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Citations for Qiagen :
1 - 44 of 44 citations for Transglutaminase Substrate biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... using biotinylated His5-antibody (Qiagen), as previously described (Asbury et al. ...
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bioRxiv - Microbiology 2021Quote: For biotinylated RIPs HEK-293T cells were seeded and transfected with 20nM biotinylated LNA miR-29b or miR-30c (Qiagen) in combination with 2 μl Lipofectamine RNAi Max (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... were incubated with biotinylated anti-5His antibodies (Qiagen) and stored for up to 6 months with continuous rotation at 4°C in BRB80 (80 mM Pipes ...
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bioRxiv - Genetics 2022Quote: ... enzyme and substrate (Qiagen) and the optical plate is loaded to the machine ...
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bioRxiv - Biophysics 2019Quote: ... Biotinylated mouse anti-5xHis antibody was purchase from QIAGEN. Mouse anti Tubulin beta 3 antibody from BioRad (Hercules ...
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bioRxiv - Biophysics 2024Quote: ... were functionalized with biotinylated α-penta-His antibody (Qiagen, Hilden Germany or R&D Systems ...
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bioRxiv - Microbiology 2019Quote: ... A 10 nM solution of biotinylated penta-His antibody (Qiagen) was incubated for 10 min on the neutravidin-coated surface and excess unbound antibody removed (22 ...
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bioRxiv - Cell Biology 2021Quote: ... were functionalized with biotinylated anti-5His antibodies (Qiagen, Valencia CA) and stored with continuous rotation at 4°C in BRB80 (80 mM PIPES ...
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bioRxiv - Genomics 2022Quote: ... purification and the biotinylated cDNA (in 40 μl EB, Qiagen) was bound to 15 μl 1x SSPE washed Dynabeads™ M-270 Streptavidin beads (Thermo ...
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bioRxiv - Biophysics 2020Quote: ... functionalized with neutravidin and treated with biotinylated anti-hexahistidine monoclonal antibody (Qiagen) as described (15) ...
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bioRxiv - Biophysics 2020Quote: ... the surface was incubated with 1 nM biotinylated penta-His antibody (Qiagen) in buffer A for 10 min ...
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bioRxiv - Genomics 2022Quote: ... The biotinylated strand was purified using a PyroMark Q96 Vacuum Workstation (QIAGEN) then sequencing primers annealed in annealing buffer (20mMTris-acetate pH7.6 ...
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bioRxiv - Biophysics 2021Quote: ... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
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bioRxiv - Cancer Biology 2020Quote: ... Pyrosequencing of biotinylated PCR products was performed using PyroMark Q48 Advanced CpG reagents (Qiagen) on a Pyromark Q48 Autoprep apparatus (Qiagen ...
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bioRxiv - Genomics 2023Quote: ... Button valves were opened and biotinylated anti-pentaHis antibody (Qiagen, 1:4 dilution in HEPES) was flowed for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... together with 0.2 uM of each biotinylated primer and 0.25 units of HotStarTaq PlusDNA Polymerase (Qiagen). Refer to Supplementary Table 10 for primer sequences ...
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bioRxiv - Cancer Biology 2023Quote: ... Biotinylated RNA was eluted into 700μL Buffer RLT from the RNeasy MinElute Cleanup Kit (Qiagen, 74204) through two additions of 100 mM DTT (100μl ...
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bioRxiv - Biophysics 2023Quote: ... the glass was additionally treated with biotinylated anti-hexahistidine monoclonal antibody (Penta-His Biotin Conjugate; Qiagen) as in Duchi et al.23 and Dulin et al.31.
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bioRxiv - Developmental Biology 2021Quote: ... DNA or cDNA was amplified using the following biotinylated primers and subsequently sequenced using Q24 Pyromark (Qiagen).
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bioRxiv - Neuroscience 2020Quote: ... The biotinylated amplicons were verified with gel electrophoresis and extracted with the MinElute Gel Extraction Kit (Qiagen). Pyrosequencing was done using a Pyromark Q106 ID pyrosequencer with technical triplicates and CpG methylation levels were calculated using the Pyromark Q-CpG 1.0.9 software.
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bioRxiv - Genetics 2021Quote: ... Biotinylated RNA was released from beads using 100 mM DTT and cleaned using RNeasy MinElute Kit (QIAGEN 74204). Half of eluted RNA or 600 ng of depleted RNA was ligated to 5 µM of 50 adenylated ...
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bioRxiv - Genomics 2022Quote: ... and then PCR-amplified with biotinylated primers and pyrosequenced for allele quantification on a Pyromark Q24 system (Qiagen). The same PCR was done on no-reverse transcription control samples to confirm absence of genomic DNA contamination ...
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bioRxiv - Cancer Biology 2019Quote: ... The reverse transcription step exactly followed SMART-seq2 protocol with 5’-biotinylated TSO (Qiagen, primers see Table S9). The first round of 24-cycle PCR started with 10µl reverse transcription product ...
