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Citations for Qiagen :
1 - 50 of 5462 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... according to the transfection-protocol of primary smooth muscle cells with siRNA using HiPerFect Transfection Reagent according to the manufacturer’s instructions (Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... primary muscle progenitor cells were transfected with 100nM of miR-24-3p mimic (Syn-mmu-miR-24-3p, Qiagen, MSY0000219), 100nM of miR-24-3p inhibitor (Anti-mmu-miR-24-3p ...
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bioRxiv - Synthetic Biology 2023Quote: ... or primary human cells were extracted using the Qiagen RNeasy Micro Kit (Qiagen). cDNA was produced using the SuperScript IV VILO cDNA Synthesis Kit (Thermo Fisher) ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA from human primary T cells was isolated using Gentra Puregene Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: RNA was extracted from human primary cells using AllPrep RNA/RNA/miRNA universal kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... mouse organ lysates and human primary cell supernatant samples were prepared in RNeasy Mini Kit (Qiagen) lysis buffer RLT (400μl) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from human primary immune cells using the RNeasy Plus Micro Kit (Qiagen). RNA integrity number (RIN ...
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bioRxiv - Systems Biology 2021Quote: RNA was extracted from male or female aortic valvular interstitial cells in culture using a RNeasy Micro Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: RNA was extracted from the cell pellets of human primary male and female PT cells using the RNAeasy Mini Kit (Qiagen). After quantifying RNA concentration in a Nanodrop instrument (Thermo) ...
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bioRxiv - Biochemistry 2022Quote: ... high molecular weight genomic DNA (gDNA) was purified from human primary T cells using the Blood & Cell Culture DNA Maxi Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: Genomic DNA from human primary CD4+/CD8+ T cells was isolated using the Gentra Puregene Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Spike-in Cel-miR-39 was added to cell conditioned media (CCM) and explanted swine aortic valves for normalization and quality control (219610, QIAGEN, Germany). All reactions were performed in technical triplicates ...
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bioRxiv - Pathology 2023Quote: ... Aortic tissue was frozen in liquid nitrogen and homogenized with lysis buffer (Qiagen, 64204) using an automatic bead homogenizer (MagNA Lyser ...
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bioRxiv - Bioengineering 2023Quote: Crushed whole abdominal aortic segments were immersed in RLT lysis buffer (Qiagen N.V., Venlo, Netherlands) and vortexed ...
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bioRxiv - Cell Biology 2022Quote: We isolated RNA from muscle stem or progenitor cells using the RNeasy Micro Kit (Qiagen). We reverse-transcribed cDNA from total mRNA from each sample using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary cells using Rneasy Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was extracted from sorted infected primary human hepatocytes using miRNeasy Micro Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Pulverized muscle tissue was lysed in RLT buffer (Qiagen) and RNA was extracted using the RNeasy® Mini Kit for fibrous tissue (Qiagen) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: RNA was extracted from aortic tissues (comprising the plaque and surrounding tissue) using an RNeasy mini kit (QIAGEN, Germany), and the RNA concentration was measured using a Nanodrop (2000C ...
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bioRxiv - Developmental Biology 2022Quote: Total RNAs were extracted from chick limbs or muscle cell cultures using the RNeasy mini kit from Qiagen. 500ng to 1µg RNAs were reverse-transcribed using the High Capacity Retrotranscription kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: Uninfected or infected primary human macrophages were subjected to RNA extraction using RNeasy Mini Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Total RNA was isolated from the aortic arch or descending aorta of adult mice using the RNeasy Fibrous Tissue Mini kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: Total RNA was isolated from the aortic arch or descending aorta of adult mice using the RNeasy Fibrous Tissue Mini kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Uninfected or infected primary human macrophages were subjected to RNA extraction using the RNeasy extraction kit (Qiagen). Two µg of RNA each was reverse transcribed with the iScript cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... Human cell motility RT2 profiler PCR Array (Qiagen) for control-MPC and Givi-MPC was performed ...
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bioRxiv - Cell Biology 2021Quote: ... Muscle samples were next lysed in ice-cold radioimmunoprecipitation assay (RIPA) buffer (400 μL/muscle) with protease and phosphatase inhibitor cocktail (Cell Signalling) using TissueLyser II (QIAGEN). Half of the lysate was mixed with scintillation cocktail for scintillation counting using Liquid Scintillation Analyzer (PerkinElmer) ...
