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Citations for CisBio :
1 - 50 of 143 citations for Hemoglobin High Sensitivity Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HTRF detection reagents (cAMP Dynamic 2 kit, Cisbio) were added at the end of the incubation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Resuspended cells were stimulated at 37°C in their respective serum-free medium in 96-well half area opaque white plates before addition of HTRF lysis buffer and detection reagents (cAMP Dynamic 2 kit, Cisbio). The duration of stimulation and inclusion or not of the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Bioengineering 2021Quote: ... 2 μl Detection Buffer 1 (CisBio) was immediately added to the reaction mixture ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μl Detection Buffer 1 (CisBio) was immediately added to the reaction mixture ...
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bioRxiv - Bioengineering 2021Quote: ... to 49 parts Detection Buffer 2 (CisBio).
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bioRxiv - Biochemistry 2021Quote: ... to 49 parts Detection Buffer 2 (CisBio).
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bioRxiv - Neuroscience 2023Quote: ... phospho- and total ERK1/2 were determined using the two-plate adherent cell protocol (Phosph-ERK1/2 kit, Cisbio), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... or Insulin High-Range assay kits (Cisbio). For insulin content assays ...
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bioRxiv - Cell Biology 2022Quote: ... or an Insulin high range kit (Cisbio).
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bioRxiv - Microbiology 2022Quote: ... The reaction was quenched by the addition of 2 µl of 5M followed by the addition of 2 µl Detection Buffer 1 (Cisbio) to the reaction mixture ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Changes in intracellular cellular cAMP were determined using a modified 2-step High-Throughput Time Resolved Fluorescence (HTRF) cAMP kit (Cisbio Bioassay, Codolet, France). Confluent HEK or EnSC cultures in 6-well plates were down-regulated prior to stimulation and assayed in Krebs-Henseleit Buffer (KHB ...
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bioRxiv - Bioengineering 2021Quote: ... 16X SAH-d2 was prepared by adding one part SAH-d2 to 15 parts Detection Buffer 2 (CisBio).
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bioRxiv - Biochemistry 2021Quote: ... 16X SAH-d2 was prepared by adding one part SAH-d2 to 15 parts Detection Buffer 2 (CisBio). After 5 minutes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Supernatant insulin concentration was determined by HTRF (High Range Insulin kit, Cisbio). Results were normalised to those obtained with 11 mM glucose but no additional agonist ...
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bioRxiv - Neuroscience 2024Quote: Secreted insulin was detected using a commercially available insulin detection kit (PerkinElmer/Cisbio Bioassays, Bedford, MA) based on homogenous time-resolved fluorescence resonance energy transfer (HTRF ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Secreted insulin in the supernatant was analysed by HTRF (Insulin High Range kit, Cisbio) after dilution and normalised to the concentration in glucose-only treated wells.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cAMP determined by HTRF (cAMP Dynamic 2 kit, Cisbio) using a Spectramax i3x plate reader (Molecular Devices).
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bioRxiv - Cell Biology 2021Quote: ... Supernatants were collected to quantify the amount of IL-1β by HTRF according to the manufacturer’s protocol for the human IL-1β detection kit (Cisbio). In brief ...
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bioRxiv - Molecular Biology 2023Quote: IP1 accumulation assay was applied for detection of the downstream Gαq signal of MCHR2 using the IP-One HTRF kit (Cisbio). AD293 cells were cultured in DMEM/ high Glucose supplemented with 10% (v/v ...
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bioRxiv - Physiology 2023Quote: Inositol 1 phosphate (IP1) accumulation in hPC-PL was measured using the HTRF IP-One Gαq Detection Kit (Cisbio). This assay enables the direct pharmacological activity of Gαq - coupled receptors ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and homogenous time-resolved fluorescence (HTRF) reagents for cAMP (cAMP Dynamic 2) and insulin (Insulin High Range Assay) measurement were obtained from Cisbio. SNAP-Surface probes were obtained from New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were collected every min for insulin measurement using Insulin High Range Kits (Cisbio Assays, Bedford, MA) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: Total Yap and phospho-Yap were detected according to the two-plate protocol for adherent cells (Total Yap Cellular Kit, Cisbio 64YATPEG; phospho-Yap [Ser127] Cellular Kit, Cisbio 64YapPEG). The signal was detected by Synergy NEO (Biotek).
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bioRxiv - Pathology 2022Quote: Cardiomyocytes cAMP concentration was assessed with cAMP dynamic 2 kit (Cisbio, Saclay, France), according to the manufacturer instructions.
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bioRxiv - Physiology 2022Quote: ... GLP-1R or GIPR using the cAMP Dynamic 2 cAMP kit (Cisbio, France) as per manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... Samples were collected every min for insulin assay using Insulin High Range Kits (Cat# 62IN1PEG, RRID:AB_2890910, Cisbio Assays, Bedford, MA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 10μl of detection mixture containing Streptavidin-d2 (Cisbio 610SADLF, 1:200), PAb Anti Human IgG-Eu cryptate (Cisbio 61HFCKLA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and cAMP was determined by homogenous time-resolved fluorescence (HTRF, cAMP Dynamic 2 kit, Cisbio).
