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Citations for Qiagen :
1 - 50 of 833 citations for Prestained Protein Peptide Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The peptide-coupled proteins were separated from uncoupled proteins using Ni-NTA Agarose (Qiagen).
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bioRxiv - Biochemistry 2023Quote: ... The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein purification was performed using Ni+2-NTA agarose affinity chromatography according to standard protocol (Qiagen).
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bioRxiv - Immunology 2022Quote: ... Protein expression was performed using standard protocols and purification was performed using Ni-NTA Agarose (Qiagen). Recombinant RelMtb protein (87 kDa ...
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bioRxiv - Bioengineering 2020Quote: ... Standard Miniprep (Qiagen) protocol was performed in order to collect DNA ...
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bioRxiv - Biophysics 2022Quote: ... using standard conditions (Qiagen). Protein purified through Ni-NTA chromatography was subjected to further (polishing-step ...
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bioRxiv - Biophysics 2021Quote: ... The target protein was eluted by 0.2 mg/ml FLAG peptide and load to Ni-NTA affinity gel (Qiagen). After washed by Lysis Buffer supplied with 0.02% GDN and 0.004% CHS ...
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bioRxiv - Biophysics 2021Quote: ... in standard crystallization screens (QIAgen) using a mosquito protein crystallisation robot (sptlabtech) ...
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bioRxiv - Biochemistry 2023Quote: ... as per standard protocols (Qiagen). The eluted protein was dialyzed in 50 mM Tris buffer of pH 8.0 and electrophoretically examined on SDS PAGE using a Bio-Rad Tetrad apparatus ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled protein in the soluble fraction was purified using Immobilized Metal Affinity Chromatography (IMAC) using standard methods (QIagen Ni-NTA resin). The purified protein was then concentrated to 2 mL and purified by FPLC sizeexclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: The recombinant His-TvFACPα full-length protein was produced and purified by a standard protocol as suggested by the supplier (QIAGEN, Hilden, Germany) (48 ...
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Transcriptomic and Cellular Decoding of Regional Brain Vulnerability to Neurodevelopmental DisordersbioRxiv - Neuroscience 2019Quote: ... RNA was extracted by standard methods (Qiagen), and qRT-PCR was performed using the Fluidigm platform ...
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bioRxiv - Neuroscience 2019Quote: ... and utilizing standard consumables (Qiagen, Manchester, UK). PCR reactions were performed using 5μl cDNA mix and 200nM custom-designed primers (Table 1 ...
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bioRxiv - Neuroscience 2020Quote: ... Those standards were generated by mixing commercially available unmethylated and hypermethylated DNA standards (EpiTect PCR Control DNA Set, Qiagen) resulting in 10 ng of total bisulfite-converted DNA ...
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bioRxiv - Immunology 2020Quote: ... b is the intercept-y of the standard curve and m is the slope of the standard curve (QIAGEN, 2014). Subsequently ...
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bioRxiv - Developmental Biology 2021Quote: ... coli and purified using a standard protocol (QIAGEN) at a temperature lower or equal to 12 °C to obtain a near 100 % supercoiled DNA ...
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bioRxiv - Genomics 2021Quote: ... according to standard protocol on the QiaCube (Qiagen) RNA-sequencing (RNA-seq ...
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bioRxiv - Genomics 2023Quote: ... following the manufacturer’s standard protocol (Qiagen, Valencia, CA), and the concentration was measured using a QUBIT 3 fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: Standard protocols from the miRNeasy Mini kit (Qiagen) was used to extract total RNA with the inclusion of on-column genomic DNA digestion step using the RNase-free DNase Kit (Qaigen) ...
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bioRxiv - Genetics 2020Quote: ... according to the standard operating procedure provided by QIAGEN. The extracted DNA was detected by NanoDrop™ One UV-Vis spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmids were prepared for injection using standard miniprep extraction (Qiagen) and submitted for injection (Microinjection service ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue RNA was extracted using standard protocol of Qiazol (Qiagen) tissue lysis by TissueLyser (2min ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated through standard methods following manufacturer’s instructions (QIAGEN), and reverse transcribed with Superscript III (Life Technology) ...
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bioRxiv - Genomics 2019Quote: ... PCR reaction mixtures contained 10x Standard Taq Reaction buffer (Qiagen), 2 mM MgCl2 ...
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bioRxiv - Genomics 2020Quote: Performances of the approaches (gold standard, pFACS-RNAseq and QIAGEN) in our study were first evaluated by mapping statistics ...
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bioRxiv - Cancer Biology 2020Quote: Tissue RNA was extracted using standard protocol of Qiazol (Qiagen) tissue lysis by TissueLyser (2min ...
