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Citations for Qiagen :
1 - 50 of 2627 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Muscles were homogenized in a similar method to Western blot preparation with the exception of RLT buffer supplied in the RNeasy® Mini Kit (Qiagen 74104) supplemented with 1% β-mercaptoethanol (Sigma M6250 ...
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bioRxiv - Molecular Biology 2020Quote: ... Collected 10uL for western blot of fractions and added 1mL of QIAzol (Qiagen) to remaining chromatin and incubated at 50°C for 10 min to solubilize chromatin ...
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bioRxiv - Microbiology 2020Quote: ... Western blot analysis was performed using the Penta His HRP conjugate kit (Qiagen). To check for equal loading ...
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bioRxiv - Developmental Biology 2023Quote: ... the precipitates were subjected to Western blot analysis with anti-penta-His (1:2000, Qiagen) and anti-FLAG M2 (1:4000 ...
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bioRxiv - Genetics 2021Quote: ... The western blot analysis was performed according to the QIAexpress detection kit and the protocol (Qiagen) with the following modifications ...
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bioRxiv - Neuroscience 2023Quote: ... GFP+ and GFP- nuclei were sorted using a BD AriaFACS III (University of Washington Pathology Flow Cytometry Core) into a PCR tube strip containing 3 µL of REPLI-g Advanced Single Cell Storage buffer (Qiagen). Whole genome amplification (WGA ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-His Western blots were performed using Penta-His mouse monoclonal IgG1 as primary antibody (Qiagen, #34660) at 1:2000 dilution ...
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bioRxiv - Microbiology 2019Quote: ... and Western blot analysis was performed as previously described (30) using a primary anti-his antibody (Qiagen) and a secondary Alexa Fluor 700 goat anti-mouse antibody ...
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bioRxiv - Pathology 2021Quote: ... the cells were harvested for western blot or for RNA isolation (Qiagen RNeasy kit, Qiagen, Germantown, MD). cDNA was synthesized using 500 ng RNA according to the iScript cDNA Synthesis kit protocol (Bio-Rad) ...
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bioRxiv - Pathology 2021Quote: ... the cells were harvested for western blot or for RNA isolation (Qiagen RNeasy kit, Qiagen, Germantown, MD). cDNA was synthesized using 500 ng RNA according to the iScript cDNA Synthesis kit protocol (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Cell Biology 2021Quote: Antibodies and their conditions of use are as follows: mouse anti-His (Western blot, 1:1000; 34660; Qiagen), rabbit anti-Brl1 (Western blot ...
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bioRxiv - Biochemistry 2022Quote: ... Expression levels were validated from the pWB plasmid by western blot using an anti-His HRP conjugate kit (Qiagen) (Fig ...
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bioRxiv - Molecular Biology 2019Quote: ... protein samples were processed further according to the Western Blot protocol and RNA was extracted using an RNAeasy Kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: ... Pulled-down fractions were analysed by SDS-PAGE or Western blot using rabbit antibodies against KtrB or mouse against His-tag (Qiagen).
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bioRxiv - Microbiology 2023Quote: ... and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000, Qiagen) as primary antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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bioRxiv - Microbiology 2019Quote: ... soluble and insoluble fractions were separated by centrifugation and analyzed by SDS-PAGE and western blot using anti-Strep (Qiagen, Hilden, Germany) and anti-His (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were incubated at 95°C for 5 minutes and spun down at 17K x g for 10 min to remove cell debris before analysis of raw supernatant was performed via SDS-PAGE using a 12% acrylamide-tris gel and subsequent overnight transfer to a Western blot PVDF membrane and visualization with an anti-His antibody (Qiagen Cat# 34440, RRID:AB_2714179).
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bioRxiv - Cell Biology 2019Quote: ... We then extracted RNA from the membrane strip by following manufacturer’s manual of the RNA extraction kit (RNeasy kit, cat# 74104, Qiagen) by adding 100ul of RLT+ β-mercapto-ethanol ...
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Biochemistry 2021Quote: ... analysed by western-blotting using Penta·His Antibody (QIAGEN), of wild-type OxlT measured on the same day of experiment ...
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bioRxiv - Molecular Biology 2021Quote: ... to Rotor-Gene strip reaction tubes (Starlab, 22143, Hamburg, Germany) and RT-qPCR analysis was performed using the Rotor-Gene Q device (Qiagen, 40724, Hilden, Germany). RNase free H2O (Merck-Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... containing buffer Buffer RLT (Qiagen) using Precellys 24 homogenizer ...
