Labshake search
Citations for Worthington Biochemical :
1 - 50 of 507 citations for 6 Octen 1 ol 3 7 dimethyl 1 formate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... muscle of 6-week old male C57 BL/7 mice was dissected out and treated with Collagenase Type 1 (Cat# LS4196 Worthington 400U/ml final concentration) for 1 hour at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: Both retinas of three Csf1rGFP mice were dissected at different timepoints (Day 1, 3, and 7) and incubated with papain (Worthington Biochemical, Freehold, NJ, USA) for 15 min as previously described (26) ...
-
bioRxiv - Neuroscience 2023Quote: ... and 1 unit/mL glucose-6-phosphate dehydrogenase (Worthington)] in a cuvette ...
-
bioRxiv - Developmental Biology 2024Quote: ... Tissue was enzymatically dissociated in 3mg/ml Collagenase Type 7 (Worthington, cat# CLS-6) in HBSS for 2-3hr at 37°C with frequent trituration using an Eppendorf ThermoMixer ...
-
bioRxiv - Developmental Biology 2024Quote: ... Tissue was enzymatically dissociated in 3mg/ml Collagenase Type 7 (Worthington, cat# CLS-6) in HBSS for 2-3hr at 37°C with frequent trituration using an Eppendorf ThermoMixer ...
-
bioRxiv - Molecular Biology 2022Quote: Calvarial osteoblasts were obtained from 3 to 5-day-old C57BL/6 mice by sequential enzyme digestion [1 mg/ml collagenase type II (Worthington Biochemical, Lakewood, NJ, USA) in Hanks’ balanced salt solution (Life Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Pellets were then suspended with 1 ml pre-warmed to 37°C A-mTE-D2 (7 mg ml-1 Dispase II, Worthington NPRO2 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were spun down and supernatant was removed before the addition of 1 ml Dispase-mUE (Dispase II [7 mg ml-1 DMEM Ham’s F12], Neutral Protease, Worthington NPRO2; and 10 μg μL-1 DNase). Cells were aspirated in the solution gently 40 times using a 1 ml pipette tip ...
-
bioRxiv - Molecular Biology 2019Quote: ... Hindlimbs from 6-7 pups/litter were digested with collagenase (Type II, Worthington-biochem, Lakewood, NJ) and pronase (EMD Millipore ...
-
bioRxiv - Neuroscience 2023Quote: ... and then treated with 1 mg/ml collagenase type 3 (Worthington Biochemical Corporation ...
-
bioRxiv - Cell Biology 2024Quote: ... After enzymatic digestion (DMEM GlutaMAX, 1% P/S, 3% papain [Worthington 3126] ...
-
bioRxiv - Neuroscience 2022Quote: ... Brain cortices from 6-7 animals were isolated and incubated with 50 units of papain (Worthington Biochemical, USA) in Earle’s balanced salt solution (EBSS ...
-
bioRxiv - Neuroscience 2023Quote: ... 165859) and collagenase type II (3 mg mL −1, cat. no. 4176, Worthington) for ∼40 min at 37 ⁰C under gentle agitation and centrifuged at 800 x g for 7 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... Retinas were washed 3 times for 3 min in L-15 solution and then placed in L-15 solution containing 7 U ml-1 papain (cat. no. 3126, Worthington Biochemical Corporation ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Digestion was performed by applying 7 mL 1× HBSS containing 600 U/mL Collagenase II (Worthington Biochemical, Lakewood, NJ, USA) and 60 U/mL DNase I (AppliChem ...
-
bioRxiv - Cell Biology 2019Quote: ... each consisting of calvaria from 6 pups were digested in 10 ml collagenase solution (1 mg/ml Collagenase type I and type II (1:3 ratio, Worthington Biochemical Corp., Lakewood, NJ), 1 mM CaCl2 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Homogenised tissue was added to a solution (total volume of 7 ml) composed by of 1 mg/ml collagenase IV (Worthington Biochemical Corp, USA), 0.02 mg/ml DNase 1 (New England Biolabs ...
