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Citations for Qiagen :
1 - 50 of 702 citations for Retinol Binding Protein RBP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Recombinant protein was purified by binding to Ni-NTA beads (Qiagen) and eluted in the same buffer supplemented with 300 mM imidazole ...
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bioRxiv - Physiology 2019Quote: ... in 40 μl Binding Buffer (Pyromark binding buffer, Qiagen, Manchester UK) and 25 μl Milli-Q-water ...
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bioRxiv - Molecular Biology 2024Quote: ... PyroMark binding buffer (Qiagen), and 1 μL Streptavidin sepharose TM high performance beads (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: A Notch pathway transcriptional output reporter cell line was generated by infecting MCF10A cells with Cignal Lenti RBP-Jk Reporter (luc) lentiviral particles (Qiagen, Cat #: CLS-014L) following manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampi were placed in protein low-binding tubes mounted onto a Qiagen TissueLyser II (Qiagen cat. Nr. 85300). Lysates were span down at 20.000 rcf for 20 minutes at 4°C to remove debris ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNA expression levels were normalized to the housekeeping human TATA-binding protein (TBP) mRNA (RT2 qPCR Primer Assays, Qiagen) in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT ...
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bioRxiv - Microbiology 2021Quote: ... and H2A.Z (fused with a maltose-binding protein -MBP) purification were performed by affinity chromatography using Ni-NTA agarose (Qiagen) and amylose resin (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: The assessment of ligand binding was performed via shifts in protein thermostability upon binding using thermal denaturation on a rotary quantitative PCR (qPCR) instrument (Qiagen Rotor-Gene Q 2plex HRM ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... After binding to Ni-NTA Superflow (Qiagen) resin ...
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bioRxiv - Evolutionary Biology 2020Quote: ... purification step with 10x binding buffer PB (Qiagen) was carried out between the first and second steps.
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bioRxiv - Genetics 2020Quote: ... 440 μl of DNA binding buffer beads (Qiagen) were added to the sample and mixed by pipetting ...
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bioRxiv - Immunology 2022Quote: ... coli lysate by binding to Ni-NTA (QIAGEN) and gravity chromatography ...
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bioRxiv - Neuroscience 2023Quote: ... 440 μl of DNA binding buffer beads (Qiagen) were added to the sample and mixed by pipetting ...
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bioRxiv - Genetics 2022Quote: ... PCR products were purified using PyroMark binding buffer (Qiagen) and 1 μl Streptavidin Sepharose TM high-performance beads (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... and binding of sample to an RNeasy mini kit column (Qiagen). RNA was washed and eluted according to the manufacturer’s instructions and the concentration was measured using a NanoDrop 2000 (Thermo Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... 1 ml binding buffer (provided in Qiagen PCR purification kit, #28106) and 20 µl of 3 M sodium acetate pH 5.5 (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA binding and purification was performed using RNeasy Mini Kit (Qiagen). Total RNA was extracted with RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... His6-MipZ was purified by binding to Ni-NTA agarose (Qiagen), washed in Ni wash buffer (Ni lysis buffer with 20 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... Pre-equilibrated nickel-NTA resin (Qiagen, 50 mg/mL binding capacity) was added to the clarified lysate and the lysate-resin slurry stirred in a beaker with a stir bar for 1 hour at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... After 14 h binding to 4 mL Ni-NTA agarose beads (Qiagen), the beads were first washed with 15 mL Ni-NTA binding buffer and then with 15 mL Ni-NTA wash buffer (1 M NaCl ...
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bioRxiv - Immunology 2021Quote: ... 30 μl Ni2+ beads equilibrated in binding buffer (Qiagen Ni-NTA superflow) was added ...
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bioRxiv - Biophysics 2021Quote: ... Supernatant was collected for 2.5 h binding with Ni-nitrilotriacetic acid resins (Qiagen) at 4°C ...
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bioRxiv - Genomics 2019Quote: ... the supernatant extracted and subjected to batch binding with 5ml of NiNTA (Qiagen) for 2hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... according to the manufacturers’ instructions and purified through a DNA-binding column (Qiagen). The quality and quantity of cDNA was measured using a Nanodrop spectrophotometer (Nanodrop Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting supernatant was subject to binding with Ni-NTA agarose resin (Qiagen) for 30 min at 4 °C with gentle rotation and loaded into the glass chromatography columns (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... His-EHD1 was purified by binding with Ni2+-NTA His-bind slurry (Qiagen, Germany) and eluting with imidazole as described previously (65) ...
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bioRxiv - Bioengineering 2022Quote: ... 0.01M Tris-Cl (pH 8.0) and passed through a Ni-NTA binding column (Qiagen). The loaded column was washed with 8M urea ...
