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Citations for anatrace :
1 - 50 of 287 citations for 6 1 FLUOROETHYL 4 1H PYRIMIDINONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 1 mM DTT and incubated 1h at 4 °C with PMAL-C8 (Anatrace, mass ratio protein:PMAL-C8 1:2.25). 30 mg BioBeads (BioRad ...
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bioRxiv - Biophysics 2023Quote: ... For nanodisc samples, 0.5 mM (1H, 1H, 2H, 2H-Perfluorooctyl)-β-D- Maltopyranoside (FOM, Anatrace) was added for improving particles distribution and contrast ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.0, 150 mM NaCl, 10% glycerol), with or without 0.1% (1H, 1H, 2H, 2H-perfluorooctyl)phosphocholine (O310F, Anatrace, USA). HS-AFM observations of GluA2-γ2 in the closed state were performed on mica in buffer A supplemented with 0.1% O310F and 0.3 mM ZK200775 (#2345 ...
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bioRxiv - Biochemistry 2022Quote: ... and 6-cyclohexyl-1-hexyl-β-D-maltoside (Cymal-6) (Anatrace). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the GLUT3 protein was purified in 0.06% (w/v) 6-cyclohexyl-1-hexyl-ß-D-maltoside (Cymal-6, Anatrace) and concentrated to ~ 40 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Cymal-6 (Anatrace) was added to the Env(- ...
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bioRxiv - Molecular Biology 2023Quote: ... The suspension was incubated at RT for 1h and then solubilized by 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace) and 0.1% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... 5-6 mM LMNG (Anatrace) and 1-1.2 mM Cholesteryl Hemisuccinate Tris Salt (CHS ...
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bioRxiv - Biophysics 2021Quote: ... The membrane pellet was solubilized at 4 °C for 4 h with 1% (w/v) n-dodecyl-β-D-maltopyranoside (DDM, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biophysics 2023Quote: Cells were solubilized for 1 hour at 4 °C in 1% (w/v) Glyco-diosgenin (GDN) (Anatrace). Insoluble material was removed by centrifugation at 16,000 rpm for 30 minutes at 4 °C ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... 4 mL (1/10 volume) of 1 M n-octyl-β-D-glucopyranoside in H2O (β-OG, Anatrace) was added to 100 mM final concentration ...
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bioRxiv - Microbiology 2020Quote: ... 20 mL of solubilization solution supplemented with the 2 % 6-Cyclohexyl-1-Hexyl-β-D-Maltoside (Cymal6, Anatrace) was added gently to the membrane solution and the sample incubated at RT for 30 min under moderate shaking ...
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bioRxiv - Microbiology 2019Quote: ... Cell debris were removed by low-speed centrifugation and membranes were isolated through ultracentrifugation (100,000 × g, 1 h, 4°C) and solubilised with 1% (w/v) n-dodecyl-β-d-maltopyranoside (DDM; Anatrace) in buffer I by gentle shaking (4°C ...
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bioRxiv - Biophysics 2020Quote: ... 6% deoxy Big-CHAP (Affymetrix Anatrace, Maumee, OH, USA) and SecYEG in detergent (Protein to Lipid ratio of 1:50 ...
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bioRxiv - Microbiology 2023Quote: ... Cymal-5 and Cymal-6 were purchased from Anatrace, RIPA buffer from ThermoFisher and Superflow Ni-NTA from Bio-Rad.
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bioRxiv - Biophysics 2023Quote: ... the cells were washed and solubilized for 1 h at 4 °C with buffer containing 1% lauryl maltose neopentyl glycol (Anatrace, Maumee, OH, USA), 2 mM cholesteryl hydrogen succinate (MP Biomedicals ...
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bioRxiv - Biochemistry 2021Quote: ... Nanodiscs were assembled on the resin by adding 1.25mM lipids (POPC/DOPE (Avanti) at a 4:1 ratio in DDM (Anatrace)) and 25uM nanodisc-scaffolding protein MSP1D1 in 700ul of washing buffer ...
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bioRxiv - Biophysics 2019Quote: ... 4 mM Cholesteryl Hemisuccinate (CHS, Anatrace) and 2 μM RTx and incubated at 4° C for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... and protease inhibitors) and solubilized at 4 °C for 2 hours in a buffer containing 1% lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% cholesteryl hemisuccinate tris salt (CHS ...
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bioRxiv - Biochemistry 2020Quote: ... then gently rotated for 2 hours at 4°C (lysis buffer: 50 mM HEPES pH 7.5, 150 mM NaCl, 1% w/v DDM (Anatrace, solgrade), 1 mM DTT ...
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bioRxiv - Biophysics 2023Quote: ... membranes at a protein concentration of ∼4 mg/ml were solubilized with 1% (w:v) n-dodecyl-β-d-maltopyranoside (DDM) (Anatrace) and 0.1% cholesteryl hemisuccinate Tris salt (CHS ...
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bioRxiv - Biophysics 2020Quote: ... After lysis with 2 cycles of 20,000 psi using an Avestin Emulsiflex C5 membrane fractions were separated by centrifugation at 100,000xg for 1 hour at 4° C and membrane pellets were solubilized in Lysis buffer containing 1% DDM (n-Dodecyl-β-D-maltopyranoside, Anatrace). Following incubation for 1 hour at 4° C solutions were centrifuged at 100,000xg for 30 minutes at 4° C ...
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bioRxiv - Biochemistry 2024Quote: ... The resuspension was solubilized at 4 °C for 4 h with 2% (w/v) Lauryl Maltose Neopentyl Glycol (LMNG, Anatrace) and 0.2% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... n-dodecyl-b-D-maltoside (DM, Cat. D310, CAS 69227-93-6) was from Anatrace. Purified rabbit IgG was from Bethyl (P120-101) ...