-
bioRxiv - Immunology 2020Quote: ... and skin was minced finely with dissection scissors and mixed in a 6-well plate with 3 ml of digestion buffer consisting of 0.8 mg/ml Collagenase Type 4 (4188; Worthington), 0.02 mg/ml DNAse (DN25-1G ...
-
bioRxiv - Neuroscience 2021Quote: ... Prepontine regions from 5-6 dpf fish were dissected into Evans buffer and dissociated in 1 mg/ml neutral protease (Worthington) in Evans buffer for 30 min at room temperature with gentle shaking ...
-
bioRxiv - Immunology 2020Quote: ... the aortas were then cut into small pieces (1-2 mm) and incubated for 45 minutes at 37 °C in a type I collagenase (6 mg/ml, Worthington) and elastase (2.5 mg/ml ...
-
bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein mixtures were diluted in 1:6 ratio (v/v) using ultrapure water prior to digestion using sequencing grade trypsin (Worthington Biochemical) at 37°C for 16 hours ...
-
bioRxiv - Cell Biology 2020Quote: ... and the tissue underwent 7 × 7 min digestions in collagenase type II (Worthington Biochemical Corp, Lakewood, NJ) and sterile PBS supplemented with 20 mM glucose ...
-
bioRxiv - Developmental Biology 2022Quote: ... MM from E11.5 kidneys from CD-1 mice were dissociated with Collagenase Type 3 (Worthington Biochemical Corporation; Cat #LS004180) and DNase I (New England Biolabs ...
-
bioRxiv - Developmental Biology 2022Quote: ... and digested with gentle rocking for 1 h at 37°C in 3 mg/mL collagenase II (Worthington, LS004176) diluted in HBSS containing 5% fetal bovine serum (FBS) ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Microbiology 2022Quote: ... lung tissue was minced in 5 ml of 1 x PBS containing 3 mg/ml collagenase type IV (Worthington). Samples were incubated at 37°C for 45 min and washed with 1 x PBS three times ...
-
bioRxiv - Cancer Biology 2023Quote: ... The minced tumors were digested with 5ml to 10ml of 1 mg/ml Collagenase 3 (Worthington cat. no. LS004183) plus 0.5mg/ml Hyaluronidase (Sigma-Aldrich H3884 ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2021Quote: ... permeabilized cells were incubated with DNAse I recombinase (3-3000 U/ml in 50 mM Tris-HCL, pH 7.5, 1/ mg/ml BSA: Worthington, LK003170) prior to incubation with TUNEL mixture.
Product cited:
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered...Cat# LK003170, 1 vi, $28.00 Ask -
bioRxiv - Cell Biology 2020Quote: Organoid cultures within 2-3 weeks of primary plating were dissociated with 1 U/ml neutral protease (Worthington, Cat LS02100) and 100 KU of DNase I in organoid media ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Cell Biology 2022Quote: ... and the telencephalon was manually cut into pieces, followed by enzymatic digestion (DMEM GlutaMAX 1% P/S, 3% papain (Worthington 3126), 1.5% 10 mg ml−1 DNAse and 2.4% 12 mg ml−1 cystein ...
-
bioRxiv - Immunology 2020Quote: ... inflated with a 1.5 ml mixture of 1 ml low melting agarose (3% w/v) and 500 μl 4U/ml dispase (Worthington Biochem, LS02104), and incubated for 1 h at room temperature (RT) ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Biophysics 2022Quote: ... CaCl2 was added to a final concentration of 40 mM (21 µL of 1 M) followed by the addition of 75 U (3 µL of 25 U/µL) of micrococcal nuclease (MNase, Worthington Biochemical) and incubated at 37◦C for 5 min ...
-
bioRxiv - Developmental Biology 2024Quote: ... Zebrafish ventricles were fixed in 3% PFA for 5 min and incubated in PBS supplemented with 1 mg/mL collagenase type 4 (Worthington Biochemical) at 4°C overnight ...
