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Citations for Worthington Biochemical :
301 - 350 of 379 citations for 6 Chloro 7H pyrrolo 2 3 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... the lungs were cut in very small pieces and digested in 1.5 ml of Collagenase Type 2 (Worthington, 44N15307B) for one hour at 37°C and 500 rpm ...
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bioRxiv - Cancer Biology 2022Quote: ... Fresh schwannomas were minced with sterile Bard-Parker #10 surgical scalpels (Aspen Surgical) and incubated in 0.4% Collagenase Type 2 (Worthington) in pre-oxygenated Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... muscles were chopped in PBS on ice and digested using 800 U/mL collagenase type 2 (Worthington, Cat# LS004177) in F10 medium (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... as well as on day 1 and 2 after RFA were dissociated using 20 U/ml papain43 (Worthington Biochemical, USA), filtered through a 70 μm filter and resuspended in 3% BSA in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... The dissected hypothalamuses were further divided into 2-4 pieces before cell dissociation for single cell isolation using Papain Dissociation System (cat no. LK003150, Worthington Biochemical Corporation ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Developmental Biology 2019Quote: ... The hearts were rapidly excised and the aorta was cannulated onto a Langendorff apparatus and perfused with 2 mg/mL collagenase II (Worthington). Digestion was stopped after the hearts lost partial integrity ...
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bioRxiv - Developmental Biology 2019Quote: ... and then the freshly isolated heart was cannulated onto a needle and perfused with 2 mg/mL collagenase II (Worthington) until the heart loses integrity ...
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bioRxiv - Cell Biology 2019Quote: ... A single cell suspension was made by digesting tissue for 30 minutes at 37°C in Collagenase type 2 solution (Worthington Biochemical ...
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bioRxiv - Immunology 2019Quote: ... The Epidermis was further minced and then digested for an additional 30 min with 2 mg/mL Collagenase IV (Worthington) with 0.05 mg/mL DNAse I ...
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bioRxiv - Immunology 2021Quote: ... tissue disaggregation was performed by finely chopping liver samples (~1-2 g) in 10 ml digestion solution containing 1 mg/ml Collagenase IV (Worthington) and 20 μg/ml DNAse1 (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... coli was removed by mixing TNKS1 construct with 2 µM snake venom phosphodiesterase I from Crotalus adamanteus (Worthington Biochemical Corporation).
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bioRxiv - Microbiology 2019Quote: ... All viral growth was carried out in MEM with 1 μg/ml trypsin treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK-treated trypsin; Worthington), 1 mM HEPES ...
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bioRxiv - Immunology 2022Quote: Lung tissue was minced and digested in a RPMI 1640 solution with Type 2 collagenase (0.5 mg/mL) (Worthington Biochemical) and 5 units/mL DNAse (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Ganglia were transferred to dissociation media (divalent cation-free HBSS with 2 mg/mL collagenase Type II (CLS2, Worthington, USA) for 60 min at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Surgical samples were minced then digested for 1 hour at 37 °C with 2 mg/mL collagenase I (Worthington, #LS004197) and 200 U/mL deoxyribonuclease (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004197, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2022Quote: ... The tumor-bearing left lungs were mechanically dissociated using a razor blade and incubated for 30 minutes at 37°C with 3.2mg/mL Collagenase Type 2 (Worthington, 43C14117B), 0.75 mg/ml Elastase (Worthington ...
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bioRxiv - Cell Biology 2019Quote: ... Kidneys were then cut into small pieces and further dissociated by incubation for 30 min at 37°C in HBSS with 1 mg/ml collagenase (type 2, 255 U/mg, Worthington). To isolate glomeruli ...
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bioRxiv - Physiology 2020Quote: ... minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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bioRxiv - Immunology 2020Quote: ... Lungs were instilled with either 1 mL of 10% formalin for histology studies or 1 mL of digestion buffer (90%DMEM 10% PBS+Calcium with 180 U/mL Type 2 Collagenase (Worthington) and 15 μg/mL DNase (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... skins from early postnatal pups (P5-P7) were isolated and enzymatically dissociated using 2 mg/mL collagenase type I (Worthington Biochemical Corp ...
