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Citations for anatrace :
1 - 50 of 55 citations for 8 4 chlorophenyl 8 oxooctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: Fluorinated fos-choline 8 (Anatrace) was added into 4 mg/ml α2β heteromer saposin nanodisc sample at a final concentration of 1 cmc just before freezing grids ...
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bioRxiv - Biophysics 2020Quote: ... Fluorinated Fos-choline-8 (Anatrace) was added to the protein sample to a final concentration of 3 mM ...
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bioRxiv - Biochemistry 2023Quote: ... fluorinated Fos-Choline-8 (Anatrace) dissolved in water was added to the sample to a final concentration of 1.5 mM ...
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bioRxiv - Biochemistry 2022Quote: ... 3 μM Fos-Choline-8 (Anatrace) was added to the protein sample ...
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bioRxiv - Microbiology 2021Quote: ... and 1mg/ml Amphipol 8-35 (Anatrace). Buffer exchange into TBS pH7.4 and 1 mg/ml Amphipol 8-35 using Amicon Ultra-4 centrifugation filters with a 100kDa cutoff (Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorinated fos-choline 8 (Anatrace, cat# F300F) was added to T ...
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bioRxiv - Biophysics 2020Quote: ... 3 mM fluorinated Fos-choline 8 (Anatrace, USA) was added to improve particle distribution in vitreous ice ...
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bioRxiv - Biochemistry 2021Quote: ... and fluorinated fos-choline 8 (FC8) were from Anatrace. Cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biophysics 2022Quote: ... 8% (wt/vol) n-octyl-b-D-maltopyranoside (OM, Anatrace) was added to solubilize the lipids ...
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bioRxiv - Biophysics 2022Quote: ... and 2.5 mM fluorinated fos-choline-8 (Anatrace Cat# F300F), a detergent that does not interact with hydrocarbons ...
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bioRxiv - Biophysics 2023Quote: ... The addition of 0.75 mM fluorinated Fos-choline-8 (Anatrace) resulted in even ice distribution.
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bioRxiv - Biochemistry 2020Quote: Purified samples were supplemented with 3 mM fluorinated Fos-Choline-8 (FFC8; Anatrace) before plunge freezing ...
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bioRxiv - Biophysics 2020Quote: 3.5 μL of protein-embedded nanodiscs supplemented with 1.5 mM Fos-Choline-8 (Anatrace) was applied to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3 ...
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bioRxiv - Cell Biology 2022Quote: ... at ~5 mg/mL in SEC Buffer containing 2 mM fluorinated fos-choline-8 (Anatrace, cat# F300F), was applied to glow discharged Quantifoil R1.2/1.3 grids ...
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bioRxiv - Biophysics 2020Quote: ... 3.5 μL of GltPh-containing nanodiscs (7 mg/mL) supplemented with 1.5 mM fluorinated Fos-Choline-8 (Anatrace) were applied to a glow-discharged UltrAuFoil R1.2/1.3 300-mesh gold grid (Quantifoil ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µL of 8 mg/ml complex with 3 mM CHAPSO or 0.01% fluorinated octyl-maltoside (FOM) (Anatrace) were applied onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysate was dounce homogenized (8 strokes with the A pestle) and a 1% Dodecyl maltoside (DDM)/0.2% Cholesteryl hemisuccinate (CHS) (Anatrace) w:w mixture was added for protein solubilization ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 8 column buffers of GTBS (50 mM Tris-HCl, 150 mM NaCl, 0.006 % (w/v) glyco-diosgenin (GDN, Anatrace), pH 8.0) ...
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bioRxiv - Biophysics 2021Quote: ... purified protein at a concentration of 6–7 mg/mL was mixed with 2.9 mM Fluorinated Fos-Choline-8 (FFC8) (Anatrace) immediately prior to grid preparation ...
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bioRxiv - Biophysics 2022Quote: ... Cryo-EM grids of anaerobically-purified FHL were prepared similarly within the anaerobic chamber except that 0.5x CMC fluorinated fos-choline-8 (Anatrace) was added immediately before the sample was applied to the grid ...
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bioRxiv - Biochemistry 2024Quote: 3.5 uL of nhTMEM16-containing nanodiscs (3-7mg ml-1) supplemented with 1.5 or 3 mM Fos-Choline-8-Fluorinated (Anatrace) was applied to a glow-discharged UltrAuFoil R1.2/1.3 300-mesh gold grid (Quantifoil ...
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bioRxiv - Biochemistry 2024Quote: ... Nanodiscs were formed with a mixture of phospholipids (48% POPC, 32% POPE, 12% DOPS, 8% cardiolipid (w/w)) (Anatrace). The cell-free synthesis protocol was detailed previously [32,36,70] ...
