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Citations for Worthington Biochemical :
1 - 50 of 304 citations for HBSS Solution since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... HBSS+H and Papain solution (45U, Worthington, Lakewood, NJ)) was incubated for 5min at 37 degrees ...
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bioRxiv - Molecular Biology 2020Quote: ... Retinas were transferred to 500μL digestion solution ((HBSS containing 2.5mg/mL papain (Worthington Biochemical, NJ, USA), 200U DNase I (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2024Quote: ... Each epithelium was then washed once with HBSS and resuspended in Earle’s Balanced Salt Solution (EBSS, Worthington, #LK003188) containing papain (20 U/mL ...
Product cited:
Earle's balanced salt solution (EBSS) as supplied in the Papain Dissociation System.Cat# LK003188, 1 vi, $61.00 Ask -
bioRxiv - Molecular Biology 2020Quote: ... Isolated hippocampi were washed with the HBSS solution then dissociated using the same buffer containing 0.25 percent trypsin (Worthington) for 8 mins at 37°C ...
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bioRxiv - Immunology 2019Quote: ... DRGs were further digested with 1 mL of collagenase type II/dispase solution (HBSS/10 mM HEPES/4 mg/mL collagenase type II (Worthington)/5 mg/mL dispase (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then incubated at 37°C for 3 minutes in a solution of HBSS/0.2% glucose with 10 mg/ml trypsin (Worthington Biochemical) and 1 mg/ml Deoxyribonuclease I (Worthington Biochemical) ...
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bioRxiv - Cell Biology 2023Quote: ... the hippocampi were washed twice with cold HBSS and individually incubated at 37°C for 20 min in Papain dissociation solution (45 U of papain (Worthington), 0.01% deoxyribonuclease (DNase) ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampi were dissected in ice-cold Hank’s balanced salts solution (HBSS) and kept on ice until digestion with 0.2 μm-filtered 10 U/mL papain (Worthington Biochemical Corporation) in HBSS in a 37°C water bath for 20 min ...
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bioRxiv - Physiology 2022Quote: ... after intracardiac perfusion of Phosphate Buffer Saline followed by a solution of HBSS containing type II collagenase at 2 mg/ml (Worthington, USA). Specific diets described below were introduced the day of the first dimerizer injection and maintained until sacrifice.
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bioRxiv - Neuroscience 2020Quote: Hippocampi and/or cortices from embryonic day 18 Sprague-Dawley rats of either sex was dissected in ice-cold Hanks Balanced Salt Solution (HBSS) and digested using a papain dissociation system (Worthington Biochemical Corporation). The appropriate density of neurons was plated on glass-bottom culture dishes (MatTek ...
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bioRxiv - Neuroscience 2020Quote: Hippocampi and cortices from embryonic day 18 Sprague-Dawley rats of either sex were dissected in ice-cold Hanks Balanced Salt Solution (HBSS) and digested using a papain dissociation system (Worthington Biochemical Corporation). The appropriate density of neurons was plated on glass-bottom culture dishes (MatTek ...
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bioRxiv - Physiology 2020Quote: ... HBSS containing 0.04% Collagenase Type 2 (Worthington), 50 mM HEPES (N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid) ...
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bioRxiv - Molecular Biology 2023Quote: Uterine pieces were washed with phenol red-free free HBSS(−) to remove epithelial cells and further incubated in phenol red-free free HBSS(−) with collagenase type III (#CLS3, Worthington, Lakewood, NJ) at 37.5°C for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... and dissociated in HBSS containing 0.25% papain (LS003127, Worthington Biochemical Corporation ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003127, 1 gm, $595.00 Ask -
bioRxiv - Cell Biology 2023Quote: ... and incubated in HBSS containing papain (Worthington; 14LU/mL) and DNase I (100Lμg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated in HBSS containing papain (Worthington; 14 U/mL) and DNase I (100 μg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in HBSS containing papain (Worthington; 14 U/ml) and DNase I (100 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in HBSS containing papain (10 U/mL, Worthington LK003178) and 5 μg/mL DNase I (Sigma-Aldrich D5025-150KU ...
