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Citations for Qiagen :
1 - 50 of 5853 citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... miR-329 pLNA (miRCURY LNA miRNA Power Inhibitor RNO-MIR-329-3P, Qiagen # 339130 YI04101481-DCA), miR-495 pLNA (miRCURY LNA miRNA Power Inhibitor HSA-MIR-495-3P ...
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bioRxiv - Neuroscience 2023Quote: ... LNA™ PCR primer set (Qiagen). PCR amplification and detection were performed with the Applied Biosystems StepOnePlus Real-Time detector ...
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bioRxiv - Neuroscience 2022Quote: ... Specimens were incubated for 24 hours at room temperature in the presence of either an ASO antimiR targeting hsa-miR-134-5p (‘Ant-134’; Qiagen, Manchester ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were normalized for the relative expression of the housekeeping miR103a-3p (hsa-miR-103a-3p, LNA™ PCR primer set, Qiagen), which showed no variability between analyzed groups.
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bioRxiv - Immunology 2022Quote: ... SARS-CoV2 subgenomic viral RNA was quantified using primer probe sets as previously described (Wölfel et al., 2020) and Quantifast One-Step RT-PCR master mix (Qiagen) on a QuantStudio 3 or 5 instrument (ThermoFisher) ...
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bioRxiv - Neuroscience 2021Quote: ... double-digoxigenin locked nucleic acid probe (RNO-MIR-124-3P: CATTCACCGCGTGCCTTA, Tm: 84°C, 339111 YD00614870-BGC, miRCURY LNA™ miRNA Detection Probe, Qiagen) was used at a final concentration of 30 nM ...
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bioRxiv - Microbiology 2021Quote: ... One step RT-qPCR for genomic viral RNA was performed using specific primer-probe sets and the QuantiFast Probe RT-PCR +ROX Vial Kit (Qiagen), in the Rotor-Gene Q (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... One step RT-qPCR for both genomic and subgenomic viral RNA was performed using specific primer-probe sets and the QuantiFast Probe RT-PCR +ROX Vial Kit (QIAGEN), in the Rotor-Gene Q (QIAGEN ...
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bioRxiv - Microbiology 2021Quote: ... One step RT-qPCR for genomic viral RNA was performed using specific primer-probe sets and the QuantiFast Probe RT-PCR +ROX Vial Kit (QIAGEN), in the Rotor-Gene Q (QIAGEN ...
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bioRxiv - Cancer Biology 2020Quote: ... Expression of miR-181a was validated by q-PCR using primers (cat # MS00008827, Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... 0.6 µM each XBP1 specific primers and one-step RT-PCR (QIAGEN OneStep RT-PCR, 210212). The PCR products were analysed by 2% agarose gel ...
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bioRxiv - Cancer Biology 2021Quote: ... Mir-103 (Qiagen primer Cat. No. YP00204306) was used as reference miRNA ...
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bioRxiv - Molecular Biology 2023Quote: ... with primers targeting miR-451a (QIAGEN, 3624799) and U6 snRNA (v2 ...
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bioRxiv - Cell Biology 2023Quote: ... Two different kits were used for this method depending on the primer set: Rotor-Gene SYBR® Green RT-PCR Kit (Qiagen, 204174) and SensiFAST™ SYBR® No-ROX One-Step Kit (BioLine ...
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bioRxiv - Immunology 2020Quote: ... the miScript Universal Primer was used alongside miR specific miScript primer assays (miR-206 cat. no. MS00001869 and U6 cat. no. MS00033740; Qiagen).
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bioRxiv - Genetics 2024Quote: ... and the following miRCURY LNA miRNA PCR primer sets (Qiagen, Hilden, Germany): dme-miR-210-3p (YP02104327) ...
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bioRxiv - Pathology 2021Quote: ... using QuantiTect SYBR Green RT-PCR Kit and QuantiTect Primers (Qiagen). Three housekeeping gene mRNAs (GAPDH ...
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bioRxiv - Neuroscience 2021Quote: ... Relative-fold changes were normalized comparing exosomal micro RNA reference gene miR-30c-5p using the primer hsa-miR-30c-5p miRCURY LNA miRNA PCR Assay (Qiagen 339306, gene globe ID YP00204783). Relative fold changes of miR223-3p of fro/fro mice were calculated to +/fro mice by using the formula ...
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bioRxiv - Molecular Biology 2021Quote: ... with miRCURY LNA™ primers (YP00203907 for U6; YP00205637 for miR-10b, YP00204339 for miR-125a) (QIAGEN). For mRNA analysis ...
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bioRxiv - Plant Biology 2021Quote: ... Amplification of a single product of the expected size by a primer pair was first confirmed by RT-PCR using Qiagen® One-Step RT-PCR kit (Qiagen) followed by agarose gel analysis of the amplified products ...
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bioRxiv - Molecular Biology 2021Quote: ... The following primer sets from Qiagen were used ...
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bioRxiv - Immunology 2020Quote: ... Mature miR-511-3p-specific primers were obtained from Qiagen and relative expression was normalised to U6 (RNU6-2 ...
