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Citations for anatrace :
1 - 50 of 355 citations for 7 PHENYL 1 2 4 TRIAZOLO 1 5 A PYRIMIDINE 6 CARBONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and 6-cyclohexyl-1-hexyl-β-D-maltoside (Cymal-6) (Anatrace). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: ... 5-6 mM LMNG (Anatrace) and 1-1.2 mM Cholesteryl Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL of solubilization solution supplemented with the 2 % 6-Cyclohexyl-1-Hexyl-β-D-Maltoside (Cymal6, Anatrace) was added gently to the membrane solution and the sample incubated at RT for 30 min under moderate shaking ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% (wt/vol) Cymal-5 (Anatrace), and a cocktail of protease inhibitors (Roche Complete EDTA-free tablets) ...
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bioRxiv - Biophysics 2022Quote: ... 1 mg mL−1 KcsA in 5 mM decyl-β-maltopyranoside (Anatrace) detergent (DM ...
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bioRxiv - Microbiology 2023Quote: ... Cymal-5 and Cymal-6 were purchased from Anatrace, RIPA buffer from ThermoFisher and Superflow Ni-NTA from Bio-Rad.
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the GLUT3 protein was purified in 0.06% (w/v) 6-cyclohexyl-1-hexyl-ß-D-maltoside (Cymal-6, Anatrace) and concentrated to ~ 40 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: A mixture of 2.75:1.25:1 mg of chloroform-dissolved 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphoethanolamine (POPE): 1-Palmitoyl-2-Oleoyl-sn-Glycero-3-Phosphoglycerol (POPG):cholesterol (Anatrace) were dissolved and mixed in chloroform ...
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bioRxiv - Biophysics 2020Quote: ... 5 mM Na-Asp and 7 mM n-decyl-β-D-maltopyranoside (C10M; Anatrace). For GltPh-XL2 and GltPh-XL3 ...
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bioRxiv - Biophysics 2023Quote: Cells were solubilized for 1 hour at 4 °C in 1% (w/v) Glyco-diosgenin (GDN) (Anatrace). Insoluble material was removed by centrifugation at 16,000 rpm for 30 minutes at 4 °C ...
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bioRxiv - Biophysics 2021Quote: Purified megabody was added at 1:1.5 molar ratio and 0.05 % (w/v) CYMAL-7-NG (Anatrace) and 0.01 mg/mL 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC ...
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bioRxiv - Biophysics 2021Quote: ... purified protein at a concentration of 6–7 mg/mL was mixed with 2.9 mM Fluorinated Fos-Choline-8 (FFC8) (Anatrace) immediately prior to grid preparation ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... 4 mL (1/10 volume) of 1 M n-octyl-β-D-glucopyranoside in H2O (β-OG, Anatrace) was added to 100 mM final concentration ...
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bioRxiv - Biophysics 2021Quote: ... 3-([3-cholamidopropyl]dimethylammonio)-2-hydroxy-1-propanesulfonate (CHAPSO, Anatrace) was added to the sample (8 mM final) ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM EDTA) containing 1% Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and supplemented with cOmplete™ protease inhibitor cocktail (Roche) ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT and incubated 1h at 4 °C with PMAL-C8 (Anatrace, mass ratio protein:PMAL-C8 1:2.25). 30 mg BioBeads (BioRad ...
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bioRxiv - Biochemistry 2022Quote: ... Drops of 450 nL of 10 mg/mL protein in SEC buffer and 150 nL of MCSG-1 H10 (Anatrace; 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris were removed by low-speed centrifugation and membranes were isolated through ultracentrifugation (100,000 × g, 1 h, 4°C) and solubilised with 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in buffer I by gentle shaking (4°C ...
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bioRxiv - Biophysics 2019Quote: CHAPSO {3-([3-cholamidopropyl]dimethylammonio)-2-hydroxy-1-propanesulfonate} (Anatrace, Maumee, OH) was added to the samples to a final concentration of 8 mM (Chen et al. ...
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bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM cholesteryl hydrogen succinate (MP Biomedicals ...
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bioRxiv - Biochemistry 2022Quote: 2.5mg of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) in chloroform (Anatrace) were dried for 3h under argon flow ...
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bioRxiv - Biophysics 2021Quote: ... 2 mM pepstatin A (which are all protease inhibitors) plus 1% GDN detergent (Anatrace). Protein was extracted from the membrane by whole-cell solubilization for 1 h at 4 °C with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... 200 mM NaCl, 2 mM MgAc2, 1x cOmpleteTM EDTA-free Protease Inhibitor Cocktail [Roche], 1% [w/v] glyco-diosgenin [GDN; Anatrace], 1 mM DTT) at a ratio 6.8 ml solubilization buffer per 1 g cell pellet ...
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bioRxiv - Biochemistry 2024Quote: ... The resuspension was solubilized at 4 °C for 4 h with 2% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% (w/v ...
