Labshake search
Citations for Qiagen :
1 - 50 of 113 citations for Steroid Receptor Co Factor Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Co-factors were purified using Ni-NTA agarose beads (Qiagen), eluting with 300 mM imidazole ...
-
bioRxiv - Bioengineering 2021Quote: ... supernatant was collected and Factor Xa was removed with Factor Xa Removal Resin (QIAGEN). Endotoxin was removed using endotoxin removal resin and Atsttrin purity was determined by SDS-PAGE and reverse phase high performance liquid chromatography (HPLC).
-
bioRxiv - Immunology 2020Quote: A RT2 profiler array for mouse inflammatory cytokines and receptors (Qiagen, USA) was used according to manufacturer recommendations to measure the expression of 84 inflammatory genes (Table S1) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... using a Qiagen DNAeasy kit (Qiagen Co). For each sample ...
-
bioRxiv - Genetics 2023Quote: ... RNeasy Plant Mini kits (QIAGEN Co., Hilden, Germany) were used for extraction ...
-
bioRxiv - Cancer Biology 2020Quote: ... Transcription factor prediction was performed with Ingenuity Pathway Analysis (Qiagen), iRegulon (Janky et al ...
-
bioRxiv - Immunology 2022Quote: ... For RT2 Profiler PCR Array Human Interferons and Receptors (Cat# PAHS064ZC-12, Qiagen, Germantown, MD), RNA extraction (RNeasy Pls Mini kit ...
-
bioRxiv - Neuroscience 2021Quote: ... and loaded into the Pig Inflammatory Cytokines & Receptors RT2 ProfilerTM PCR Array (Qiagen, Hilden, Germany). The qPCR reactions were performed using a Qiagen Rotor-Gene Q real-time cycler ...
-
bioRxiv - Biochemistry 2024Quote: ... protein purification was performed using Co+NTA agarose (Qiagen), followed by a Phenyl Sepharose column ...
-
bioRxiv - Immunology 2020Quote: ... cDNA was added to the Human Toll-like receptor signaling pathway RT2 Profiler PCR array (Qiagen) and run on an iCycler MyiQ (Bio-Rad) ...
-
bioRxiv - Cell Biology 2023Quote: Co-cultures were lysed in RLT Plus Lysis Buffer (Qiagen) and RNA isolation was performed with the RNeasy Mini Kit (Qiagen ...
-
bioRxiv - Microbiology 2019Quote: ... The RT2 Profiler “Inflammatory cytokines and receptors” and “Human innate and adaptive response” arrays PCR Arrays (Qiagen) were performed according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... co-precipitated RNAs were purified using the RNeasy Mini Kit (Qiagen). After the synthesis of first-strand cDNAs with 200 U of Superscript IV reverse transcriptase (Invitrogen ...
-
bioRxiv - Cancer Biology 2021Quote: ... Co-precipitated DNA was purified using a QIAquick spin column (QIAGEN), and 2 μL of DNA was analyzed via qPCR using specific primers for the ITGB1 promoter ...
-
bioRxiv - Cell Biology 2020Quote: ... pBI-UASc-APP695-EGFP was co-transfected using Effectene reagent (Qiagen) with pACSwitch (gift from M ...
-
bioRxiv - Developmental Biology 2022Quote: ... The IPA system (version 73620684, Ingenuity Systems; Qiagen China Co., Ltd.) was used for subsequent bioinformatics analysis ...
-
bioRxiv - Molecular Biology 2023Quote: ... 1.5 million cells were co-transfected with Effectene Transfection Reagent (Qiagen), according to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... Plasmids were transfected in COS-7 cells with Effectene (Qiagen, Germany) according to the manufacturer’s protocols for 22 hours ...
-
bioRxiv - Cancer Biology 2022Quote: A ready-to-transduce transcription factor-responsive lentiviral reporter system (CCS-1022L, QIAGEN) was used to generate a stable cell line ...
-
bioRxiv - Physiology 2020Quote: ... or the QuantiTect PCR Kit with self-designed primers for neuronal factors (Qiagen, Germany) (Supplementary Table 3) ...
-
bioRxiv - Microbiology 2021Quote: ... viral RNA was isolated with QIAzol Lysis Reagent (Qiagen Co. LTD, USA). After reverse-transcription by the SensiFAST™ cDNA Synthesis Kit (Bioline ...
-
bioRxiv - Biochemistry 2023Quote: ... plasmids were transiently co-transfected into HEK293T cells using PolyFect (Qiagen 301105) according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2020Quote: ... insulin-like growth factor 1 receptor (Igf1r) and sirtuin 1 (Sirt1) gene promoters were designed using the Pyromark Assay Deisgn 2.0 software (Qiagen). PCR and sequencing primers are provided in Supplementary Table 1 ...
-
Fatty acid binding proteins shape the cellular response to activation of the glucocorticoid receptorbioRxiv - Pharmacology and Toxicology 2021Quote: ... qPCR was performed using the RT2 SYBR Green Mastermix and Glucocorticoid Receptor Signalling RT2 Profiler PCR Array from Qiagen, according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Co-immunoprecipitated DNA fragments were purified using the QIAquick PCR purification kit (Qiagen) and analysed by quantitative PCR targeting the promoter elements of NFKBIA ...
-
bioRxiv - Plant Biology 2019Quote: ... The supernatant was incubated on a rotator with Ni-NTA Agarose (Qiagen Co.) for 1.5 hours at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from co-cultured cells using the RNeasy Kit (QIAGEN). cDNA was generated by using the Transcriptor First Strand cDNA Synthesis Kit (Roche) ...
