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Citations for PerkinElmer :
1 - 50 of 209 citations for Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 3G APOBEC3G Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The chemiluminescence catalytic activity was assessed by EnVision Multilabel plate reader (PerkinElmer) using a liquid auto-injection system and luminol substrate ...
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bioRxiv - Cancer Biology 2022Quote: Tritiated cGAMP was synthesized via a bio-catalytic reaction in which recombinant cGAMP synthase preactivated with herring DNA was incubated with [3H]-ATP (Perkin Elmer) and [3H]-GTP (Perkin Elmer ...
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bioRxiv - Neuroscience 2020Quote: ... POD-conjugated antibody labeling of mRNA was proceeded with TSA Plus DNP signal amplification (Perkin Elmer) and a subsequently labeling with anti-DNP-KLH-488 antibodies (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... then mRNA were submitted to library preparation using NextFlex Rapid Directional mRNA (PerkinElmer) and 100 ng input ...
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bioRxiv - Biophysics 2023Quote: ... The uptake reaction was terminated by filtering the samples through a 96 well glass fiber filter (Filtermat B – GF/B, Perkin Elmer) soaked in 1.5% poly(ethyleneimine ...
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bioRxiv - Biochemistry 2021Quote: ... Enzyme inhibition was then quantified using an Envision instrument (PerkinElmer). Data were normalized to maximal inhibition determined by 1 μM decanoyl-Arg-Val-Lys-Arg-chloromethylketone (Calbiochem #344930).
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bioRxiv - Cancer Biology 2023Quote: ... mRNA was isolated by NEXTFLEX® Poly(A) Beads (PerkinElmer), quality checked by RNA 6000 nano chip (Agilent ...
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bioRxiv - Biochemistry 2022Quote: ... and the change in tryptophan fluorescence associated with the exchange of guanyl nucleotides within the α subunit of Gt was measured for 1200 s with a FL 6500 Fluorescence Spectrometer (PerkinElmer, Waltham, MA). Excitation and emission wavelengths were set at 300 nm and 345 nm ...
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bioRxiv - Developmental Biology 2021Quote: ... nord mRNAs were visualized using TSA Plus Cyanine 5 (NEL745001KT, PerkinElmer). Dpp and GFP expression after nord FISH were detected as described below in the Immunohistochemistry and imaging section.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Assays were conducted in UniFilter-96 GF/B plates (PerkinElmer) with 1 ug of membranes per well in a final volume of 300 μl binding buffer consisting of 20 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... 200 µl of buffer B containing 86Rb+ (PerkinElmer, Waltham, MA) at approximately 4.5 nCi was added at time zero ...
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bioRxiv - Immunology 2023Quote: ... and an ELAST enzyme-linked immunosorbent assay (ELISA) amplification system (PerkinElmer, Inc.) was also used ...
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bioRxiv - Microbiology 2020Quote: ... the mixtures were spotted onto filtermat B (Perkin Elmer, cat#1450-521) soaked in 0.5% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The reactions were terminated through filtration with GF/B filters (Perkin Elmer) using a 96-well plate harvester (Brandel) ...
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bioRxiv - Cell Biology 2019Quote: ... 6 μM mRNA and 0.3 μM [35S]-fMet-tRNAfMet ([35S]-methionine from Perkin Elmer). An aliquot (4.5 μl ...
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bioRxiv - Biochemistry 2024Quote: Fluorescence was measured using the Luminescence Spectrometer L 50 B from Perkin Elmer. Spectra were recorded with an excitation wavelength of 280 nm using a 5 x 10 mm cell at 25 °C with excitation and emission slits of 3 and 5 nm ...
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bioRxiv - Microbiology 2019Quote: ... All enzyme concentrations were determined by active site titration [53,54] using [14C]-alanine (Perkin Elmer) and [14C]-threonine (American Radiochemicals).
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bioRxiv - Cancer Biology 2024Quote: 12 RNA libraries were prepared with the NextFlex Rapid Directional mRNA-Seq kit (Perkin Elmer) with polyA+ mRNA enrichment ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The reaction mixture was then filtered over a GF-B filter plate (Perkin Elmer) followed by four quick washes with ice-cold 50 mM Tris HCl ...
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bioRxiv - Biophysics 2023Quote: ... Filter plates were dried at 96℃ before scintillation sheet (MeltiLex B/HS, Perkin Elmer) was melted on to the filter ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Plates were then filtered on GF/B filter plates precoated with 0.1% PEI (PerkinElmer #6005177) and washed with 1.2mL wash buffer and fan dried ...
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bioRxiv - Genetics 2023Quote: RNA-seq libraries were generated with the NEXTflexTM Rapid Directional mRNA-Seq Kit (NOVA-5138, Perkin Elmer). 10 µg of total RNA was used as input for all libraries ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL immunoprecipitated enzyme were mixed with 300 μM synthetic peptide and 50 μM [14C]-acetyl-CoA (PerkinElmer) in acetylation buffer (50 mM HEPES pH 8.5 ...
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bioRxiv - Microbiology 2021Quote: ... the resulting viral particles were collected and titrated in VeroE6 cells by enzyme luminescence assay (Britelite plus kit; PerkinElmer), as described previously [91].
