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Citations for Synaptic Systems :
1 - 50 of 580 citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 1 μl of Synaptotagmin-1 antibody (1 mg/ml, luminal domain - Synaptic System, 105 3FB) and 1 μl of FluoTag-X2 anti-mouse KLC (batch n ...
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bioRxiv - Neuroscience 2020Quote: ... were incubated with biotinylated antibodies against the luminal domain of VGAT (131 103BT, Synaptic Systems) in a modified Tyrode’s solution (4 mM KCl ...
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bioRxiv - Neuroscience 2020Quote: ... 2% Triton X-100 in PBS) and brain-specific homeobox/POU domain protein-3a (Brn3a; Synaptic Systems, Goettingen, Germany) diluted 1:1000 in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... VGAT (1:300, cytoplasmic domain, Synaptic Systems, 131013), GFP (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... labelling of hippocampal neurons with a cypHer-conjugated antibody directed against the luminal domain of VGAT (#131103CpH; Synaptic Systems) was performed by incubating primary neurons with anti-VGAT cypHer at 1:120 (from a 1 mg/ml stock ...
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bioRxiv - Neuroscience 2023Quote: ... RIM2 (anti-Rim2 PDZ domain, Synaptic Systems, Goettingen, Germany), CaV1.3(L-type Ca++CP ...
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bioRxiv - Neuroscience 2022Quote: ... the AβO-or vehicle-treated live neurons were incubated with an antibody recognizing the luminal domain of SynTag1 (1:500; mouse IgG1, clone 604.2, Cat# 105 311, Synaptic Systems) for 30 mins in culture medium at 37 °C in a 5% CO2 incubator ...
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bioRxiv - Neuroscience 2022Quote: ... IHC: 1:900) and rabbit polyclonal antibodies against the lumenal domain of VGAT (#131103, IC: 1:200) were from Synaptic Systems. Non-immune rabbit immunoglobulins were from Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Turnover of synaptic vesicles in mature cultures (DIV 15-25) was determined by live labeling with Atto647N-labeled mouse antibody against the luminal domain of synaptotagmin-1 (Synaptic Systems, cat ...
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bioRxiv - Biochemistry 2020Quote: Recycling synaptic vesicles were labelled using a mouse monoclonal antibody against the lumenal (intravesicular) domain of Synaptotagmin 1 (clone 604.2; Synaptic Systems, Göttingen, Germany) conjugated to either Sulfo-Cyanine 3 (#105 311C3 ...
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bioRxiv - Neuroscience 2023Quote: ... to analyze presynaptic excitatory punctae and the guinea pig anti-vesicular GABA transporter (VGAT) antibody (1:200, cytoplasmic domain, Synaptic Systems, RRID:AB_887871) to evaluate presynaptic inhibitory punctae ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were incubated for 5 min with 1:100 polyclonal rabbit antibodies directed against the lumenal domain of Syt1 conjugated to the fluorescent dye Oyster488 (#105 103C2; Synaptic Systems, Göttingen, Germany) in either plain Tyrode (stimulated condition ...
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bioRxiv - Neuroscience 2020Quote: ... neurons were incubated with 1:500 polyclonal rabbit antibodies directed against the lumenal domain of Syt1 conjugated to the fluorescent dye Oyster488 (#105 103C2; Synaptic Systems, Göttingen, Germany) during the TNR labeling step.
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bioRxiv - Neuroscience 2023Quote: ... spontaneously active synaptic boutons were labeled by incubating cells with CypHer5E-conjugated antibodies against the luminal domain of vGAT (1:500 from a 1 mg/ml stock; Synaptic Systems; Cat#131,103CpH) for 2 h in their respective conditioned culture media at 37 °C and 5 % CO2 prior to imaging.
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bioRxiv - Cell Biology 2024Quote: ... 10 HZ in the presence of 1:100 anti VGAT luminal domain polyclonal rabbit antibody conjugated to Alexa Fluor 488 (Synaptic Systems 131 103C2). Following washout ...
