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Citations for PerkinElmer :
1 - 50 of 355 citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... was captured on anti-mouse IgG (Fc specific) acceptor beads (Perkin Elmer, Cat nr. AL105C). Serial dilutions of antibodies and VHH-Fc (final concentration ranging between 100nM - 0,001 nM ...
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bioRxiv - Cell Biology 2023Quote: Pulse-chase experiments using 9.8 Mbq per dish of EASY-TAG EXPRESS Protein labeling mix [35S] (PerkinElmer) and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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bioRxiv - Bioengineering 2024Quote: ... Vivo-Tag 680 XL (PerkinElmer #NEV11119) was used as a fluorescent dye to track idLFA1 and control murine serum albumin (MSA ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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bioRxiv - Neuroscience 2021Quote: ... oil objective on a NikonTi-E stand (PerkinElmer) with a Hamamatsu C9100–50 camera ...
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bioRxiv - Cell Biology 2019Quote: ... Easy-Tag [35S]-Cys/Met was purchased from PerkinElmer Life Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
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bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
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bioRxiv - Biophysics 2021Quote: ... Chemical structure of E- 4031 was drawn using ChemDraw (PerkinElmer) and converted to a 3D structure using OpenBabel (O’Boyle et al. ...
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bioRxiv - Cell Biology 2021Quote: ... and 100μl Easy Tag Express 35S Methionine/Cysteine (Perkin Elmer NEG 772002MC) at either 0 or 24h after transformation with a 6xMyc-Pex14 (pAMW-Pex14 ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Bioengineering 2022Quote: ... Two avalanche photodiodes detect the fluorescent photons (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
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bioRxiv - Cell Biology 2021Quote: ... Imaging was done with temperature-controlled Nikon Ti-E Eclipse inverted/UltraVIEW VoX (Perkin Elmer) spinning disc confocal setup ...
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bioRxiv - Molecular Biology 2024Quote: ... approximately 1 μg of plasmid DNA and 20 μCi of Easy-tag 35S-methionine (Perkin Elmer) were mixed with a final volume of 50 μl ...
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bioRxiv - Cell Biology 2022Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
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bioRxiv - Biophysics 2020Quote: ... The fluorescence was directed to a fiber coupled APD (SPCM-AQR-14-FC, Perkin Elmer), which was connected to a time-correlated single photon counting module (SPC830 ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
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bioRxiv - Cell Biology 2023Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
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bioRxiv - Genomics 2019Quote: ... Anti-rabbit or anti-mouse HRP conjugated antibodies were used to detect protein by chemiluminescence using ECL (Perkin Elmer, Western Lightning Plus ECL). Images were visualized by GeneSnap image analysis software (SynGene ...
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bioRxiv - Neuroscience 2022Quote: ... The fluorescence signal was confocally detected by an avalanche photodiode (APD; SPCM-AQRH-14-FC; PerkinElmer). The spatial resolution of the setup was around 175 nm (in x-y ...
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bioRxiv - Neuroscience 2019Quote: ... The fluorescence signal was confocally detected by an avalanche photodiode (APD; SPCM-AQRH-14-FC; PerkinElmer). The spatial resolution of the setup was around 175 nm (in x-y ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Bioengineering 2021Quote: ... we used the TTL signal from an avalanche photo diode (APD, SPCM-AQRH-13-FC, Perkin Elmer). We illuminated the APD with natural light ...
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bioRxiv - Cell Biology 2021Quote: All widefield images were captured on an inverted Nikon TE2000-E widefield microscope with Volocity Acquisition software (PerkinElmer). These objective lenses were used ...
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bioRxiv - Biophysics 2023Quote: ... CFTR variants were pulse-labeled with 0.1-0.2 mCi/ml 35S-methionine and 35S-cysteine (Easy Tag Express, Perkin Elmer) in Cys- and Met-free DMEM (Gibco™ 21013024 ...
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bioRxiv - Immunology 2021Quote: ... of the abdominal region was measured (exposure=1 sec., F/stop= 1.2, FOV=E, binning=medium, emissions filter=open) using Living Image software (PerkinElmer). Survival of the mice was recorded until 20 days after infection.
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bioRxiv - Cell Biology 2021Quote: ... Worms were imaged on a 2% agarose pad on a Nikon Ti-E Eclipse inverted/UltraVIEW VoX (Perkin Elmer) spinning disc confocal setup ...
