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Citations for PerkinElmer :
351 - 400 of 1153 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade C 16055 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Complexes were incubated for 1h at 37°C and luminescence released by the [14C]-RITA-excited beads was measured in standard microplate reader (Perkin Elmer).
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bioRxiv - Cell Biology 2021Quote: ... was monitored every 5 min for 2 h at 37°C using a fluorescent plate reader (EnSpire Multimode Plate Reader, Perkin Elmer). Samples were assayed in technical triplicates and in biological replicates as indicated in the figure legend.
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bioRxiv - Cell Biology 2020Quote: ... and recorded on a 37°C heated stage of Nikon Ti inverted microscope using Ultraview spinning disk confocal system (Perkin Elmer). For the TEM opening ...
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bioRxiv - Developmental Biology 2022Quote: ... The sections were prehybridized and hybridized at 45°C for 4 hours in 50% (Vol/Vol) formamide hybridization buffer containing 35S-labeled antisense RNA probes (Perkin Elmer). RNase A-resistant hybrids were detected by autoradiography ...
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bioRxiv - Developmental Biology 2022Quote: ... For signal detection sections were incubated with the channel specific HRP for 20 min at 40 °C and incubated with the respective fluorophores (Perkin Elmer: Fluorescein ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed and incubated at 37 °C in phosphate buffered saline (PBS) containing 2 μCi/ml [3H]-glycine (1.6 TBq/mmol; PerkinElmer Life Sciences) isotopically diluted to reach 10 μM final glycine concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated at restrictive temperature (37°C) for 3 h and finally transfer to 384-well CellCarrier plates (PerkinElmer, 6007550) for imaging on an Opera QEHS high-content screening microscope (PerkinElmer) ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Aq-NF-κB or GST-Aq-NF-κB-ALA C-terminal peptides and 5 μCi [γ-32P] ATP (Perkin Elmer) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: The membrane was hybridized overnight at 50°C with DNA oligo probes labeled with [gamma- 32P]-ATP (6000 Ci/mmol) (Perkin Elmer). 20 pmol of the DNA oligo was mixed with 7 μl of [gamma- 32P]-ATP ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 16–18 hours at 37°C before Fluc activity in cell lysates was detected using a plate luminometer (EnSpire 2300 Multilabel reader by Perkin Elmer) and the Bright-Glo Luciferase Assays System (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... Imaging was performed at 37°C and 3% CO2 using an Opera Phenix™ high-content confocal microscopy system (Perkin Elmer). Images were acquired at 40x magnification using a 425 nm laser for excitation of CFP and emissions detected at 435-515 nm (CFP ...
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bioRxiv - Microbiology 2019Quote: ... 100 μL each of sample and Reagent B were combined in each well of a culture plate and the plate was incubated at 60 °C for 10 minutes before reading absorbance at 432 nm on a Wallac 1420 Victor2 multilabel plate reader (Perkin Elmer). A clean plate was used for each assay ...
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Surveying the landscape of tRNA modifications by combining tRNA sequencing and RNA mass spectrometrybioRxiv - Biochemistry 2019Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42 °C with 4 pmol DNA probes radiolabeled with [γ-32P]ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... Liquid cultures were incubated for seven days at 29 °C in the dark and the bioluminescence signal was measured daily with an EnVision plate reader (Perkin Elmer) three times for three seconds per well ...
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bioRxiv - Microbiology 2021Quote: ... 200 µl of the diluted cultures were transferred onto 96-well plate and OD490 at 37 °C was measured at fixed interval using Victor X4 plate reader (Perkin Elmer). For manual growth curve and CFU enumeration ...
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bioRxiv - Microbiology 2022Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42°C with 4 pmol probes (Table S3) that were radiolabeled using [γ-32P] ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 200 μL of these dilutions were then pipetted into opaque 96-well microplates and incubated at 30°C in a Victor X3 multilabel microplate reader (Perkin Elmer) that periodically measured absorbance (OD600 ...
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bioRxiv - Synthetic Biology 2022Quote: ... cells were incubated with the AAVs for 24 hours at 37°C and assayed with Britelite plus (Perkin Elmer, cat#6066766) following the manufacturer’s protocol.