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bioRxiv - Neuroscience 2021Quote: ... cultured hippocampal neurons were incubated live for 10 min at 37 °C with a mouse monoclonal antibody raised against the N-terminal domain of GluA2 that also recognizes GluA1 and GluA3 (Synaptic Systems, 182411 Clone 248B7) and diluted to 1:100 in the culture medium ...
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bioRxiv - Microbiology 2022Quote: ... 40U/μL RNase Inhibitor) containing 2 μg anti-m6A rabbit polyclonal antibody (Synaptic Systems). The reaction was incubated with head-over-tail rotation at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... cells were treated with 10% NGS/90% antibody buffer containing primary antibodies against Bassoon (1:500; mouse; Enzo/Assay Designs) and Homer1 (1:500; rabbit; Synaptic Systems), at 4°C overnight in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... 40U/μL RNase Inhibitor) containing 2 μg anti-m6A rabbit polyclonal antibody (Synaptic Systems, #202003). The reaction was incubated with head-over-tail rotation at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: Tissue sections were washed three times in 0.1 M PBS and then transferred to a primary antibody solution containing 1:500 rabbit anti-c-Fos antibody (226003, Synaptic Systems, Gottingen, Germany), 4% normal goat serum ...
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bioRxiv - Neuroscience 2023Quote: ... then washed 3 times (10min each) and finally incubated overnight at 4°C with primary antibody solution containing anti-parvalbumin (Parvalbumin antibody, 195004, Synaptic System 1:1000) 1% BSA and 0.1% Triton in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies against the activity-regulated cytoskeleton-associated protein (ARC; guinea pig, Synaptic system), the glial fibrillary acidic protein (GFAP ...
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bioRxiv - Biochemistry 2020Quote: ... Intact SNAP-23 protein was detected with anti-SNAP23 polyclonal antibody (Synaptic Systems, 111203) diluted 1:1000 in 2% blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... and stained for the postsynaptic density protein Homer using a polyclonal antibody (Synaptic Systems) and an Alexa-555 secondary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... and then incubated in the blocking solution containing mouse anti-NeuN antibody (1:500, Synaptic Systems 266 004) and guinea pig anti-parvalbumin antibody (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... the sections were incubated overnight in washing buffer containing the rabbit anti-VAChT antibody (Synaptic Systems, #139103; 1:250) plus 2% normal goat serum ...
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bioRxiv - Immunology 2021Quote: ... proteins were transferred to nitrocellulose membranes and incubated with antibodies against VGLUT1 (dilution 1:1000, Synaptic Systems) at 4 °C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... co-cultures were incubated in GP anti-red fluorescent protein (RFP) antibody at 1:500 (Synaptic Systems) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 37°C overnight (16-18 hr) with rabbit anti-Ribeye A domain (1:200, Synaptic Systems Cat# 192 103, RRID:AB_2086775) and mouse anti-GluR2 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... with the primary antibody diluted in TBST solution containing 1% dry milk: custom-made rabbit anti-MDGA1 (Synaptic Systems #421002), rabbit anti-HA (Cell Signaling #3724 (C29F4) ...
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bioRxiv - Neuroscience 2024Quote: ... primary rabbit polyclonal antibodies against the c-Fos protein (226 003, Synaptic Systems, Goettingen, Germany, dilution 1:1000) and donkey secondary antibodies against rabbit conjugated with Alexa 488 (A-21206 ...
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bioRxiv - Neuroscience 2022Quote: ... coronal sections containing the dLGN were stained using a rabbit polyclonal vGlut2 antibody (1:500, Cedar Lane/Synaptic Systems 135403(SY), RRID:AB_887883 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4°C in blocking solution containing primary antibody: rabbit anti-c-Fos (1:3000; Synaptic Systems). The following day ...
