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Citations for Qiagen :
1 - 32 of 32 citations for Trypan Blue Dyes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... 0.01% bromophenol blue) or RLT (Qiagen) for proteome-phosphoproteome-ubiquitinome ...
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bioRxiv - Developmental Biology 2023Quote: ... with the carboxy-X-rhodamine (CXR) Dye and Rotor-Gen-Q (Qiagen) detection system ...
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bioRxiv - Bioengineering 2020Quote: ... SYBR Green dye was used to target synthesized cDNA (Quantifast SYBR Green I, Qiagen). Real-time PCR was then performed (7500 Real Time PCR system ...
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bioRxiv - Bioengineering 2020Quote: ... SYBR Green dye was used to target synthesized cDNA (Quantifast SYBR Green I, Qiagen). Real time PCR was then performed (7500 Real Time PCR system ...
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bioRxiv - Biophysics 2023Quote: ... All constructed plasmids were prepared from XL1-Blue cells using the QIAprep Spin Miniprep Kit (QIAGEN). Protein-coding sequences were verified by Sanger sequencing at UC-Berkeley DNA Sequencing Facility ...
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bioRxiv - Physiology 2021Quote: ... 20 μL reactions consisted of 1×QuantiFAST reaction mix containing ROX reference dye (Qiagen, Germany), 0.66 µM of forward and reverse primers ...
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bioRxiv - Genomics 2021Quote: ... The DNA samples were quantified using the Qubit 3.0 and dsDNA high sensitivity dye (Qiagen). Bacterial load was quantified using real-time PCR based on 16S rRNA gene for eubacteria (62 ...
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bioRxiv - Genomics 2021Quote: ... The DNA samples were quantified using the Qubit 3.0 and dsDNA high sensitivity dye (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... 20 μL reactions consisted of 1×QuantiFAST reaction mix containing ROX reference dye (Qiagen, Germany), 0.66 µM of forward and reverse primers ...
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bioRxiv - Cell Biology 2019Quote: ... Cy3-labeled miR-430 was separated from unconjugated dyes using a QIAquick Nucleotide Removal Kit (QIAGEN).
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bioRxiv - Genomics 2023Quote: ... with each 20 μL reaction consisting of 1× QuantiFAST reaction mix containing ROX reference dye (Qiagen), 0.66 µM forward and reverse primers ...
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bioRxiv - Microbiology 2023Quote: ... and the dye terminators from the reaction were cleared with DyeEx 2.0 Spin Kit (Qiagen, Germany). After post-purification ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transformed into XL1-Blue competent cells and plasmid DNA extracted using QIAprep Spin Miniprep Kit (Qiagen). The BUB1 K821R and BUB1C D946N alleles were generated by single-base substitution using site-directed mutagenesis (Strategene ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were amplified in Escherichia coli (XL-Blue) extracted by alkaline lysis and purified by column separation (Qiagen). The integrity was confirmed by gel electrophoresis in 0.8% agarose ...
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bioRxiv - Neuroscience 2021Quote: ... all libraries were quantified using the highly sensitive fluorescent dye-based Qubit ds DNA Assay Kit (Qiagen). Whole RNA library preparation was performed using the TruSeq stranded mRNA HT technology (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... All recombinant vectors were grown in XL1-Blue competent cells and extracted using QIAprep Spin Miniprep kit (Qiagen, Germany) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... or Taqman (qPCRBIO Probe Blue Mix Lo-ROX; PB20.25-05, qPCRBIO) and using the Rotor-Gene Q PCR system (Qiagen, Germany).
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bioRxiv - Molecular Biology 2024Quote: ... coli XL1-blue strain was transformed and plasmids were isolated from bacterial cultures using the Plasmid Midi Kit (Qiagen, Germany). Sequencing of the three NS1 plasmids was performed by Eurofins (Germany).