-
bioRxiv - Immunology 2021Quote: ... and an undisclosed peptide pool inducing CD8+ T lymphocyte stimulation (Qiagen, 2017); rmsHBHA which tubes contain recombinant M ...
-
bioRxiv - Molecular Biology 2020Quote: ... total RNA was isolated from 1×106 BMMCs that were untreated or treated with IgE receptor crosslinking using the RNeasy mini kit (Qiagen). RNA was treated with DNase I according to manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... All plasmids were verified by sequencing of the full receptor cDNA and purified with an EndoFree Plasmid Maxi Kit (Qiagen). cDNA sequences of all GPCR variants cloned in pcDNA3.1 are summarized in Data S2.
-
bioRxiv - Cell Biology 2023Quote: ... which is based on the expression of known ligand-receptor pairs112.Gene lists were imported into the IPA software Ingenuity Pathway Analysis (IPA) 01.12 (Qiagen Bioinformatics) to assess pathway/biological function enrichment analysis.
-
bioRxiv - Genetics 2024Quote: ... and a negative control region within a regulatory region of the estrogen receptor substrate 1 (ESR1) without a predicted G4 motif (91) were PCR-amplified with HotStar Taq Polymerase (Qiagen). The PCR fragments were cleaned-up with Promega ReliaPrep DNA concentration kit and eluted in dH2O ...
-
bioRxiv - Cell Biology 2024Quote: ... a second transfection of a 0.6 μg plasmid encoding either the C-terminal GFP11 tagged M2tail(368-466) or M2 receptor was performed using the Effectene Transfection Reagent (Qiagen). After washing each well with 2 mL PBS ...
-
bioRxiv - Molecular Biology 2020Quote: The consensus sequences of piRNA biogenesis factors were assembled using CLC Genomics Workbench version 8.0.1 (QIAGEN) and confirmed by published homolog sequences using BLASTp ...
-
bioRxiv - Cancer Biology 2022Quote: ... The transcription factor prediction was performed using i-cisTarget20 and the upstream regulator function by QIAGEN Ingenuity Pathway Analysis.21 Differentially expressed genes (DEGs ...
-
bioRxiv - Immunology 2023Quote: ... single or co-cultures of monocytes and platelets were lysed in RLT Buffer (Qiagen) containing β-Meracptoethanol (1 x 104 cells/µl) ...
-
bioRxiv - Plant Biology 2024Quote: ... samples were cleaned-up using the QIAquick PCR Purification Kit (Qiagen GmbH & Co. KG). Samples were eluted from the column in a 30 μl volume and analysed by RT-qPCR with specific primers (Supplemental Table S3).
-
bioRxiv - Immunology 2021Quote: ... Gene expression profiling of inflammatory cytokines and receptors of normal and KP-OVA tumor bearing lungs was performed by custom RT2 Profiler PCR Array (Qiagen, cat. 330221) following manufacturer’s instructions.
-
bioRxiv - Immunology 2022Quote: ... and the cDNA was loaded onto the “Mouse Toll-Like Receptor Signaling Pathway” RT2 Profiler PCR Array according to the manufacturer instructions (Qiagen, Valencia, CA). Expression of the target genes were normalized to the geometric mean of Ct values of the two housekeeping genes Gusb and Hsp90a using the ddCt method (n = 3) ...
-
bioRxiv - Plant Biology 2019Quote: ... and purified from an overnight culture using DNA Mini prep kit (Qiagen Co., Valencia, CA).
-
bioRxiv - Cell Biology 2019Quote: ... Co-NTA beads were obtained from stripping 1 mL of Ni-NTA resin from Qiagen in a Bio-rad gravity column with 50 mL of 0.5M EDTA (pH 8.0) ...
-
bioRxiv - Developmental Biology 2020Quote: ... The pAct-Gal4 and pUAST-bab2CD plasmids were co-transfected using Effectene Transfection Reagent (QIAGEN) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2023Quote: ... succinogenes strain UWB7 monoculture & co-culture pellets using the RNeasy Mini Kit (Qiagen, Germantown, MD) following the protocol for purification of total RNA from plant cells and tissues and filamentous fungi ...
-
bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from co-cultures after 20 hours using the RNeasy kit (Qiagen). One μg of total RNA was reverse transcribed using iScript cDNA synthesis kit (Bio-Rad) ...
-
bioRxiv - Immunology 2023Quote: ... RNA from the co-culture experiment was extracted using the RNeasy UCP Micro Kit (Qiagen), and reverse transcribed using a high-capacity cDNA reverse transcription kit (Applied Biosystems) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The peptide-coupled proteins were separated from uncoupled proteins using Ni-NTA Agarose (Qiagen).
-
bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
-
bioRxiv - Molecular Biology 2019Quote: ... cells were co-transfected with the C6orf203-TAP construct and with plasmid pOG44 using Superfect (Qiagen). After 48h ...
-
bioRxiv - Cell Biology 2022Quote: COS-7 cells were transfected with the respective Myc-Sun4-pEGFP fusion constructs using EffecteneTM (Qiagen) following the manufacturer’s protocol and incubated overnight before analysis of the ectopically expressed fusion proteins.
-
bioRxiv - Cell Biology 2021Quote: Cells were co-transfected with the different plasmids using Effectene reagent according to manufacture indications (Qiagen). For stable transfection ...