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Citations for Qiagen :
1 - 50 of 59 citations for Sulfo NHS LC Biotin sodium since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 20 nM biotin-NTA (QIAGEN) was charged with 50 nM Ni2+ and incubated on the slide for 10 min before rinsing away the unbound excess biotin-NTA and Ni2+ with PBS (this step was omitted for the labeled DNA samples) ...
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bioRxiv - Neuroscience 2021Quote: ... NHS was removed with QIAquick Nucleotide removal kit (Qiagen, 28304) and the conjugated JF-669 hairpins were reconstituted with nuclease free water to a final concentration of 60 ng/ml (3 mM).
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bioRxiv - Cell Biology 2019Quote: ... conjugated with anti-penta-his biotin antibody (Qiagen) for 1 and half hours at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Penta-His-biotin conjugate 1:5000 (Qiagen) overnight ...
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bioRxiv - Microbiology 2021Quote: ... then sodium bisulfite-converted (EpiTect Bisulfite kit, Qiagen). The 5’LTR or the rev regions were amplified by (semi)nested PCR (primer sequences are available upon request) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and treated the pools with sodium bisulfite (EpiTect, Qiagen) following a protocol for formalin-fixed paraffin-embedded samples (Gu et al ...
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bioRxiv - Microbiology 2021Quote: ... was modified by a custom-added biotin residue at its 5’-end (Qiagen). Hybrids of the CoV-2 RNAs with the probe were detected with a goat anti-biotin antibody conjugated to 10 nm gold particles (BBI international).
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from 105 sorted LCs with the RNeasy Mini Kit (Qiagen). RNA integrity was evaluated on an Agilent Bioanalyzer 2100 (Agilent Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
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bioRxiv - Genetics 2023Quote: ... with biotin-labeled methylation-specific primers designed with Pyromark Assay Design Software 2.0 (Qiagen). The primer sequences are shown in Supplemental Table S1 ...
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bioRxiv - Plant Biology 2022Quote: ... and sodium bisulfite treated using the Epitect Bisulfite Conversion kit (QIAGEN). The region of interest within the MeSWEET10a promoter was amplified using the primers in Extended Data Table 3 ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... 0.005% sodium dodecyl sulfate) supplemented with 5LμL of Proteinase K (Qiagen). The cells were lysed by incubation at 55L°C for 1Lh ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... 0.005% sodium dodecyl sulfate) supplemented with 5LμL of Proteinase K (Qiagen) after the growth medium was removed ...
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bioRxiv - Molecular Biology 2021Quote: ... Unbound Biotin-HPDP was removed by chloroform/isoamylalcohol (24:1) extraction in MaXtract tubes (Qiagen). RNA was precipitated with 10th volume of 5M NaCl and 1 volume of isopropanol ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 50 mM EDTA and 50 mM EGTA to the NHS beads or Modified RLT buffer (1x Buffer RLT supplied by Qiagen, 10mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... or 10 ul buffy coat (from Accio Biobank or NHS) using the DNeasy Blood and Tissue Kit (Cat# 69504, Qiagen) following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... Unbound biotin was removed by performing a chloroform:isoamyl alcohol extraction using MaXtract High Density tubes (Qiagen). RNA was isopropanol precipitated and resuspended in water ...
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bioRxiv - Microbiology 2022Quote: ... Unbound biotin was removed by performing a chloroform:isoamyl alcohol extraction using MaXtract High Density tubes (Qiagen). RNA was isopropanol precipitated and resuspended in water ...
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bioRxiv - Biophysics 2023Quote: ... the glass was additionally treated with biotinylated anti-hexahistidine monoclonal antibody (Penta-His Biotin Conjugate; Qiagen) as in Duchi et al.23 and Dulin et al.31.
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bioRxiv - Microbiology 2023Quote: ... Unbound biotin was removed by performing a chloroform:isoamyl alcohol extraction using MaXtract High Density tubes (Qiagen). RNA was isopropanol precipitated and resuspended in water ...
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bioRxiv - Microbiology 2022Quote: ... Reference plasmids were serially diluted in either PBS buffer or heat-inactivated normal human serum (NHS) and re-extracted using the QIAamp® DNA Blood Mini Kit (Qiagen) per recommended protocol for serum ...
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bioRxiv - Cancer Biology 2019Quote: All tissues were dissociated in 100 mM sodium carbonate using TissueLyzer II (QIAGEN) for 0.5 min at a rate of 0.30 repetitions three times and stored on ice for 30 sec between the dissociation cycles ...
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bioRxiv - Physiology 2020Quote: ... with 0.1% (w/v) sodium dodecyl sulphate (SDS) with a Tissue Ruptor (Qiagen). Samples were centrifuged for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % (w/v) sodium deoxycholate) and once with 500 µl EB buffer (Qiagen). Beads were treated with 300 µg/ml RNase A ...
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bioRxiv - Microbiology 2020Quote: ... was purchased from NeoMarkers Inc. (Portsmouth, NH, USA). RNAprotect cell reagent (Cat. No. 76526) and RNeasy (Cat No./ID: 74106) were obtained from Qiagen (Hilden, Germany). MasterPure DNA purification kit (Cat ...
