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Citations for PerkinElmer :
1 - 50 of 449 citations for Anti PI 4 kinase II beta Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... immunoprecipitates were analyzed for kinase activity in presence of phosphatidylinositol (PI) and γ-32P-ATP (PerkinElmer, Santa Clara, CA) followed a published method 91 ...
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bioRxiv - Cell Biology 2023Quote: ... PI images were collected using Operetta (Perkin Elmer) high content microscope and PI intensity data were evaluated using the Harmony Software 4.1 of PerkinElmer.
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bioRxiv - Cell Biology 2023Quote: ... A Micro Beta liquid scintillation counter (PerkinElmer) was used to quantify radiation (counts-per-minute ...
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bioRxiv - Cell Biology 2023Quote: ... A Micro Beta liquid scintillation counter (PerkinElmer) was used to quantify radiation (counts-per-minute ...
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bioRxiv - Physiology 2022Quote: ... and a beta counter (Perkin Elmer, Boston, MA, USA). Results are expressed as % of total 14C counts per mg tissue.
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bioRxiv - Evolutionary Biology 2019Quote: ... animals were incubated overnight with peroxidase-conjugated antibodies at 4°C (anti-DIG-POD, Roche 1:500 dilution and anti-DNP, Perkin Elmer, 1:200 dilution) followed by the amplification of the signal with fluorophore-conjugated tyramides (1:100 TSA reagent diluents ...
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bioRxiv - Cell Biology 2021Quote: AlphaLISA® SureFire® Ultra™ cellular kinase immunoassays were used to measure endogenous kinase activity of p-mTOR (Ser2448) (PerkinElmer, #ALSU-PMTOR-C500), p-ERK 1/2 (Thr202/Tyr204 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... anti-cGMP antibody (PerkinElmer anti-cGMP antibody ...
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bioRxiv - Cell Biology 2021Quote: ... PI+/total cells over time were quantified with using the Harmony Analysis Software (PerkinElmer).
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bioRxiv - Evolutionary Biology 2022Quote: ... a given histidine kinase was first autophosphorylated with [γ-32P]-ATP (Perkin Elmer) for 90 minutes at 30 °C to drive autophosphorylation and then mixed at a 1:4 molar ratio with a response regulator (1 μM EnvZ ...
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bioRxiv - Molecular Biology 2023Quote: ... Probes were radiolabelled with 32P-ATP by poly nucleotide kinase (PNK) (PerkinElmer PNK) for 1 hour at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Anti-cGMP antibody was obtained from PerkinElmer and GenScript ...
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bioRxiv - Genomics 2020Quote: ... These steps were repeated starting from blocking for the second staining with primary antibody rat anti-CD8a 1:300, secondary antibody goat anti-rat (MP-7444, Vector) and opal 690 (fp1497, PerkinElmer) and the third staining with rat anti-CD45 1:1,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-DNP-HRP antibody (1:1000, Perkin Elmer) and tyramide signal amplification (Perkin Elmer) ...
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bioRxiv - Developmental Biology 2022Quote: ... The anti-DNP HRP antibody (Perkin Elmer, FP1129) was used 1:200 in blocking solution and embryos were incubated overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... primary antibodies followed by HRP-conjugated secondary polymer (anti-rabbit, anti-mouse [PerkinElmer]) and HRP-reactive OPAL fluorescent reagents (PerkinElmer) ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA probes were 5’-phosphorylated with T4 polynucleotide kinase (PNK, TOYOBO) and [γ-32P] ATP (PerkinElmer). Northern blotting of tRNAs was performed using PerfectHyb (Toyobo ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the kinase was autophosphorylated for 1 h at 30 °C with [γ-32P]ATP (Perkin Elmer) before being combined with RRs at a 1:4 ratio (10-μl reactions contained 1 μM EnvZ and 4 μM RR) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... [3H]-α,β-methylene ATP concentrations were checked on Tri Carb scintillation beta counters (PerkinElmer, USA). Data analysis was conducted using GraphPad Prism v9 ...
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bioRxiv - Developmental Biology 2019Quote: ... or HRP-conjugated anti-dinitrophenol antibody (Perkin Elmer, FP1129) were used to detect labeled probes by fluorophore deposition (Fluorescein or Cyanine 3 ...
