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Citations for CisBio :
1 - 50 of 140 citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... ERK phosphorylation was evaluated using a Homogenous Time-Resolved FRET (HTRF)-based Phospho-ERK (Thr202/Tyr204) cellular kit (Cisbio). Cells were lysed for 30 minutes with the lysis buffer provided and incubated for 2 hours with pERK1/2-specific antibodies conjugated with Eu3+-cryptate donor and d2 acceptor at recommended dilutions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then lysed using the supplied lysis buffer and assayed for ERK1/2 phosphorylation using the phospho-ERK (Thr202/Tyr204) Cellular Assay Kit (Cisbio). Plates were read using a Mithras LB 940 multimode microplate reader (Berthold Technologies ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ERK1/2 phosphorylation was measured using the homogeneous time resolved fluorescence (HTRF)® Phospho-ERK (T202/Y204) Cellular Assay Kit (Cisbio Bioassays, Codolet, France) two-plate format in accordance with the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... ERK1/2 phosphorylation was determined by HTRF (Cisbio Phospho-ERK [Thr202/Tyr204] cellular kit ...
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bioRxiv - Cell Biology 2023Quote: ... GSIS assays were performed using ultra-sensitive insulin HTRF assay kits (Cisbio) for insulin quantification.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ERK1/2 phosphorylation in HEK293 FPR cell systems was measured with the HTRF immunoassay from Cisbio, as described previously [37] ...
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bioRxiv - Cell Biology 2021Quote: ... or Insulin High-Range assay kits (Cisbio). For insulin content assays ...
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bioRxiv - Physiology 2020Quote: ... before assaying using a HTRF Mouse Serum Insulin Assay kit assay (Cisbio, France). Due to NC3R limits on blood sample volumes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Inhibition assays were performed using the HTRF KinEASE tyrosine kinase assay kit (Cisbio) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GTP binding assay was performed using a GTP Gi Binding Assay Kit (Cisbio) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ERK1/2 phosphorylation in HEK293 cells was measured with the HTRF (Homogeneous Time-Resolved Fluorescence) immunoassay from Cisbio, as described previously [23] ...
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bioRxiv - Biochemistry 2019Quote: ... The cAMP assay kit is from Cisbio (Berlin). The constitutively active GsαQ227L point mutant was a gift from Dr ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The IP-One ELISA assay kit from CisBio Bioassays ...
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bioRxiv - Neuroscience 2021Quote: ... and cell lysis buffer (IP-One assay kit, CisBio) was added (40 µl/well ...
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bioRxiv - Molecular Biology 2021Quote: The assays were performed using the EPIgeneous Methyltransferase kit (Cisbio). The reactions were performed by mixing the Clr4 constructs (30 nM ...
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bioRxiv - Neuroscience 2024Quote: The competitive cortisol assay (Cisbio HTRF® Cortisol Kit, 62CRTPEG) was performed following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.5 using the cAMP assay kit from Cisbio (Codolet, France) according to the supplier’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were collected every min for insulin assay using Insulin High Range Kits (Cat# 62IN1PEG, RRID:AB_2890910, Cisbio Assays, Bedford, MA) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: MK2 enzyme assay was optimized using HTRF KinEASE STKS1 kit from Cisbio. Briefly ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: MK2 enzyme assays were performed using HTRF KinEASE STKS1 kit from Cisbio HTRF technology ...
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bioRxiv - Cell Biology 2023Quote: ... Insulin content was measured using an insulin ultra-sensitive assay kit (Cisbio). Secreted insulin was normalized as percentage of total insulin content ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the stimulation buffer was removed and lysis buffer (IP-One assay kit, CisBio) was added (40 µl/well) ...
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bioRxiv - Cell Biology 2022Quote: HTRF assays were performed using the cAMP Gs dynamic kit (CisBio, Cat# 62AM4PEC) following the manufacturer’s protocols with slight modifications (Frenster et al ...
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bioRxiv - Physiology 2022Quote: ... Insulin was quantified using insulin HTRF (Insulin Ultrasensitive Assay kit 62IN2PEH; Cisbio, USA) and normalized to DNA content.
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bioRxiv - Biophysics 2021Quote: Inositol phosphate accumulation was measured using an IP-One Gq assay kit (Cisbio Bioassays). The WT and mutant CCKRs were cloned into pTT5 vector (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... Plasma insulin concentration was measured using the Insulin Mouse Serum Assay HTRF Kit (Cisbio) (WE-MET in Toulouse) ...
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bioRxiv - Neuroscience 2023Quote: ... The samples then entered a competitive cortisol assay (Cisbio HTRF® Cortisol Kit, 62CRTPEG) and the plate was read (TECAN ...