Product cited:
A component of the Papain Dissociation System, for use in the tissue dissociation method of...Cat# LK003178, 5 vi, $98.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... DRG tissue was incubated in papain (45 U in HBSS+H, Worthington) for 20 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and digested with collagenase II (5 mg/ml in HBSS) (Worthington Biochemical, #LS004176) for 1 hour at 37 □ on shaker at speed of 200 rpm ...
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bioRxiv - Neuroscience 2020Quote: ... Papain digestion step consisted of 3 ml HBSS with ∼16 units/ml Papain (Worthington), 0.83 mM L-Cysteine ...
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bioRxiv - Physiology 2022Quote: Ganglia were enzymatically digested with 2mL HBSS containing 3mg/mL collagenase type II (Worthington), 7.5mg/mL dispase II and 0.25 mg/mL DNase I (30mn at 37°C) ...
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bioRxiv - Genomics 2022Quote: ... and were then incubated in 400 ul of HBSS containing 0.65 mg papain (Worthington Biochem) for 10 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... washed with cold HBSS and digested in 200 U/mL Collagenase II (Worthington Biochemical Corporation) and 0.1% Trypsin (GIBCO ...
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bioRxiv - Neuroscience 2022Quote: ... Resulting tissue was enzymatically disassociated in buffer containing HEPES-HBSS with DNase (Worthington Biochemical LS002007) and Liberase (Roche Applied Sciences 05401054001 ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002007, 100 mg, $350.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... digested in HBSS containing Collagenase type II (3 mg/ml; Worthington Biomedical Corp., Lakewood, NJ, USA) and Dispase (4 mg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... After adding 0.9 mL pre-warmed HBSS containing 10 mg/mL Collagenase Type 2 (Worthington, UK) samples were incubated for 25 min at 37°C rotating every 5 min and then washed with 2 mL pre-warmed DMEM/F12 supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... then digested in 1X HBSS medium containing 1 mg/mL DNase I (Worthington Biochem.com, cat#LS002145) and 2.5 mg/mL Collagenase Type I (Worthington Biochem.com ...
Product cited:
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bioRxiv - Developmental Biology 2022Quote: ... HBSS was removed and 2 ml of freshly made 1 mg/ml collagenase (Worthington Biochemical, LS004194) in HBSS was added to each tube ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... Papain solution (Worthington Biochemical ...
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bioRxiv - Physiology 2024Quote: ... Ears were cut into small pieces in HBSS containing 2mg/ml of collagenase I (Worthington Biochemical Corporation) and digested for 1h at 37℃ shaking water bath ...
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bioRxiv - Neuroscience 2024Quote: ... The DRG pieces were incubated in 5 ml HBSS containing 2 mg/mL Stemzyme (LS004106, Worthington Biochemical) and DNAse I (LS001239 ...
Product cited:
A specialized combination of Animal Free Clostridium histolyticum collagenase (Code: CLSAFB) and...Cat# LS004106, 50 mg, $110.00 Ask -
bioRxiv - Neuroscience 2024Quote: ... The tissue was rinsed with fresh HBSS and digested with 20 U/ml papain (#LS003126, Worthington Biochemical) (500 μl per one hippocampi Eppendorf ...
Product cited:
Supplied as a 2X crystalline suspension in 50 mM sodium acetate, pH 4.5. To insure full...Cat# LS003126, 100 mg, $88.00 Ask -
bioRxiv - Cell Biology 2021Quote: ... HBSS was discarded and stromal cells were released by digestion with 3 mg/ml Type I Collagenase (Worthington) in 1 ml HBSS for 10 min twice at 37°C with shaking at 110 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... Adrenal medullae were isolated and digested in CMF-HBSS containing collagenase type I (2.9 mg ml−1, Worthington), bovine serum albumin (BSA ...
