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Citations for Qiagen :
1 - 50 of 1963 citations for Recombinant Human Lectin Galactoside Binding Soluble 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The soluble recombinant protein was purified using theNickel-Nitrilotriacetic acid (Ni-NTA+; Qiagen) resin ...
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bioRxiv - Immunology 2023Quote: ... Recombinant protein was purified by binding to Ni-NTA beads (Qiagen) and eluted in the same buffer supplemented with 300 mM imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was cleared via centrifugation and recombinant PfCen3-6xHis were purified from the soluble fraction using Ni-NTA agarose beads (QiaGen) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... The soluble extracts were applied to a 1 ml of Ni2+- NTA-agarose (Qiagen) column that had been equilibrated with buffer A containing 10 mM imidazole ...
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bioRxiv - Physiology 2019Quote: ... in 40 μl Binding Buffer (Pyromark binding buffer, Qiagen, Manchester UK) and 25 μl Milli-Q-water ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ml binding buffer (provided in Qiagen PCR purification kit, #28106) and 20 µl of 3 M sodium acetate pH 5.5 (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNA expression levels were normalized to the housekeeping human TATA-binding protein (TBP) mRNA (RT2 qPCR Primer Assays, Qiagen) in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT ...
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bioRxiv - Cell Biology 2021Quote: ... Soluble lysate was incubated with NiNTA resin (Qiagen) equilibrated in NiNTA Wash Buffer A (50 mM HEPES•NaOH ...
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bioRxiv - Molecular Biology 2024Quote: ... PyroMark binding buffer (Qiagen), and 1 μL Streptavidin sepharose TM high performance beads (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... Soluble protein was mixed with Ni-NTA resin (Qiagen) for 1h at 4 degrees on a nutator ...
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bioRxiv - Molecular Biology 2024Quote: ... soluble fraction and Ni-NTA matrix agarose (Qiagen, Germany) were kept for binding for two hours at 4ºC ...
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bioRxiv - Microbiology 2020Quote: ... The soluble fraction was incubated for 1 hour at 4 °C using a Ni-NTA resin (Qiagen). The Ni-NTA resin was washed with 4 times resin volume (RV ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was applied to a nickel column (Qiagen) pre-equilibrated with RB ...
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bioRxiv - Biochemistry 2021Quote: ... Soluble cell lysate was purified using a Ni2+-nitrilotriacetate (QIAGEN) column ...
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bioRxiv - Biophysics 2023Quote: ... The soluble fraction was incubated with Ni-NTA resin (Qiagen) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... and the soluble fraction transferred to Ni-NTA beads (QIAGEN) in a gravity flow column ...
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bioRxiv - Biochemistry 2023Quote: ... Soluble 6His-Asf1 was removed on a NiNTA column (Qiagen) equilibrated in the LB5 buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Soluble fraction was incubated with Ni-nitrilotriacetic acid (NTA)-agarose (Qiagen) beads or glutathione-sepharose (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fractions were incubated with Ni-NTA Agarose resin (QIAGEN), previously equilibrated with equilibrium buffer (200 mM NaCl ...
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bioRxiv - Biophysics 2021Quote: ... The soluble fraction was purified by Immobilized Metal Affinity Chromatography (Qiagen) followed by FPLC size-exclusion chromatography (Superdex 75 10/300 GL ...
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bioRxiv - Synthetic Biology 2020Quote: ... The soluble fraction was purified by Immobilized Metal Affinity Chromatography (Qiagen) followed by FPLC size-exclusion chromatography (Superdex 75 10/300 GL ...
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bioRxiv - Developmental Biology 2022Quote: ... The soluble fraction was purified by immobilized metal affinity chromatography (Qiagen) followed by FPLC SEC (Superdex 75 10/300 GL ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was incubated with a Streptactin sepharose (Qiagen 1057979) for 3 hours ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using a 10:1 ratio of binding buffer (a modified version of Qiagen’s PB buffer) to digestive mixture ...