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bioRxiv - Pathology 2021Quote: Total RNA from either snap-frozen muscles or cultured cells was isolated using an RNeasy Micro Kit (Qiagen, Hilden, Germany). The mRNA was reverse-transcribed into cDNA with PrimeScript RT Master Mix (TaKaRa ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was extracted from the muscles of the female adult golden pompano using the Blood & Cell Culture DNA Mini Kit (Qiagen). DNA concentrations and quality were measured using a NanoDrop 2000 (Thermo ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was collected from treated human or murine primary spinal cord astrocytes using an RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: MUSCLE alignments were performed on the CLC Genomics Workbench v12.0.3 (Qiagen) module Additional Alignments v1.9 for mutational frequency identification using the above mentioned ViPR sequences ...
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bioRxiv - Physiology 2019Quote: Soleus and plantaris muscles were homogenized in QIAzol (Qiagen, Hilden, Germany) and RNA was isolated using RNeasy Mini Kit (Qiagen ...
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bioRxiv - Physiology 2022Quote: ... The muscle sample was homogenized with TRIzol using PowerLyzer 24 (Qiagen), and RNA was isolated using Direct-zol RNA MiniPrep kit (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from freshly sorted 30,000 PDGFRα+ cells from LA/BC muscles using the RNeasy Plus Micro Kit (Qiagen, Cat# 74034) following the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: Snap frozen gastrocnemius muscles were pulverized and lysed in RLT buffer (Qiagen). RNA was extracted using the RNeasy® Mini Kit (Qiagen) ...
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bioRxiv - Physiology 2019Quote: RNA was isolated from frozen gastrocnemius muscles using an RNeasy kit (QIAGEN) and cDNA was generated using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Physiology 2022Quote: ... muscle was bead homogenized in Trizol using a TissueLyser II (Qiagen; 85300) and RNA extracted by addition of chloroform with centrifugation ...
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bioRxiv - Physiology 2023Quote: ... eWAT and skeletal muscle using the DNeasy Blood and Tissue Kit (Qiagen) following manufacturer’s instructions and treated with RNase A ...
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bioRxiv - Cancer Biology 2023Quote: ... muscle piece was lysed in 600 µL RLT Buffer (from Qiagen kit) with 1% β-Mercaptoethanol using beads tube from Precellys lysing kit for hard tissue (P000917-LYSK0-A ...
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bioRxiv - Genomics 2020Quote: ... and OA (from Wuxi Freshwater Fisheries Center in China) were extracted from muscle tissues using a Blood & Cell Culture DNA Midi Kit (Q13343, Qiagen, CA, USA). We also obtained genomic DNAs from ONJp with a YY genotype ...
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bioRxiv - Developmental Biology 2022Quote: RNA samples from freshly thawed human primary hepatocytes (SMC and AQL) or from PSC-hepatocytes were prepared with RNeasy plus micro kit (Qiagen), and 10-20 ng total RNA samples were used for constructing stranded RNA libraries with Swift RNA-seq library preparation kit and sequenced with an Illumina HiSeq 2500 sequencer with 50 base single end reads.
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: Bacterial and human cells were treated with RNAprotect cell or bacterial reagent (Qiagen, Germany) and stored at -80°C for up to 1 week ...
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bioRxiv - Cell Biology 2021Quote: ... and primary amniocyte cells using an RNeasy® Mini Kit (Qiagen) with DNAse I digestion as described in the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Primary endothelial cells were lysed in RNeasy Lysis buffer (Qiagen, 79216). Total mRNA was extracted from primary cells using the NucleoSpinR RNA (Macherey Nagel ...
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bioRxiv - Biochemistry 2021Quote: ... Muscles were thawed on ice and homogenized with a TissueLyser II (Qiagen Inc.) in 1 mL cold RIPA buffer (Thermo Fisher 89900 ...
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bioRxiv - Microbiology 2021Quote: ... muscles were freeze-dried then ground using a bead beater (TissueLyser II, Qiagen) while cultures and biofilms were only freeze-dried ...
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bioRxiv - Evolutionary Biology 2023Quote: ... longicorpa DNA isolated from muscle tissue using the Dneasy Blood &Tissue Kit (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2023Quote: ... genomic DNA was extracted from CD31+ cells isolated from skeletal muscle and liver from AHRfl/fl and AHRecKO mice using Qiagen DNeasy kit (Qiagen, Cat. No. 69504) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: The Human Cancer Stem Cells RT² Profiler PCR Array from Qiagen was used to profile the expression of 84 genes linked to cancer stem cells (CSCs ...
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bioRxiv - Cell Biology 2022Quote: ... for siRNAs (human YTHDF1: QIAGEN SI00764715, human YTHDF2: QIAGEN SI00764757, human YTHDF3: QIAGEN SI04133339, human METTL3: QIAGEN SI05020414 and human DDX6 ...
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bioRxiv - Neuroscience 2020Quote: Primary microglia or BV-2 cells were collected in RLT buffer (QIAGEN). RNA was isolated using RNEasy Micro or Mini Kit ...