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bioRxiv - Biophysics 2023Quote: ... and their proliferation was recorded at 37 °C with a Nikon Ti inverted microscope using an Ultraview spinning disk confocal system (Perkin Elmer). For determining TEM opening ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then maintained at constant 37°C for 36 hours before moving to an Opera Phenix Plus (Revvity Perkin Elmer, at the UCSC Chemical Screening Center RRID SCR_021114) ...
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bioRxiv - Microbiology 2023Quote: Growth of bacterial cultures to study phage infection or toxicity of SAMase was carried at 37°C in a 96-well format using EnSpire Multimode Plate Reader (Perkin Elmer). Overnight bacterial cultures were diluted 100-fold in LB medium with appropriate antibiotics and grown at 37°C in 10 mL of LB ...
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bioRxiv - Neuroscience 2023Quote: ... The choroid plexus was subsequently swiftly rinsed in cold isotope-free HEPES-aCSF containing 2 mM ouabain (4°C) and transferred to a scintillation vial and dissolved in 100 μl Solvable (6NE9100, Perkin Elmer). For the light and dark phase influx experiment ...
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bioRxiv - Biochemistry 2023Quote: ... Binding reactions were incubated for 2 hours and then terminated by washing the solutions over a PEI-coated GF/C filter using a cell harvester (Perkin Elmer) followed by wash steps using ice-cold buffer [50 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... Filter-bound [3H]VUF15485 was measured by adding 25 µL Microscint-O per well to the dried GF/C-plate and radioactivity was consecutively quantified using the Wallac Microbeta counter (Perkin Elmer). The sigmoidal dose-dependent displacement curves of [3H]VUF15485 binding by unlabelled ligands were analysed using Graphpad Prism 8 ...
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bioRxiv - Immunology 2023Quote: ... B cells were lysed in water by osmotic pressure and were transferred from 96 well plates to glass fiber filtermat (8×12 Filtermat A, GF/C, 100/pk, #1450-421, Perkin Elmer). The filters were rinsed with water and sealed in plastic bags with a scintillation cocktail (OptiScint® LLT ...
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bioRxiv - Neuroscience 2023Quote: ... Bioluminescence was recorded every 30 min in DD at 25°C and 65% humidity using a TopCount Multiplate Reader (Perkin Elmer). An sCMOS camera (DMK33UX178 ...
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bioRxiv - Biochemistry 2023Quote: ... Incubations were done at room temperature (24°C) for 4h and the end-point fluorescence reading obtained with a multimode microplate reader (Envision or EnSight, Perkin Elmer) equipped with excitation 405 nm and emission 535 nm filters ...
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bioRxiv - Biochemistry 2023Quote: ... Each binding reaction was allowed to reach equilibrium at 30 °C for 5 min prior to fluorescence polarization measurements in a Victor X5 Multilabel Plate Reader (Perkin Elmer). Each Kd measurement was calculated according to our previous work (45) ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions are incubated at 30 °C for 20 min prior to luminescence measurement for 10 s on the Victor X5 Multilabel Plate Reader (Perkin Elmer) by addition of 75 μl Renilla luciferase substrate (Promega) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The 96-well plate was incubated for 24h at 37 °C in a Victor3 microtiter plate reader (PerkinElmer, Waltham, MA, USA), and absorbance was measured every three minutes ...
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bioRxiv - Cell Biology 2019Quote: ... detection of proteins from the membranes was performed with Western Lightning ECL Pro solution (Perkin Elmer, #NEL121001EA).
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bioRxiv - Immunology 2021Quote: Data processing was done with Elucidator software (Protein Expression Data Analysis System (Ver. 4.0.0.ror2.31, Ceiba Solutions/ PerkinElmer) for label-free quantitation based on comparison of MS1 feature volume ...
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bioRxiv - Immunology 2019Quote: ... Cells were then distributed (80 µg of proteins per well) in a 96-well microplate (Optiplate, PerkinElmer). BRET2 between RLuc8 and GFP10 was measured 1 min after addition of 5 µM coelenterazine 400a/Deep blue C (Gentaur) ...
