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Citations for Worthington Biochemical :
1 - 50 of 50 citations for Strong Cation Exchange Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Ganglia were transferred to dissociation media (divalent cation-free HBSS with 2 mg/mL collagenase Type II (CLS2, Worthington, USA) for 60 min at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... purified by ion exchange chromatography and cleaved using papain (1:40 weight-to-weight ratio of papain (Worthington) to IgG ...
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bioRxiv - Microbiology 2022Quote: ... 18-24hrs post transfection, the media was replaced with viral growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
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bioRxiv - Microbiology 2020Quote: ... 1 hour after infection media was changed to serum-free media supplemented with 1µg/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Worthington-Biochemical). Passage experiments were performed by infecting cells at an MOI of 0.01 ...
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bioRxiv - Neuroscience 2023Quote: ... The L2-5 ganglia were dissected out and placed in cell culture media before being transferred to media containing neutral protease 3.125 mg/mL (Worthington, LS02104) and collagenase type 1 5 mg/mL (Worthington ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Developmental Biology 2021Quote: ... per mL of EHAA media and DNAse (Worthington). An equal volume of 0.1 M EDTA in Hank’s buffered saline solution without calcium or magnesium was added to the digested cells and incubated for 5 min at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... this media is similar to the MEM media described above but supplemented with Tosyl phenylalanyl chloromethyl ketone (TPCK) trypsin (Worthington Chemical Corporation) and with Bovine Albumin Fraction V (7.5% solution ...
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bioRxiv - Immunology 2021Quote: ... tissue was finely minced and placed into digestion media (RPMI basal media, 300 U/mL collagenase I [Worthington Biochemical Corporation], 1% BCS), shaken for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... per ml of EHAA media with DNAse (Worthington, Lakewood, NJ) at 37 degrees ...
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bioRxiv - Neuroscience 2023Quote: ... Cortices were dissociated in Neurobasal media containing papain (20U/mL, Worthington) and DNase I (100 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... Digestion media was prepared with 0.8 mg/mL Collagenase IV (Worthington) and 0.05 mg/mL DNase I (Roche ...
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bioRxiv - Immunology 2024Quote: ... per mL of EHAA media with DNAse 1(Worthington, Lakewood, NJ) at 37°C to isolate the stromal cell population for 1 hr at 37°C with pipetting every 15 min to physically agitate the digested tissues ...
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bioRxiv - Neuroscience 2023Quote: ... washed 5 minutes with Neurobasal media containing Ovomucoid (15 mg/mL, Worthington), and manually triturated in OptiMeM supplemented with D-Glucose (20mM) ...
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bioRxiv - Neuroscience 2023Quote: ... They were then dissociated in Neurobasal media containing papain (20U/mL, Worthington) and DNase I (100 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Digestion was performed in RPMI media containing 20 U/mL papain (Worthington Biochemical LS003119) and 0.005% DNAase I ...
Product cited:
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To...Cat# LS003119, 100 mg, $100.00 Ask -
bioRxiv - Immunology 2023Quote: ... and incubated with digestion buffer (RPMI media supplemented with (2mg/ml) collagenase IV (Worthington) and (187.5 μg/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... mechanically disrupted and digested in serum free media containing 2mg/ml collagenase type I (Worthington) and DNase I (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... digested in Click’s media containing 500 units/mL Collagenase II (Worthington, Columbus, OH, USA; LS004174) and Collagenase IV (Worthington ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... and the pellet was resuspended in 5 mL dissociation media containing 165U of Papain (Worthington) and DNAse type I (40 µg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... then digested with collagenase II (1100 U ml-1, Worthington in Hams F-10 media (Sigma)) for 90 min at 37°C with gentle rotation at 70 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated for 7 min with Neurobasal media containing Ovomucoïde (15 mg/mL, Worthington), and manually triturated 10 times with a 1000 ml pipette in OptiMeM supplemented with D-Glucose (20mM) ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Immunology 2023Quote: ... Tissues were mechanically cut and digested in complete media containing Collagenase type 2 (265 U/mL; Worthington) and DNase (250 U/mL ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Immunology 2021Quote: ... Cells were then DNAse treated with 4 ml dissagregation media (DMEM, 10% FBS, 100 μg/ml DNAse (Worthington)) for 20 min at 37 °C ...
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bioRxiv - Biophysics 2023Quote: ... The media was cut into small pieces and immersed in tubes containing both elastase (Elastase, Lyophilized ESL, Worthington) and collagenase (Collagenase ...
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bioRxiv - Microbiology 2020Quote: ... All four cell lines were infected with SARS-CoV-2/Australia/VIC01/2020 at a multiplicity of infection of 0.1 with infection inoculum composed of serum-free culture media and TPCK-treated trypsin (Worthington). The plates were incubated at 37 °C for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Dissected brains were chilled on ice and minced in digestion media containing with 0.2% collagenase type 3 (LS004182, Worthington) and 3 U/mL dispase (LS02104 ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... and then they were incubated in 20 mL of DMEM media containing 0.2 % collagenase type II (Worthington Biochemical Corporation) and 2.5 U/mL dispase (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Cells were then DNase treated with 4 ml disaggregation media (DMEM, 10% FBS, 100 μg/ml DNASE I (Worthington)) for 20 min at 37 °C ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The tissue was then titurated in a digestion media containing 10 U/ml elastase (#LS002280, Worthington Biochemical, Lakewood, NJ, USA) and incubated for 30 minutes at 37°C ...