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bioRxiv - Microbiology 2021Quote: ... an HRP-conjugated goat anti-rabbit secondary antibody (PerkinElmer) and the Western Lightning ECL Pro kit (PerkinElmer) ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1:400 anti-6xHis terbium antibody (PerkinElmer #61HI2TLF) in 10μL of buffer containing 20mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1:400 anti-6xHis terbium antibody (PerkinElmer #61HI2TLF) in 10 uL of buffer containing 20 mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... Blots were probed overnight at 4 °C with indicated antibodies and developed by chemiluminescence (Perkin Elmer).
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bioRxiv - Molecular Biology 2020Quote: mTOR kinase activity was monitored by LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET, PerkinElmer) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase-conjugated goat anti-rabbit polyclonal and anti-mouse antibodies were purchased from PerkinElmer Life Sciences (Boston ...
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bioRxiv - Microbiology 2019Quote: ... USA) Horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse antibodies were purchased from PerkinElmer Life Sciences (Boston ...
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bioRxiv - Bioengineering 2022Quote: ... plates were incubated with anti-mouse IgG-Europium antibody (PerkinElmer) for 1 hour ...
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bioRxiv - Genomics 2020Quote: ... secondary antibody goat anti-rat and Opal 520 (fp1487, PerkinElmer). After the third staining ...
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bioRxiv - Neuroscience 2022Quote: ... Flu-positive cells were visualized with anti-FITC antibody (PerkinElmer, #NEF710001EA ...
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bioRxiv - Microbiology 2022Quote: ... Extracted crRNAs were end-labeled with T4 Polynucleotide Kinase in a reaction containing γ-[32P]-ATP (PerkinElmer, MA, USA), and resolved on an 8% Urea PAGE ...
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082), and 50 nM LANCE Ultra Ulight-Streptavidin (PerkinElmer TRF0102 ...
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bioRxiv - Neuroscience 2023Quote: ... and HRP-labeled goat anti-rabbit antibody (1:500, PerkinElmer, NEF812001EA) for secondary detection ...
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bioRxiv - Plant Biology 2023Quote: ... The secondary antibody (Anti-Rabbit IgG [Goat], HRP-Labeled, PerkinElmer NEF812001EA) was 1:10000 in 5% TBST-milk.
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bioRxiv - Developmental Biology 2023Quote: ... and anti-DNP HRP-conjugated antibody (Perkin Elmer, Cat# FP 1129) followed by tyramide signal amplification (Akoya Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP-conjugated secondary anti-rabbit and anti-mouse IgG antibodies were obtained from PerkinElmer (Waltham, MA, USA). Vectashield antifade mounting medium with 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2021Quote: ... and dead cells (stained by PI+) were imaged every hour for the indicated time with the Operetta CLS High-Content microscope (Perkin Elmer) at 40x Air/0.6 NA ...
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bioRxiv - Plant Biology 2023Quote: ... or the CRI Maestro II (PerkinElmer, USA) live imaging system for GFP imaging in 35S:GFP Arabidopsis seedlings.
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bioRxiv - Neuroscience 2021Quote: ... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 0.2 µM kinase was incubated with 7 µM substrate and 0.2 mM ATP containing 0.45 mCi [32P]-γ-ATP/mL (Perkin Elmer) in kinase buffer (50 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... anti-rabbit or anti-mouse secondary antibodies conjugated to horseradish peroxidase were used at 1:10,00 dilution (PerkinElmer). Immunoblots were developed using PerkinElmer Western Lightning Plus-ECL and imaged with a GE Healthcare Amersham Imager 600 ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then incubated with an anti-DNP-AP antibody (PerkinElmer) (1:500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Table 9) and the compounds added to plates using the then kinase compounds added by the Sciclone ALH3000 robot (Perkin Elmer). Organoids were imaged using the Cytation5 (BioTek ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with a 32P-labelled oligodeoxyribonucleotide probe complementary to the target RNAs (32P-labelled using T4 polynucleotide kinase and [g 32P]-ATP [Perkin Elmer]) overnight in 37°C in SES1 buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
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bioRxiv - Immunology 2019Quote: ... Blood samples were collected and diluted in Liquid Scintillation Counting cocktail (Ultima Gold) and measured using a Scintillation counter (Beta Counter MicroBeta2 System, Perkin Elmer).
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bioRxiv - Microbiology 2020Quote: ... 20 μCi of radioactive Pi solution (10 μL from a 2 mCi K2H32PO4 at a concentration of 2 mM of K2H32PO4, PerkinElmer cat. no. NEX055) was added to 1 mL of cell suspension ...