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Citations for Qiagen :
1 - 50 of 618 citations for TMEM173 Human HEK293 Sumo His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the His-SUMO conjugates were enriched using nickel-nitrilotriacetic acid beads (Qiagen). Upon multiple washing steps ...
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bioRxiv - Plant Biology 2022Quote: ... and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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bioRxiv - Cell Biology 2023Quote: ... Tmem173 siRNA was purchased from Qiagen (catalogue-number GS72512).
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bioRxiv - Cell Biology 2022Quote: ... His- tagged human UHRF1 was purified using Ni-NTA sepharose resin (Qiagen). Recombinant E1 (His-UBE1) ...
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bioRxiv - Cell Biology 2019Quote: ... E2 and SUMO were purified using Ni-NTA agarose resin (Qiagen) with 20 mM sodium phosphate pH7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... SUMO-tag and protease were removed using Ni-NTA beads (Qiagen) and the protein of interest dialyzed in a sucrose buffer (250 mM sucrose ...
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bioRxiv - Cell Biology 2023Quote: ... SUMO-PTEN were pulled down with Ni2+-NTA beads (#30210, Qiagen) and analyzed with SDS-PAGE as previous described (Huang et al. ...
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bioRxiv - Immunology 2021Quote: ... and HRP-anti-His (Penta-His Ab, Qiagen) was used in the detection step ...
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bioRxiv - Cell Biology 2020Quote: ... Bacterially expressed 6His-SUMO fusions were isolated on Ni-NTA resin (Qiagen) and eluted from the resin by direct cleavage of SUMO by Ulp1 ...
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bioRxiv - Molecular Biology 2019Quote: ... penta-HIS (QIAGEN) or α-RF1 E.coli (Zaher Lab ...
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bioRxiv - Cell Biology 2021Quote: ... penta-His (Qiagen). Membranes were then incubated with fluorophore-conjugated secondary antibodies diluted 1:20,000 in TBS-T and 5% BSA ...
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bioRxiv - Microbiology 2022Quote: ... α-His antibody (Qiagen) was used at 1:5,000 dilution to detect the presence of rRH5 in Native-PAGE.
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bioRxiv - Biophysics 2020Quote: ... anti-His antibodies from the Penta-His HRP Conjugate Kit (Qiagen) were used.
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bioRxiv - Biochemistry 2022Quote: ... anti-His antibodies from the Penta-His HRP Conjugate Kit (Qiagen) were used ...
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bioRxiv - Plant Biology 2022Quote: ... bound His-mSTIC2 protein was detected by anti His HRP conjugate (Qiagen) and ECL reaction (Pierce).
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bioRxiv - Cell Biology 2020Quote: ... Penta-His from QIAGEN; CD63 (clone H5C6 ...
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bioRxiv - Microbiology 2020Quote: ... His-Penta-Conjugate (Qiagen) was then used as the secondary antibody (1:5000) ...
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bioRxiv - Immunology 2020Quote: ... and detection was performed using anti-His antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse antibody labeled with AlexaFluor647 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... after transfection with MSCV-IRES-GFP constructs expressing His10-SUMO paralogs (Effecten reagent, Qiagen, #301425). GFP-positive cells with similar and weak fluorescent signals were sorted by flow cytometry ...
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bioRxiv - Biophysics 2023Quote: 6xHis-SUMO-Cys-GAF-DBD protein was first purified on Ni-NTA agarose (Qiagen 30210). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... His-EHD1 was purified by binding with Ni2+-NTA His-bind slurry (Qiagen, Germany) and eluting with imidazole as described previously (65) ...
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bioRxiv - Biophysics 2021Quote: ... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
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bioRxiv - Cell Biology 2021Quote: ... and detection was performed using anti-His primary antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse secondary antibody labeled with AlexaFluor647 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... and detection was performed using anti-His primary antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse secondary antibody labeled with AlexaFluor647 (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... and anti-His (Qiagen 34660) antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... anti-RGS-His (Qiagen, 34610), anti-Vinculin (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-penta-His (34660, Qiagen), anti-GFP-HRP (B-2 ...
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bioRxiv - Biochemistry 2021Quote: ... and anti-His (Qiagen 34660) antibodies ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells and MEFs were transiently transfected with PolyFect (Qiagen) and Lipofectamine 3000 (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged DmMIC10b was detected using an anti-His antibody (Qiagen N.V., Venlo, The Netherlands, 34660).
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bioRxiv - Cell Biology 2020Quote: ... His6-SUMO-WASHC2C-5-SNAP was purified using lysis buffer #2 and Ni-NTA-agarose beads (Qiagen). The protein was incubated with Ni-NTA beads and washed with 50 mM imidazole ...
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bioRxiv - Microbiology 2019Quote: ... and HRP-penta-His antibody (Qiagen) and HRP-anti-mouse IgG (Abcam ...
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bioRxiv - Cancer Biology 2021Quote: ... HIS-tag (Qiagen 34610 1:100) and HER3 (R&D Systems AF4518 1:400) ...
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bioRxiv - Microbiology 2019Quote: ... Penta His HRP conjugate (Qiagen, 34460) was diluted 1:5000 in 1% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse anti-His (Qiagen, number 34660); Mouse anti-β-actin (Proteintech ...
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bioRxiv - Biochemistry 2022Quote: ... A mouse monoclonal His-tetra (Qiagen) antibody (1:1000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-His Western blots were performed using Penta-His mouse monoclonal IgG1 as primary antibody (Qiagen, #34660) at 1:2000 dilution ...
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bioRxiv - Plant Biology 2023Quote: ... The His-ZmTPL2N-His recombinant protein was purified using Pierce Ni-NTA resin (QIAGEN, Cat. No. 30210) according to the product protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Transfection of HEK293 cells was performed using Attractene transfection reagent (Qiagen) by the fast-forward transfection approach following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... or EGFP-Lin52 fusions into HEK293-GP cells with Effectene (Qiagen) for transduction as described previously (58).
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bioRxiv - Biochemistry 2023Quote: ... Cleaved CK1δ ΔC was further purified away from His-GST and His-TEV using Ni-NTA resin (Qiagen) and subsequent size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-His tag (1:2000; QIAGEN) and rabbit anti-GST (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-His from Qiagen (1:10,000), home-made rabbit anti-VASH1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... primary mouse anti-His antibody (QIAGEN, 34660), secondary goat anti-rabbit 800CW antibody (LI-COR ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... the Penta-His HRP Conjugate Kit (Qiagen) was used following manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... the mouse monoclonal Penta-His antibody (Qiagen n°34660 stock at 200 µg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... anti-RGS-His (Qiagen, 1:2000, 34610), anti-Myc (ChromoTek ...
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bioRxiv - Biochemistry 2022Quote: ... Mouse anti-His (Qiagen, 34670; 1:200) and goat anti-mouse IgG-AF488 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... The Penta-His HRP Conjugate Kit (Qiagen) was used for detection of His-tagged proteins according to manufacturer’s instructions ...