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bioRxiv - Cancer Biology 2020Quote: RNA isolation was performed according to standard procedure (Qiagen kit). qRT-PCR was carried out with SYBR Green I (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted using the standard DNeasy PowerSoil Kit protocol (Qiagen). Extracted DNA was PCR amplified with barcoded primers targeting the V4 region of 16S rRNA gene according to the Earth Microbiome Project 16S Illumina Amplicon protocol with the 515F:806R primer constructs (113) ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was purified using a standard PCR-clean-up column (QiAGEN). 2 μl of purified ChIPed DNA was used in the subsequent qPCR reaction.
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bioRxiv - Evolutionary Biology 2019Quote: ... using the QIAGEN Genomic-tip 20/G standard protocol (Qiagen, UK). All samples were sequenced at the University of Liverpool from Illumina TruSeq Nano libraries with 350bp inserts using 125bp paired-end reads on an Illumina HiSeq2500 platform ...
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bioRxiv - Biochemistry 2023Quote: ... and IMAC affinity chromatography was performed according to standard protocols (Qiagen). The supernatant of TonAmyGT and Chimera 4 were flash dialyzed against native lysis buffer and then subjected to Ni-NTA IMAC affinity chromatography ...
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bioRxiv - Genomics 2022Quote: ... Non-methylated (0%) and fully methylated (100%) standards (Qiagen, Hilden, Germany) were used as controls to examine the consistency of methylation levels across all batches ...
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bioRxiv - Microbiology 2024Quote: ... We followed the standard protocol of the kit DNeasy PowerWater (Qiagen) for the water sample extractions ...
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bioRxiv - Immunology 2021Quote: ... and an undisclosed peptide pool inducing CD8+ T lymphocyte stimulation (Qiagen, 2017); rmsHBHA which tubes contain recombinant M ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmid DNA was isolated from mutant clones using a standard protocol (QIAGEN). After paired-end Sanger sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using standard column-based purification kit (QIAGEN RNeasy Kit, Cat. No. 74004). DNase treatment was applied during the purification to remove any potential DNA contamination ...
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bioRxiv - Systems Biology 2019Quote: ... was used for DNA extraction (DNeasy Plant Mini Kit, Qiagen; standard protocol). Nucleotide concentrations were verified with NanodropONE (Thermofisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... DNA was then extracted following the standard DNeasy PowerSoil Pro kit (Qiagen) protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was isolated with QIAamp DNA mini kit using standard protocols (QIAGEN). DNA fragments of 400–900 bp were generated by sonication and subsequently ligated to adaptors ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... After purification from standard agarose gels (QIAquick Gel Extraction Kit, Qiagen 28704), PCR products were sequenced with either the Fw and/or Rw primer listed in Table S1.
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bioRxiv - Microbiology 2020Quote: ... coli 10-beta cloning strain were purified using the standard QIAprep protocol (QIAGEN), then delivered to the Pseudomonas strain via previously published electroporation83 or conjugation84 protocols ...
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bioRxiv - Cancer Biology 2022Quote: ... 16S rDNA was isolated following the standard protocol and (QIAamp PowerFecalPro Kit) (Qiagen). Isolated samples were sent to Miami University of Ohio on dry ice for 16S V4 rDNA processing ...
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bioRxiv - Bioengineering 2019Quote: ... Total RNA extraction was performed using a standard kit (RNeasy mini kit, Qiagen). RNA concentration was quantified (ND-1000 ...
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bioRxiv - Systems Biology 2021Quote: ... Amplified plasmid was recovered using a standard mini prep kit (Qiagen, Hilden, Germany) and sent for sequencing.
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bioRxiv - Immunology 2021Quote: RNA from positive cultures was extracted by using standard procedures (RNeasy minikit; QIAGEN), and the genes encoding Ig VHDJH and VLJL rearrangements were amplified by RT and nested PCR without cloning by use of a previously reported method (Liao et al. ...
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bioRxiv - Evolutionary Biology 2019Quote: We extracted total DNA from frozen tissues using standard methods and kits (Qiagen, Valencia ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The RNA was extracted using a standard QIAzol Lysis Reagent (QIAGEN, Cat # 79306) procedure ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from cells following the standard RNeasy kit protocol (Qiagen; 74134), and concentrations were measured using a NanoDrop (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... The His-tagged fusion peptide was purified using nickel–nitrilotriacetic acid (Ni–NTA, Qiagen) resin affinity chromatography and by C18 reverse-phase chromatography (Sep-Pak® Waters ...
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bioRxiv - Cell Biology 2021Quote: ... then lysed directly in buffer RLT according to the standard RNeasy kit protocol (Qiagen). Lysate was homogenized with the QIAshredder columns (Qiagen ...