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bioRxiv - Genomics 2022Quote: ... For 6HIS-tagged proteins a 10 μl mix of 2.5 μg/ml Ni-NTA acceptor beads (PerkinElmer) and 10 μg/ml streptavidin donor beads was used ...
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bioRxiv - Cell Biology 2022Quote: ... For the host protein response screen 10,000 cells/well were seeded on 96 well microplates (Perkin Elmer, CellCarrier-96 Ultra Microplates ...
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bioRxiv - Cancer Biology 2024Quote: Leucine incorporation into proteins was assayed using 3H-Leucine (cat. # NET460001MC) obtained from Perkin Elmer (Waltham, MA), as described in a previous publication [33].
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bioRxiv - Cell Biology 2023Quote: Pulse-chase experiments using 9.8 Mbq per dish of EASY-TAG EXPRESS Protein labeling mix [35S] (PerkinElmer) and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... Transformation of yeast cells (82) and in vivo labeling of iron-binding proteins with 55FeCl3 (Perkin Elmer) and immunoprecipitation of 55Fe/S proteins and scintillation counting were performed as published for yeast (59 ...
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bioRxiv - Cell Biology 2024Quote: ... Dissolved gel samples or resuspended protein samples were added to LSC vials with LSC cocktail (#6013319, PerkinElmer) before being processed by PerkinElmer Tri-Carb 4910 TR liquid scintillation analyzer ...
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bioRxiv - Bioengineering 2024Quote: Protein screening was performed using the Opera Phenix High-Content High-Throughput (HCHT) screening system (PerkinElmer, USA). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: 1 μl ml-1 of 0.1 mCi ml-1 14C glucose (PerkinElmer, Cat# NEC042V250UC, Waltham, MA, USA) was added to differentiation medium 48 h before the end of the experiments ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5% Sarkosyl, 2.5 mM MgCl2, 0.5 mM DTT, 0.05#mM biotin-A/C/G/UTP [Perkin Elmer], 0.4 u/μl RNase inhibitor). The total RNA was isolated with Trizol LS (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: PDE activity was measured at 30°C with the two-step method described by [48] using [3H]cGMP (Perkin Elmer, MA, USA). Aliquots of eluted fraction from SEC were incubated in 60 mM HEPES pH 7.2 assay buffer containing 0.1 mM EGTA ...
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bioRxiv - Neuroscience 2021Quote: Live-cell imaging was performed at 37°C and with 3% CO2 using an Opera Phenix™ high content confocal microscopy system (Perkin Elmer). One hour prior to imaging ...
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bioRxiv - Immunology 2020Quote: ... The pyroptotic process was monitored at 34°C and 7.5% CO2 using a fully automated spinning disk confocal microscope (PerkinElmer Technologies, OPERA QEHS) with a 40x water immersion objective (Aulner et al. ...
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bioRxiv - Immunology 2020Quote: ... and allowed to refold at 18°C for 24 h in a Streptavidin-coated scintillation microplate (Flashplate PLUS, Perkin Elmer, Boston, MA). Dissociation was initiated by adding excess unlabeled β2m and placing the microplate in a scintillation counter (TopCount NXT ...
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bioRxiv - Plant Biology 2020Quote: ... The reaction was incubated for four hours at 37°C and the results were visualised using the LabChip GX Touch 24 (PerkinElmer, Waltham, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane mixture was then filtered over a 0.3% PEI pre-treated GF-B/C plate (#6005174, Perkin Elmer, Waltham, MA, USA) with a cell harvester system ...
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bioRxiv - Molecular Biology 2024Quote: ... This RNA unwinding assay was performed at 37 °C in 384-well plates using a Victor Nivo plate reader (PerkinElmer, Basel, Switzerland). The excitation wavelength was set to 640 nM and the emission was measured at 685 nm ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent signals were monitored in real-time with a microplate reader for 24 h within a tissue culture incubator (37 °C, 5 % CO2) on black OptiPlate 96-well (PerkinElmer, MA, USA) under 20x objective ...