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bioRxiv - Cell Biology 2021Quote: ... Sodium bisulfite conversion of unmethylated cytosines in DNA was based on EpiTect Bisulfite Handbook (Qiagen). We designed primers (Table S3 ...
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bioRxiv - Biophysics 2021Quote: ... NF-κB molecules were immobilized through the 6xHis-tag on the C-terminus of RelA by penta-His antibody biotin conjugate (34440, Qiagen). For DNA-bound NF-κB experiments ...
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bioRxiv - Plant Biology 2021Quote: ... were amplified using biotin-labeled primers (Supplementary Table 2) synthesized by Sangon Biotech (Shanghai, China) and purified by a PCR purification kit (Qiagen). The EMSA was conducted as described previously(An et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... SiCBL4 and SiCBL7 promoters were respectively amplified using biotin-labeled primers (Table S1) synthesized by Sangon Biotech (Shanghai China) and purified using a PCR purification kit (Qiagen). EMSA was conducted using a LightShift Chemilumniescent EMSA Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Then this amplified product was labeled with biotin at 5’ end followed by purification using the Qiaquick nucleotide removal kit (QIAGEN).To check the drug-protein interaction ...
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bioRxiv - Cell Biology 2021Quote: ... Two μg of DNA was sodium bisulphite modified using the Eptech Kit (Qiagen GmbH, Hilden, Germany) in accordance with the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Cell pellets were resuspended in 50 mM sodium citrate buffer and treated with RNase A (QIAGEN) for 2 hours at 50°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 0.1 mM sodium orthovanadate and 0.2 mM phenylmethylsulfonyl fluoride (PMSF)) with a Tissue RuptorTM (Qiagen, Netherlands) homogenizer ...
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then incubated for ~ 10 min with 4 μg/ml of Penta•His biotin conjugate antibody (34440, Qiagen, UK) in reaction buffer [40 mM HEPES buffer (pH 7.3 ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μg of RNA was resuspended in 10 μl of RNAse free water and mixed with sodium bisulfite pH 5.0 (42.5 μl) and DNA protection buffer (17.5 μL) (EpiTect Bisulfite Kit, Qiagen). The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... Adapter-ligated DNA was treated with sodium bisulfite using the EpiTect Plus Bisulfite kit (QIAGEN, Hilden, Germany) and amplified using the Kapa HiFi Uracil + ReadyMix (Roche ...
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bioRxiv - Paleontology 2019Quote: ... DNA was then exposed to two rounds of sodium bisulfite treatment using the EpiTect Bisulfite kit (QIAGEN), and paired-end DNA sequencing was performed using the Illumina Hi-Seq 2000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The complex was initially crystallized in a buffer 0.1 M MES pH 6.5 and 0.6 M sodium fluoride from Anions Suite crystallization screen (Qiagen). After optimization of crystallization conditions ...
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bioRxiv - Molecular Biology 2019Quote: ... resuspended in 50 mM sodium citrate buffer containing 10 µl of RNase A (20 mg/ml, Qiagen 76254) and incubated for 2 hours at 50°C ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
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bioRxiv - Neuroscience 2021Quote: Five hundred ng of DNA was treated with sodium bisulfite using the EpiTect Fast DNA Bisulfite kit (Qiagen). Primers for nested PCR (2 fragments for HCRT ...
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bioRxiv - Microbiology 2021Quote: ... 1mM PMSF and 0.25% sodium deoxycholate and the clarified lysate was loaded on a Ni-NTA column (Qiagen). Non-specifically bound proteins were removed by washing with lysis buffer containing 20mM imidazole and the proteins eluted with buffer containing 150mM imidazole ...
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bioRxiv - Plant Biology 2019Quote: ... 500 ng of each native library were treated by sodium bisulfite using the EpiTect Bisulfite kit (Qiagen, Valencia, CA) and purified according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Genomics 2019Quote: ... De-crosslinked DNA was added with 10μl 3M sodium acetate pH 5.2 and purified with QIAquick PCR Purification kit (QIAGEN 28106) according to manufacture instruction ...
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bioRxiv - Plant Biology 2023Quote: ... Adaptor-ligated libraries were subjected to two successive treatments of sodium bisulfite conversion using the EpiTect Bisulfite kit (Qiagen) as outlined in the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Adaptor-ligated libraries were subjected to two successive treatments of sodium bisulfite conversion using the EpiTect Bisulfite kit (Qiagen) as outlined in the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: Total cell lysates were generated in RIPA buffer (1% Nonidet P40, 0.5% sodium deoxycholate, 0.1% SDS in PBS) by homogenization with a Tissuelyser TM (QIAGEN, Venlo, Netherlands) at a frequency of 30 Hz for 2 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Purified extracellular WT and Δtgif2k-b tachyzoites were treated with vehicle or 5 μM sodium arsenite for 2 h at 37°C and total RNA was extracted using RNeasy (Qiagen). The RNA concentration for each sample was measured using Nanodrop One (Thermo Scientific) ...