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Citations for Qiagen :
1 - 50 of 187 citations for cleaved Caspase 9 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and miR-9/9*-124 + shPTBP2 using TRIzol Reagent in combination with RNeasy mini kit (Qiagen, 74104). RNA quality (RIN > 9.6 ...
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bioRxiv - Microbiology 2020Quote: ... and a plasmid expressing VSV-G or HIVKB9 envelope glycoproteins were cotransfected at the mass ratio of 9:1 (9 Hi.fate / 1 Env) using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: We extracted RNA from 35 pituitaries and 35 gonads (17 Independents, 9 Satellites, 9 Faeders) using the RNeasy Minikit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... and chestnut oak with FUSE (n = 9) and conventional extraction methods (n = 9) using a lysis buffer and purified using silica columns (Qiagen DNeasy Plant Kit Qiagen Inc ...
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bioRxiv - Microbiology 2022Quote: ... coli strains containing expression plasmid pQE-9 (Qiagen), pQE9-ftsA ...
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bioRxiv - Microbiology 2023Quote: ... and the CLC Genomics Workbench 9 software (Qiagen) for analysis ...
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bioRxiv - Immunology 2023Quote: ... Samples were diluted 9:1 (elution buffer (Qiagen):cDNA ...
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bioRxiv - Biophysics 2021Quote: ... Cleaved protein was further purified by reloading into Ni-NTA resin (QIAGEN). The flow through was collected ...
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bioRxiv - Immunology 2022Quote: ... The cleaved proteins were passed over a 1mL Ni-NTA agarose (Qiagen) gravity column to remove TEV protease ...
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bioRxiv - Cell Biology 2022Quote: ... mAb anti-His6 (34650; IF: 1/500) from Qiagen, mAb anti-GAPDH (GTX28245 ...
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bioRxiv - Microbiology 2020Quote: ... and 9 dpi with a RNeasy Mini Kit (Qiagen) with DNase treatment ...
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bioRxiv - Biochemistry 2019Quote: ... The cleaved tag and protease were removed by Ni-NTA affinity chromatography (Qiagen) and CLOCK PAS-AB was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The cleaved protein was passed over a 1 mL Ni-NTA agarose (Qiagen) gravity column to remove TEV protease ...
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bioRxiv - Biophysics 2020Quote: ... Cleaved samples were applied to gravity flow columns packed with Ni-NTA Superflow (QIAGEN) and flow-through was collected ...
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bioRxiv - Microbiology 2021Quote: ... 6 and 9 employing the RNeasy plant mini kit (Qiagen). RNA was quantified using Nanodrop (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... Recombinant plasmids were then isolated (MiniPrep, Qiagen) and sequenced (DNA Sequencing Facility ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 647-conjugated Penta-His mAb was purchased from Qiagen, Germantown ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was treated with Penta-His MAb (Qiagen, Germantown, MD) followed by HRP-conjugated goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Combined digests were extracted with 9 × volumes of PB buffer (QIAGEN) and DNA was purified with MinElute columns on a QIAvac 24 Plus vacuum manifold system (QIAGEN).
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 M Tris/Bicine pH 8.5 with 9 µL of JCSG+ (Qiagen) condition #29 containing 0.8 M sodium phosphate monobasic monohydrate ...
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bioRxiv - Biophysics 2019Quote: ... Anti-tetra-His-tag mouse monoclonal antibody (mAb) IgG1 was purchased from Qiagen Com ...
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bioRxiv - Plant Biology 2021Quote: ... recombinant protein and purified using Ni-NTA resin (Qiagen). Polyclonal antibodies were raised in mice as described in 1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Recombinant protein was purified using Ni-NTA agarose (Qiagen). Rabbit polyclonal antibodies were generated by Covance ...
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bioRxiv - Microbiology 2019Quote: ... 6 and 9 hours of treatment using RNeasy Plus Mini Kit (Qiagen, 74134), and was reverse-transcribed to cDNA using Transcriptor First Strand cDNA Synthesis Kit with random primers (Roche ...
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bioRxiv - Immunology 2020Quote: ... and the cleaved His6-MBP tag was removed by passing the protein through an amylose resin column (Qiagen). The flow-through fraction containing the tag-free protein was further purified using a Superdex 200 (10/300 GL ...
