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Citations for PerkinElmer :
451 - 500 of 639 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... We extracted RNA on an automated Chemagic 360 instrument using the CMG-1049 kit (Perkin Elmer, Hamburg, Germany), or by manual extraction using the QIAamp Viral RNA Mini Kit (QIAGEN ...
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bioRxiv - Immunology 2023Quote: ... Intracellular levels of cAMP were detected using the LANCE Ultra cAMP kit (#TRF0263; PerkinElmer Life Sciences, Waltham, MA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... an Alpha SureFire Ultra Multiplex Phospho-EGFR (Tyr1068) and Total EGFR Assay Kit (PerkinElmer MPSU-PTEGFR-K-HV) was used ...
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bioRxiv - Cancer Biology 2023Quote: ... Total nucleic acid was extracted from FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer). Percent tumor cellularity as a ratio of tumor to normal nuclei was verified against pathologist-derived assessment ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were built using the PerkinElmer NextFlex Rapid Directional RNA-Seq Kit 2.0 (PerkinElmer catalog # NOVA-5198-02) according to the manufacturer’s instructions and sequenced with a NovaSeq 6000 (SE75).
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bioRxiv - Genomics 2022Quote: ... was used for NGS library construction using a NEXTflex Rapid DNA-Seq Kit 2.0 (PerkinElmer, Inc., Waltham, MA). Exome regions were enriched via hybridization with an IDTexome panel (Integrated DNA Technologies ...
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bioRxiv - Plant Biology 2022Quote: ... RNA-Seq libraries were prepared from poly(A)-selected RNA samples using the NEXTFLEX Poly(A) Beads 2.0 and Rapid Directional RNA-Seq kit 2.0 (PerkinElmer) and then sequenced on the Illumina NovaSeq 6000 SP v1.5 (2×150 ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were lysed and the cAMP was measured using a LANCE ultra cAMP Detection Kit (Perkin Elmer) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... reverse transcription and subsequent cDNA amplification were performed using the NEXT-Flex smRNA- Seq Kit v3 (Perkin Elmer). Samples were subjected to 8% native PAGE (0.5X TBE ...
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bioRxiv - Cell Biology 2024Quote: ... except Illumina adapters provided with the kit were replaced with NEXTFLEX® Unique Dual Index Barcodes (Perkin Elmer, references NOVA-514150 ...
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bioRxiv - Molecular Biology 2024Quote: ... reverse transcription and subsequent cDNA amplification was performed using the NEXT-Flex smRNA-Seq Kit v3 (Perkin Elmer); cDNA library QC was performed by bioanalyzer before sequencing on an Illumina NovaSeq 6000 single-end for 100 cycles ...
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bioRxiv - Physiology 2024Quote: ... and size pattern is controlled with the DNA High Sensitivity Reagent kit on a LabChip GX (Perkin Elmer). Libraries are pooled at an equimolar ratio (i.e ...
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bioRxiv - Immunology 2024Quote: ... supernatants were collected and IL-1β levels were measured using the Human IL-1β HTRF kit (Cisbio – PerkinElmer), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... with a dsDNA High-Sensitivity Assay kit and LabChip GX Touch using a High-Sensitivity DNA chip (PerkinElmer). Libraries were then pooled at equimolar concentrations and quantified by qPCR using a KAPA library quantification kit (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... Perkin-Elmer) coupled with fluorogenic substrate Alexa Fluor 594 tyramide for 30 s (1:200, TSA kit; Perkin Elmer). F-actin was stained with rhodamine-conjugated phalloidin (1:200 ...
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bioRxiv - Developmental Biology 2022Quote: ... then the signal was revealed using Tyramide Signal Amplification (TSA) with Fluorescein TSA Plus Evaluation Kit (Perkin Elmer, MA).
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... with HRP goat anti-mouse IgG Ab (31430, 1:20,000) for 1 h and developed using a Western Lightening Enhanced Chemiluminescence kit (PerkinElmer) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... Probe binding was indicated by deposition of target-specific fluorophores at each location via TSA Plus Fluorescence kit (PerkinElmer), and sections were then counterstained with DAPI ...
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bioRxiv - Physiology 2021Quote: ... acylcarnitines were derivatized to their butyl esters and treated with the reagents of the NeoGram MSMS-AAAC kit (PerkinElmer). Their analysis was carried out on a Waters 2795/Quattro Micro AP liquid chromatography–tandem mass spectrometer (Waters ...
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bioRxiv - Neuroscience 2021Quote: ... Strand-specific libraries with unique barcodes were prepared using the NEXTFLEX Rapid Directional RNA-Seq kit 2.0 according to manufacturer’s instructions (Perkin Elmer). Libraries were pooled in equal molar amounts and sequenced on an Illumina HiSeq 2500 to obtain roughly 40 million reads per sample.
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bioRxiv - Microbiology 2021Quote: ... the resulting viral particles were collected and titrated in VeroE6 cells by enzyme luminescence assay (Britelite plus kit; PerkinElmer), as described previously [91].
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bioRxiv - Genetics 2022Quote: ... Amplified products were quantified with Quant-iT(tm) PicoGreen dsDNA Assay Kit on a VICTOR Multilabel Plate Reader (PerkinElmer) and pooled in equimolar ratios ...
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bioRxiv - Immunology 2022Quote: ... and the resulting viral particles were titrated in TZMbl cells using an enzyme luminescence assay (britelite plus kit; PerkinElmer) as described previously [82] ...
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bioRxiv - Neuroscience 2021Quote: The level of MMP-9 in plasma was measured with an AlphaLISA kit for the human protein (#AL3138, PerkinElmer). AlphaLISA signals were measured using an Ensight Multimode Plate Reader (PerkinElmer).