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bioRxiv - Biochemistry 2020Quote: ... with or without 1.5 mg/ml cardiolipin was prepared in water and solubilized using 8% (w/vol) n-octyl-β-D-maltopyranoside (Anatrace). Protein that had been purified by SEC in 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2024Quote: ... 2.7 µL of sample was mixed with 0.3 µL of fluorinated fos-choline-8 solution (Anatrace; final concentration 1.5 mM) and applied to UltrAuFoil 0.6/1 300 mesh gold grids (Quantifoil) ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 8 column buffers of GTBS (50 mM Tris HCl, 150 mM NaCl, 0.006% [w/v] glyco-diosgenin [GDN; Anatrace], pH 8.0). Ycf1p was eluted with 6 column volumes of GTBS containing 150 μg/mL 3×FLAG peptide ...
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bioRxiv - Immunology 2023Quote: ... the complex was extracted from the membrane with 0.8% (w/v) lauryl maltose neopentylglycol (LMNG; Anatrace, CAS. No. 1423315-02-8) and 0.1% (w/v ...
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bioRxiv - Biophysics 2019Quote: ... 4 mM Cholesteryl Hemisuccinate (CHS, Anatrace) and 2 μM RTx and incubated at 4° C for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... The resuspension was solubilized at 4 °C for 4 h with 2% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... 6 mM amino acid mixture (average concentration of each amino acid 0.3 mM) and 0.1% (w/v) MNG-3 (Maltose Neopentyl Glycol-3, Anatrace). The feeding buffer contained 1x SUB-AMIX buffer (CellFree Sciences Japan) ...
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bioRxiv - Biophysics 2023Quote: ... and then solubilized with 4% n-octyl-β-D-glucopyranoside (OG; Anatrace). Solubilized AQP0 was purified using anion-exchange (MonoQ ...
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bioRxiv - Biochemistry 2020Quote: ... was added before cruel-extraction at 4°C in 1.5% DDM and 0.3% CHS (Anatrace) for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... The plasma membranes were solubilized at 4 °C with 10x critical micelle concentration (CMC) DDM (Anatrace) in a solution of 20mM HEPES (pH8.0) ...
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bioRxiv - Biophysics 2022Quote: DDM-solubilized claudin-4 was exchanged into 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside (LMNG, Anatrace) or amphipol A8-35 (Anatrace) ...
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bioRxiv - Biophysics 2023Quote: Cells were solubilized for 1 hour at 4 °C in 1% (w/v) Glyco-diosgenin (GDN) (Anatrace). Insoluble material was removed by centrifugation at 16,000 rpm for 30 minutes at 4 °C ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... 4 mL (1/10 volume) of 1 M n-octyl-β-D-glucopyranoside in H2O (β-OG, Anatrace) was added to 100 mM final concentration ...
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bioRxiv - Biochemistry 2021Quote: ... Nanodiscs were assembled on the resin by adding 1.25mM lipids (POPC/DOPE (Avanti) at a 4:1 ratio in DDM (Anatrace)) and 25uM nanodisc-scaffolding protein MSP1D1 in 700ul of washing buffer ...
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bioRxiv - Biophysics 2019Quote: ... at 4 °C for 2 h and then washed with 30 bed volumes of lysis buffer added 0.05% GDN (Anatrace). The protein was eluted with lysis buffer added 0.05% GDN and 300 μg ml-1 Flag peptide (Genscript) ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... or concentrated to 4 mg/mL and vitrified in the presence of 1.5 mM fluorinated fos-choline (FFosC, Anatrace). 3 µL samples were applied to freshly glow-discharged Cu 200 Holey Carbon R1.2/1.3 grids (Quantifoil ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT and incubated 1h at 4 °C with PMAL-C8 (Anatrace, mass ratio protein:PMAL-C8 1:2.25). 30 mg BioBeads (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was solubilized for 3 hours at 4 °C with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... Then the membrane was solubilized for 3 h at 4 °C by adding 0.5% (w/v) lauryl maltoseneopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris were removed by low-speed centrifugation and membranes were isolated through ultracentrifugation (100,000 × g, 1 h, 4°C) and solubilised with 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in buffer I by gentle shaking (4°C ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Solubilization of 8S complexes was performed by mixing the homogenized sample at 4°C for 2 hours after addition of 2% C12M (Anatrace). After removing the insoluble material by centrifugation at 42,000 rpm (Ti-45 rotor ...
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bioRxiv - Biochemistry 2022Quote: ... UBR5 was frozen in GF Buffer with the addition of 4 mM CHAPSO or 0.005 % fluorinated octyl β-maltoside (Anatrace) prior to freezing.
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: Native and selenomethionine-labeled LapD2 was concentrated to 10 mg/ml and screened for crystallization conditions using the MCSG1-4 crystallization suites (Anatrace) and the hanging drop vapour diffusion method ...
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bioRxiv - Biophysics 2023Quote: ... membranes at a protein concentration of ∼4 mg/ml were solubilized with 1% (w:v) n-dodecyl-β-d-maltopyranoside (DDM) (Anatrace) and 0.1% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...