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bioRxiv - Biochemistry 2023Quote: ... Cleaved CK1δ ΔC was further purified away from His-GST and His-TEV using Ni-NTA resin (Qiagen) and subsequent size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Developmental Biology 2020Quote: ... recombinant proteins were purified using Ni-NTA Agarose (Qiagen 30210). Buffer was exchanged by dialysis to PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Recombinant proteins were purified using Ni-NTA affinity resin (Qiagen) according to standard protocols (21) ...
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bioRxiv - Biophysics 2023Quote: ... The recombinant proteins were purified using nickel affinity chromatography (Qiagen). As the final purification step ...
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bioRxiv - Cancer Biology 2023Quote: ... Recombinant proteins were then purified by Ni-NTA-agarose (Qiagen) affinity chromatography ...
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bioRxiv - Microbiology 2019Quote: ... Consequent fastq output files were examined using CLC Genomics Workbench 9 (Qiagen Aarhus, Denmark).
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bioRxiv - Evolutionary Biology 2020Quote: ... Digests were combined and DNA was extracted with 9 × volumes of PB buffer (QIAGEN) or a 3:2 mixture of QG buffer (QIAGEN ...
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bioRxiv - Neuroscience 2022Quote: ... and 15μL of 100ng/mL d3-CBD (Qiagen TissueLyser II (30 hz, 9 min)) ...
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bioRxiv - Molecular Biology 2023Quote: ... After purification of AtH2B.9 using nickel-nitrilotriacetic acid agarose (Ni-NTA) resin (QIAGEN), the His6-tag portion was removed by TEV protease (30 µg/mg of His6-H2B.9) ...
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bioRxiv - Plant Biology 2023Quote: ... The His and GST tag were cleaved by the Tev protease and separated from AtPAXXL228E using Ni-NTA (Qiagen). AtPAXXL228E was loaded onto a HiTrap Q HP column equilibrated with 20 mM Tris pH7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified with Ni-NTA chromatography (Ni2+-nitrilotriacetate, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Recombinant plasmids were purified with EndoFree Plasmid Maxi Kit (12362, QIAGEN). Oligonucleotide sequences and further details are provided in Supplementary Table 1 ...
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bioRxiv - Microbiology 2022Quote: ... recombinant protein was initially purified by nickel affinity chromatography (Qiagen, Holland) and subsequent size exclusion using Superdex 200 increase (GE Healthcare ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant 6xHis-Uhrf1 was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 40,920 cm-1 M-1) ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant 6xHis-Swi6 was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 45,330 cm-1 M-1) ...
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bioRxiv - Immunology 2020Quote: ... Recombinant HCMVs were reconstituted from HCMV BAC DNA by Superfect (Qiagen) transfection into permissive MRC-5 cells.
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bioRxiv - Immunology 2023Quote: ... Recombinant protein was purified by binding to Ni-NTA beads (Qiagen) and eluted in the same buffer supplemented with 300 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were purified with Ni2+-nitrilotriacetic acid agarose beads (Qiagen) and desalted with Bio-Gel P-6DG gel (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... 9 mL 1M Tris HCl pH 7.5, 9 mL 0.5M EDTA pH 8.0, 11.25 mL 10% SDS, 22.5 mL Qiagen lysis reagent ...
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bioRxiv - Genomics 2021Quote: 72 µl Proteinase K digestion solution (63 uL Qiagen PKD buffer, 9 uL Proteinase K) was injected into the flow cell and incubated at 55°C for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... The his-tagged 3C protease was removed by flowing and washing off cleaved transporter protein over two 100 µl beds of Ni-NTA Superflow resin in tandem (Qiagen). Eluent was concentrated using 100 kDa cut-off Amicon Ultra filters (Millipore-Sigma).
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bioRxiv - Biophysics 2021Quote: ... PNGase F and the cleaved 10 × His tag were removed by passing the sample through Ni-NTA superflow resin (QIAGEN). The receptor was concentrated to 20–30 mg/ml with a 100 kDa cut-off concentrator (Millipore) ...
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bioRxiv - Cancer Biology 2022Quote: ... The cleaved fragments were released by incubating the tube for 30□min at 37□°C and then purified by Qiagen MinElute PCR Purification Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved sP36 protein was afterwards purified by a second step of affinity using Ni-NTA agarose (Qiagen, Hilden, Germany) in phosphate buffer A.
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNAs from the pool and var2-9 were extracted with the DNeasy Plant kit (Qiagen). One μg of genomic DNA was used for library construction with the Truseq DNA PCR-free kit (Illumina ...