Labshake search
Citations for Qiagen :
1 - 50 of 474 citations for Vertical Electrophoresis system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... and visualized on QIAxcel Capillary Electrophoresis System (Qiagen). Libraries were sequenced on a MiSeq run (Illumina ...
-
bioRxiv - Microbiology 2019Quote: ... amplified samples were subjected to gel electrophoresis with QIAxcel advance system (QIAGEN, USA) (16) ...
-
bioRxiv - Biochemistry 2021Quote: ... followed by capillary electrophoresis of the PCR products using a QIAxcel Advanced System (QIAGEN).
-
bioRxiv - Molecular Biology 2020Quote: ... the capillary electrophoresis system QIAxcel Advanced and the software QIAxcel ScreenGel (version 1.4.0, Qiagen) with the method AM320 and 30 s injection time were used ...
-
bioRxiv - Molecular Biology 2020Quote: ... All PCR products were examined using the QIAxcel® capillary electrophoresis system 100 (Qiagen).
-
bioRxiv - Molecular Biology 2023Quote: ... All PCR products were examined using the QIAxcel® capillary electrophoresis system 100 (Qiagen). Statistical analyses were performed using GraphPad Prism 9.5.0.
-
bioRxiv - Molecular Biology 2023Quote: ... Reactions were analysed and quantified using the QIAxcel® capillary electrophoresis system 100 (Qiagen).
-
Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... quality control of isolated RNA was performed on a QIAxcel capillary electrophoresis system (Qiagen, Hilden, Germany). Integrity of RNA was proven by reporting the 28S/18S ratio and the RIS number (RNA Integrity Score ...
-
bioRxiv - Molecular Biology 2024Quote: ... Resultant amplicons were checked for predicted amplicon sizes using a QIAxcel electrophoresis system (QIAGEN, Maryland, USA). All reactions were performed in triplicate with each plate containing an equal number of samples from each group (N=6) ...
-
bioRxiv - Genomics 2022Quote: ... and Qiaxcel capillary electrophoresis (Qiagen). RNA integrity number (RIN ...
-
bioRxiv - Molecular Biology 2020Quote: ... The PCR products were also run on a QIAxcel capillary gel electrophoresis system according to the manufacturer’s instructions (Qiagen, Germany) and fragments were sized and scored using QIAxcel ScreenGel software (Qiagen ...
-
bioRxiv - Genetics 2019Quote: ... purified using an E-gel electrophoresis system (Agarose 2%) followed by column purification with a MinElute PCR Purification Kit (Qiagen). In order to introduce the doped sequence in the full-length TDP-43 sequence the purified oligonucleotide was cloned into 100 ug of linearized pRS416 Gal TDP-43 by a Gibson approach (Supplementary Table 1 ...
-
bioRxiv - Genetics 2024Quote: ... and proteinase K was inactivated at 95°C for five minutes.39 PCR was performed on isolated genomic DNA followed by analysis on a QIAxcel Advanced System instrument using capillary electrophoresis (Qiagen). Primer3plus45 was used to design primers and oligonucleotides obtained from IDT (Supplementary Table 1).
-
bioRxiv - Microbiology 2020Quote: ... and amplicon length was measured by comparison against QX DNA Size Marker using the Qiaxcel Automatic electrophoresis system (QIAGEN, Hilden, Germany). Samples were sequenced by the Center for Translational Genomics and BioInformatics (Milan ...
-
bioRxiv - Microbiology 2022Quote: ... triplicate reactions were re-pooled using the epMotion and DNA concentrations were quantified using a QIAxcel Advanced capillary electrophoresis system (Qiagen, Hilden, Germany). Samples with concentrations > 0.5 ng/µl were pooled using equal DNA masses to create the final sequencing libraries ...
-
bioRxiv - Microbiology 2021Quote: ... triplicate reactions were re-pooled using the epMotion and DNA concentrations were quantified using a QIAxcel Advanced capillary electrophoresis system (QIAgen, Hilden, Germany). Samples that had concentrations > 0.5 ng/μl were pooled using equal weights of DNA to create the final sequencing libraries ...