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bioRxiv - Molecular Biology 2023Quote: ... Imaging was performed on a Nikon ECLIPSE Ti-E-inverted microscope equipped with a spinning disk device (Perkin Elmer Ultraview ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Cell Biology 2021Quote: ... on the Nikon TE2000-E microscope equipped with an ORCA-ER CCD camera (Hammamatsu Photonics) operated by Volocity Acquisition module software (Improvision, PerkinElmer). Collagen degradation was quantified using Fiji by measuring the integrated density of the collagen layer ...
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bioRxiv - Molecular Biology 2020Quote: ... Imaging was done on a Nikon ECLIPSE Ti-E-inverted microscope equipped with a spinning disk device (Perkin Elmer Ultraview), and images were processed using Volocity software (version 6.2.1 ...
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bioRxiv - Neuroscience 2021Quote: ... we used a spinning disc confocal microscope (Nikon Ti-E Eclipse inverted scope equipped with 488 and 561 laser lines, a Prior NZ250 Piezo stage, a PerkinElmer UltraVIEW VoX ...
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bioRxiv - Cancer Biology 2021Quote: Quantitation of each individual tumor was measured from H&E sections using morphometric analysis in Panoramic viewer software (Perkin Elmer), which calculates the size of each identified tumor by area in squared microns ...
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bioRxiv - Cell Biology 2021Quote: ... worms were immobilized with imidazole and imaged on a temperature-controlled Nikon Ti-E Eclipse inverted/UltraVIEW VoX (Perkin Elmer) spinning disc confocal setup ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Neuroscience 2022Quote: ... The medium was removed and cells were incubated with 500 nM of SNAP-Red in Tag-Lite Buffer (Perkin Elmer Cisbio) for 1 hr at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and imaged onto the active area of two fibre-coupled avalanche photodiode detectors (APDs; Perkin Elmer, SPCM-AQRH-15-FC) using a cubic non-polarizing beam-splitter (Linos ...
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bioRxiv - Cell Biology 2023Quote: ... EXPRE35S35S Protein Labelling Mix (PerkinElmer); 150 μCi/10 plate] for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: Laser-ablation and fluorescent live imaging of live myocultures were performed on a Nikon Eclipse TE 2000-E inverted spinning disc microscope (Ultraview ERS, Perkin Elmer) and a near-infrared (NIR ...
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bioRxiv - Cell Biology 2022Quote: ... Tumor size of each individual tumor was measured from H&E stained sections using morphometric analysis in Panoramic viewer software (Perkin Elmer). Tumor burden ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
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bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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bioRxiv - Biophysics 2020Quote: ... a fast and mild filtration step is performed and the tag-PDZ concentrations were measured by capillary electrophoresis instrument (LabChip GXII, PerkinElmer, Massachusets, USA). A detailed protocol of how to run the holdup assay in an automatic way using liquid handling robots can be found in [29] ...
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bioRxiv - Neuroscience 2023Quote: ... brains were incubated during 2 weeks at 37°C in presence of an AT8 antibody that specifically binds hyperphosphorylated tau (pSer202/Thr205/PSer208, produced at Janssen Pharmaceutica) and is directly labelled with a near-infrared fluorescent tag (PerkinElmer VivoTag 680XL). Brain samples were then dehydrated ...
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bioRxiv - Biophysics 2019Quote: ... and a 525/50 nm band-pass filter (F37-516, AHF, Germany) and directed to a fibre-coupled avalanche photo-diode (SPCM-AQR-14-FC, Perkin Elmer) connected to a time-correlated single photon counting (TCSPC ...
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bioRxiv - Bioengineering 2020Quote: MTs were transferred with a tip quickly coated in fetal calf serum (FCS) into a CellCarrier-384 Ultra Microplates (6057300, Perkin Elmer). They were then fixed in 4% PFA (P6148 ...
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bioRxiv - Microbiology 2023Quote: ... and a 525/50 nm band-pass filter (F37-516, AHF, Germany) and directed to a fibre-coupled avalanche photo-diode (SPCM-AQR-14-FC, Perkin Elmer) connected to a time-correlated single photon counting (TCSPC ...
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bioRxiv - Biophysics 2024Quote: Single-point FCS data were acquired with an Insight Cell confocal microscope (Evotec Technologies, Hamburg, Germany, now PerkinElmer, Waltham, MA, USA). Excitation was performed using a 488 nm continuous wave solid state diode-pumped laser (Sapphire 488-20/460-10 ...
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bioRxiv - Cell Biology 2021Quote: Cells were imaged on a Nikon Ti-E microscope with a CSU-X1 Yokogawa spinning-disk confocal scan head (PerkinElmer, Wellesley, MA), an Andor iXon+electron-multiplying charge-coupled device camera (Andor) ...