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Citations for PerkinElmer :
1 - 50 of 193 citations for Autophagy Related 4B Cysteine Peptidase ATG4B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... [35S] methionine/cysteine (EasyTag, PerkinElmer) was added with a final concentration of 400 μCi for pulse labeling assays ...
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bioRxiv - Cell Biology 2021Quote: ... containing 35S methionine/cysteine (Perkin Elmer) in the presence of SP-cells at 30°C for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and L-[35S]cysteine (PerkinElmer Life Sciences), protein molecular weight markers ...
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bioRxiv - Biochemistry 2020Quote: ... and 7 μCi [35S]-Methionine/cysteine (PerkinElmer; NEG072007MC) and labelled for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... incubated at 37°C for 30 min in 500 μl starvation medium (DMEM lacking methionine and cysteine) and another 30 min in 500 μl labeling medium containing 100 μCi/ml [35S]methionine/cysteine (Perkin Elmer). The cells were washed twice with PBS and lysed in 500 μl lysis buffer (PBS with 1% Triton X-100 ...
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bioRxiv - Cell Biology 2020Quote: MCF10A cells transfected with control or ErbB3 siRNA were incubated with growth factor deprived DMEM media lacking methionine and cysteine supplemented with 10 μCi/ml 35S-methionine/cysteine (Perkin Elmer) for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... 0.3 μl [35S]-methionine/cysteine (PerkinElmer,>1000 Ci/mmol), and 75 mM KOAc pH 7.5 at 30 °C for 1 hr ...
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bioRxiv - Molecular Biology 2022Quote: ... After that 200μCi/ml of 35S-methionine/cysteine (PerkinElmer, EasyTag EXPRESS 35S Protein Labeling Mix NEG072014MC ...
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bioRxiv - Microbiology 2019Quote: ... 35S methionine and 35S cysteine were purchased from Perkin Elmer. Alexa Fluor™A647 succinimidyl ester was purchased from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and 30 μCi ml−1 [35S] cysteine (Perkin Elmer #NEG022T) for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... followed by labeling with [35S]methionine/cysteine (PerkinElmer Life Sciences) in the same medium for 15 min ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with either 35S-methionine/S-L-cysteine mix (PerkinElmer; #NEG772002MC) for radiography detection or with untagged Methionine (Promega #L118A ...
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bioRxiv - Molecular Biology 2019Quote: ... 3 mL methione/cysteine-free DMEM containing 100 mCi 35S (Perkin Elmer) was added ...
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bioRxiv - Cell Biology 2022Quote: ... pulsed with 15 uCi/mL 35Smethionine/cysteine (EasyTag EXPRESS 35S, Perkin Elmer) and chased in complete DMEM containing 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... and 100μl Easy Tag Express 35S Methionine/Cysteine (Perkin Elmer NEG 772002MC) at either 0 or 24h after transformation with a 6xMyc-Pex14 (pAMW-Pex14 ...
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bioRxiv - Microbiology 2020Quote: ... and exposed to 15 μCi/ml [35S]-methionine and [35S]-cysteine (Perkin Elmer) from 5-24 hours ...
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bioRxiv - Physiology 2021Quote: ... Subsequently media was supplemented with 250 μCi of [35S]-cysteine/methionine mixture (PerkinElmer, NEG772002MC) and islets were incubated under normal conditions for 30 min ...
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bioRxiv - Physiology 2021Quote: ... Subsequently media was supplemented with 250 μCi of [35S]-cysteine/methionine mixture (PerkinElmer, NEG772002MC) and cells incubated under normal conditions for 30 min ...
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bioRxiv - Immunology 2020Quote: Metabolic labeling was performed using S35-labeled Cysteine/Methionine (Perkin Elmer, Waltham, MA, USA). Cells were seeded to 60%-70% confluence in 6-well plates ...
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bioRxiv - Molecular Biology 2023Quote: ... 200µCi/mL of EasyTag labeling mix (35S-cysteine/35S-methionine, Perkin Elmer CAT NEG772007MC) was added for 30min or 60min ...
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bioRxiv - Cell Biology 2020Quote: Pulse-chase analyses were performed using metabolic radiolabelling with [35S]methionine/cysteine (Perkin Elmer, Waltham, MA, USA) and subsequent immunoprecipitation of specific radiolabeled proteins were conducted as previously described (Alexander et al. ...
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bioRxiv - Microbiology 2022Quote: ... H1299 cells were cultured in the presence of S35-methionine and cysteine (10µCi/ml, NEG772007MC, Perkin Elmer) in methionine/cysteine free MEM at indicated time and duration ...
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bioRxiv - Cell Biology 2022Quote: ... and cells were incubated for 5 min before addition of 200μCi 35S-labeled methionine/cysteine mixture (PerkinElmer) and incubation for 1 hr at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... The medium was then changed to DMEM with cysteine and 0.05 μCi/μl 35S methionine (PerkinElmer, cat. NEG009A). Pulse labeling was done for 30 ...
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bioRxiv - Microbiology 2022Quote: ... BV2 cells plated in 15 cm plates were infected at a MOI of 0.05 for three hours before adding 0.36 mCi of [35S]cysteine-methionine (Perkin Elmer) for 45 h ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 1h in methionine (Met)- and cysteine (Cys)-free DMEM supplemented with 250μCi of 35S-Met/Cys radiolabel (PerkinElmer) per condition at normoxia ...
