Labshake search
Citations for PerkinElmer :
1 - 50 of 116 citations for IL 12 Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells were seeded in 384-well PhenoPlates (PerkinElmer #6057302) manually coated with collagen I in T-REx base media supplemented with doxycycline (1 ug/mL) ...
-
bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells were seeded in 96-well PhenoPlates (PerkinElmer #6055302) manually coated with collagen I in T-REx base media supplemented with doxycycline (1 ug/mL) ...
-
bioRxiv - Bioengineering 2022Quote: ... HEK293-ACE2 cells were seeded in 384-well white plates (PerkinElmer, USA), allowed to attach overnight followed by addition of pseudoviral particles in the presence or absence of varying concentrations of GRFT ...
-
bioRxiv - Genetics 2019Quote: ... and fluorescein-12-dUTP (Perkin Elmer), respectively ...
-
bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells stably transfected with LRRK2 plasmids were seeded in 96-well PhenoPlates (PerkinElmer #6055302) manually coated with collagen I in T-REx base media ...
-
bioRxiv - Microbiology 2023Quote: Apical wash fractions collected pre- and post-infection were used to detect Lactate dehydrogenase activity and IL-8 concentrations using human LDH and IL-8 Alphalisa Kits (Perkin Elmer). ELISA Kits for the detection of human TNFα ...
-
bioRxiv - Biochemistry 2022Quote: ... 0.1 mM FAM-12-dCTP (Perkin Elmer) and 0.5 U/µL DNA Polymerase I Large (Klenow ...
-
bioRxiv - Biophysics 2021Quote: ... HEK293 TauRD-P301S-CFP/YFP expressing biosensor cells were plated in poly-L-lysine coated 96-well plates (PerkinElmer) at a density of 20000 cells/well and subsequently transfected with pre-incubated mixtures of tau seeds/CAP1 ...
-
bioRxiv - Bioengineering 2021Quote: ... The RVA (RVA 4800; Perten and PerkinElmer Instruments, Springfield, IL) was performed for 23 min ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... supernatants were collected and IL-6 assayed with AlphaLISA (PerkinElmer) using the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2019Quote: ... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
-
bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
-
bioRxiv - Cell Biology 2020Quote: Two days after transfection the Hek293 cells were washed with PBS and seeded into a 96 well black-white iso plate (Perkin Elmer) in pbs containing 5 mM glucose and supplemented with 5 µM of Nanoglo (Promega #N1120 ...
-
bioRxiv - Synthetic Biology 2019Quote: ... and fluorescein-12-ddUTP (Perkin Elmer, San Jose, CA) by incubating 0.1mg of beads with 5μM nucleotide and 20U of enzyme in 20mM Tris-HCl pH7.5 ...
-
bioRxiv - Cell Biology 2023Quote: ... and the NEXTFLEX-HT Barcodes (PerkinElmer, NOVA-5188-12). The DNA in the libraries were visualized using the FlashGelTM DNA Cassette (Lonza ...
-
bioRxiv - Cancer Biology 2019Quote: ... plates were imaged using an IVIS 100 camera (PerkinElmer, Downers Grove, IL) using the following settings ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
-
bioRxiv - Microbiology 2021Quote: ... Luminescence was recorded using a Microplate Luminometer or IVIS imaging system (Perkin Elmer, IL)
-
bioRxiv - Cell Biology 2021Quote: AlphaLISA™ Immunoassay was used to assess the IL-6 cytokine release (PerkinElmer, #AL223) in the media in 2D and 3D fibroblast cell cultures ...
-
bioRxiv - Biophysics 2020Quote: ... and 12 μL of either EasyTag EXPRESS35S Protein Labeling Mix (Perkin Elmer) or L-[35S]-Met (Perkin Elmer) ...
-
bioRxiv - Microbiology 2021Quote: ... and 12 dpi by IVIS® Spectrum In Vivo Imaging System (PerkinElmer) 10 min after i.n delivery of 200 μg D-luciferin (LUCK ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... In order to quantify IL-6 and TNF-α LANCE® Ultra Detection Kits (PerkinElmer, Inc ...
-
bioRxiv - Biochemistry 2024Quote: ... 12 µL of ATP premix (0.1µL of ATPγ32P (6,000 Ci/mmol; Perkin Elmer); ATPase Buffer 1X ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 12 and 24 h using an Ensight Multimode Plate Reader (PerkinElmer, Waltham, MA, USA).
-
bioRxiv - Genetics 2023Quote: ... Cells were then homogenized using an Omni Bead Rupter 12 (Perkin Elmer; Kennesaw, GA). Protein lysates were then incubated on ice for thirty minutes ...
-
bioRxiv - Microbiology 2023Quote: ... 535 nm)) was measured after 12 hours using a Victor plate reader (Perkin Elmer). Eighty-nine compounds showing activity were re-tested with the lasB-gfp (31 ...
-
bioRxiv - Cancer Biology 2024Quote: 12 RNA libraries were prepared with the NextFlex Rapid Directional mRNA-Seq kit (Perkin Elmer) with polyA+ mRNA enrichment ...
