Labshake search
Citations for Qiagen :
1 - 50 of 10000+ citations for Rat Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... myr-sfGFP translational reporters (described below) or myr-Venus using Effectene (Qiagen), as previously described2 ...
-
bioRxiv - Microbiology 2022Quote: ... Cytochrome b products were excised and purified from the gel using a commercial kit (QIAquick Gel Extraction Kit, Qiagen, Germany), followed by purification of eluted DNA using AMPure XP Magnetic Beads (1X ...
-
bioRxiv - Immunology 2023Quote: We incubated MAECs with glucose oxidase and collected the RNA 24h after the stimulus using the RNeasy Mini Kit from QIAGEN. Four samples per experimental group (control ...
-
bioRxiv - Cancer Biology 2019Quote: ... for 1 h at 37 °C and purified using QIAquick PCR Purification Kit (Qiagen). The pLVX-Puro lentiviral expression vector (Clontech ...
-
bioRxiv - Immunology 2020Quote: ... ConM-SOSIP.v7 carrying a C-terminal His-tag (diluted to 6.5nM in TBS) was immobilized onto 96-well Ni-NTA ELISA plates (Qiagen) by incubation for 2 hours at room temperature after which the plates were washed 3 times with TBS ...
-
bioRxiv - Neuroscience 2020Quote: ... Predicted upstream activators of the transcriptome were determined using Ingenuity Pathway Analysis software (Qiagen).
-
bioRxiv - Genetics 2021Quote: Multi-array ELISA was performed using the Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Samples were then centrifuged and plasma isolated and stored at -20°C prior to testing in the QuantiFERON IFNγ ELISA (Qiagen). Acceptance criteria for the assay specified by manufacturers were always met.
-
bioRxiv - Immunology 2022Quote: ... at 50 °C for 1 hour and were purified with a MinElute PCR Purification Kit (QIAGEN).
-
bioRxiv - Developmental Biology 2024Quote: ... using a touchdown PCR for the first 10 cycles from 72 to 60 followed by 35-40 cycles at the proper annealing temperature (Tm −2°C) and extension 68°C 30sec/Kb or 72°C 15sec/Kb and purified using a PCR purification KIT (Qiagen). Equimolar amounts of PCR products were mixed and a PCR was made with a primeSTAR GXL DNA polymerase (Takara Bio ...
-
bioRxiv - Neuroscience 2020Quote: ... Total RNAs were isolated from cerebral cortices (P15 rats) using Mini RNeasy kit (Qiagen) then converted to cDNA using 1 µg RNA and a QuantiTect Reverse Transcription kit (Qiagen ...
-
bioRxiv - Microbiology 2019Quote: ... at 37°C for 1 hour and cleaned up with the QIAquick PCR Purification Kit (Qiagen, Valencia, CA) according to manufacturer instructions ...
-
bioRxiv - Biophysics 2020Quote: ... or DNAse (200 Units well−1, 1 hr, 37°C, Qiagen). DAPI was stained at 0.5 μM (5 mins ...
-
bioRxiv - Molecular Biology 2020Quote: ... DNA was isolated from rat blood cells using QIAmp DNA Mini Kit (Qiagen, City, state) following manufacturer’s instructions using a QIAcube automated device ...
-
bioRxiv - Microbiology 2022Quote: ... and stored pellets at −80 °C for <1 week before RNA extraction using RNeasy Protect Bacteria Mini Kit (Qiagen) according to the manufacturer’s instructions (protocol #5) ...
-
bioRxiv - Genetics 2021Quote: ... and end-point PCRs were performed following 34 cycles (94 C 30 sec, 58 C 30 sec, 72 C 1 min) using the HotStarTaq Plus DNA polymerase (Qiagen, Canada) according to manufacturer’s protocol.
-
bioRxiv - Microbiology 2022Quote: ... were inoculated in 20 mL tryptone soy broth and incubated at 37°C for 24 h before 1 mL was pelleted and DNA extracted using the DNeasy Blood & Tissue kit (Qiagen). Libraries for genome sequencing were prepared using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs ...
-
bioRxiv - Physiology 2021Quote: ... Shredded bone marrow cells were frozen (−80°C) in RLT buffer (1% beta-mercaptoethanol) until RNA extraction using RNAeasy mini kit (Qiagen). RNA integrity number (RIN ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR fragment was then digested with Dpn I at 37 °C for 1 h and purified using the QIAquick PCR purification kit (Qiagen). To prepare the competent SW102 containing the pSMART BAC-T7-scv2-replicon ...
-
bioRxiv - Genetics 2021Quote: ... In vitro transcribed mRNAs were DNAse treated using 1 μl TURBO-DNAse for 20 minutes at 37 °C and purified using RNeasy Mini Kit (Qiagen). mRNA product was quantified using NanoDrop (Thermo Fisher ...
-
bioRxiv - Neuroscience 2021Quote: ... and 0.4 mg/mL Proteinase K (55°C for 1 hour) before being purified using the QIAquick PCR purification kit (QIAGEN). qPCR was performed on the Rotor-Gene 3000 (Corbett Life Science ...
-
bioRxiv - Microbiology 2022Quote: ... from 1 mL of culture were frozen at −80°C for later DNA extraction using the DNeasy PowerSoil Kit (Qiagen) according to the manufacturer’s procedure ...
-
Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6int and CCR6high subsets of CD4+ T cells were used for isolating RNA without and with prior activation with 20 ng/ml PMA and 1 mM ionomycin for 3 h at 37°C under 5% CO2 using RNeasy Mini Kit (Qiagen) and gene expression levels were determined using Illumina Human WG6 Expression BeadChip Version 3 arrays (Illumina Inc. ...