-
bioRxiv - Neuroscience 2022Quote: ... Tissue from 2-3 hypothalamiwas pooled into 1-1.5 mL of HABG containing papain (20 u/mL, Worthington Biochemical, Lakewood, NJ, US). Tissue was broken into smaller pieces by pipetting 2-3 times with a P1000 pipet followed by a fire-polished glass pipette with a wide opening ...
-
bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
-
bioRxiv - Immunology 2020Quote: To prepare single-cell suspensions lungs were finely chopped with scissors in a 48 well plate and treated with 3 mg ml-1 collagenase III (Worthington, Lakewood, NJ), 5 μg ml-1 DNAse ...
-
bioRxiv - Neuroscience 2020Quote: ... incubated with 3% collagenase type 3 (Worthington), 3 U/ml dispase (Worthington ...
-
bioRxiv - Cell Biology 2021Quote: ... A single cell suspension of vascular stromal cells was isolated from minced iBAT (using scissors) digested for 90 min with collagenase IV (3 mg mL-1, Worthington, Lakewood NJ, USA) at 37 °C under gentle agitation16 ...
-
bioRxiv - Neuroscience 2023Quote: ... Dissociation medium was aspirated until 1 mL remained and an activated papain solution (1:1 papain [Worthington-Biochem] with 13.6 mM Cysteine-HCL ...
-
bioRxiv - Developmental Biology 2023Quote: ... in 1:1 DMEM:F12K supplemented with DNase I (Worthington Biochemical), 6.5mg/ml collagenase (Worthington Biochemical) ...
-
bioRxiv - Physiology 2020Quote: ... oocytes contained in 1-3 mL of ovary lobes extracted via survival surgery were isolated by enzymatic digestion with collagenase type II (Worthington Biochemical Corp., NJ, USA) under mechanical agitation ...
-
bioRxiv - Developmental Biology 2021Quote: ... 200 μL of 7 mg/mL trypsin (Cat.No. LS003708; Worthington) in L15 were added and incubated on an orbital rotator for 10 min or until a minimal amount of cell clumps was observed ...
Product cited:
Supplied as a chromatographically purified, diafiltered and lyophilized powder.Cat# LS003708, 100 mg, $52.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... 25200072)/0.1 mg/mL collagenase (Worthington, cat. no. CLS-7 LS005332)/1 mg/mL Dispase II (Gibco ...
-
bioRxiv - Neuroscience 2020Quote: ... prepared using 1:1 albumin-ovomucoid solution (10mg/ml of each) (Worthington, Cat# OI ...
-
bioRxiv - Genomics 2023Quote: ... for 1 hour (Patient #1) or 3.7mg/mL collagenase II (Worthington, LS004177) for 90 minutes followed by 6mg/mL dispase (Gibco ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... containing 1 mg/ml each of Collagenase Type 1 (Worthington Biochemical LS004216), Type 2 (Worthington Biochemical LS004204) ...
Product cited:
Collagenase, Type 1 (Code: CLS-1), which is filtered through a 0.22 micron membrane and...Cat# LS004216, 5x50 mg, $200.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... and 1% DNase (Worthington) at 37°C for 6 minutes at which point DMEM (Gibco ...
-
bioRxiv - Biochemistry 2024Quote: ... Lungs from 4 mice were minced into ∼1 mm pieces using curved scissors and digested with 1 mg/ml collagenase type 1 (Worthington, cat# LS004194) and 1X dispase (Corning ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Genetics 2020Quote: ... 1 mg ml-1 soybean trypsin inhibitor (#LS003570, all purchased from Worthington Biochemical Corporation), and 1% penicillin/streptomycin in HBSS for 10 min at 37°C with gentle shaking ...
Product cited:
Chromatographically purified as described by Frattali and Steiner, Biochem., 7, 521 (1968). A...Cat# LS003570, 100 mg, $74.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... papain (1 mg/ml, Worthington), ethylenediaminetetraacetic acid (EDTA ...