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bioRxiv - Immunology 2022Quote: ... and the resulting viral particles were titrated in TZMbl cells using an enzyme luminescence assay (britelite plus kit; PerkinElmer) as described previously [82] ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from standard cultures were prepared according to manufacturer’s protocol using the NextFlex Combo-Seq mRNA/miRNA kit (PerkinElmer), to capture both the mRNA and miRNA in the samples ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was isolated from 150ng total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer, Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit ...
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bioRxiv - Molecular Biology 2019Quote: ... Micrographs were captured with a digital camera mounted on a Leica DM6000 B upright microscope with either 10X or 40X objectives using Volocity Imaging software (version 6.3.0 PerkinElmer).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein-bound [3H]ryanodine was separated by filtration through polyethyleneimine-treated glass filters (Filtermat B; PerkinElmer, Waltham, MA) using a Micro 96 Cell Harvester (Skatron Instruments ...
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bioRxiv - Molecular Biology 2020Quote: ... A LANCE Ultra TR-FRET kinase assay was used for measurements of enzyme activities that included an-Ulight labeled (Thr-1342) peptide substrate (catalog #TRF0130-M, PerkinElmer) and a Europium-labeled anti-phospho peptide antibody (catalog # TRF0218-M) ...
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bioRxiv - Biochemistry 2022Quote: ... Enzyme is incubated with 25 μM compound or 1.25% DMSO in 384-well plates (Perkin Elmer Proxiplate 384F Plus #6008269) for 30 min before the addition of an equal volume of substrate (800 nM final concentration ...
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bioRxiv - Microbiology 2023Quote: ... ε-NAD was used as a fluorogenic substrate to report ThsA enzyme NADase activity by monitoring increase in fluorescence (excitation 300 nm, emission 410 nm) using EnSpire Multimode Plate Reader (PerkinElmer) at 37 °C ...
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bioRxiv - Cell Biology 2019Quote: RNA was isolated from the ZEB1 CEnC lines and RNA-seq libraries were prepared using the KAPA mRNA HyperPrep Kit using an automated liquid handler (Janus G3 – PerkinElmer) according to manufacturer’s instructions at the UCLA Institute for Quantitative and Computational Biology ...
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bioRxiv - Genetics 2021Quote: ... 100 ng of polyA-selected mRNA from each meiotic stage was prepared using the NEXTFLEXTM Rapid Directional RNA-seq Kit (NOVA-5138-08; PerkinElmer) according to the provided manual ...
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bioRxiv - Cell Biology 2019Quote: RNA was isolated and RNA-seq libraries were prepared using the KAPA mRNA HyperPrep Kit with an automated liquid handler (Janus G3 – PerkinElmer) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared from mRNA samples using NEXTFLEX® Rapid Directional RNA-seq Kit 2.0 (Perkin Elmer, Austin, Texas, USA). Quality of library preparation was assessed on a 2100 Agilent Bioanalyzer ...
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bioRxiv - Neuroscience 2021Quote: ... circulating levels of minocycline were measured from plasma collected in a sub-set of subjects at the end of the experiment using a commercially available tetracycline enzyme immunoassay (Perkin Elmer, Akron ...
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bioRxiv - Plant Biology 2020Quote: ... 10.5 μg of gDNA was digested with the restriction enzymes EcoRV and XbaI from New England Biolabs (http://www.neb.com) and blotted onto a nylon membrane (GeneScreenPlus; Perkin Elmer, http://www.perkinelmer.com) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... 1 µCi 3H-SAM and ∼1 µg of enzyme) for 1 hour at 30° C following which they are transferred to FlashPlates (Perkin Elmer) and incubated for 30 min for peptide capture ...
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bioRxiv - Biochemistry 2020Quote: ... The 500 nM annealed DNA substrates were then radiolabeled in PNK buffer with PNK enzyme and radioactive ATP [γ32P] from Perkin Elmer at 37°C for 1 hr then diluted in TE buffer (10 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... filtered through a 0.45-µm-pore size filter and quantified for viral Cap24 content using enzyme-linked immunosorbent assay (ELISA) (Perkin Elmer, # NEK050A) as previously published (41 ...
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bioRxiv - Neuroscience 2023Quote: ... 50 μl of media from each sample and 50 μl of the enzyme and substrate mix was pipetted in a well of the imaging plate (PerkinElmer, 6055300). The plate was briefly mixed and incubated for 1 h at RT avoiding exposure to light ...
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bioRxiv - Physiology 2020Quote: ... cDNA was amplified using the NuGEN Ovation V2 system and all purified samples were judged to be of high quality and sufficient quantity for next generation mRNA sequencing using the LabChip method (Perkin Elmer). TruSeq DNA Nano gel free libraries (350bp insert ...
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bioRxiv - Cancer Biology 2021Quote: ... For each PI3K isoform a 3 µL mixture (1:1) of enzyme/lipid substrate solution was added to a 384 well white assay plate (Perkin Elmer #6007299) containing 50 µL of test compound or DMSO only for untreated controls ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences). Light emission was measured with an LB942 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (PerkinElmer Life Sciences). Light emission was measured with an LB 942 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminomete (Berthold Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB941 TriStar luminometer (Berthold Technologies) ...
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bioRxiv - Immunology 2021Quote: ... HRP enzyme activity was determined after the addition of a 1:1 mix of Western Lightning oxidizing and luminol reagents (Perkin Elmer Life Sciences). Light emission was measured with a LB942 TriStar luminometer (Berthold Technologies) ...