-
bioRxiv - Immunology 2023Quote: ... Cells were stimulated with LPS (1 μg/ml) for 6 hours at 37°C followed by RNA isolation using a RNeasy Kit (QIAGEN). Extracted RNA was converted to cDNA with an iScript cDNA Synthesis Kit (Bio-Rad) ...
-
bioRxiv - Neuroscience 2020Quote: Total hippocampus RNA was isolated from 4-month olds rats using RNeasy Lipid Tissue Kit (Qiagen) as per manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... DNA was extracted from plant cells and Hi-C libraries were prepared using EpiTect Hi-C Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2020Quote: ... coli cells grown overnight at 30°C (Plasmid Mini Kit, QIAGEN), and its concentration measured by nanodrop ...
-
bioRxiv - Microbiology 2022Quote: ... at −80 °C using the RNeasy plus Mini Kit (Qiagen, 74134) according to the manufacturers protocol ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... coli C mutants was extracted using DNeasy Blood & Tissue kit (Qiagen). Quality of extracted DNA was confirmed before sequencing ...
-
bioRxiv - Zoology 2020Quote: ... SDS and 100 µg/ml proteinase K) and incubated at 56°C for 1 h before extraction using a QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s directions ...
-
bioRxiv - Cell Biology 2022Quote: ... were digested with DpnI for 1 hour at 37°C and subsequently purified with the QIAquick Gel Extraction Kit (Qiagen #28704). They were ligated with homemade Gibson Master Mix (Table S1 ...
-
bioRxiv - Zoology 2023Quote: ... The mixtures were incubated at 56 °C for 1 h before DNA was extracted using an QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: We isolated genomic DNA from rat tail tips using Qiagen genomic DNA extraction kit (Qiagen Inc., USA). We performed genotyping via PCR ...
-
bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from rat or mouse hippocampal cultures using the RNeasy Mini Kit (Qiagen, #74104). DNA contamination was removed by digestion with DNase I (Qiagen ...
-
bioRxiv - Immunology 2021Quote: Total DNA was extracted from Wistar rat tissues samples using the DNeasy Blood & Tissue Kit (Qiagen, Germany) as per manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: RNA was isolated from rat lower esophageal tissues using the RNeasy Fibrous Tissue Kit (Qiagen, Germantown, MD). Each sample was homogenized in 400 µL of Buffer RLT with beta-mercaptoethanol for 3 – 10 second pulses with the homogenizer (Pro-Scientific Inc. ...
-
bioRxiv - Developmental Biology 2023Quote: ... genomic DNA was extracted from rat PSC cell pellets using the DNeasy Blood and Tissue kit (Qiagen) and PCR was performed using the Platinum PCR SuperMix High-Fidelity kit (Invitrogen) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... pH 5.8) under long day condtions (16h light) at 24 °C (day) 22 °C (night) using the DNeasy Plant Kit (Qiagen). ONSEN copy numbers were determined by qPCR using 12 ng total DNA using the KAPA SYBR FAST master mix universal on a C1000 Touch (Bio-Rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... Genomic DNAs were purified from 3 mL overnight cultures grew at optimum temperature (30 °C or 37 °C) by Dneasy Blood & Tissue Kits (QIAGEN). Additionally ...
-
bioRxiv - Microbiology 2023Quote: ... Eluates were then treated with Proteinase K (45°C, 1400rpm, 4h) and RNaseA (37°C, 1400rpm, 30min) before cleanup using MiniElute PCR Purification kit (Qiagen). Purified DNA together with input samples before immunoprecipitation were amplified using qPCR SyBr green mix (PCR Biosystems ...
-
bioRxiv - Microbiology 2021Quote: ... After 24 hours the media was removed for ELISA and the cells were lysed and RNA extracted (RNeasy Mini Kit, Qiagen).
-
bioRxiv - Biophysics 2019Quote: ... overnight at 37 °C and purified by a PCR purification kit (Qiagen). Subsequently ...
-
bioRxiv - Neuroscience 2020Quote: ... at −80°C until RNA extraction using the RNeasy Mini Kit (Qiagen). RNA quality was determined using the RNA nano assay on a 2100 Bioanalyzer (Agilent Technologies) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... pH 7.5 at 4°C) and purified using the RNAeasy kit (Qiagen).
-
bioRxiv - Developmental Biology 2022Quote: ... was linearized with BbsI-HF restriction enzyme for 1 hour at 37°C and gel purified with QIAquick® Gel Extraction Kit (Qiagen, 28704). Two gRNAs targeting ITGBβ1 (Horlbeck et al. ...
-
bioRxiv - Microbiology 2021Quote: ... Triton X-100 1.2% and lysozyme 20mg/ml) and incubated at 37°C for 1 h and DNA was extracted with the DNeasy blood&Tissue kit (Qiagen, Courtaboeuf, France) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... at 37 °C for 1 h and recovered by using RNeasy MinElute Cleanup kit as recommended by the manufacturer (Qiagen, Hilden, Germany). RNA yields were estimated by employing a Qubit® Fluorometer as recommended by the manufacturer (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2022Quote: ... 4°C) and stored at −80°C before metagenomic extraction using the DNeasy PowerSoil kit following manufacturer specifications (QIAGEN, Hilden, Germany). All sampling and sample processing was conducted following cold chain principles ...
-
bioRxiv - Immunology 2020Quote: ... A total of 78 serum samples (positive for HEV IgM in ELISA) were subjected to RNA extraction using QIAamp Viral RNA Mini Kit (Qiagen, Germany). Using SuperScript™ III One-Step RT-PCR System with Platinum™ Taq (Invitrogen ...
-
bioRxiv - Molecular Biology 2021Quote: ... 40 min, 4°C) prior to incubation (1 h, 4°C) with 4.0 mL of Ni-NTA agarose (Qiagen) pre-equilibrated in the same buffer ...