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bioRxiv - Biochemistry 2022Quote: ... Drops of 450 nL of 10 mg/mL protein in SEC buffer and 150 nL of MCSG-1 H10 (Anatrace; 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES ...
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bioRxiv - Biophysics 2021Quote: ... purified protein at a concentration of 6–7 mg/mL was mixed with 2.9 mM Fluorinated Fos-Choline-8 (FFC8) (Anatrace) immediately prior to grid preparation ...
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bioRxiv - Neuroscience 2023Quote: ... on a Superose 6 column in SEC buffer (20 mM HEPES pH 8.0, 150 mM NaCl, 0.05 % DDM detergent (Anatrace D310), and 50 mM glutamate).
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bioRxiv - Biophysics 2023Quote: ... and then solubilized with 4% n-octyl-β-D-glucopyranoside (OG; Anatrace). Solubilized AQP0 was purified using anion-exchange (MonoQ ...
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bioRxiv - Biochemistry 2020Quote: ... was added before cruel-extraction at 4°C in 1.5% DDM and 0.3% CHS (Anatrace) for 2 hours ...
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bioRxiv - Neuroscience 2020Quote: ... The plasma membranes were solubilized at 4 °C with 10x critical micelle concentration (CMC) DDM (Anatrace) in a solution of 20mM HEPES (pH8.0) ...
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bioRxiv - Biophysics 2022Quote: DDM-solubilized claudin-4 was exchanged into 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside (LMNG, Anatrace) or amphipol A8-35 (Anatrace) ...
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bioRxiv - Biophysics 2019Quote: ... at 4 °C for 2 h and then washed with 30 bed volumes of lysis buffer added 0.05% GDN (Anatrace). The protein was eluted with lysis buffer added 0.05% GDN and 300 μg ml-1 Flag peptide (Genscript) ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... or concentrated to 4 mg/mL and vitrified in the presence of 1.5 mM fluorinated fos-choline (FFosC, Anatrace). 3 µL samples were applied to freshly glow-discharged Cu 200 Holey Carbon R1.2/1.3 grids (Quantifoil ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was solubilized for 3 hours at 4 °C with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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Molecular insights into the atypical activation mechanism of GPR156 in maintaining auditory functionbioRxiv - Molecular Biology 2024Quote: ... Then the membrane was solubilized for 3 h at 4 °C by adding 0.5% (w/v) lauryl maltoseneopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Solubilization of 8S complexes was performed by mixing the homogenized sample at 4°C for 2 hours after addition of 2% C12M (Anatrace). After removing the insoluble material by centrifugation at 42,000 rpm (Ti-45 rotor ...
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bioRxiv - Biochemistry 2022Quote: ... UBR5 was frozen in GF Buffer with the addition of 4 mM CHAPSO or 0.005 % fluorinated octyl β-maltoside (Anatrace) prior to freezing.
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Dual targeting factors are required for LXG toxin export by the bacterial type VIIb secretion systembioRxiv - Microbiology 2022Quote: Native and selenomethionine-labeled LapD2 was concentrated to 10 mg/ml and screened for crystallization conditions using the MCSG1-4 crystallization suites (Anatrace) and the hanging drop vapour diffusion method ...
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bioRxiv - Biochemistry 2021Quote: ... 1% DMNG (Anatrace) for 1hr ...
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bioRxiv - Biochemistry 2022Quote: ... diluted with 4 mL GTBS (50 mM Tris-HCl, 150 mM NaCl, 0.004% [w/v] glyco-diosgenin [GDN; Anatrace], pH 7.4) and concentrated to ~200 μL in the same concentrator ...
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bioRxiv - Biophysics 2022Quote: ... 1 mg mL−1 KcsA in 5 mM decyl-β-maltopyranoside (Anatrace) detergent (DM ...
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bioRxiv - Biophysics 2020Quote: ... 1% (w/v) 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS; Anatrace; #C216), and 0.2% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... 1% (w/v) DDM and 1% (w/v) β-D-Ocytlglucoside (Anatrace). Sample aliquots of 100 μl were heated for 10 min over a temperature range of 20–80°C in a PCR thermocycler (Veriti ...
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bioRxiv - Biophysics 2019Quote: ... and 1% C12E8 (Anatrace) for 2 hours at 4°C and ultracentrifuged at 186,000g for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... the receptor was solubilized for 2 hrs at 4°C with continual stirring in a solution of 0.5% L-MNG (Anatrace, Cat. no: NG310) and 0.1% cholesteryl hemisuccinate (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM phenylmethanesulfonyl fluoride (PMSF) and 1% (w/v) glyco-diosgenin (GDN, Anatrace) at 4 °C for 80 min ...
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bioRxiv - Plant Biology 2020Quote: ... at 2.0 mg Chl/mL and solubilized with 4% (w/v) n-dodecyl-α-D-maltoside (α-DDM as previously abbreviated as α-DM; Anatrace) for 30 min with gentle agitation on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% (w/v) GDN (Anatrace), 2 mM PMSF ...
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bioRxiv - Biochemistry 2021Quote: ... 1% (w/v) (DDM (Anatrace) and 0.2% (w/v ...
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Structural insight into guanylyl cyclase receptor hijacking of the kinase–Hsp90 regulatory mechanismbioRxiv - Biochemistry 2023Quote: ... 0.0005% CHS (10:1) (Anatrace), and 5 mM ATP ...