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bioRxiv - Biochemistry 2022Quote: ... The detection of binding was carried out by the RGS His6 HRP conjugate (QIAGEN) that detects the RGS His6 motif present in the affitins N-terminal ...
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bioRxiv - Cell Biology 2022Quote: ... The mRNA binding to beads was eluted and purified with RNeasy MinElute Spin columns (Qiagen), followed by cDNA synthesis ...
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bioRxiv - Cell Biology 2022Quote: ... Binding of Ad2 was detected by an HRP-conjugated monoclonal antibody to His-tag (Qiagen) expressed by the fiber knob ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using a 10:1 ratio of binding buffer (a modified version of Qiagen’s PB buffer) to digestive mixture ...
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bioRxiv - Genomics 2023Quote: ... Reactions were stopped by adding the appropriate volume of Binding Buffer (Qiagen MinElute PCR Kit) and the DNA was purified using the Qiagen MinElute PCR Kit according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Gravity column was used for purification with His6-tagged Mpro binding to Ni-NTA beads (Qiagen 30210), washed with lysis buffer + 10mM imidazole and eluted with increasing concentration of imidazole (50mM ...
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bioRxiv - Microbiology 2021Quote: ... Frozen lung sections were homogenized in 1 ml of Lysis/Binding solution in a TissueLyser II (Qiagen) with three cycles of at 30 Hz for 30 seconds ...
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bioRxiv - Developmental Biology 2020Quote: ... and the Tbx6VE+ and Tbx6VE- fraction was sorted in 1.5ml low-binding tubes with 350μl RLT (Qiagen) + 1% v/v B-mercaptoethanol (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... the list of binding partners was annotated using Ingenuity Pathway Analysis (IPA, www.qiagen.com/ingenuity; Qiagen; March 2019) tool.
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bioRxiv - Molecular Biology 2023Quote: ... bead-bound DNA was washed twice with cold binding buffer and three times with EB buffer (Qiagen).
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bioRxiv - Genomics 2019Quote: ... The mixture was then passed through the RNA binding column of RNeasy Mini Kit (74104, Qiagen, Germantown, MD), followed by washing and elution steps described in the manufacture’s handbook.
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bioRxiv - Cancer Biology 2021Quote: ... Individual tumors were collected in low binding DNA tubes and digested in 3 μl RLT buffer (Qiagen Cat# 1048449) for 30min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... clarified samples were transferred to a 50 mL conical tube and supplemented with a final 20 mM imidazole pH 7.5 before batch-binding with Ni-NTA superflow resin (Qiagen) for ∼1 hr at 4°C ...
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bioRxiv - Genetics 2021Quote: ... followed by protein precipitation with the Protein Precipitation Solution (Qiagen) for 10 min at -20 °C ...
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bioRxiv - Neuroscience 2019Quote: ... 200 μl of ethanol was added to the samples prior to binding to Qlamp spin column (QIAamp DNA Blood mini kit; Qiagen) and samples were centrifuged at 8000 rpm for 1 minute ...
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bioRxiv - Systems Biology 2020Quote: ... and RNA seqFISH primary probe pools (1-10 nM per probe) and 10 nM polyT LNA oligo with a readout probe binding DNA sequence (Qiagen) were hybridized in 50% hybridization buffer consisted of 50% formamide (Invitrogen AM9342) ...
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bioRxiv - Microbiology 2020Quote: ... VSG2-antibody negative clones (positive transfectants were identified by lack of binding) were sequenced: RNA was isolated using the RNeasy Mini Kit including an on-column DNase treatment (Qiagen). Complementary DNA was synthesized with SuperScript IV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: The heatmaps relative to the ERK-dependent AP-1 target genes were generated by interrogating the Transcription Factor Target (TFT) collection (MSigDB) and TF binding site database by QIAGEN with the top DEGs between control and PD-treated Acomys ...
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bioRxiv - Cancer Biology 2021Quote: ... homogenized as described in the ‘RNA sequencing’ section were passed through the RNA binding column from the AllPrep DNA/RNA Mini kit (Qiagen) and the flow through was collected for protein precipitation ...
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bioRxiv - Biochemistry 2022Quote: ... The peptide spot membrane was blotted onto a nitrocellulose membrane and UNC-45 binding was probed with an anti-Strep antibody (Qiagen) using an ECL-kit for detection (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... pH was adjusted to 8 after resuspension to allow the binding of 6x His-tagged toxins to Ni-NTA agarose resin (reference: 30210; Qiagen). Resulting samples were passed through chromatography columns containing the Ni-NTA agarose resin and were eluted with denaturing buffer containing increasing concentrations of Imidazole (10 mM ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The remaining supernatant was mixed with 13x its volume of binding buffer and DNA was purified with MinElute columns (Qiagen), following the manufacturer’s instructions with the exception of a 15-minute incubation at 37□°C during the elution step ...