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bioRxiv - Cell Biology 2023Quote: ... 2 mM EGTA) and insulin content was measured by insulin ultra-sensitive assay kit (Cisbio). Within an experiment ...
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bioRxiv - Cell Biology 2024Quote: The induction of cAMP was quantified in EnSCs using a modified protocol of the High-Throughput Time Resolved Fluorescence (HTRF) cAMP Gαs dynamic kit (CisBio Bioassays, Codolet, France). A 2-step protocol allowed culture and stimulation of cells in 6-well plates and ensured cAMP levels were within the manufacturer’s detection range ...
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bioRxiv - Cancer Biology 2024Quote: ... The reaction was stopped by addition of detection buffer (Cisbio, 10 µL) containing (final concentrations) ...
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bioRxiv - Developmental Biology 2020Quote: Total Yap and phospho-Yap were detected according to the two-plate protocol for adherent cells (Total Yap Cellular Kit, Cisbio 64YATPEG ...
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bioRxiv - Neuroscience 2022Quote: The accumulation of cAMP was determined using a cAMP dynamic 2 kit (Cisbio, Bioassays, Bagnols sur-Cèze, France). Synaptosomes were incubated for 1 h at 37 ºC (0.67 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... cAMP concentrations were measured from cell lysates after cells were incubated for 5 minutes with synthetic agonists (10 μM) for FFAR2 (4-CMTB) or FFAR3 (AR420626) using the HTRF cAMP Dynamic 2 immunoassay kit (CisBio). Fluorescence was measured with a PHERAstar FSX plate reader (BMG Labtech ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then lysed using the supplied lysis buffer and assayed for ERK1/2 phosphorylation using the phospho-ERK (Thr202/Tyr204) Cellular Assay Kit (Cisbio). Plates were read using a Mithras LB 940 multimode microplate reader (Berthold Technologies ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and analysed for secreted insulin by HTRF (Insulin High Range Assay, Cisbio). Insulin secretion was expressed relative to that from cells stimulated with 11 mM glucose alone in the same experiment.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ERK1/2 phosphorylation was measured using the homogeneous time resolved fluorescence (HTRF)® Phospho-ERK (T202/Y204) Cellular Assay Kit (Cisbio Bioassays, Codolet, France) two-plate format in accordance with the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The assay was stopped by cell lysis and the response was measured by adding 20 µl/well detection solution (cAMP conjugate and lysis buffer (Cisbio) + 2.5% anti-cAMP cryptate conjugate (Cisbio ...
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bioRxiv - Neuroscience 2019Quote: ... All reactions contained 20 μM high purity S-adenosyl methionine (SAM, CisBio, Bedford, MA, USA) in COMT assay buffer (50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... the plate was washed twice with TagLite® (Cisbio, Codolet, France) and the extracellular SNAP-receptors were labelled with SNAP-Lumi4-Tb (100 nM ...
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bioRxiv - Biochemistry 2021Quote: ... the plate was washed twice with TagLite® (Cisbio, Codolet, France) and the extracellular SNAP-receptors were labelled with SNAP-Lumi4-Tb (100 nM ...
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bioRxiv - Cancer Biology 2024Quote: ... HTRF signal was read on an EnVision multimode plate reader (CisBio) in HTRF mode ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ERK1/2 phosphorylation was determined by HTRF (Cisbio Phospho-ERK [Thr202/Tyr204] cellular kit ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cAMP was quantified by HTRF (cAMP Dynamic 2, Cisbio), and responses were normalised to that of forskolin ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cAMP was then assayed by HTRF (Cisbio cAMP Dynamic 2) using a Spectramax i3x plate reader (Molecular Devices) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cAMP was then assayed by HTRF (Cisbio cAMP Dynamic 2) and concentration-response curves constructed with 3-parameter curve fitting in Prism 8.0.
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bioRxiv - Biochemistry 2024Quote: ... The 5 µl of reaction mixture was transformed to HTRF 96 well low volume plates (Cisbio) and the consumption of ATP was measured with ADP-Glo Kinase Assay kit (Promega Corporation ...
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bioRxiv - Bioengineering 2021Quote: ... EPIgenous Methyltransferase kit (Cisbio Bioassays) was used for the detection of methyltransferase activity as with nsp14.
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bioRxiv - Cell Biology 2024Quote: ... Transfected cells in black non-transparent 96-well plates were labeled in Tag-lite labeling buffer (Revvity Cisbio) with 100 nM SNAP-Lumi4-Tb for 1 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... followed by lysis and cAMP HTRF immunoassay (cAMP Dynamic 2, 62AM4PEB, Cisbio, Codolet, France) according to the manufacturer’s instructions ...