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bioRxiv - Cell Biology 2021Quote: ... cells were metabolically labelled for 20 min with 55 μ Ci/ml 35S- methionine/cysteine protein labelling mix (PerkinElmer). Cells were washed with PBS and cultured in DMEM (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then supplemented with 7.4 MBq of [35S] methionine and [35S] cysteine (EXPRE35S35S Protein Labeling Mix, [35S]-, PerkinElmer) and incubated for 1 h to specifically label newly synthesized mitochondrial proteins ...
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bioRxiv - Microbiology 2022Quote: ... cells were metabolically labeled for 16 h with 100 μCi/mL [35S] methionine-cysteine ([35S] protein labeling mix; PerkinElmer) in Dulbecco’s modified Eagle’s medium lacking methionine and cysteine and supplemented with 10% of dialyzed fetal bovine serum and 1X GlutaMAX™ (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Reactions contained 10 μL lysate with 250 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine/cysteine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes chasing with 2 μL of 50 mg / mL unlabelled methionine/cysteine ...
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bioRxiv - Biophysics 2023Quote: ... CFTR variants were pulse-labeled with 0.1-0.2 mCi/ml 35S-methionine and 35S-cysteine (Easy Tag Express, Perkin Elmer) in Cys- and Met-free DMEM (Gibco™ 21013024 ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were incubated for 5 minutes at 37°C with 55µCi/well of 35S-L-methionine and 35S-L-cysteine Promix (Perkin Elmer) in a minimal volume of culture medium ...
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bioRxiv - Molecular Biology 2019Quote: Cells were incubated with complete growth media supplementing with EasyTagTM Express Protein Labeling Mix containing both [35S]-L-methionine and [35S]-L-cysteine (PerkinElmer) at 10 μCi/ml for 30 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... newly synthesized proteins in the cells were labeled by treating with 150 μCi/ml of 35S-methionine/cysteine (EXPRE35S35S Protein Labeling Mix, NEG772, PerkinElmer) apically for 45 min (pulse labeling) ...
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bioRxiv - Microbiology 2020Quote: ... 200 μCi (7.4 MBq) [35S]-methionine/L-[35S] cysteine using EasyTag™ EXPRESS35S Protein Labeling Mix (PerkinElmer, Groningen, the Netherlands) was added to the medium ...
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bioRxiv - Cell Biology 2019Quote: ... 3 OD600 units of early log phase cells were labeled with 80 mCi of L-[35S]-methionine/cysteine mix (Perkin Elmer). Immunoprecipitated proteins were separated on SDS-PAGE and exposed to phosphor screens ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in cysteine/methionine-free DMEM for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine/35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in serum-free ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% dialyzed fetal calf serum and then metabolically labelled for 10 min with 55 μCi/ml 35S-methionine/cysteine protein labeling mix (Perkin Elmer). Cells were rinsed immediately with PBS twice and solubilized with 1% Triton X-100 in solubilization buffer (50 mM HEPES-KOH ...
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bioRxiv - Microbiology 2022Quote: ... The production of LepB–SecM and LepB–CutF variants was induced by the simultaneous addition of 0.1 M IPTG and 2 µl of L-[35S] methionine-cysteine (7 mCi/ ml, PerkinElmer Life Sciences) (38) ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were pulsed with 0.1 mCi/ml 35S-L-methionine and 35S-L-cysteine mix (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer) in methionine- and cysteine-free DMEM for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... were harvested in Schneider’s medium on ice and transferred to Schneider’s medium containing 35S-methionine/cysteine (2 µCi/µl; Perkin Elmer Labs Inc., NEG709A) and 30 µM chloroquine (to inhibit autophagy) ...
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bioRxiv - Cell Biology 2022Quote: Confluent 6-well dishes of FRT cells (approximately 1×106/well) were metabolically labeled in DMEM depleted of methionine and cysteine and supplemented with EasyTag 35S Protein Labeling Mix (Perkin Elmer, NEG772007MC), glutamine ...
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bioRxiv - Bioengineering 2022Quote: ... PEG-maleimide was reacted with 1 mol equivalence of calpain substrate peptide and 1 mol equivalence of L-cysteine-functionalized VivoTag S-750 (PerkinElmer, Boston, MA), followed by quenching with an excess of L-cysteine ...
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bioRxiv - Immunology 2021Quote: ... cells were pulse labelled with 50µCi of 35S-methionine and -cysteine for 1 min (EasyTag™ EXPRESS35S Protein Labeling Mix, Perkin Elmer, Waltham MA, USA) and immediately transferred into 10x volume of chase medium containing excess amounts of unlabelled cysteine (500 µg/ml) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... anti-cGMP antibody (PerkinElmer anti-cGMP antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were detected using HRP-linked secondary antibodies together with Western Lightning Plus-ECL (Perkin Elmer). Fiji (ImageJ ...
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bioRxiv - Neuroscience 2020Quote: ... antibodies plus Cy3-TSA (1:200, PerkinElmer). Sections were cover-slipped using Prolong Diamond Antifade Mounting media with DAPI (ThermoFisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Anti-cGMP antibody was obtained from PerkinElmer and GenScript ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were detected via ECL reagents (PerkinElmer).