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bioRxiv - Genetics 2022Quote: ... 5 μl of the reverse transcribed products was amplified with the Vβ forward primers and Jβ reverse primer set pools (0.2 μM each) using a Multiplex PCR Kit (206143, QIAGEN, Germany). The following PCR program was used ...
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bioRxiv - Genetics 2022Quote: ... Routine qPCR for miR detection and validation (see Supplementary Table 1 for miR sequences) was performed with the miScript PCR control set (catalog number 218380; Qiagen, Germany). The miScript™ miRNA PCR Array Human Serum & Plasma 384HC (Cat No ...
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bioRxiv - Developmental Biology 2021Quote: ... using primers amplifying miR let7 (Qiagen, MS00005866; let-7g Qiagen, MS00010983).
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bioRxiv - Developmental Biology 2021Quote: ... using primers amplifying miR let7 (Qiagen, MS00005866; let-7g Qiagen, MS00010983).
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bioRxiv - Microbiology 2019Quote: ... Cementing PCR was performed using primer set 4438/441 and cleaned up using a QIAquick PCR Purification Kit (Qiagen) and transformed into DK1042.
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bioRxiv - Neuroscience 2021Quote: ... Validated primer sets were obtained from Qiagen.
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bioRxiv - Neuroscience 2024Quote: ... Validated QuantiTect primer sets obtained from (Qiagen) for IL-1α (QT00001127) ...
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bioRxiv - Microbiology 2020Quote: ... The triplicate PCR amplicons from each sample-primer set combination were then pooled and purified using the QIAquick PCR purification kit (Qiagen), with a slightly altered protocol in which the final elution was performed on nuclease-free water pre-heated to 55°C and incubated for 5 min at 55° C before the final centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... All reactions were set up in triplicate using a One-Step RT-PCR kit (Qiagen, UK) as per the following run conditions ...
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bioRxiv - Cancer Biology 2022Quote: ... according to manufacturer’s protocol (MiScript Primer assays and II RT kit for cDNA synthesis and MiScript SYBR Green PCR Kit for RT-qPCR, 218161, Qiagen) from which the recovery of cel-miR-39 spike-in control was confirmed.
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bioRxiv - Developmental Biology 2020Quote: ... and quantitative RT-PCR was performed using SYBR-based primers (Supplemental Table 1) and RT SYBR Green qPCR Mastermix (Qiagen, #330509) on a CFX96 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... and miR-42-3p were obtained from the miScript Primer Assays kit (Qiagen). miR-47-3p was used as the endogenous control ...
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bioRxiv - Developmental Biology 2019Quote: ... Predesigned primers for miR-126 and U6 were purchased from Qiagen (cat # 218300).
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bioRxiv - Developmental Biology 2020Quote: ... RT-PCR was performed using OneStep RT-PCR Kit (QIAGEN) using the following primers ...
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bioRxiv - Immunology 2023Quote: ... was performed in duplicates using predesigned primer sets (Quantitect Primer Assays, Qiagen, Germantown, MD). Relative mRNA expression between groups was analyzed by the delta-delta Ct method and normalized to housekeeping genes ...
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bioRxiv - Cell Biology 2019Quote: ... qPCR was performed on a LightCycler 96 Real-Time PCR System using SYBR Green RT-PCR with the following Quantitec primers from Qiagen; IL-6 (QT0009887) ...
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bioRxiv - Immunology 2023Quote: ... cDNAs were synthesized using High Capacity RNA-to-cDNA kit (ThermoFischer Scientific) and quantitative RT-PCR were carried out using specific primers (Table S2) and SYBR Green PCR Master Mix (Qiagen). PCR amplification of Gapdh was performed to control for sample loading and normalization between samples ...
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bioRxiv - Microbiology 2020Quote: ... CDNA fragments were amplified with strain-specific primers using the one step RT-PCR kit (Qiagen). Sequencing was performed by Macrogen Europe ...
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bioRxiv - Neuroscience 2020Quote: ... 1 μM barcode LNA RT primer (Qiagen), 1U/μL RiboLock RNase inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... miR-29b and miR-29c (Qiagen), using Lipofectamine 2000 transfection reagent (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... RT-PCR was performed using the OneStep RT-PCR kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... RT-PCR was performed using One Step RT-PCR kits (Qiagen) and a mixture of TRAV- ...
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bioRxiv - Plant Biology 2024Quote: ... RT-PCR was conducted using a OneStep RT-PCR kit (Qiagen) with the following primer pair ...
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bioRxiv - Biochemistry 2020Quote: ... Reverse ttranscription (RT) for miRs was completed with 300 ng of total RNA using a miScript II RT Kit (Qiagen) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: RT-qPCR was performed with 0.4 μM primers and using the QuantiTect SYBR Green PCR mix (Qiagen). Primer sequences are indicated in Table 1 ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative RT-PCR was performed using QuantiTect Probe RT-PCR Kit (Qiagen) in a StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... RT-PCR reactions were prepared using QuantiTect Probe RT-PCR Kit (Qiagen), with final concentrations of primers and probe used were 400 nM and 200 nM respectively ...
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bioRxiv - Microbiology 2020Quote: ... rt-PCR was conducted with a OneStep RT-PCR kit (Qiagen Canada) by following the manufacturer’s instructions.