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Citations for AAT Bioquest :
1 - 50 of 140 citations for 7 tert butoxycarbonyl 5 6 7 8 tetrahydro 1 2 4 triazolo 4 3 a pyrazine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and 7-diethylaminocoumarin-3-carboxylic acid was purchased from AAT Bioquest (Sunnyvale, CA). Dibenzocyclooctyne acid was purchased from Click Chemistry Tools (Scottsdale ...
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bioRxiv - Bioengineering 2022Quote: ... after which 4 μL of 1 mg/mL 7-AAD (AAT Bioquest, Sunnyvale, CA, USA) was added ...
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bioRxiv - Cell Biology 2024Quote: Caspase-3 activity as an important initiator of apoptosis was assessed by the AmpliteTM Fluorimetric Caspase 3/7 Assay Kit (AAT Bioquest) and performed according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: Apoptosis was measured using Cell MeterTM Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in GlomaxTM luminometer at Ex/Em= 490/525 nm.
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bioRxiv - Cell Biology 2019Quote: ... Apoptosis was measured using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in Glomax™ luminometer at Ex/Em = 490/525 nm.
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bioRxiv - Physiology 2020Quote: ... Apoptosis was measured using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in Glomax™ luminometer at Ex/Em = 490/525 nm.
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bioRxiv - Cell Biology 2019Quote: ... Apoptosis was measured using Cell Meter™ No-Wash Live Cell Caspase 3/7 Activity Assay Kits (#20250, AAT Bioquest). Caspase 3/7 activity data were normalized to total number of live cells (determined by CyQUANT kit ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were further stained with 7-AAD or 7-aminoactinomycine (AAT Bioquest, 17501). Data were acquired on a BD AccuriTM C6 flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Stained samples were washed and resuspended in 500 μL of 1x PBS + 2% FBS (flow buffer) before 5 μg/mL of 7-AAD (AAT Bioquest) was added ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Cell Biology 2022Quote: ... cells were loaded with 4 μM Fluo-8-AM (AAT Bioquest) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... followed by 0.5 μg/mL 7-AAD (AAT Bioquest). Single-stain and FMO (fluorescence-minus-one ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Attached cells were loaded with the Ca2+ dye Fluo-8 at room temperature (23 – 25 °C) by adding 4 μM Fluo-8 AM (AAT Bioquest) and 0.02% Pluronic F-127 (SiChem ...
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bioRxiv - Bioengineering 2021Quote: ... cells were incubated with 3 μL of 10 mM Fluo-8 AM solution (21082, AAT Bioquest, US), 1.2 μL of 10% (w/v) ...
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bioRxiv - Neuroscience 2023Quote: ... Fluo-8 (AAT Bioquest; final 5 μM), Probenecid (final 1 mM) ...
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bioRxiv - Neuroscience 2023Quote: ... and 2% pluronic acid (AAT Bioquest) in ASW was injected into one of the optic lobes using a glass micropipette needle (Harvard Apparatus Cat ...
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bioRxiv - Microbiology 2019Quote: ... 32 using the AmpliteTM Fluorimetric sn-Glycerol-3-Phosphate (Gro-3-P) Assay Kit (AAT Bioquest). GAC was released from cell wall by sequential digestion with mutanolysin hydrolase and PlyC amidase ...
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bioRxiv - Developmental Biology 2022Quote: ... TO-PRO-3 (AAT Bioquest 17572) was used to distinguish the live GFP+ cells and added at 1:1000 concentration (in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The lipophilic dye FM 4-64 (1 μg/mL) (AAT Bioquest) was used to stain membranes ...
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bioRxiv - Microbiology 2020Quote: ... samples were incubated for 5 min with 1.2 µg ml-1 of the membrane dye MM 4-64 (AAT Bioquest Sunnyvale, CA) and 0.5 µg ml-1 of 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2023Quote: ... and fluorescein-PE [N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine] was obtained from AAT Bioquest Inc.
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bioRxiv - Cell Biology 2022Quote: ... Fluo8-AM (4 μM, AAT Bioquest) was loaded in physiological buffer with sulfinpyrazone ...
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bioRxiv - Cell Biology 2019Quote: ... stained with 2 μM Fluo-8 AM (AAT Bioquest) for 30 min ...
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bioRxiv - Physiology 2023Quote: ... 3 μM Fluo-8H AM (AAT Bioquest, Pleasanton, CA) for 15 min at 37 °C and seeded on 5% 3-aminopropyltriethoxysilane-coated coverslip for 15 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... primary microglia of indicated groups were cultured in heat-inactivated DMEM medium containing 5 μM Fluo-4 AM (AAT Bioquest), 0.4 % F-127 (Beyotime ...
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bioRxiv - Biophysics 2022Quote: ... #14175103)] followed by incubation for 1 hour at 37°C and 5% CO2 in 1800 µl of imaging buffer containing 3 mM Cal-520-AM (AAT Bioquest; #21130) Cal-520-AM-loaded cells were removed from the CO2 incubator and equilibrated at room temperature for 5 minutes prior to IP3 stimulation by the addition of 200 µl of 1 mM carbachol (Alfa Aesar ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were treated with Fluo-8 AM (5 µM, AAT Bioquest) for 45 min and then washed twice with PBS ...
