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Citations for PerkinElmer :
1 - 50 of 1095 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and underwent antigen retrieval in antigen retrieval buffer (AR9, PerkinElmer) in a 95LC water bath ...
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bioRxiv - Microbiology 2021Quote: ... Gag p24 antigen concentration was measured with the Alliance HIV-1 p24 Antigen ELISA kit (Perkin Elmer).
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bioRxiv - Microbiology 2022Quote: ... by measuring the Gag p24 antigen concentration with the Alliance HIV-1 p24 Antigen ELISA kit (Perkin Elmer).
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bioRxiv - Microbiology 2022Quote: ... Concentration of HIV p24 antigen in each stock was determined by a p24 antigen capture kit (PerkinElmer, Waltham, MA, USA) and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... Antigen retrieval proceeded in AR6 buffer (AR600, PerkinElmer) at 100 °C for 23 min in a pressure cooker ...
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bioRxiv - Microbiology 2019Quote: ... Nluc activity was measured from cell lysates within 10 min of lysis (or substrate addition) by transferring lysate into white OptiPlate 96-well plates (PerkinElmer, Waltham, MA) and measured in a Berthold Centro XS3 LB 960 luminescent plate reader with readings integrated over 0.2 sec.
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bioRxiv - Cancer Biology 2022Quote: ... Slides underwent antigen retrieval in AR9 Buffer (PerkinElmer, AR900250ML) for 1 min (100% Power ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell lysates were combined with 8mL Ultima Gold scintillation cocktail (PerkinElmer 6013329) and CPM was measured using Beckman LS6500 Scintillation counter ...
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bioRxiv - Microbiology 2022Quote: ... HIV-1 replication activity was determined by measuring p24 antigen levels in the culture supernatants using the p24 antigen capture assay (PerkinElmer, Boston, MA, USA) (23).
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were transferred to a 384 OptiPlate (Perkin Elmer, Waltham, MA, USA) and mixed 1:1 with antibody mixture from the IP-ONE-Gq kit ...
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bioRxiv - Biochemistry 2019Quote: ... cell lysate (5 μl/well) was transferred to a 384-well ProxiPlate (PerkinElmer) and added to the donor and acceptor reaction mix (2.5 μl/ well ...
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bioRxiv - Immunology 2022Quote: ... 110 μl of the cell lysate was transferred to a black/white plate (PerkinElmer). Luminescence was measured using a PerkinElmer Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Four μL of cell lysate was transferred to a 384 well ProxiPlate (Perkin Elmer). Immediately prior to use ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cell lysate (5 μL/well) was transferred to a 384-well ProxiPlate (PerkinElmer, Melbourne, Australia) and added to the donor and acceptor reaction mix (2.5 μL/ well ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were incubated with 1 mM GTP/[α-32P]GTP (0.1 μCi/μl) (PerkinElmer) and incubated at 30°C for various time intervals for comparison pYfiN and pYfiN(Ips) ...
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bioRxiv - Immunology 2022Quote: ... Levels of p24 antigen in supernatant were quantified by p24 ELISA (PerkinElmer) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10 μL of the cell lysates were transferred into a white 384 well plate (ProxiPlate, PerkinElmer). 5 μL of the Europium-cAMP tracer (1 to 50 dilution of Eu-tracer stock in cAMP detection buffer ...
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bioRxiv - Physiology 2019Quote: ... Antigen detection was performed using Tyramide signal amplification (Perkin Elmer-TSA-Plus Fluorescein) according to manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL cell lysate was added to 3 mL of Ultima Gold scintillation liquid (Perkin Elmer #6013329). Samples were kept in the dark overnight and 3H-2DG uptake was measured the following day on a Hidex 300 SL scintillation counter ...
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bioRxiv - Physiology 2024Quote: ... 0.5 mL of the cell lysate was counted in a liquid scintillation counter (TRI-CARB 4910TR, PerkinElmer) and protein content of the remaining lysate was measured for normalisation of results (BCA Protein Assay Kit ...
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bioRxiv - Immunology 2022Quote: ... antigens were visualized using a Western Lightning Plus-ECL (Perkin Elmer, 50-904-9323).
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bioRxiv - Microbiology 2021Quote: ... Transduction efficiency was quantified by measuring luciferase activity in cell lysates using Britelite Plus (Perkin Elmer, Richmond, California) and following the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... 14 and 21 for HIV-1 p24 antigen levels by ELISA (Perkin Elmer, Boston, MA).
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bioRxiv - Biochemistry 2021Quote: ... The supernatant and the cell lysate were added into separate scintillation tubes containing Ultima Gold LSC cocktail (Perkin Elmer) and the radioactivity was measured by a TriCarb 3100TR liquid scintillation counter (Perkin Elmer) ...
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bioRxiv - Bioengineering 2021Quote: ... Heat-induced antigen retrieval (HIER) was carried out for all sections in AR6 buffer (AR6001KT, PerkinElmer), pH = 6.00 ...
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were analyzed for HIV-1 p24 antigen by the AlphaLISA HIV p24 kit (PerkinElmer). Virus stocks contained approximate 200 ng/ml of HIV p24 protein.
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bioRxiv - Immunology 2023Quote: Serum samples were serially diluted and transferred onto an antigen-coated and blocked SpectraPlate-96 (PerkinElmer) plate ...