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bioRxiv - Developmental Biology 2023Quote: ... Plates were then placed at 4 0C before adding the reverse transcription mix containing 5’-biotinylated TSO (Qiagen). PCR products were cleaned up using 0.8:1 Ampure XP beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2019Quote: ... Biotinylated PCR products were mixed with streptavidin beads and converted to single strands using PyroMark Q96 Vacuum Workstation (Qiagen). Following annealing of sequencing primers ...
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bioRxiv - Genetics 2020Quote: ... Target regions were PCR-amplified in technical triplicates from bilsulphite-converted DNA using a biotinylated forward or reverse primer and HotStarTaq DNA Polymerase (QIAGEN). PCR conditions were as follows ...
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bioRxiv - Genomics 2020Quote: Bisulfite converted DNA was amplified with a region-specific biotinylated/unmodified DNA primer pair (Metabion; Supplemental Table S4) using the PyroMark PCR Kit (Qiagen) according to the manufactures instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Kinetochore proteins were conjugated to these beads using antibodies that recognize specific proteins or their tags: biotinylated anti-His-tag antibodies (6 µg ml−1, Qiagen), biotinylated anti-GFP antibodies (20 µg ml−1 ...
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bioRxiv - Developmental Biology 2020Quote: ... For allelic-skewing analysis the cDNA was PCR-amplified with biotinylated primers and sequenced using the Pyromark Q24 system (QIAGEN).
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bioRxiv - Genetics 2020Quote: ... 1μl of bisulfite converted DNA was used as template to amplify target genomic regions using specific primers (one biotinylated) with the PyroMark PCR kit (Qiagen, #978703), following the recommended conditions (annealing temperature 56°C) ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μl of each input and biotinylated RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen #74104) using the modified protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The relative abundances of substrate and products were analyzed using QIAxcel ScreenGel Software (v1.5.0.16, Qiagen) and plotted using GraphPad Prism 9 (v9.2.0).
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bioRxiv - Biophysics 2019Quote: ... the 69 nt gap DNA substrate was gel purified by QIAquick Gel Extraction Kit (Qiagen), as described.33 The final product consists of 69 nt ssDNA flanked with 441 bp and 235 bp dsDNA ...
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bioRxiv - Cell Biology 2020Quote: RNA FISH was performed using a FITC-labeled DNA oligo probe complementary to alpha satellite (Integrated DNA Technologies, Newark, NJ) or a biotinylated locked nucleic acid (LNA) oligo probe complementary to HSATII (QIAGEN, Hilden, Germany) (see Key Resources Table) ...
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bioRxiv - Genetics 2021Quote: ... we extracted DNA from the secondary substrates using the QIAamp DNA Investigator Kit (QIAGEN, Valencia, CA). The procedure from the QIAamp DNA Investigator Handbook for the Isolation of Total DNA from Body Fluid Stains was selected as it has a denim option to remove any inhibitors ...
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bioRxiv - Microbiology 2019Quote: Bat and substrate swabs were extracted using DNeasy Blood and Tissue extraction kits (Qiagen, Valencia, CA). The protocol was modified for fungal extractions to include lyticase during the lysis step in addition to the proteinase K and buffer ATL (Shuey ...
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bioRxiv - Cancer Biology 2021Quote: ... washed again and proteins were visualized by SuperSignal™ West Dura Extended Duration Substrate (Qiagen #34076)
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bioRxiv - Molecular Biology 2019Quote: ... The desired ~3 kb product was separated on a 1x TBE agarose gel and recovered by electroelution and ethanol precipitation (fluorescent DNA substrates) or with the QIAquick gel extraction kit (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from 4 g of soil from the identified segment of three 13C soil cores and from the 12C-control for each substrate/sample site combination using the DNeasy PowerMax Soil Kit (Qiagen). DNA was quantified fluorometrically using the Qubit dsDNA Broad-Range assay (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: Cells were cultured on a glass substrate and soft hydrogel for 3 days and total RNA was extracted using RNeasy mini kit (Qiagen). RNA quantity and purity were verified using 2200 TapeStation system (Agilent Technologies) ...
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bioRxiv - Genetics 2024Quote: ... and a negative control region within a regulatory region of the estrogen receptor substrate 1 (ESR1) without a predicted G4 motif (91) were PCR-amplified with HotStar Taq Polymerase (Qiagen). The PCR fragments were cleaned-up with Promega ReliaPrep DNA concentration kit and eluted in dH2O ...
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bioRxiv - Plant Biology 2023Quote: ... Sand and rhizosphere samples were prepared by adding 250 mg of the collected substrate to a PowerBead plate and homogenized using a TissueLyser II (Qiagen, USA) at a frequency of 20 Hz for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... RNA extraction was carried out at day 3 of culture on polyacrylamide substrates using an RNeasy Plus Micro Kit (Qiagen, 74034). This time point (different to the 6 day time point used in immunocytochemistry experiments ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA extraction for pools of five flies and the corresponding substrate was performed using the Qiagen QIAamp DNA extraction kit (Qiagen, Carlsbad, CA) combined with bead beating in the same way as for fly samples from Wang and Staubach (2018).