working concentrations in alphaLISA buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Bioengineering 2021Quote: ... The RVA (RVA 4800; Perten and PerkinElmer Instruments, Springfield, IL) was performed for 23 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... supernatants were collected and IL-6 assayed with AlphaLISA (PerkinElmer) using the manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
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bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
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bioRxiv - Biophysics 2021Quote: ... using the Hamamatsu ImagEM C9100-13/14 EMCCD Camera integrated with an Olympus IX83 microscope by Perkin Elmer. Samples were imaged using a 100X 1.63N.A ...
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bioRxiv - Cell Biology 2019Quote: ... We also used an Olympus IX81 inverted microscope equipped with a Hamamatsu C9100-13 EMCCD camera and controlled with Volocity 6.3.0 (PerkinElmer). For super-resolution imaging ...
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bioRxiv - Cell Biology 2020Quote: Two days after transfection the Hek293 cells were washed with PBS and seeded into a 96 well black-white iso plate (Perkin Elmer) in pbs containing 5 mM glucose and supplemented with 5 µM of Nanoglo (Promega #N1120 ...
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bioRxiv - Cancer Biology 2019Quote: ... plates were imaged using an IVIS 100 camera (PerkinElmer, Downers Grove, IL) using the following settings ...
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bioRxiv - Neuroscience 2019Quote: ... the animals were anesthetized with 5 mM Levamisole and imaged using 488 nm laser excitation with a Yokogawa CSU-X1-A3 spinning disc confocal head and a Hamamatsu C9100-13 EMCCD Camera controlled by Volocity collectively supplied by PerkinElmer. Imaging was done under 100X 1.4 NA objective using 300 ms exposure at 3 frames/second for a total of 3 minutes for juIs338 and 600 ms exposure at 1.6 frames/second for a total of 3 minutes for wyIs291 ...
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bioRxiv - Biophysics 2021Quote: ... using a 100X/1.4 NA oil objective on the Olympus IX83 microscope integrated with the Yokogawa CSU-X1-A3 spinning disc and Hamamatsu ImagEM C9100-13/14 EMCCD Camera (by Perkin Elmer). The analysis for vesicle accumulation following axotomy was conducted as follows-
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bioRxiv - Cell Biology 2020Quote: ... mounted on a TMC isolation platform (Technical Manufacturing Corporation) with a Yokogawa CSU 10 spinning disc head and Hamamatsu C9100-13 EMCCD camera (Perkin Elmer) with diode lasers of 491 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... The re-collimated beam is divided by means of a 50/50 non-polarising beam splitter cube and is detected by two single-photon avalanche diode detectors (SPCM-AQR-13, Perkin Elmer). Time-traces of fluorescence intensity from the two detectors were cross-correlate to avoid after-pulsing artefacts ...
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bioRxiv - Cell Biology 2019Quote: ... mounted on a TMC isolation platform (Technical Manufacturing Corporation) with a Yokogawa CSU 10 spinning disc head and Hamamatsu C9100-13 ImagEM EMCCD camera (Perkin Elmer) with diode lasers of 491 nm ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons for soma/dendrite tracing were imaged with an Olympus 1X81 spinning disk confocal microscope using a Hamamatsu C9100-13 EM-CDD camera and Volocity software (Perkin Elmer). Synapses were imaged with a Leica SP8 Lightning Confocal/Light Sheet point scanning confocal microscope and LAS X software.
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bioRxiv - Neuroscience 2023Quote: ... The reflected light passed through a band-pass filter (FF01-623/24-25, Semrock) and was focused on an avalanche photodiode (APD, SPCM-AQR-13, PerkinElmer Optoelectronics). The transmitted light passed through a band-pass filter (FF01-676/37-25 ...
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bioRxiv - Neuroscience 2023Quote: ... The transmitted light passed through a band-pass filter (FF01-676/37-25, Semrock) and was focused on a second avalanche photodiode detector (APD, SPCM-AQR-13, PerkinElmer Optoelectronics).
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Microbiology 2021Quote: ... Luminescence was recorded using a Microplate Luminometer or IVIS imaging system (Perkin Elmer, IL)
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bioRxiv - Cell Biology 2021Quote: AlphaLISA™ Immunoassay was used to assess the IL-6 cytokine release (PerkinElmer, #AL223) in the media in 2D and 3D fibroblast cell cultures ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Microbiology 2020Quote: ... as previously described.30 Percentage of the infected cells in each well was automatically obtained from 13 fields per well using Harmony software (PerkinElmer, Waltham, MA, USA). Data was normalized to the infected control ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... In order to quantify IL-6 and TNF-α LANCE® Ultra Detection Kits (PerkinElmer, Inc ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
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bioRxiv - Cell Biology 2021Quote: ... using a Nikon Apo TIRF 100x 1.49 NA oil-immersion objective and a Hamamatsu ImagEM C9100-13 EMCCD camera operated by Volocity software (PerkinElmer, Waltham, MA, US, version 6.4.0). TIRF MT nucleation experiments were performed at 35 °C using a dual-view Leica TIRF microscope ...
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bioRxiv - Biophysics 2022Quote: ... The detectors used are single-photon avalanche photodiodes (SPAD; Donor Channel: 100 μm, MPD PD1CTC, Acceptor Channel: 170 μm, Perkin Elmer SPCM-AQRH 13). The data is correlated by the HydraHarp400 time correlated single photon counting (TCSPC ...
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bioRxiv - Immunology 2023Quote: ... using anti-human IFN-γ capture antibody (Pharmingen, 551221), biotinylated anti-human IFN-γ detection antibody (Pharmingen, 554550) and DELFIA Europium-labelled streptavidin (PerkinElmer), for fluorescent detection and quantification.