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bioRxiv - Cancer Biology 2019Quote: ... by excision from an agarose gel (using SYBR-safe stain and blue LED illumination) with purification by QIAquick Gel Extraction Kit (Qiagen 28704). PCR product from pre-treatment cell sample was processed separately rather than pooled with others ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmid DNA was extracted from 3-9 positive colonies where possible after blue-white screening using a QIAprep® Spin Miniprep Kit (Qiagen) and sequenced using the standard T7 primer at Eurofins Genomics ...
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bioRxiv - Genetics 2023Quote: ... Samples were amplified using RT2 SYBR® Green qPCR Mastermix with ROX (carboxy-X-rhodamine) passive reference dye from QIAGEN. The real-time PCR data were normalized by ROX passive reference ...
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bioRxiv - Systems Biology 2022Quote: ... The 1,606 bp fragment was cut under UV-free blue light and purified using the QIAquick Gel Extraction Kit (QIAGEN cat. no. 28704). The vector backbone and KRAB-MeCP2-P2A-BSD cassette were ligated by the following T4 ligase reaction ...
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bioRxiv - Systems Biology 2022Quote: ... The 10,992 bp was cut under UV-free blue light and purified using the QIAquick Gel Extraction Kit (QIAGEN cat. no. 28704). The truncated-dCas9-KRAB-MeCP2 cassette was synthesized by Integrated DNA Technology as 2,450 bp dsDNA fragments with a FspAI restriction site on 5’ end and a PmeI restriction site on 3’ end ...
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bioRxiv - Systems Biology 2022Quote: ... The 8,361 bp fragment was cut under UV-free blue light and purified using the QIAquick Gel Extraction Kit (QIAGEN cat. no. 28704).
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bioRxiv - Systems Biology 2022Quote: ... The 8,361 bp fragment was cut under UV-free blue light and purified using the QIAquick Gel Extraction Kit (QIAGEN cat. no. 28704).
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bioRxiv - Systems Biology 2022Quote: ... The 2,432 bp fragment was cut under UV-free blue light and purified using the QIAquick Gel Extraction Kit (QIAGEN cat. no. 28704). The vector backbone and truncated-dCas9-KRAB-MeCP2 cassette were ligated by the following T4 ligase reaction ...
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bioRxiv - Systems Biology 2022Quote: ... The 9,766 bp fragment was cut under UV-free blue light and purified using the QIAquick Gel Extraction Kit (QIAGEN cat. no. 28704). The trcrRNA-CaptureSeq-5’LTR(truncated ...
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bioRxiv - Systems Biology 2022Quote: ... The 13,792 bp fragment was cut under UV-free blue light and purified using the QIAquick Gel Extraction Kit (QIAGEN cat. no. 28704). The KRAB-MeCP2-P2A-BSD cassette was synthesized by Integrated DNA Technology as 1,634 bp dsDNA fragments with a BamHI restriction site on 5’ end and a SbfI restriction site on 3’ end ...
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bioRxiv - Systems Biology 2022Quote: ... The 74 bp fragment was cut under UV-free blue light and purified using the QIAquick Gel Extraction Kit (QIAGEN cat. no. 28704).
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bioRxiv - Microbiology 2019Quote: ... Proteins were then stained by Coomassie-blue or immunodetected as described before [26] using primary polyclonal antibodies directed against His6 epitope-tag (Penta His, Qiagen, dilution 1:1000), V5 epitope-tag (Bethyl Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspend the cells in 10 ml of suspension buffer (10 mM EDTA pH8.0, 150 mM NaCl, 1% glycerol, Lysis blue (1×, from QIAGEN Plasmid Plus Midi Kit), RNase A (0.55 mg/ml) ...
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bioRxiv - Immunology 2022Quote: ... Antigen specific B cells were gated with CD19+/IgM-/IgD-/Ghost dye-/PE+/BV605+ and sorted in catch buffer B (Qiagen TCL Buffer + 1% beta mercaptoethanol) by one cell per well in a 96 well plate ...