-
bioRxiv - Immunology 2023Quote: ... uptake inhibition and degradation were determined in M[0] and M[IL-4] using 3H-choline chloride (Perkin Elmer) as previously described [4].
-
bioRxiv - Microbiology 2022Quote: ... The AlphaLISA assay development was performed in 96-well 12 area Alphaplate (reference 6052340, PerkinElmer, Waltham, MA) with a final reaction volume of 40 μL ...
-
bioRxiv - Neuroscience 2021Quote: ... Three-plex detection of IL-4Ra on glutaminergic and GABAergic neurons was achieved using Opal 520 (1:1000, Perkin Elmer), Opal 570 (1:1500 ...
-
bioRxiv - Immunology 2022Quote: ... and interleukin 10 (IL-10) were quantified in the supernatant from the PBMCs using an AlphaLISA assay (PerkinElmer, Waltham, MA). AlphaPlates containing 2 µL of supernatant were thawed at 25°C for 15 minutes ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were seeded in either 12-well plates (0.1× 106 cells) or 96-well CellCarrier Ultra plates (8,000 cells, PerkinElmer) before transfecting with 40 nM ONTARGETplus Non-targeting oligo siRNA (NT1 siRNA ...
-
bioRxiv - Microbiology 2022Quote: ... the cell monolayers were scratched with a 200 μl pipette tip and then imaged every 20 min for 12 h with Harmony High-Content Imaging and Analysis Software version 4.9 (PerkinElmer) and an Opera Phenix High Content Screening System (PerkinElmer ...
-
bioRxiv - Molecular Biology 2021Quote: ... CHO-K1 cells were cultured in F-12 medium with 10% FBS and seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer). The wild-type (WT ...
-
bioRxiv - Developmental Biology 2020Quote: ... mouse dissociated commissural neurons and mouse dorsal spinal cord explants were imaged using a spinning disk confocal system (Perkin Elmer) built on a Nikon Ti-U inverted microscope using a Nikon 40X objective (for nerve cords and neurons ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were imaged for 2 hr prior to and 12 hr following TNFα addition using the Opera Phenix Plus High-Content Screening System (PerkinElmer), with two biological replicates (n = 2) ...
-
bioRxiv - Plant Biology 2024Quote: ... Proteins were resolved by 12% SDS-PAGE and dried gels were exposed to a Cyclone Plus Storage Phosphor Screen (PerkinElmer). The screen was scanned using a Typhoon TRIO Variable Mode Imager (GE Healthcare).
-
bioRxiv - Molecular Biology 2023Quote: ... and 10 μL of 12 nM biotinylated nucleosome (or its mutants) were mixed and incubated in OptiPlate-384 microplate (PerkinElmer) for 60 mins ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5 µl of anti-Mouse Acceptor beads (PerkinElmer, diluted 1:500 with 1x detection buffer ...
-
bioRxiv - Bioengineering 2022Quote: ... OsteoSense 680EX (80 nmol/kg/mouse, PerkinElmer, NEV10020EX). After 48 hours ...
-
bioRxiv - Cancer Biology 2019Quote: ... 5 µl of anti-Mouse Acceptor beads (PerkinElmer, diluted 1:500 with 1x detection buffer ...
-
bioRxiv - Immunology 2021Quote: ... were plated in 3 ml of complete medium supplemented with IL-2 (20 IU/ml) and incubated with 1 μl of 3H-labeled ROL (PerkinElmer, Boston, MA, USA) for 4 h ...
-
bioRxiv - Biochemistry 2023Quote: The expression of IL-6 and TNF-α in culture supernatants was detected using the corresponding AlphaLISA Immunoassay Kit (PerkinElmer, AL223C and AL208C) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2021Quote: ... and then living mouse and mouse organs were imaged and detected using the small animal in vivo imaging system (PerkinElmer, USA). The mouse organs were fixed with formalin ...
-
bioRxiv - Cancer Biology 2020Quote: ... the extent of lung metastasis was measured every 3 days for 12 days using non-invasive imaging with IVIS Lumina III (Perkin Elmer). In brief ...
-
bioRxiv - Neuroscience 2019Quote: ... The signal of CB1 hybridization was revealed by a tyramide signal amplification (TSA) reaction using FITC-labeled tyramide (1:80 for 12 min, Perkin Elmer). After several washes the free-floating slices were incubated overnight with Streptavidin-Texas Red (1 ...
-
bioRxiv - Physiology 2021Quote: ... a longer time was required to achieve steady state) a single bolus of 12 μCi of [1-14C]-2-deoxy-D-glucose (Perkin Elmer) in 96 μl of saline was administered ...
-
bioRxiv - Cell Biology 2022Quote: ... 12 pictures of at least 3 biological replicates were quantified using the Harmony high-content imaging and analysis software (Perkin Elmer).
-
bioRxiv - Immunology 2023Quote: ... B cells were lysed in water by osmotic pressure and were transferred from 96 well plates to glass fiber filtermat (8×12 Filtermat A, GF/C, 100/pk, #1450-421, Perkin Elmer). The filters were rinsed with water and sealed in plastic bags with a scintillation cocktail (OptiScint® LLT ...