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bioRxiv - Cell Biology 2020Quote: ... The following primary antibodies were used: Ras-related protein Rab-5A (RAB5A) at 1:500 (Synaptic Systems 108 011); early endosome antigen 1 (EEA1 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with primary antibodies diluted in PBS containing 0.3% BSA: anti-VGLUT1 (1:2000, Synaptic Systems, guinea pig polyclonal, RRID:AB_887878), anti-PSD-95 (1:50 ...
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bioRxiv - Neuroscience 2022Quote: ... with rabbit polyclonal antibody for rat TMEM119 that recognizes microglia-specific transmembrane proteins (Synaptic Systems GmbH, 1:1000, 400 203). For the secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5 % Triton X-100 % and 0.2 % BSA in PBS) containing the primary antibody (polyclonal rabbit anti-TH, 1:1000, Synaptic Systems Cat# 213 104) overnight at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the cryo-sections were incubated for 48 h at 4 °C (while shaking) with a solution containing mouse primary antibody against GluA1 (1:1000; Synaptic Systems, Cat# 182011) in 0.01 M PBS with 10 % NGS and 0.1 % Triton X-100.
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bioRxiv - Neuroscience 2023Quote: ... Tissue sections were then incubated in the blocking solution containing the guinea pig anti-c-Fos primary antibody (1:1000; Cat No. 226-308, Synaptic Systems; Gottingen, Germany) overnight at 4°C with constant agitation ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were rinsed in TBST and cut to be incubated for 1 h at room temperature in 0.5% non-fat dried milk in TBST containing the appropriate primary antibody anti-SP (rabbit polyclonal, Synaptic Systems, 163 002, 1:1000), anti-Beta-actin (mouse monoclonal ...
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bioRxiv - Neuroscience 2019Quote: ... We confirmed that expression of the fosGFP transgene correlated with the synthesis of the endogenous c-fos protein using a c-fos specific antibody (Synaptic Systems GmbH ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µg of RNA was incubated overnight at 4°C with protein A agarose beads treated with Rabbit IgG or anti-m6A polyclonal antibody (Synaptic Systems) in MeRIP buffer (Tris-HCl (pH 8.0),150 mM NaCl,0.1% NP-40 ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were used to immune stain overnight at 4°C the following proteins: Complexin (1:1000; Synaptic Systems; #122 002) and VGlut1 (1:4000 ...
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bioRxiv - Pathology 2021Quote: ... Neutralization was determined by monitoring the cleavage of VAMP-2 with a primary antibody specific for the intact form of the protein (Synaptic System 104 211) and an antibody specific for SNAP-25 (clone SMI81 Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... Fragmented RNA at 6 μg were added to 10 μl Slurry of PierceTM Protein A Agarose beads pre-incubated with 1 μg of an anti-m6A polyclonal antibody (Synaptic Systems, 202-003) by rocking at 4°C for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... 20 μg of protein lysate were loaded and analyzed by SDS-PAGE followed by immunoblotting: CaV2.1 α1 CT antibody (Synaptic Systems, #152 203, rabbit polyclonal) diluted 1:1000 (final concentration 2 μg/ml ...
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bioRxiv - Microbiology 2019Quote: ... and 10 μg of poly(A)+ RNA or virion gRNA was then used following the previously reported PA-m6A-seq protocol (10, 24) using either an m6A-specific (202111; Synaptic Systems) or m5C-specific (C15200081 ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.4) containing atto647N-labelled anti-GFP nanobodies (NB) (Synaptic Systems) at 3.19 pg μl-1 ...
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bioRxiv - Neuroscience 2020Quote: ... Brain sections were incubated with the indicated primary antibodies (Rabbit GABAAalpha3 antibody Synaptic Systems, 1:500; Mouse Gephyrin antibody Synaptic Systems, 1:500, Guinea pig VGAT antibody, Synaptic Systems, 1/500) for 48 hours at 4°C followed by secondary antibodies (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... guinea pig anti-neuronal nuclei protein (NeuN, also known as hexaribonucleotide-binding protein 3; 1:500, 266004, Synaptic Systems, RRID:AB_2619988); goat anti-nitric oxide synthase (NOS ...