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bioRxiv - Cell Biology 2019Quote: Ventricular cardiomyocytes isolated from rats or mice at postnatal day 2 or day 1 respectively using the Neonatal Cardiomyocyte isolation System (Worthington) were cultured as previously reported.22 To induce the deletion of Psmc1 ...
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bioRxiv - Microbiology 2020Quote: ... Media was changed 18-24 hours post-transfection, with 2 mL of virus growth media (MEM, 1 mM HEPES, 1 μg/mL TPCK trypsin (Worthington Biochemical Corporation ...
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bioRxiv - Biochemistry 2022Quote: ... Each 25 μL piece of vitreous was placed in an 8 mm diameter coverslip-bottom chamber with 200 μL of either 2 mg/mL bacterial collagenase type II (Worthington) or phosphate-buffered saline ...
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bioRxiv - Neuroscience 2022Quote: ... and the sensory neurons were dissociated by mechanical agitation after incubation for 2 h with collagenase type III (CLS3, Worthington) at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were digested with 1:50 (m/m) lysyl endopeptidase C (Fujifilm) at 42 °C for 2 hours and 1:50 (m/m) trypsin (Worthington) at 37 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: Cerebral cortices from P4-6 CD1 mouse pups were dissected in ice-cold HBSS without Mg2+ and Ca2+ and triturated with 2 ml of digestion buffer containing 20 U/ml papain (Worthington) and 100 U/ml DNase I (Worthington ...
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bioRxiv - Bioengineering 2022Quote: ... In vitro degradation of lignin composites was determined by submerging lignin composites in the solution of collagenase type II (0.5 U/mL, cat# CLS-2, Worthington Biochemical) with 1 mM CaCl2 in serum-free culture medium ...
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bioRxiv - Immunology 2023Quote: ... whole lung was minced with a sharp razor blade and digested with collagenase type IV 2 mg/ml (Worthington, LS004189) and deoxyribonuclease II 4 μg/ml (Worthington ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2023Quote: ... The right lobes of the lungs were collected in 5 mL of digestion buffer (90% DMEM and 10% PBS and calcium with type 2 collagenase (180 U/ml) (Worthington) and DNase (15μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... the brain tissues were washed with DM 1∼2 times and incubated with 13mL of DM containing papain (Worthington, LK003176) in 37°C water bath for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... D6/2-rotavirus was propagated in MA104 cells in serum-free DMEM supplemented with 1 µg/mL Worthington’s Trypsin (Worthington Biochemical). Harvested cells were then subjected to three freeze/thaw cycles ...
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bioRxiv - Immunology 2024Quote: ... The cardiac tissues were minced and subjected to repetitive rounds of enzymatic digestion with collagenase type 2 (Worthington Biochemical Corp.) until complete dissociation ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated at 37°C in serum-free cell culture medium (IMDM) supplemented with 1 µg/ml L-1-tosylamide-2-phenylethyl chloromethyl ketone (TPCK) trypsin (Worthington-Biochemical). Cell supernatants were harvested at indicated time points post-infection ...
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bioRxiv - Neuroscience 2020Quote: ... muscles were cut into 5 mm2 pieces with forceps and digested in 2 mg/mL collagenase II (Worthington Chemicals, Lakewood, NJ) for 1h at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... A 5 to 7-day-old protonemal tissue culture was scraped from solid plates and added to a 466 mM mannitol solution with 2% Driselase and Cellulase (21 unit/mL, Worthington CEL). The mixture was gently agitated for 1 h at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was digested for 30 min using 2 mL of HEPES-buffered HBSS containing 20 U/mL of papain (Worthington Biochem.), 1 mM EDTA (PanReac AppliCham ...
