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Citations for PerkinElmer :
1151 - 1200 of 1373 citations for 6 Chloropyrazolo 1 5 a pyrimidine 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the GlyT1 probe was detected using anti-DIG-HRP (1:750) and tyramide amplification (Perkin Elmer TSA Cy3). This was followed by the immunohistochemistry protocol to amplify the GFP signal in Dact2-GFP retinas ...
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bioRxiv - Plant Biology 2019Quote: ... the membrane was incubated for 10 min at room temperature in the same buffer supplemented with 0.1 mM CaCl2 and 0.1 μM 45CaCl2 (13.90 mCi mg−1; Perkin Elmer), followed by 5 min rinsing with 50% ethanol ...
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bioRxiv - Microbiology 2021Quote: ... and 1 mL of the gas phase was analyzed on a gas chromatograph with flame ionization (Perkin Elmer), to determine the ethylene concentration in the sample.
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bioRxiv - Biochemistry 2022Quote: ... 100 μM cold ATP and 1 μCi [γ-35P] ATP (6000 Ci/mmol,10 mCi/ml, Perkin Elmer) with recombinant proteins (200-500 ng ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in horseradish peroxidase-conjugated anti-rabbit IgG (1:1000; cat# NEF812001EA, Perkin Elmer, Boston, MA) for 1.5 hours ...
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bioRxiv - Microbiology 2023Quote: DK1042 was cultured with isolate P9_31 in 1× LBGM or 0.1× LBGM in 24-well imaging microtiter plates (PerkinElmer). Wells were imaged on a Leica SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Pathology 2023Quote: Purified Vip3Aa (25 µg) and Cry1Ac (1 µg) toxins were radiolabeled with 0.5 mCi of NaI125 (Perkin Elmer) using chloramine T ...
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bioRxiv - Biochemistry 2023Quote: ... The plates were then incubated at room temperature for 1 h and read on an Envision 2104 (PerkinElmer) using the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cell mixtures were incubated for 1 hour at 37°C with 0.5% v/v glusulase (Perkin Elmer, NEE154001EA) to digest cell walls of vegetative cells and asci ...
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bioRxiv - Biophysics 2021Quote: ... A 50 μl reaction mixture contains 1.6 mM [14C]-G3P (specific activity at 129.3 mCi mmol-1, PerkinElmer, USA), 0.8 mM CDP-DAG (Avanti ...
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bioRxiv - Developmental Biology 2021Quote: ... Perkin-Elmer) coupled with fluorogenic substrate Alexa Fluor 594 tyramide for 30 s (1:200, TSA kit; Perkin Elmer). F-actin was stained with rhodamine-conjugated phalloidin (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... Oocytes were lysed with 1M NaOH and 1%SDS before the addition of 3 mL scintillant (Optiphase HisSafe, PerkinElmer), and l-[3H]glutamate transport was measured using a MicroBeta TriLux scintillation counter (PerkinElmer).
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bioRxiv - Microbiology 2020Quote: ... programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag, Perkin Elmer). Reactions were incubated at 30 °C for 30 min before adding L-methionine (1 mM final ...
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bioRxiv - Developmental Biology 2019Quote: ... slides were washed in TNT and treated with Fluorescein Amplification Reagent (Perkin Elmer, 1:50 in Plus Amplification Diluent) for 15 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: 2.5 × 105 THP-1 cells were seeded on 24-well optical bottom tissue culture plates (Perkin Elmer, 1450-606), treated with 100nM phorbol 12-myrystate-13-acetate (PMA ...
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bioRxiv - Immunology 2021Quote: ... of the abdominal region was measured (exposure=1 sec., F/stop= 1.2, FOV=E, binning=medium, emissions filter=open) using Living Image software (PerkinElmer). Survival of the mice was recorded until 20 days after infection.
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bioRxiv - Cancer Biology 2021Quote: ... Slides were washed 3 times in PBS and incubated with tyramide Cy3 1:150 in amplification buffer (Perkin Elmer) for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... ATP was measured in supernatants according to the protocol described by the ATPlite 1 step Luminescence Assay System (PerkinElmer). Luminescence readings were obtained from a Bioteck Synergy HT plate reader ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:1500 ...
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bioRxiv - Cancer Biology 2022Quote: ... were were dispensed per well 1 h prior to imaging at Cytation5 image cytometer or Opera Phenix (Perkin Elmer) confocal screening microscope.
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bioRxiv - Molecular Biology 2021Quote: ... The medium was removed and 125I-GLP-1(7-36)NH2 (60 pM) or 125I-GCG (40 pM) (PerkinElmer) and increasing concentrations unlabeled tirzepatide or peptide 20 were added for overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated in a goat anti-rabbit horse radish peroxidase-conjugated IgG (1:200, NEF812001EA, PerkinElmer, Waltham, MA) for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... Two 100-μl fractions were collected in 50 μl of 1.5 M Tris-HCl (pH 8.0) and 1 ml of Ultima Gold LLT scintillation cocktail (Perkin Elmer). Radioactivity was measured using a liquid scintillation analyser (Packard).