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bioRxiv - Biophysics 2020Quote: ... culture medium were discarded and cells were incubated with 50 μl per well of 4 μM Rhod-2/AM (AAT Bioquest) in TARODES’s buffer (10 mM HEPES ...
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bioRxiv - Neuroscience 2021Quote: Neurons were incubated in a calcium indicator dye solution consisting of 5 μM Rhod-4 AM (AAT Bioquest #21122; Sunnyvale, CA, USA), 1X PowerLoad (Thermo Fisher Scientific #P10020) ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were loaded with Cal590 (3 μg/ml, AAT Bioquest) for 50 minutes and solutions containing OT with various concentrations (0 ...
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bioRxiv - Physiology 2022Quote: To analyze voltage changes in the SAN preparation we loaded it by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator Di-4-ANEPPS (2 μmol/L; AAT Bioquest, Sunnyvale, California). This immersion was performed at room temperature (20-22°C ...
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bioRxiv - Immunology 2023Quote: Recombinant TRAF6(50-350) and other variants were labeled with Cyanine 3 (AAT Bioquest), UBE2N ...
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bioRxiv - Biophysics 2022Quote: ... DiYO-1 (CAS 143413-85-8) was purchased from AAT Bioquest. X-31 influenza virus (A/Aichi/68 ...
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bioRxiv - Biophysics 2022Quote: ... DiYO-1 (CAS 143413-85-8) was purchased from AAT Bioquest. Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanloamine (TR-DHPE ...
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bioRxiv - Biochemistry 2021Quote: ... HspB8 and variants thereof were labelled with Cyanine-3-monosuccinimidyl ester (AAT bioquest, ABD-141) at equimolar ratio in HspB8 buffer and excess dye was removed by dialysis against HspB8 buffer with 1 mM DTT ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with CalBryte-630AM (AAT Bioquest 20720, 3 µg/mL in 0.3% DMSO) calcium indicator for 30 minutes in the dark ...
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Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: ... Myoblasts were loaded with 4 μM Cal-520-AM (AAT Bioquest, CA, USA) for 45 minutes then washed in recording medium containing (mM ...
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bioRxiv - Neuroscience 2022Quote: ... Sample concentrations were generated using a 4 Parameter Logistic Curve Calculator (AAT Bioquest, “Quest Graph Four Parameter Logistic (4PL ...
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bioRxiv - Neuroscience 2021Quote: ... loading with the calcium indicator CalBryte-630AM (AAT Bioquest 20720, 3 μg/mL in 0.3% DMSO) followed the same protocol.
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bioRxiv - Molecular Biology 2023Quote: Dispersed islet cells were laded with 4 μm Calbryte 520 AM (AAT Bioquest 20650) in loading solution (2 mM Glucose in KRBH buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Texas Red-DHPE (1,2-dihexadecanoly-snglvero-3-phosphoethanolamine-[N-(Texas Red sulfonyl)]) was purchased from AAT Bioquest. PEG-biotin (Biotin-PEG SVA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Isolated single FDB cells were incubated with 4 µM Cal520-AM (AAT Bioquest, CA, USA) or fura-2-AM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... we loaded cytotoxic T cells for 30 minutes with 4 μM Cal-520 (AAT Bioquest), and transferred 2 x 104 cells per well into flat-bottom 96 well plates coated with a CD3 antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... T-tubules from intact hearts were stained with MM 4-64 (2.5 μM, AAT BioQuest) in Ca2+ free Tyrode solution via Langendorff perfusion at room temperature for 30 min as described previously (53 ...
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bioRxiv - Immunology 2024Quote: ... The standard curve was created with an online 4-PL curve calculator from AAT Bioquest (https://www.aatbio.com/tools/four-parameter-logistic-4pl-curve-regression-online-calculator) ...
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bioRxiv - Neuroscience 2023Quote: iDANoids were incubated for 30 min at 37 °C with 3 µM Calbryte 520 AM calcium indicator (AAT Bioquest) in maturation medium containing 0.02% Pluronic F-127 (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Assay plates for detection of intracellular calcium were washed twice in phosphate buffered saline (PBS) before incubated (37 °C for at least 30 min) with DMEM containing 2 µM of FluoAM-8 dye (AAT Bioquest) per well ...
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bioRxiv - Cancer Biology 2020Quote: Cell samples were incubated with the calcium indicator dye (4 mM of Cal-520 AM, AAT Bioquest) for 1 hr ...
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bioRxiv - Genomics 2024Quote: Fluorescent probes were generated using the Nick translation DNA labeling system 2.0 (Enzo) with XFD 488-dUTP or Cyanine-3-dUTP (AAT Bioquest). Unincorporated nucleotides were removed using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with the Ca2+ indicator dye Fluo-4 AM (10 μM, AAT Bioquest, Sunnyvale, CA, USA) for another 30-45 min at 37°C ...