-
bioRxiv - Microbiology 2023Quote: ... Triplicate PCR reactions were then re-pooled using the epMotion 5075 and DNA concentrations were quantified using a QIAxcel Advanced capillary electrophoresis system (QIAgen, Hilden, Germany). An equal mass of DNA (up to a maximum volume of 12 μL ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Junction PCRs were analyzed via QIAxcel Capillary Electrophoresis (Qiagen) and visualized with QIAxcel ScreenGel Software (v1.5.0.16 ...
-
HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... detected and quantified by capillary gel electrophoresis (QIAxcel, QIAgen) (Supplementary Table 7).
-
bioRxiv - Genomics 2023Quote: ... PCR products were visualized via QIAxcel capillary electrophoresis (Qiagen) and then purified and normalized via an NGS normalization 96 well kit (Norgen BioTex Corp ...
-
bioRxiv - Neuroscience 2024Quote: ... gel electrophoresis and subsequent QIAquick™ gel extraction (Qiagen). Linear fragments were then re-ligated using the Rapid DNA Dephosphorylation and Ligation Kit (Roche ...
-
bioRxiv - Neuroscience 2023Quote: ... purified by agarose gel electrophoresis followed by QIAEX II (Qiagen), and injected into pronuclei of fertilized one-cell eggs from FVB at the Stanford Transgenic Facility ...
-
bioRxiv - Developmental Biology 2021Quote: ... After validating library size distribution using the QIAxcel capillary electrophoresis (Qiagen), the libraries were further purified using the SPRIselect reagent to remove large DNA molecules (a right-side size selection with 0.55X followed by 1.5X ratios of beads ...
-
bioRxiv - Neuroscience 2020Quote: ... and extracted from electrophoresis gel using QIAquick Gel Extraction Kit (Qiagen 28704) according to manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... Product purity was assessed via capillary electrophoresis on a QIAxcel instrument (Qiagen). The NEBNext Ultra II DNA Library Prep kit (NEB ...
-
bioRxiv - Genetics 2023Quote: ... PCR products were purified prior to quantification via capillary electrophoresis (Qiagen QIAxcel), normalization ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Amplicons were isolated via gel-electrophoresis and gel-extraction (Qiagen Gel Extraction Kit), and subsequently used as template in enrichment PCR using the same primers ...
-
bioRxiv - Microbiology 2022Quote: ... PCR products were separated by electrophoresis on a 1% agarose gel (Qiagen, USA). The bands corresponding to full-length gene segments were excised and the DNA was recovered using QIAquick gel extraction kit (Qiagen ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR products were analysed with multi-capillary electrophoresis using QIAxcel (Qiagen; Hilden, Germany). Some of the PCR products performed inadequately in terms of amplification success and PCRs were repeated in 25 μl volumes containing 2 μl ultrapure H2O ...
-
bioRxiv - Genomics 2023Quote: ... The product purity was assessed via capillary electrophoresis on a QIAxcel instrument (Qiagen) and quantified by spectrophotometry (NanoDrop®) ...
-
bioRxiv - Bioengineering 2023Quote: ... PCR-2 products were pooled based on concentrations from capillary electrophoresis (QIAxcel, Qiagen). Final libraries were quantified by Qubit dsDNA High Sensitivity assay (ThermoFisher ...
-
bioRxiv - Cell Biology 2021Quote: ... separated by agarose gel electrophoresis and purified using QiaEx II gel extraction resin (Qiagen). Log-phase promastigotes were transfected with 1-5 μg and cloned by limiting dilution ...
-
bioRxiv - Biochemistry 2022Quote: ... Cleavage fragments were purified using paramagnetic beads and quantified via QIAxcel capillary electrophoresis (Qiagen). The relative abundances of substrate and products were analyzed using QIAxcel ScreenGel Software (v1.5.0.16 ...