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bioRxiv - Microbiology 2021Quote: ... Pooled samples were size selected with the LabChip XT (LabChip XT DNA 300 Assay Kit; PerkinElmer, Seer Green, UK) and diluted to 26 pM for templating with the Ion OneTouch 2 system using the Ion PGM Template OT2 200 kit ...
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bioRxiv - Physiology 2020Quote: ... The fluorescence signal was developed with tyramide signal amplification (TSA) Plus Cyanine 3 System kit (PerkinElmer, Waltham, Massachusetts, USA) as described previously (Tulke et al. ...
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bioRxiv - Immunology 2021Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and LabChip GX Touch using a High Sensitivity DNA chip (Perkin Elmer). Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer) and/or by determining their infection titers on target cells.
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bioRxiv - Genomics 2021Quote: ... Insulin was quantified using the Insulin (human) AlphaLISA Detection Kit and the EnSpire Alpha Plate Reader (both Perkin Elmer) based on 1:10 and 1:200 dilutions for supernatant and insulin content ...
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bioRxiv - Pathology 2023Quote: ... Supernatant was discarded and DNA was extracted using the QuicPickTM SML Plant DNA purification kit according to the supplier’s instructions (Bio-Nobile, Turku, Finland) in an automated system (Caliper Zephyr, PerkinElmer). DNA was eluted from magnetic beads using 30 µL of elution buffer ...
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bioRxiv - Genomics 2023Quote: ... the converted DNA was then used for the library preparation using the NEXTFLEX® Bisulfite-Seq kit (Perkin Elmer) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... mRNA was isolated from 150ng total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer, Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were processed according to the NEXTFLEX® Small RNA-Seq Kit v4 with UDIs (PerkinElmer, #NOVA-5132-32) with a 1 µg starting input and 12 cycles of PCR ...
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bioRxiv - Immunology 2023Quote: RNA libraries were generated using the NEXTFLEX® Small RNA-Seq Kit (PerkinElmer, USA; Cat. No.: NOVA-5132-05). For each library ...
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bioRxiv - Cancer Biology 2022Quote: ... Luciferase activity was measured using Pierce Firefly Luciferase Glow Assay Kit according to manufacturer’s protocol using an EnVision multilabel plate reader (PerkinElmer). AlamarBlue (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... The above extracted microbial DNA was then subjected to amplification and sequencing of the V4 region of the 16S rRNA gene by using a NEXTflex 16S V4 Amplicon Seq kit 2.0 (PerkinElmer), and sequences were generated on the Illumina MiSeq platform (Illumina) ...
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bioRxiv - Microbiology 2022Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer). VSV-G pseudotyped HIV-1 (NL4.3 ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA from standard cultures were prepared according to manufacturer’s protocol using the NextFlex Combo-Seq mRNA/miRNA kit (PerkinElmer), to capture both the mRNA and miRNA in the samples ...
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bioRxiv - Cell Biology 2023Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and LabChip GX Touch using a High Sensitivity DNA chip (Perkin Elmer). Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Physiology 2023Quote: ... and its integrity was assessed using 5K / RNA / Charge Variant Assay LabChip and RNA Assay Reagent Kit (Perkin Elmer). Libraries were generated from 250 ng of total RNA as following ...
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bioRxiv - Molecular Biology 2024Quote: PDE8A activity assay was performed with recombinant PDE8A and cAMP detection using the LANCE Ultra cAMP kit (Perkin Elmer). To determine the most sensitive range for the assay ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction was performed using the Chemagic™ Prime Viral DNA/RNA 300 Kit H96 (Chemagic, CMG-1433, PerkinElmer). Detailed concentration and DNA extraction procedures are provided in the Supplementary materials (Materials and Methods ...
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bioRxiv - Immunology 2024Quote: ... with dsDNA HS (High Sensitivity) Assay Kit and LabChip GX Touch using a High Sensitivity DNA chip (Perkin Elmer). Libraries were then equimolarly pooled and quantified by qPCR using the KAPA library quantification kit (Roche) ...
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bioRxiv - Molecular Biology 2024Quote: ... The concentration of this first messenger was determined using the Lance Ultra cAMP kit (Perkin Elmer, Waltham, MA, US). The method consists of a time-resolved fluorescence resonance energy transfer (TR-FRET ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... IP1 levels were determined using the HTRF® IP-one assay kit as per manufacturer’s instructions and fluorescence measured at 615 and 665 nm using the Envision plate reader (PerkinElmer) after excitation at 340 nm ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by library preparation with the Next-Flex small RNA v.4 kit protocol (Perkin Elmer, NOVA-5132-06), in accordance to manufacture recommendations ...
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bioRxiv - Genomics 2024Quote: DNA was extracted from whole blood using the Chemagic™ DNA Blood 400 H96 kit (Perkin Elmer, CMG-1091). SNP genotyping was conducted using the Axiom™ Precision Medicine Research Array from Applied Biosystems (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2024Quote: ... An additional library was prepared in-house for the wild male with the NextFlex Rapid DNA-seq 2.0 kit (PerkinElmer), using half the recommended reaction volume ...
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bioRxiv - Bioengineering 2020Quote: ... Genomic DNA was isolated from a single colony using a JANUS Chemagic Workstation and Chemagic Viral DNA/RNA kit (PerkinElmer). Libraries were prepared with the Nextera XT DNA sample preparation kit (Illumina) ...
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bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity was measured using Pierce™ Firefly Luciferase Glow Assay Kit according to manufacturer’s protocol using an EnVision mulitlabel plate reader (PerkinElmer). AlamarBlue (ThermoFisher ...