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Citations for anatrace :
1 - 50 of 51 citations for Protein Crystallization since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Concentrated protein was screened for crystallization with the MCSG Crystallization Suite (Anatrace). Long ...
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bioRxiv - Microbiology 2021Quote: ... Initial screening for crystallization conditions was performed using MCSG I-IV crystallization screens (Anatrace). The optimized crystals were obtained using the vapor diffusion method in 20% PEG3350 ...
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bioRxiv - Immunology 2024Quote: Initial crystallization screening was performed by sitting-drop vapor-diffusion using the MCSG Crystallization Suite (Anatrace) using an NT8 drop setter (Formulatrix) ...
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bioRxiv - Biochemistry 2023Quote: ... using the MCSG-1 crystallization screen (Anatrace). The crystal was cryoprotected by addition of 10 volumes of 3.4 M sodium malonate ...
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: Native and selenomethionine-labeled LapD2 was concentrated to 10 mg/ml and screened for crystallization conditions using the MCSG1-4 crystallization suites (Anatrace) and the hanging drop vapour diffusion method ...
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bioRxiv - Biochemistry 2023Quote: ... Initial crystals formed in the MCSG-2 crystallization screen (Anatrace) and optimized crystals were grown in 100 mM sodium acetate ...
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bioRxiv - Biochemistry 2024Quote: ... crystal conditions screened using the MCSG2 crystallization kit (Anatrace Products). X-ray quality crystals grew after 24 hours at 4 ℃ using the condition ...
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bioRxiv - Biochemistry 2023Quote: Initial crystallization conditions were established using the MCSG screen from Anatrace (USA using 6 mg/ml of Cagα and 1 mM of 1G2 (1:10 ratio) ...
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bioRxiv - Biochemistry 2020Quote: ... Initial screening was done using the MCSG Crystallization Suite (Anatrace, Maumee, OH). This initial screening produced the P212121 crystals that were grown in 200 mM NH4 tartrate and 20% PEG 3350 ...
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bioRxiv - Biochemistry 2024Quote: ... BtuG1 was screened for crystals using the MCSG1 crystallization kit (Anatrace Products). Several hits were observed after 24 hours at 4℃ ...
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bioRxiv - Immunology 2021Quote: ... Initial crystal screening was performed by sitting-drop vapor-diffusion in the MCSG Crystallization Suite (Anatrace) using a NT8 drop setter (Formulatrix) ...
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bioRxiv - Microbiology 2023Quote: Native TelALXG-LapA3-LapA4 complex at 10 mg/ml was screened for crystallization conditions using the hanging drop vapor diffusion method and the MCSG suite of sparse matrix crystal screens (Anatrace). Crystals formed after one to two weeks in 0.02M MgCl2 ...
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bioRxiv - Biochemistry 2024Quote: ... BtuG2 was concentrated to 22 mg/mL prior to substrate addition and screened for crystals using the MCSG3 crystallization kit (Anatrace Products). Several hits were observed after 48 hours at 4 ℃ using the following condition ...
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bioRxiv - Biochemistry 2022Quote: ... To reconstitute MBOAT7 proteins in PMAL-C8 (Anatrace), MBOAT7 in detergent was mixed with PMAL-C8 at a 1:3 (w:w ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was extracted by incubating with 1% GDN (Anatrace) on a rotator for 2 hours at 4C ...
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bioRxiv - Biophysics 2022Quote: SEC-purified protein [in SEC buffer containing 3 mM DM (Anatrace)] was reconstituted into liposomes ...
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bioRxiv - Biophysics 2022Quote: ... Protein was solubilized with the addition of 0.1% (v/v) DDM/CHS (Anatrace) for 2 hours at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... The protein was extracted by adding n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) to a final concentration of 2% ...
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bioRxiv - Cell Biology 2023Quote: ... Expressed protein was solubilized in 2 % lauryl maltose neopentyl glycol (LMNG) (Anatrace, NG310), 0.2 % Cholesteryl Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Neuroscience 2024Quote: ... protein was extracted from the pellet with 0.5% Fos-choline 14 (#F312, Anatrace) at 4°C for 40 minutes and centrifuged at 21 000 g for 1 hour at 4°C ...
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bioRxiv - Biophysics 2020Quote: 3.5 μL of protein-embedded nanodiscs supplemented with 1.5 mM Fos-Choline-8 (Anatrace) was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3 ...