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bioRxiv - Genomics 2019Quote: ... cell lysates were prepared with Passive Lysis Buffer and Luciferase activity was measured on a VICTOR X3 plate reader (PerkinElmer). Luciferase assay values are represented in fold change of Fluc (pGL4.23 plasmid ...
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bioRxiv - Immunology 2021Quote: ... cell lysate was loaded onto a 96-well plate (Grenier-BioOne) and mixed with MICROSCINTTM-20 scintillation fluid (Perkin Elmer). Samples were then incubated at room temperature for 2 hours and incorporated radioactivity analyzed on a 1450 MicroBeta TriLux Microplate Scintillation and Luminescence counter (Perkin Elmer).
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bioRxiv - Microbiology 2023Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer). Viral infectivity was considered positive if the RLU value was at least 5-fold higher than the background RLU value in the parental cell line ...
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bioRxiv - Microbiology 2023Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer). Viral infectivity was considered positive if the RLU value was at least 5-fold higher than the background RLU value in the NP-2 parental cell line ...
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bioRxiv - Microbiology 2023Quote: ... The infectivity in each well was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer). The percentage of inhibition was determined by comparing the infectivity with positive control wells without drug inhibition.
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bioRxiv - Microbiology 2024Quote: ... and the infectivity was determined by measuring the RLUs in the cell lysates using the Britelite plus system (PerkinElmer, USA). Viral infection was considered positive if the RLU value was more than 5-fold higher than the background RLU value in parental NP-2 cell line ...
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bioRxiv - Microbiology 2024Quote: ... The infectivity in each well was determined by measuring the RLU in the cell lysate using the Britelite plus system (PerkinElmer). The percentage of Maraviroc inhibition was determined by comparing the RLU values with the positive control wells without Maraviroc inhibition ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was mixed with Optiphase Hisafe 3 (PerkinElmer), and radioisotope activity was monitored by LSC-8000 β-scintillation (Hitachi) ...
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bioRxiv - Neuroscience 2020Quote: ... The lysate was mixed with Ultima Gold (Perkin Elmer) and the relative expression of Na+-dependent uptake of radiolabeled glutamate was calculated based on the measurement with a microplate scintillation counter (1450 MicroBeta Plus ...
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bioRxiv - Cancer Biology 2021Quote: ... Antigen-antibody complexes were detected with West Pico Chemi luminescent substrate using standard manufactures protocol (Perkin Elmer). Imaging of all western blots was performed using an UVP BioImager 600 system equipped with VisionWorks Life Science software (UVP ...
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bioRxiv - Microbiology 2021Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer, USA). Viral infectivity was considered positive when the RLU value was at least 5-fold higher than the background RLU value in the parental NP-2 cell line ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA® SureFire® Ultra™ assay was run in total volume 20 μL (using only 10 μL cell culture lysate sample) in 384 well plate (PerkinElmer, #6007290) following standard assay two step no-wash protocol ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 16–18 hours at 37°C before Fluc activity in cell lysates was detected using a plate luminometer (EnSpire 2300 Multilabel reader by Perkin Elmer) and the Bright-Glo Luciferase Assays System (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... Cholesterol efflux was determined as % counts per minute (CPM) in the supernatant versus cell lysates with a liquid scintillation analyzer (Tricarb-2810TR, Perkin Elmer).
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bioRxiv - Immunology 2020Quote: ... The integrated signal density corresponding to labeled antigen or antibody in each LN was calculated using LivingImage (PerkinElmer) or ImageJ software and plotted using GraphPad Prism 8 ...
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bioRxiv - Microbiology 2021Quote: Concentration of HIV in each stock was determined by a p24 antigen capture kit (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Systems Biology 2021Quote: ... The His6-MBP-PDZ concentrations of soluble cell lysate fractions were evaluated with a microfluidic capillary electrophoretic system (Caliper LabChip GXII, PerkinElmer, Waltham, Massachusetts) and were adjusted to 4 μM by dilutions ...
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bioRxiv - Biochemistry 2023Quote: ... The other procedures were the same as the Zn transport assay except that the cell lysates were mixed with the Ultima Gold cocktail (PerkinElmer, Cat# L8286) and 55Fe associated with the cells was quantified using a liquid scintillation counter (LS 6500 Multi-Purpose Scintillation Counter).
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bioRxiv - Bioengineering 2021Quote: ... Concentrations of metals in the cell lysates were determined using inductively coupled plasma optical emission spectroscopy (ICPOES; Model Optima 4300D; Perkin Elmer, Norwalk, CT) operated at wavelengths of 238.204 and 259.939 nm for iron ...
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bioRxiv - Biochemistry 2021Quote: ... coli and all constructs were adjusted to approximately 4 μM concentration in cell lysate soluble fractions using a microcapillary electrophoretic system (Caliper; PerkinElmer, Waltham, MA, USA) and frozen in 96-well plates.
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bioRxiv - Immunology 2022Quote: ... Multiplex-immunofluorescence or confocal laser scanning microscopy imaging were performed after antigen retrieval with epitope retrieval buffer (PerkinElmer Inc.). Slides were incubated sequentially for 1h with the following antibodies ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein antigen (2.0µg/mL) was directly adsorbed on half-area 96-well OptiPlate (96W HB, Perkin Elmer) microplates for 2h at 37°C in a humidified incubator.
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bioRxiv - Cell Biology 2020Quote: ... Lysates were placed into a white 96-well plate (Perkin Elmer) to assess Luciferase and Renilla activities using Envision Multilabel plate reader (Perkin Elmer) ...