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bioRxiv - Immunology 2024Quote: ... Remaining intestinal pieces were then incubated in HBSS containing 5% FBS and 150U/ml collagenase type 2 (Worthington) at 37°C with stirring for 45min to isolate LP cells ...
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bioRxiv - Biochemistry 2020Quote: ... DRGs were digested in 1 mL of Ca2+/Mg2+-free HBSS containing 20 U of papain (Worthington, Lakewood, NJ), 0.35 mg of L-cysteine and 1 μL of saturated NaHCO3 and incubated at 37°C for 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 mL of Ca2+/Mg2+-free HBSS containing 4 mg of collagenase type II and 1.25 mg of Dispase type II (Worthington) was added and incubated at 37°C for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... This was followed by a wash in ice-cold HBSS and subsequent incubation with papain (1U, Worthington Biochemicals, PAP2) for 5 min at 37 °C ...
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bioRxiv - Physiology 2024Quote: ... the minced tissue was incubated in 0.5% BSA/HBSS containing 0.2 mg/ml of collagenase type II (Worthington; LS004204) for 30 minutes ...
Product cited:
Collagenase, Type 2 (Code: CLS-2), which is filtered through a 0.22 micron membrane and...Cat# LS004204, 5x50 mg, $200.00 Ask -
bioRxiv - Cancer Biology 2021Quote: ... 100-200 mg of tumor bearing lung tissue was then thoroughly minced with Noyes scissors, prior to incubation with digestion buffer (HBSS supplemented with 5% HI FBS, 125 U/mL collagenase IV (Worthington) and DNase (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines were established from autochthonous lung tumors by microdissection and mechanical mincing in digestion buffer (HBSS with 1 M HEPES, 125 Units/mL Collagenase Type IV (Worthington) and 20 μg/mL DNAse (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Ganglia were transferred to dissociation media (divalent cation-free HBSS with 2 mg/mL collagenase Type II (CLS2, Worthington, USA) for 60 min at 37 °C ...
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bioRxiv - Physiology 2022Quote: Digestion of the left kidney was performed by a 20 minutes-incubation in HBSS containing type II collagenase at 0.5 mg/ml (Worthington, USA), followed by a 4 minutes-incubation in trypsin 0.04% at 37°C after removal of the capsule ...
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bioRxiv - Immunology 2022Quote: ... which consisted of HBSS containing Ca2+ and Mg2+ supplemented with 3.4 mg/ml collagenase type 1 (Worthington Biochemical Corporation LS004196) and 100 U/ml hyaluronidase (Sigma-Aldrich H3631) ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... Tumor pieces were chopped and incubated 5 min at 37°C in a HBSS-papain based lysis buffer (Worthington PAP) containing DNAse (0.01% ...
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bioRxiv - Cell Biology 2019Quote: ... Kidneys were then cut into small pieces and further dissociated by incubation for 30 min at 37°C in HBSS with 1 mg/ml collagenase (type 2, 255 U/mg, Worthington). To isolate glomeruli ...
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bioRxiv - Neuroscience 2022Quote: ... Pups were decapitated and hippocampi were dissected and incubated in dissociation solution (7 mL HBSS buffered saline, 150 μL 100mM CaCl2, 10 μL 1M NaOH, 10 μL 500mM EDTA, 200 units Papain [Worthington]) at 25°C for 1 h (rat ...
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bioRxiv - Cancer Biology 2021Quote: ... Liver was then digested by a pre-warmed digestion buffer consisting of HBSS and 5000 U collagenase I (Worthington Biochemical). The liver was then excised and cut on ice in GM and passed through a cell strainer ...
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bioRxiv - Immunology 2020Quote: Freshly resected tumor samples were mechanically dissociated and digested in HBSS medium with Collagenase IV (2.5 mg/mL) and DNAse I (0.2 mg/mL) (Worthington Biochemical Corporation) at 37°C for 30 minutes ...