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Citations for AAT Bioquest :
1 - 50 of 50 citations for 7 3 Chloropropoxy 4 chloroquinazoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and 7-diethylaminocoumarin-3-carboxylic acid was purchased from AAT Bioquest (Sunnyvale, CA). Dibenzocyclooctyne acid was purchased from Click Chemistry Tools (Scottsdale ...
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bioRxiv - Cell Biology 2024Quote: Caspase-3 activity as an important initiator of apoptosis was assessed by the AmpliteTM Fluorimetric Caspase 3/7 Assay Kit (AAT Bioquest) and performed according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2022Quote: ... after which 4 μL of 1 mg/mL 7-AAD (AAT Bioquest, Sunnyvale, CA, USA) was added ...
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bioRxiv - Cell Biology 2023Quote: Apoptosis was measured using Cell MeterTM Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in GlomaxTM luminometer at Ex/Em= 490/525 nm.
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bioRxiv - Cell Biology 2019Quote: ... Apoptosis was measured using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in Glomax™ luminometer at Ex/Em = 490/525 nm.
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bioRxiv - Physiology 2020Quote: ... Apoptosis was measured using Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit (AAT Bioquest, ABD-22796). Fluorescence intensity was analysed in Glomax™ luminometer at Ex/Em = 490/525 nm.
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bioRxiv - Cell Biology 2019Quote: ... Apoptosis was measured using Cell Meter™ No-Wash Live Cell Caspase 3/7 Activity Assay Kits (#20250, AAT Bioquest). Caspase 3/7 activity data were normalized to total number of live cells (determined by CyQUANT kit ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were further stained with 7-AAD or 7-aminoactinomycine (AAT Bioquest, 17501). Data were acquired on a BD AccuriTM C6 flow cytometer (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... followed by 0.5 μg/mL 7-AAD (AAT Bioquest). Single-stain and FMO (fluorescence-minus-one ...
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bioRxiv - Microbiology 2019Quote: ... 32 using the AmpliteTM Fluorimetric sn-Glycerol-3-Phosphate (Gro-3-P) Assay Kit (AAT Bioquest). GAC was released from cell wall by sequential digestion with mutanolysin hydrolase and PlyC amidase ...
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bioRxiv - Developmental Biology 2022Quote: ... TO-PRO-3 (AAT Bioquest 17572) was used to distinguish the live GFP+ cells and added at 1:1000 concentration (in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Fluo8-AM (4 μM, AAT Bioquest) was loaded in physiological buffer with sulfinpyrazone ...
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bioRxiv - Physiology 2023Quote: ... 3 μM Fluo-8H AM (AAT Bioquest, Pleasanton, CA) for 15 min at 37 °C and seeded on 5% 3-aminopropyltriethoxysilane-coated coverslip for 15 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... Stained samples were washed and resuspended in 500 μL of 1x PBS + 2% FBS (flow buffer) before 5 μg/mL of 7-AAD (AAT Bioquest) was added ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were loaded with Cal590 (3 μg/ml, AAT Bioquest) for 50 minutes and solutions containing OT with various concentrations (0 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were loaded with 4 μM Fluo-8-AM (AAT Bioquest) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The lipophilic dye FM 4-64 (1 μg/mL) (AAT Bioquest) was used to stain membranes ...
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bioRxiv - Immunology 2023Quote: Recombinant TRAF6(50-350) and other variants were labeled with Cyanine 3 (AAT Bioquest), UBE2N ...
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bioRxiv - Biochemistry 2021Quote: ... HspB8 and variants thereof were labelled with Cyanine-3-monosuccinimidyl ester (AAT bioquest, ABD-141) at equimolar ratio in HspB8 buffer and excess dye was removed by dialysis against HspB8 buffer with 1 mM DTT ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with CalBryte-630AM (AAT Bioquest 20720, 3 µg/mL in 0.3% DMSO) calcium indicator for 30 minutes in the dark ...
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Biallelic loss-of-function OBSCN variants predispose individuals to severe, recurrent rhabdomyolysisbioRxiv - Genetics 2021Quote: ... Myoblasts were loaded with 4 μM Cal-520-AM (AAT Bioquest, CA, USA) for 45 minutes then washed in recording medium containing (mM ...
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bioRxiv - Neuroscience 2022Quote: ... Sample concentrations were generated using a 4 Parameter Logistic Curve Calculator (AAT Bioquest, “Quest Graph Four Parameter Logistic (4PL ...
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bioRxiv - Neuroscience 2021Quote: ... loading with the calcium indicator CalBryte-630AM (AAT Bioquest 20720, 3 μg/mL in 0.3% DMSO) followed the same protocol.
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bioRxiv - Molecular Biology 2023Quote: Dispersed islet cells were laded with 4 μm Calbryte 520 AM (AAT Bioquest 20650) in loading solution (2 mM Glucose in KRBH buffer ...
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bioRxiv - Bioengineering 2021Quote: ... cells were incubated with 3 μL of 10 mM Fluo-8 AM solution (21082, AAT Bioquest, US), 1.2 μL of 10% (w/v) ...