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bioRxiv - Genetics 2023Quote: ... The pellet was resuspended in 5 ml lysis buffer containing 1% DDM (Anatrace, Maumee, OH, USA) and rotated for 2 h at 4°C to extract the membrane proteins ...
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bioRxiv - Biochemistry 2023Quote: ... 5 % (v/v) glycerol) and solubilized with 1 % (w/v) lauryl maltose neopentyl glycol (LMNG) (Anatrace) and 0.1 % (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Solubilization of 8S complexes was performed by mixing the homogenized sample at 4°C for 2 hours after addition of 2% C12M (Anatrace). After removing the insoluble material by centrifugation at 42,000 rpm (Ti-45 rotor ...
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bioRxiv - Biophysics 2020Quote: ... 5 μg/μL leupeptin and 2% n-dodecyl-β-D-maltopyranoside (DDM, Anatrace), mixed using a Dounce homogenizer and incubated for ~16 hr at 4°C with agitation ...
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bioRxiv - Molecular Biology 2019Quote: TcA (1 mg/ml) was mixed with different lipid mixes dissolved in 5% β-D-octylglucoside (Anatrace) with a total lipid concentration of 5 mg/ml ...
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bioRxiv - Plant Biology 2024Quote: ... 10 μM E-64 and 5 mM Benzamidine HCl) containing 1% Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% cholesteryl hemisuccinate (CHS ...
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bioRxiv - Biochemistry 2020Quote: TRPC4 membranes were solubilized for 2 hr in buffer B supplemented with 1% LMNG (Anatrace #NG310). Then a protocol similar to that used for DDM purification was followed ...
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bioRxiv - Biochemistry 2020Quote: Membranes were solubilized for 2 hr in buffer B supplemented with 1% dodecyl maltoside (Anatrace #D310). Insoluble material was removed by ultracentrifugation for 1 hr in a Beckman Coulter Type 70 Ti rotor at 40,000 rpm ...
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bioRxiv - Biochemistry 2019Quote: ... then treated with 1 uL of sodium 2-sulfonatoethyl methanethiosulfonate (MTSES, Anatrace, 10x stock in water) to a working concentration of 100 uM ...
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bioRxiv - Molecular Biology 2021Quote: ... Cymal-6 (Anatrace) was added to the Env(- ...
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bioRxiv - Biophysics 2021Quote: ... then incubated for 1 hour in the same resuspension buffer supplemented with 2 % DDM (Anatrace, OH, USA) and 0.1 mg/ml cardiolipin ...
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bioRxiv - Biochemistry 2021Quote: ... Nanodiscs were assembled on the resin by adding 1.25mM lipids (POPC/DOPE (Avanti) at a 4:1 ratio in DDM (Anatrace)) and 25uM nanodisc-scaffolding protein MSP1D1 in 700ul of washing buffer ...
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bioRxiv - Biochemistry 2023Quote: ... alcyone ACE2 dimer with 7 mM CHAPSO (Anatrace) were applied and blotted twice as previously described88 ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Biophysics 2023Quote: ... membranes at a protein concentration of ∼4 mg/ml were solubilized with 1% (w:v) n-dodecyl-β-d-maltopyranoside (DDM) (Anatrace) and 0.1% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed in ATF6 lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 5 mM EDTA, 1x protease inhibitor, and 1% LMNG (Anatrace NG322) for 15 min at 4°C as previously described68 ...
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bioRxiv - Biochemistry 2023Quote: ... for rounds 2-5 phage particles were eluted from magnetic beads by a 15-min incubation step with 1% Fos-choline-12 (Anatrace) prepared in buffered saline.
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
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bioRxiv - Cell Biology 2021Quote: ROS of 50 bovine retinas were isolated as described previously and suspended in Extraction Buffer (20 mM BTP, pH 7.9, 10% glycerol, 300 mM NaCl, 1 mM TCEP) containing 2% LMNG (Anatrace) (Papermaster ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) using a Dounce homogenizer and this membrane suspension was solubilized with 1% dodecyl-maltoside (DDM, Anatrace) and 0.1% cholesteryl hemi-succinate (CHS ...
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bioRxiv - Biochemistry 2023Quote: ... 1,2-Dimyristoyl-sn-glycerol-3-phosphocholine (DMPC) and 3((3-cholamidopropyl)- dimethylammonio)-2-hydroxy-1-propanesulphonate (CHAPSO) were purchased from Anatrace. Sodium dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2022Quote: ... at ~5 mg/mL in SEC Buffer containing 2 mM fluorinated fos-choline-8 (Anatrace, cat# F300F), was applied to glow discharged Quantifoil R1.2/1.3 grids ...
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bioRxiv - Biochemistry 2021Quote: ... 1% DMNG (Anatrace) for 1hr ...
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bioRxiv - Biochemistry 2021Quote: ... and 5 mM BME and further solubilized by stirring with the addition of 1% n-dodecyl-β-D-maltopyranoside (DDM) (Anatrace, OH) for an hour at 4 °C ...