Product cited:
Two times crystallized. Supplied as an aqueous suspension. This preparation must be diluted to...Cat# LS002280, 1 gm, $1065.00 Ask -
bioRxiv - Cell Biology 2019Quote: ... and plantaris were isolated from the mouse and placed directly into a 1 ml solution of F10 media with 4,000 U/ml of Collagenase I (Worthington Biochemicals). The tissue was digested for 15-40 minutes depending on muscle size in a 37°C water bath with agitation by inversion every 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Media was changed 18-24 hours post-transfection, with 2 mL of virus growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse hindlimb muscles were dissected and incubated in digestion media (2.5 U/mL Dispase II; Thermo and 0.2% w/v Collagenase Type II; Worthington in DMEM) on a shaking incubator at 37°C for 1.5 hours ...
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Nucleoprotein reassortment enhanced transmissibility of H3 1990.4.a clade influenza A virus in swinebioRxiv - Microbiology 2023Quote: ... the media was supplemented with 1 µg/ml of tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK) treated-trypsin (Worthington Biochemicals, Lakewood, NJ). Wild-type viruses were generated with their respective NP genes (wtNC/19-pdmNP and wtMN/18-trigNP) ...
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bioRxiv - Cancer Biology 2023Quote: Xenograft tumors were harvested and digested using 5mL digestion media (phenol red-free DMEM/F12 [Corning cat. 16-405-CV], 100 mg/mL gentamicin [Worthington, cat ...
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bioRxiv - Cell Biology 2024Quote: ... the EDL was isolated from the mouse and placed directly into a 1 ml solution of F10 media with 40,000 U/ml of Collagenase I (Worthington Biochemicals). The tissue was digested for 15-40 minutes depending on muscle size in a 37°C water bath with agitation by inversion every 10 minutes ...
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bioRxiv - Genomics 2020Quote: ... samples were cut into small pieces in artificial cerebrospinal fluid before being added into pre-warmed papain dissociation media (Worthington #LK003150). The dissociation solution was incubated for 45 minutes at 37°C ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2021Quote: ... Fresh tumor tissue post resection was collected in the HiberbateTM media and dissociated with an enzyme cocktail including 32 mg of Collagenase IV (Worthington #LS0042019); 10 mg of Deoxyribonuclease I (Worthington #LS002007 ...
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bioRxiv - Neuroscience 2019Quote: ... The digestion was quenched by adding dissociation media supplemented with 0.2% BSA and 10 mg/ml ovomucoid inhibitor (Worthington Cat. # LK003128), and samples were kept chilled for the rest of the dissociation procedure ...
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bioRxiv - Neuroscience 2019Quote: ... The digestion was quenched by adding dissociation media supplemented with 0.2% BSA and 10 mg/ml ovomucoid inhibitor (Worthington Cat. # LK003128), and samples were kept chilled for the rest of the dissociation procedure ...
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bioRxiv - Immunology 2020Quote: ... and both fragments were separately digested in a petri-dish at 37°C 5% CO2 in RF-10 media with 1.6 mg/ml type IV collagenase (Worthington, CLS-4) overnight (12 hours) ...
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bioRxiv - Genomics 2023Quote: ... Each sample was centrifuged at 1200 rpm x5 mins at 4°C and resuspended in 10 mL warmed digestive media (HBSS + 1% HEPES) with 1,000 U/mL collagenase type IV (Worthington, Cat: LS004188), and rotated for 30 min at 37 °C ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2024Quote: ... these tissues were finely minced and then digested at 37℃ in digestion buffer: RPMI 1640 (UCSF Media Production) with 1 mg/mL collagenase IV (Worthington Biochemical) and 0.1 mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... The obtained liver tissues were homogenized in 8 mL of RPMI1640 media containing 1.8 mg/ml Collagenase IV (WOR-CLS4-1, Worthington Biochemical Corporation, NJ, USA) and 112.3 µg/ml DNase I (11284932001 ...
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bioRxiv - Immunology 2024Quote: ... and then incubated in 5 mL digestion buffer (basal media, 10 µM Y-27632, 600 U/mL Collagenase IV (Worthington cat. No. LS004189), 0.1 mg/mL DNAse I (Sigma-Aldrich ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Evolutionary Biology 2022Quote: ... and 24 h post-transfection the media was supplemented with 1 μg/mL of tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals, Lakewood, NJ, USA). The rgH9N1 viruses were propagated on MDCK cells and harvested 72 hours post-inoculations ...
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bioRxiv - Immunology 2023Quote: ... Virus-infected cells were grown in MEM-BSA-TPCK media[20] that was prepared similar to the MEM media described above but supplemented with 1 µg/mL Tosyl phenylalanyl chloromethyl ketone (TPCK) trypsin (Worthington Chemical Corporation, Lakewood, NJ) and with Bovine Albumin Fraction V (7.5% solution ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were centrifuged and then resuspended in a pre-warmed DNase solution (L-WRN media supplemented to a final concentration of 200U/ml DNase [Worthington], 2.5 mM MgCl2, 500 μM CaCl2) and incubated for 5-7 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were centrifuged and then resuspended in a pre-warmed DNase solution (L-WRN media supplemented to a final concentration of 200U/ml DNase [Worthington], 2.5 mM MgCl2, 500 mM CaCl2) and incubated for 10 min at 37°C ...