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Citations for PerkinElmer :
1 - 50 of 293 citations for Glial fibrillary acidic protein GFAP Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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bioRxiv - Cancer Biology 2023Quote: ... Europium labelled anti-His (Perkin Elmer # AD0110) and XL665 labelled streptavidin (CIS Bio 610SAXAC ...
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bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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bioRxiv - Microbiology 2019Quote: ... Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer) were added at final concentration 10 mg/mL to each well and incubated on RT for 3 h ...
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bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
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bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
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bioRxiv - Neuroscience 2021Quote: The level of MMP-9 in plasma was measured with an AlphaLISA kit for the human protein (#AL3138, PerkinElmer). AlphaLISA signals were measured using an Ensight Multimode Plate Reader (PerkinElmer).
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bioRxiv - Molecular Biology 2020Quote: ... with the DNA 1K / 12K / Hi Sensitivity Assay LabChip (Perkin Elmer) and the LabChip DNA High Sensitivity Reagent Kit ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg/mL acceptor (nickel chelate) and donor (anti-his europium; both Perkin Elmer). All components were diluted to working concentrations in alphaLISA buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
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bioRxiv - Bioengineering 2023Quote: ... Offline analysis of the β-tub III area and GFAP perimeter were performed using Volocity software (Volocity 3D image and quantification analysis software, PerkinElmer, USA). The images were acquired using identical exposure settings for each set of experiments ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
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bioRxiv - Immunology 2023Quote: ... using anti-human IFN-γ capture antibody (Pharmingen, 551221), biotinylated anti-human IFN-γ detection antibody (Pharmingen, 554550) and DELFIA Europium-labelled streptavidin (PerkinElmer), for fluorescent detection and quantification.
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
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bioRxiv - Cell Biology 2023Quote: ... EXPRE35S35S Protein Labelling Mix (PerkinElmer); 150 μCi/10 plate] for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... Alpha-LISA kits specific for human Aβ1–X (AL288C, PerkinElmer) and Aβ1–42 (AL276C ...
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bioRxiv - Neuroscience 2020Quote: The AlphaLISA human frataxin detection kit (PerkinElmer, catalogue # AL322HV/C/F) was scaled down to a white opaque 384-well plate format (PerkinElmer ...
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bioRxiv - Biochemistry 2019Quote: ... EasyTagTM EXPRESS35S Protein Labeling Mix (PerkinElmer) (1 μl/25-μl reaction ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with anti-myc antibody 9E10 at 4 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: Primary human hepatocytes were seeded in 96-well black CellCarrier plates (PerkinElmer) at a density of 5×103 cells per well ...
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bioRxiv - Immunology 2021Quote: Human fibroblasts cells were seeded on CellCarrier-96well Ultra microplate (Perkin Elmer) and incubated for at least 24 hours in growth media ...
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bioRxiv - Biochemistry 2023Quote: 5x104 human neutrophils were seeded in a black 96-well plate (PerkinElmer) in HyClone HBSS +Ca ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were detected by ECL (Perkin Elmer). Antibodies used are listed in the key Resource Table.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to PVDF membranes (PerkinElmer) and blocked in 5% nonfat milk in PBS ...
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bioRxiv - Cell Biology 2021Quote: The human glioblastoma U87 MG-Red-FLuc (Bioware Brite, PerkinElmer, Waltham, Massachusetts, USA) was the authorized cell line in all experiments ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Multispectral microscopy of the human biopsies was performed using a Vectra 3.0 (PerkinElmer) equipped with 20x and 40x objectives ...
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bioRxiv - Physiology 2020Quote: ... Protein bands were detected using Western Lightning (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Microbiology 2020Quote: ... [35S]-EasyTag™ Express Protein Labeling Mix (PerkinElmer) was added and the cells were incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 µCi/ml and at given time points ...
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bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 μCi/ml and at given time points ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the proteins was assessed by SDS-PAGE and the protein concentration was determined using a spectrometer (PerkinElmer lambda40) with an extinction coefficient of 6990 M−1cm−1.
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer) ...
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bioRxiv - Immunology 2020Quote: ... Bound IgG was detected with Eu-N1 Anti-Human IgG (Perkin Elmer, 1244-330). using DELFIA-TRF system (Perkin Elmer).
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bioRxiv - Genomics 2021Quote: Human primary AMSCs and PHH were plated in 96-well CellCarrier plates (Perkinelmer #6005550). AMSCs were differentiated for 14 days and high content imaging was performed at day 0 ...
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bioRxiv - Cell Biology 2021Quote: Human Fibroblasts Cells were seeded on CellCarrier-384 or 96well Ultra microplate (Perkin Elmer) and incubated for at least 24 h in growth media ...
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bioRxiv - Cell Biology 2019Quote: ... protein bands were visualized with enhanced chemiluminescent reagent (PerkinElmer) and exposed to X-ray films (Carestream ...
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bioRxiv - Immunology 2021Quote: ... proteins were detected using Western Lightning Plus-ECL (PerkinElmer) and the iBright FL1000 Imaging System (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: SPA PVT Protein A beads (500μl/sample, Perkin Elmer) were incubated for 2h with anti-GST antibodies (1:100) ...
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bioRxiv - Biochemistry 2020Quote: ... and EasyTag EXPRESS [35S] Protein Labeling Mix (Perkin Elmer) for 30 min in a tissue culture incubator ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were visualized by enhanced chemiluminescence (PerkinElmer, cat# NEL105001EA). Figures show representative images of 3-5 biological replicates.