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bioRxiv - Microbiology 2023Quote: ... blots were incubated with mouse monoclonal RGS-His antibody (Qiagen) diluted 1:1,000 ...
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bioRxiv - Biochemistry 2021Quote: ... 700 µL buffer RLT (kit buffer, Qiagen) supplemented with 10 µL/mL β-mercaptoethanol was added to the sample and mixed vigorously before proceeding with the protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... The buffers used were: 1x PCR buffer (Qiagen; referred to as Taq buffer); 1x ThermoPol reaction buffer (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... containing 10uL of lysis buffer (TCL buffer, Qiagen 1031576 supplemented with 1% beta-mercaptoethanol) ...
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bioRxiv - Immunology 2020Quote: ... sorting directly into lysis buffer (RLT buffer (Qiagen)) for timepoint 0hrs ...
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bioRxiv - Microbiology 2023Quote: ... but instead of RW1 buffer RWT buffer (Qiagen) was used in order to isolate RNAs smaller than 200 nucleotides also ...
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bioRxiv - Microbiology 2024Quote: ... 180 μL of lysis buffer (ATL buffer; Qiagen) and 40 μL of proteinase K (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysis buffer was comprised of RLT Buffer (Qiagen) and 10% beta-mercaptoethanol ...
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bioRxiv - Plant Biology 2020Quote: ... 2.0 μL of PCR buffer (Qiagen 10x PCR Buffer), 1.0 μL of DMSO ...
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bioRxiv - Developmental Biology 2022Quote: ESCs were homogenized in RNA lysis buffer (RLT buffer, Qiagen) with 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... 5 mL erythrocyte lysis buffer (EL buffer, Qiagen, Hilden, Germany) was added and PMNs were centrifuged ...
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bioRxiv - Neuroscience 2024Quote: Cells were sorted into RNA lysis buffer (RLT buffer, Qiagen) and total RNA was isolated using the RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... RLT buffer (Qiagen) with 0.01% v/v 2-mercaptoethanol (Sigma ...
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bioRxiv - Immunology 2021Quote: ... RLT buffer (Qiagen) was then added to the pellet and RNA was extracted using an RNeasy Plus Mini kit (Qiagen ...
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bioRxiv - Synthetic Biology 2020Quote: ... Buffer PE (Qiagen), and DCC-5 columns (Zymo Research) ...
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bioRxiv - Genetics 2020Quote: ... elution buffer (Qiagen) and tip combs (Qiagen) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... PE buffer (Qiagen) was then used to wash the sample ...
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bioRxiv - Neuroscience 2023Quote: ... elution buffer (Qiagen) and tip combs (Qiagen) ...
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bioRxiv - Biochemistry 2021Quote: ... Blots were probed with antibodies against His6 (penta-His Qiagen catalog #34460; 1:10,000), FLAG2 (Sigma catalog #A8592 ...
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bioRxiv - Microbiology 2020Quote: ... 360μl tissue lysis buffer (ATL buffer, included in the Qiagen kit) was added to each tube and the sample was bead beaten four times at 5000 rpm for 18 seconds in a Precellys tissue homogeniser (Bertin ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA was eluted in high-salt buffer (Buffer QF) (QIAGEN). Using isopropanol precipitation ...
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bioRxiv - Molecular Biology 2022Quote: ... then resuspended in RNA lysis buffer (RLT buffer, Qiagen, Venlo, Netherlands). In vitro differentiated adipocytes were washed twice with cold 1X PBS and collected in RLT buffer ...
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bioRxiv - Physiology 2019Quote: ... in 40 μl Binding Buffer (Pyromark binding buffer, Qiagen, Manchester UK) and 25 μl Milli-Q-water ...
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bioRxiv - Neuroscience 2020Quote: ... resuspended in PK buffer (PK buffer from RNeasy FFPE Kit, Qiagen) and stored at −80°C until extraction was done ...
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bioRxiv - Immunology 2020Quote: ... and total RNA was isolated using lysis buffer (Buffer RLT; QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... the samples were purified with 500 µL of PB buffer and 650 µL of PE buffer and eluted in 30 µL of EB buffer (MinElute PCR Purification Kit, QIAGEN). As previously ...