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bioRxiv - Plant Biology 2021Quote: Thylakoids (100 µg of proteins) were solubilized with 0.75% n-Dodecyl-α-D-maltoside (Anatrace) at RT for 20 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The protein was extracted by 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... Protein extraction was carried out with 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace #D322S25GM) for 2 hr at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... protein was extracted with 2% (w/v) decyl-ý-D-maltopyranoside (DM; Anatrace, Maumee, OH) for 2 h at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Outer-membrane proteins were solubilized using 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in a buffer containing 20 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Neuroscience 2021Quote: ... Protein was extracted with a final concentration of 1% n-dodecyl-β-D-maltopyranoside (DDM; Anatrace), 0.2% cholesteryl hemisuccinate (CHS ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... Protein was extracted by addition of 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.1% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was solubilized by addition of 1% w/v n-decyl-β-D-maltopyranoside (DM) (Anatrace) and 0.1% w/v cholesteryl hemisuccinate (CHS ...
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bioRxiv - Cell Biology 2021Quote: His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
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bioRxiv - Biochemistry 2019Quote: ... and membrane proteins were extracted by stirring with 30 mM n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was concentrated to 10 mg/ml using a Proteus X-spinner with 10 kDa filter (Anatrace) and snap frozen in liquid nitrogen ...
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bioRxiv - Biophysics 2021Quote: ... membrane proteins were extracted from the cell with 1% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2019Quote: ... Membrane proteins were solubilized and PEGylated by adding 7 μL of 10% DDM (β-D-dodecyl maltoside, Anatrace) and 13 μL of 27mM mal-PEG (Methoxypolyethylene glycol maleimide ...
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bioRxiv - Biophysics 2020Quote: ... The mutant CLC-ec1 proteins were extracted with n-decyl-β-D-maltopyranoside (DM, Anatrace; 2% (w/v)) and allowed to bind to cobalt resin (Talon ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane proteins were extracted by supplementing the lysis buffer with 1% n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), and incubated for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Membrane proteins were solubilized and PEGylated by adding 7 µl of 10% β-d-dodecyl maltoside (Anatrace 329370010) and 13 µl of freshly dissolved 27 mM 5 kDa mal-PEG ...
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bioRxiv - Biophysics 2022Quote: ... the membrane fraction was prepared by ultracentrifugation at 100,000 x g for one hour and protein was solubilized by addition of lysis buffer containing 0.5 % LMNG (Anatrace) for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the GLUT3 protein was purified in 0.06% (w/v) 6-cyclohexyl-1-hexyl-ß-D-maltoside (Cymal-6, Anatrace) and concentrated to ~ 40 mg/ml ...
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bioRxiv - Biophysics 2020Quote: ... Concentrated PilQ (150 µL of ∼1 mg/mL protein) was exchanged into Amphipol A8-35 (0.585 mg for a 3:1 ratio, Anatrace, A835) and allowed to incubate at 4 C for 1 hr ...
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bioRxiv - Biophysics 2021Quote: A total of 4.0 μl of 20 μM TpCorC protein in 20 mM HEPES (pH 7.5) containing 0.03% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, USA) and either 150 mM NaCl or 150 mM KCl was mixed with 0.5 μl of 2 mM EDTA ...
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bioRxiv - Biophysics 2022Quote: ... The protein was extracted by adding 50 mM n-Dodecyl-β-D-maltopyranoside with 0.25% cholesteryl hemisuccinate (Anatrace, D310-CH210) and nutating for 1 hr at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Protein was extracted via the addition of n-Dodecyl-β-D-maltopyranoside (DDM) with cholesteryl hemisuccinate (CHS) (Anatrace, D310-CH210) to a final concentration of 50 mM DDM / 4 mM CHS and rotated for 1 hour at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... membranes at a protein concentration of ∼4 mg/ml were solubilized with 1% (w:v) n-dodecyl-β-d-maltopyranoside (DDM) (Anatrace) and 0.1% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Giβ1ψ2 G-protein were lysed in hypotonic buffer and G-protein was extracted in a buffer containing 1% sodium cholate and 0.05% n-dodecyl-β-D-maltoside (DDM, Anatrace). Detergent was exchanged from cholate/DDM to DDM on Ni Sepharose column ...
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bioRxiv - Biophysics 2022Quote: ... and the membrane pellet was resuspended in Lysis buffer and the protein was solubilized with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.04% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 200 μg/ml purified proteins were added into liposomes after incubation with 1% n-octyl-ß-D-glucopyranoside (ß-OG, Anatrace) at 4 °C for 30 min ...
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bioRxiv - Biophysics 2020Quote: ... The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace, Maumee, OH) and incubating for 1 h at 4° C ...
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bioRxiv - Biophysics 2021Quote: ... proteins were overexpressed in BL21-AI E.coli competent cells and extracted into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris(2-carboxyethyl)phosphine ...
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bioRxiv - Biophysics 2023Quote: ... coli cells transformed with the expression plasmid were lysed by sonication and the protein extracted from membrane fragments into 2% n-Decyl-β-D-Maltopyranoside (DM; Anatrace, Maumee OH) containing 5 mM TCEP (Tris (2-carboxyethyl ...