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bioRxiv - Physiology 2022Quote: ... after intracardiac perfusion of Phosphate Buffer Saline followed by a solution of HBSS containing type II collagenase at 2 mg/ml (Worthington, USA). Specific diets described below were introduced the day of the first dimerizer injection and maintained until sacrifice.
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bioRxiv - Immunology 2019Quote: ... and the GL261 tumors were excised and dissociated in Collagenase IV Cocktail (3.2 mg/mL Collagenase Type 4, 1.0 mg/mL deoxyribonuclease I, 2 mg/mL Soybean Trypsin Inhibitor [Worthington Biochemical, Lakewood, NJ]) for two 15 min intervals with gentle mixing at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... hindlimb muscles from 2-4-month-old mice were minced and digested in DMEM containing collagenase type II (0.2%; Worthington Biochemical Corporation) and dispase II (2.5 U/mL ...
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bioRxiv - Cell Biology 2020Quote: ... muscles were cut into 5 mm2 pieces with forceps and digested in 2 mg/mL collagenase II (Worthington Chemicals, Lakewood, NJ) for 1h at 37°C ...
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bioRxiv - Immunology 2021Quote: CHO cells stably expressing wild-type SARS-CoV-2 S were resuspended in wash buffer (PBS 1 % BSA, 2 mM EDTA) and treated with 10 µg/mL TPCK-trypsin (Worthington Biochem) for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: Unlabeled and Alexa Fluor 488 (AF488)-labeled T3DCD or T1L virions (2×1012 particles/ml) were digested with 200 μg/ml Nα-p-tosyl-L-lysine chloromethyl ketone-treated chymotrypsin (Worthington Biochemical) in a total volume of 100 μl for 20 min at 32°C [34] ...
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bioRxiv - Neuroscience 2020Quote: ... and the supernatant aspirated before the tissue was incubated in a collagenase solution containing 2 mg/mL collagenase type II (CLS2; Worthington Biochemical) in 5 mL 1X HBSS in a 37°C rocking water bath for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... with 2 mL pre-warmed digestion enzyme in ACSF solution (Papain, 25 unit/mL and DNase I, 55 unit/mL, Worthington Biochemical). For spinal dorsal horn neuronal atlas construction ...
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bioRxiv - Cell Biology 2024Quote: ... whole hearts from 28 wk-old ♂ WT and R14Δ/+ mice were cannulated and perfused with modified Krebs buffer containing 2 mg/ml collagenase (Worthington) for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclei from 2 X 108 Hela or 293T cells were digested with 20 U of micrococcal nuclease (Worthington Biochemical Corporation, LS004797) for 45 minutes ...
Product cited:
Chromatographically purified to be essentially homogeneous chromatographically and...Cat# LS004797, 15 ku, $94.00 Ask -
bioRxiv - Biochemistry 2024Quote: ... epidydimal fat from 4-month-old male DIO C57BL/6J mice was dissected out and dissociated using 2 mg/ml Collagenase B (Worthington, CLSAFB) and 1 mg/ml soybean trypsin inhibitor (Worthington ...
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bioRxiv - Genetics 2023Quote: ... and perfused with a type 2 collagenase digestion buffer (2.4 mg/ml concentration) at a flow rate of 4 ml/min (Worthington Cat# LS004176). The large blood vessels and the atria were excised from the ventricles and minced in a stop buffer containing 10% calf serum ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Molecular Biology 2023Quote: ... WAT depots from Scd1fl/fl mice were minced with scissors and digested for 1 h at 37°C in 2 mg/ml collagenase type I (Worthington Biochemical) in Krebs-Ringer-HEPES (KRH ...
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ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the HeartbioRxiv - Molecular Biology 2024Quote: ... and hearts were perfused retrogradely for 15-18 min at 37°C with HEPES-buffer containing 100 mg/mL collagenase type 2 (Cat#9001-12-1, Worthington, USA) and digested ...