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bioRxiv - Bioengineering 2023Quote: ... Texas Red-DHPE (1,2-dihexadecanoly-snglvero-3-phosphoethanolamine-[N-(Texas Red sulfonyl)]) was purchased from AAT Bioquest. PEG-biotin (Biotin-PEG SVA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Isolated single FDB cells were incubated with 4 µM Cal520-AM (AAT Bioquest, CA, USA) or fura-2-AM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... we loaded cytotoxic T cells for 30 minutes with 4 μM Cal-520 (AAT Bioquest), and transferred 2 x 104 cells per well into flat-bottom 96 well plates coated with a CD3 antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... T-tubules from intact hearts were stained with MM 4-64 (2.5 μM, AAT BioQuest) in Ca2+ free Tyrode solution via Langendorff perfusion at room temperature for 30 min as described previously (53 ...
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bioRxiv - Immunology 2024Quote: ... The standard curve was created with an online 4-PL curve calculator from AAT Bioquest (https://www.aatbio.com/tools/four-parameter-logistic-4pl-curve-regression-online-calculator) ...
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bioRxiv - Cell Biology 2023Quote: ... and fluorescein-PE [N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine] was obtained from AAT Bioquest Inc.
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bioRxiv - Neuroscience 2023Quote: iDANoids were incubated for 30 min at 37 °C with 3 µM Calbryte 520 AM calcium indicator (AAT Bioquest) in maturation medium containing 0.02% Pluronic F-127 (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: Cell samples were incubated with the calcium indicator dye (4 mM of Cal-520 AM, AAT Bioquest) for 1 hr ...
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bioRxiv - Genomics 2024Quote: Fluorescent probes were generated using the Nick translation DNA labeling system 2.0 (Enzo) with XFD 488-dUTP or Cyanine-3-dUTP (AAT Bioquest). Unincorporated nucleotides were removed using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation with the Ca2+ indicator dye Fluo-4 AM (10 μM, AAT Bioquest, Sunnyvale, CA, USA) for another 30-45 min at 37°C ...
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bioRxiv - Biophysics 2022Quote: ... #14175103)] followed by incubation for 1 hour at 37°C and 5% CO2 in 1800 µl of imaging buffer containing 3 mM Cal-520-AM (AAT Bioquest; #21130) Cal-520-AM-loaded cells were removed from the CO2 incubator and equilibrated at room temperature for 5 minutes prior to IP3 stimulation by the addition of 200 µl of 1 mM carbachol (Alfa Aesar ...
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bioRxiv - Physiology 2024Quote: ... and incubated at 37°C under continued static or FSS conditions for 3 hours with 50-1000 nM Texas Red gentamicin conjugate (GTTR; AAT Bioquest, 24300) apically in DF+H medium ...
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bioRxiv - Neuroscience 2022Quote: ... primary microglia of indicated groups were cultured in heat-inactivated DMEM medium containing 5 μM Fluo-4 AM (AAT Bioquest), 0.4 % F-127 (Beyotime ...
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bioRxiv - Biophysics 2020Quote: ... culture medium were discarded and cells were incubated with 50 μl per well of 4 μM Rhod-2/AM (AAT Bioquest) in TARODES’s buffer (10 mM HEPES ...
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bioRxiv - Plant Biology 2023Quote: ... T50 was calculated by fitting a 4-parameter logistic equation to the dataset using the calculator at (AAT BioQuest Inc, 2022), see example in Figure S4 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Attached cells were loaded with the Ca2+ dye Fluo-8 at room temperature (23 – 25 °C) by adding 4 μM Fluo-8 AM (AAT Bioquest) and 0.02% Pluronic F-127 (SiChem ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Neuroscience 2021Quote: ... we loaded the ORNs with a calcium sensitive dextran coupled dye (Cal 520 dextran conjugate, 10 kDa, AAT Bioquest; 3 mM in Frog Ringer) via bulk electroporation (Weiss et al. ...
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bioRxiv - Physiology 2022Quote: To analyze voltage changes in the SAN preparation we loaded it by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator Di-4-ANEPPS (2 μmol/L; AAT Bioquest, Sunnyvale, California). This immersion was performed at room temperature (20-22°C ...
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bioRxiv - Microbiology 2020Quote: ... samples were incubated for 5 min with 1.2 µg ml-1 of the membrane dye MM 4-64 (AAT Bioquest Sunnyvale, CA) and 0.5 µg ml-1 of 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2021Quote: Neurons were incubated in a calcium indicator dye solution consisting of 5 μM Rhod-4 AM (AAT Bioquest #21122; Sunnyvale, CA, USA), 1X PowerLoad (Thermo Fisher Scientific #P10020) ...
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bioRxiv - Immunology 2024Quote: ... then fit to a sigmoidal 4 parameter logistic (4PL) model by utilizing the Four Parameter Logistic (4PL) Curve Calculator (AAT Bioquest Inc.). The sigmoidal 4PL equations were then used to calculate the concentration of individual samples ...
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bioRxiv - Microbiology 2020Quote: The cell length and width were calculated by first staining the cell with the membrane dye MM 4-64 (AAT Bioquest Sunnyvale, CA) to visualize the cell contour ...
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bioRxiv - Biophysics 2019Quote: ... we measured conduction velocity in well-connected cardiac cultures and co-cultures utilizing the dye Rhod-4-AM (10 µM: AAT Bioquest, Sunnyvale, CA). Rhod-4 AM diluted in Tyrode’s solution containing the following in mM ...
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bioRxiv - Neuroscience 2021Quote: ... we placed dried dye crystals of fluorophore-coupled dextrans (Alexa Fluor dextran 488, 594, Cascade Blue dextran, Thermo Fisher or Cal520 dextran conjugate, AAT Bioquest, 10kDa, 3 mM in Frog Ringer) in the nostrils and applied six electric square pulses using two platinum electrodes (15 V ...