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bioRxiv - Cell Biology 2023Quote: ... Insulin was measured by using a Homogeneous Time Resolved Fluorescence (HTRF) insulin assay kit (Cisbio) in a PHERAstar reader (BMG Labtech) ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mM EGTA) and insulin content was measured by insulin ultra-sensitive assay kit (Cisbio). Within an experiment ...
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bioRxiv - Physiology 2020Quote: ... Insulin concentration (ng/ml) was measured using a HTRF Insulin Ultra-Sensitive Assay kit (Cisbio, France).
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bioRxiv - Biophysics 2022Quote: IP accumulation was measured using the IP-One HTRF® assay kit (Cisbio Bioassays, Condolet, France), following the official protocol from the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were collected every min for insulin measurement using Insulin High Range Kits (Cisbio Assays, Bedford, MA) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... IP1-d2 conjugate and the anti-IP1 cryptate Tb conjugate (IP-One Tb™ assay kit, CisBio) were diluted 1:40 in lysis buffer and 3 μl of each was added to each well ...
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bioRxiv - Cell Biology 2023Quote: ... Total insulin content was assessed using ultra-sensitive insulin homogeneous time-resolved fluorescence (HTRF) assay kits (Cisbio).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The IP1-d2 conjugate and the anti-IP1 cryptate Tb conjugate (IP-One Tb™ assay kit, CisBio) were diluted together 1:40 in lysis buffer and 3 μl of the antibody mix were added to each well ...
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bioRxiv - Cell Biology 2021Quote: MANF HTRF assay (CisBio) was used to determine circulating MANF concentration from 16μL of human plasma samples or cell culture media according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: IP1 accumulation assay: Inositol monophosphate accumulation was determined using the IP-One HTRF (Homogenous Time Resolved Fluorescence) kit (Cisbio Bioassays ...
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bioRxiv - Molecular Biology 2023Quote: IP1 accumulation assay was applied for detection of the downstream Gαq signal of MCHR2 using the IP-One HTRF kit (Cisbio). AD293 cells were cultured in DMEM/ high Glucose supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The accumulative cAMP assay was performed as previously described,45 using the CisBio cAMP-Gi kit (Cisbio Bioasays, Codolet, France). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... The cortisol ELISA assay was performed as per manufacturer’s instructions using the Cisbio HTRF cortisol ELISA kit (62CRTPEG, Cisbio, Perkin Elmer) and measurements were performed on Tecan Infinite M1000 Pro multi-plate reader.
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bioRxiv - Cell Biology 2022Quote: ... using the Taglite assay buffer (Cisbio) at a final volume of 20 μl per well ...
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bioRxiv - Biochemistry 2019Quote: ... Activity of hACs was determined in a volume of 10 μl using an Assist Plus pipetting robot (Integra Biosciences, Biebertal, Germany) and a cAMP assay kit from Cisbio (Codolet, France) according to the supplier’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were stored at −20°C until the insulin concentration was determined using an Insulin Ultra-Sensitive Homogeneous Time Resolved Fluorescence (HTRF) Assay kit (62IN2PEG, Cisbio, Codolet, France) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were stored at -20°C until the insulin concentration was determined using an Insulin Ultra-Sensitive HTRF Assay kit (62IN2PEG, Cisbio, Codolet, France) according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2023Quote: The inhibition of forskolin-stimulated cyclic AMP (cAMP) accumulation by MOR agonists was assessed using the Cisbio Homogeneous Time-Resolved Fluorescence (HTRF) cAMP Gi kit (Cisbio Assays, PerkinElmer). This kit uses a Förster Resonance Energy Transfer (FRET ...
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bioRxiv - Neuroscience 2022Quote: ... The Homogeneous Time Resolved Fluorescence assay (Total alpha Synuclein cellular assay, cat. 6FNSYPEG, Cisbio Bioassays, Codolet, France) was performed according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The homogeneous time-resolved fluorescence IP1 assay was used to measure intracellular IP1 accumulation according to the Cisbio IP-One Gq kit (Cisbio Bioassays, Perkin Elmer). Dose-response curves were fitted by Prism 8 (Graphpad) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were lysed at the end of the assay and analysed for total cAMP using HTRF (Cisbio cAMP Dynamic assay).
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bioRxiv - Immunology 2023Quote: ... supernatants obtained as described for the LDH release assays were subjected to human IL-1β Homogeneous Time Resolved Fluorescence (HTRF) assays (Cisbio) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: We used a TR-FRET cAMP assay (Cisbio) for estimating cAMP as these assays eliminate the background noise due to FRET-based estimation of signals ...