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again and incubated with the appropriate Opal tertiary antibody (1:100 in amplification diluent, Perkin Elmer) for 60 min ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were incubated for 1 hour at room temperature before FRET was detected using an Envision plate reader (PerkinElmer). Emission of Lumi4TM-Tb cryptate was detected at 620 nm and emission of d2-conjugated IP1 at 665 nm ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Bioengineering 2021Quote: ... background luminescence was measured for each rat (1 sec exposure, field of view D, PerkinElmer IVIS, Waltham, MA, USA). Both knees were then injected with either 50 μL NanoLuc™ Luciferase (NL ...
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bioRxiv - Microbiology 2021Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer) and/or by determining their infection titers on target cells.
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bioRxiv - Microbiology 2021Quote: ... was used (at 1:20.000 dilution) for protein visualization in the presence of Western Lightning Plus-ECL enhanced chemiluminescence substrate (PerkinElmer). The WesternSure® Pre-stained Chemiluminescent Protein Ladder (Li-cor ...
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bioRxiv - Physiology 2022Quote: ... EP3R immunoreactivity was visualized by reaction with FITC-conjugated tyramide (1:50; SAT701001KT; Tyramide Signal Amplification FITC Systems, PerkinElmer) for 3 min ...
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bioRxiv - Microbiology 2022Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer). VSV-G pseudotyped HIV-1 (NL4.3 ...
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bioRxiv - Neuroscience 2022Quote: ... was diluted at 1 µg/mL in PBS and passively absorbed on multiwell plates (SpectraPlate-384 HB, Perkin Elmer) overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: The lipid composition of bacterial strains was determined after labeling with [1-14C] acetate (60 mCi/mmol; Perkin Elmer), [32P] phosphate (1 Ci/mmol ...
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bioRxiv - Neuroscience 2023Quote: ... pH 7.5/1 μM fluoxetine) containing 50 pM of the DAT ligand (125I) RTI55 (PerkinElmer Life Sciences, Boston, USA) for 60 min ...
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bioRxiv - Plant Biology 2023Quote: ... samples were incubated for 1h in 1:50 of a digoxigenin-tyramide signal amplification solution (TSA Plus DIG, PerkinElmer) prepared according to manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: ... cells were washed 3 times with wash buffer (PBS + 1 mM CaCl2 + 0.1% BSA) before adding Microscint-20 (PerkinElmer) for tritium concentration measurement using a TriLux MicroBeta2 plate reader (PerkinElmer) ...
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bioRxiv - Microbiology 2023Quote: ... normalized by cell OD600 (final OD=0.4) and diluted 1: 100 into 150 μl of fresh TSB media in flat-bottomed 96-well plates (PerkinElmer). The cultures were overlaid with 100 μl of mineral oil (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at 37 °C and imaged with the Opera Phenix High Content Screening System (#HH14000000, Perkin Elmer). Number of counted nuclei was determined with the image analysis software Harmony (PerkinElmer ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then separated on a HayeSep column (7’ HayeSep N 1/8’’ Sf; PerkinElmer Inc., Waltham, USA), followed by molecular sieve (9’ Molecular Sieve 13x 1/8’’ Sf ...
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bioRxiv - Cancer Biology 2023Quote: ... 4T-1-Luc3 tumors and metastases were monitored by bioluminescence imaging on an IVIS Lumina III System (Perkin Elmer) twice a week after tumor implantation ...
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bioRxiv - Biochemistry 2023Quote: ... 100 nM of WT FCE or mutant K286M was incubated with 1 μM of 32P-α-GTP (Perkin Elmer) in a 20 μL reaction containing 1x RNA capping buffer at room temperature for 1 minute ...
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bioRxiv - Bioengineering 2023Quote: We used hairless SKH-1 mice for live animal imaging on IVIS Spectrum In vivo Imaging System (Perkin Elmer). For ovalbumin quantification ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed three times with TNT buffer (TN buffer + 0.05% Tween-20) and incubated with FITC-tyramide diluted 1:100 in Amplification reagent (PerkinElmer) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... was added to the media at a 1:10,000 dilution and images were acquired using the Opera Phenix High-Content Screening System (PerkinElmer). Analysis was performed using Harmony 191 High-Content Imaging and Analysis Software (PerkinElmer ...
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bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...
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bioRxiv - Molecular Biology 2021Quote: ... Small RNA libraries were prepared from 1 ng of RNA using the NEXTflex Small RNA-seq Kit v3 (Perkin Elmer). The size distribution of the libraries was assessed on a Bioanalyzer with a DNA High Sensitivity kit (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... The radiolabeled probes (200 bp) were generated by PCR using (γ-32P) dCTP 0.1 mM (3000 Ci. mmol-1 PerkinElmer). The primes used were ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were detected by incubation with peroxidase-conjugated anti-mouse and anti-rabbit antibodies (1:10000, Jackson Immuno Research Laboratories) followed by reaction with ECL-Plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Biochemistry 2019Quote: RAW264.7 cells in 24-well plates were labeled by overnight incubation with DMEM containing 1% fetal bovine serum and 0.5 μCi/mL [3H]cholesterol (Perkin Elmer). The next day ...