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bioRxiv - Biochemistry 2020Quote: ... After binding to Ni-NTA Superflow (Qiagen) resin ...
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bioRxiv - Molecular Biology 2021Quote: ... The soluble fraction was resuspended with preequilibrated Ni-NTA resin (Qiagen, 1018600) and incubated for 1 hr at 4 °C with end-to-end rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... The soluble fraction was resuspended with preequilibrated Ni-NTA resin (Qiagen, 1018600) and incubated for 1 hr at 4 °C with end to end rotation ...
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bioRxiv - Biochemistry 2021Quote: ... The soluble fraction was transferred to NiNTA agarose (Qiagen GmbH, Düsseldorf, Germany) equilibrated in 50 mM Na2HPO4/KH2PO4 ...
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bioRxiv - Biochemistry 2024Quote: ... After the separation of nanodiscs and soluble proteins on Ni-NTA (Qiagen), the same proportions of unbound material and imidazole-eluted material were analyzed (Fig.S5) ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant GST-Dnmt5(1-150)-6xHis was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 66,030 cm-1 M-1) ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant MBP-Dnmt5(1-150)-6xHis was purified with Ni-NTA agarose resin (Qiagen), measured by A280 (ε = 89,590 cm-1 M-1) ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...
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bioRxiv - Evolutionary Biology 2020Quote: ... purification step with 10x binding buffer PB (Qiagen) was carried out between the first and second steps.
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bioRxiv - Genetics 2020Quote: ... 440 μl of DNA binding buffer beads (Qiagen) were added to the sample and mixed by pipetting ...
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bioRxiv - Immunology 2022Quote: ... coli lysate by binding to Ni-NTA (QIAGEN) and gravity chromatography ...
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bioRxiv - Neuroscience 2023Quote: ... 440 μl of DNA binding buffer beads (Qiagen) were added to the sample and mixed by pipetting ...
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bioRxiv - Molecular Biology 2020Quote: ... Afterward soluble fraction RNA was then extracted using RNeasy Mini Kit (Qiagen, 74104) and cDNA synthesis with Superscript IV RT system (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... and the soluble protein fractions were purified by Ni-NTA resin (Qiagen, # 30230). The purified fraction was concentrated ...
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bioRxiv - Biochemistry 2020Quote: The soluble fraction was first purified by immobilized metal affinity chromatography (Qiagen # 30230) using gravity flow at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Soluble ACE 2 was purified using Nickel -NTA agarose beads (Qiagen Cat.No-1018244), eluted with 200mM imidazole ...
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bioRxiv - Synthetic Biology 2020Quote: ... The soluble fraction was purified by affinity chromatography over Ni-NTA Agarose (Qiagen) gravity columns ...
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bioRxiv - Biochemistry 2021Quote: ... and the soluble fractions were purified using nickel affinity chromatography (Qiagen, Manchester, UK) and eluted with lysis buffer containing 300 mM imidazole ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... the soluble extracts were subject to Ni-NTA agarose resin (QIAGEN cat#30210). After passing 20 volumes of washing buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... His-Hcp was purified from the soluble fraction by NTA-resin chromatography (Qiagen). Purified protein was desalted with a PD-10 desalting column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Frozen lung sections were homogenized in 1 ml of Lysis/Binding solution in a TissueLyser II (Qiagen) with three cycles of at 30 Hz for 30 seconds ...
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bioRxiv - Developmental Biology 2020Quote: ... and the soluble homogenates were purified by Ni-nitrilotriacetic acid (NI-NTA) Agarose (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The soluble hEKL proteins were purified using a Ni-NTA agarose resin (Qiagen, Germany) and subsequently HiLoad 16/60 superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... The soluble protein fraction was then purified with pre-equilibrated Ni-NTA resin (Qiagen), recovered in elution buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Soluble material was then were added to NTA nickel resin (400 µL slurry, Qiagen), which was pre-charged (overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... and the soluble fraction was incubated with Ni-NTA agarose beads (0.5 ml; Qiagen) for 3 hrs ...