-
bioRxiv - Biochemistry 2022Quote: ... The PCR products were analyzed through capillary gel electrophoresis with the QIAxel instrument (Qiagen). The cPCR-amplified scFv gene was then digested with BstNI endonuclease to obtain and compare the band patterning of each scFv amplified gene ...
-
bioRxiv - Neuroscience 2023Quote: ... PCR products were separated by capillary electrophoresis Qiaxcel® DNA High Resolution Kit (Qiagen).
-
bioRxiv - Neuroscience 2023Quote: ... Both vectors and PCR fragments were purified using gel electrophoresis and gel extraction (Qiagen) and were ligated using Gibson assembly (NEB) ...
-
bioRxiv - Neuroscience 2019Quote: ... PCR products were confirmed by gel electrophoresis and purified with MinElute PCR Purification Kit (Qiagen) prior to sequencing (Retrogen) ...
-
bioRxiv - Cell Biology 2019Quote: ... The fragments were purified after agarose gel electrophoresis with the QiaQuick Gel Extraction Kit (QIAGEN), cloned in pRACE vector as per kit instructions and transformed into Stellar component cells (Clontech ...
-
bioRxiv - Biochemistry 2022Quote: ... separated by agarose gel electrophoresis and DNA was recovered with QIAquick Gel Extraction Kit (Qiagen), according to supplier instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... PCR products were then purified by gel electrophoresis using the QIAquick Gel Extraction Kit (Qiagen). The Sanger reaction and electrophoresis for the forward and reverse sequences of each purified amplicon was performed by the CCR Genomics Core ...
-
bioRxiv - Synthetic Biology 2021Quote: ... PCR products were gel purified by agarose gel electrophoresis and isolated applying QIAquick columns (Qiagen), followed by in-vitro transcription applying the Megashortscript kit (Ambion) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The library was size selected by agarose gel electrophoresis and column purified (Qiagen, MinElute, #28606). Single-end sequencing was performed to an average of 30 million reads per sample using Illumina NovaSeq 6000 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The linear constructs were purified via agarose gel electrophoresis and QIAquick Gel Extraction Kit (Qiagen). Insert oligonucleotides were designed based on Avana-4 library single guide RNA (sgRNA ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The digested fragment was purified by gel electrophoresis and the QIAquick gel extraction kit (Qiagen) being careful to not expose the DNA to the fluorescent light for more time than was necessary and eluted in 20 µl of 1mM Tris-HCl pH 8.0.
-
bioRxiv - Neuroscience 2023Quote: ... The generated amplicon was purified after gel electrophoresis using the QIAquick Gel Extraction Kit (Qiagen) and served as template for in vitro transcription with MEGAshortscript T7 Transcription Kit (Ambion) ...
-
bioRxiv - Immunology 2023Quote: ... PCR products were analysed by gel electrophoresis and purified using QIAquick PCR purification kit (Qiagen). 100 ng purified DNA each from WT and sgRNA treated samples were denatured at 95°C for 5 min in 1× NEB 2 buffer and hybridized following the temperature profile 95 to 85°C at 2°C/s and 85 to 25°C at 0.1°C/s ...
-
bioRxiv - Cell Biology 2022Quote: ... RNA purity and integrity were checked by microfluidic electrophoresis using the Bioanalyzer (Qiagen, Hilden Germany). Following the qualtity control procedure ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Extracted DNA was quality and quantity screened using a QIAxpert microfluidics electrophoresis device (Qiagen, Valencia CA).
-
bioRxiv - Neuroscience 2022Quote: ... The PCR product was purified through with the QIAquick gel extraction kit after gel electrophoresis (QIAGEN). The PCR product was subcloned into a pENTR 5’-TOPO vector (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... separated by agarose gel electrophoresis and purified with the QIAquick Gel Extraction Kit (Qiagen, Aarhus, Denmark). Doublestranded DNA fragments (gBlocks ...