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Citations for Qiagen :
1 - 50 of 2122 citations for Pig Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: MCF10A-ER-src cells were transiently transfected with the CAV1 overexpressing plasmid using Effectene transfection reagent (301425, Qiagen). One day before transfection ...
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bioRxiv - Cell Biology 2019Quote: ... accordingly to manufacturer’s instructions using non-targeting siRNA (siCtrl; 5’-AATTCTCCGAACGTGTCACGT-3’) and siRNA targeting Cav1 (SI00299635 and SI00299628) from Qiagen, or using pre-miR-NC (negative control ...
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bioRxiv - Genomics 2020Quote: DNA was isolated from either hair roots (living pigs) or spleen (slaughtered pigs) using DNeasy blood and tissue kit (Qiagen). A PCR test was established consisting of a FAM-labelled forward primer (Fw-DNAH17 ...
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bioRxiv - Genomics 2022Quote: Total RNA was isolated from day 15 fetal brain of WT and Cav1-null mice as well as from week 70 old Cav1-null mice using an AllPrep DNA/RNA Mini Kit (Qiagen, Cat No./ID: 80204) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... PIGS-B4GALT5-B4GALT6 TKO+B4GALT5-D300A cells using the RNeasy Mini kit (QIAGEN). Each RNA was then transcribed to cDNA using the SuperScript VILO cDNA Synthesis kit (Thermo Fisher) ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was isolated from pig ear punches using a DNA kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... we utilized the RT2 Profiler PCR Array - Pig Fibrosis (Qiagen, Gene GlobeID: PASS-120ZD). RNA samples were isolated and purified as previously described ...
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bioRxiv - Neuroscience 2021Quote: ... and loaded into the Pig Inflammatory Cytokines & Receptors RT2 ProfilerTM PCR Array (Qiagen, Hilden, Germany). The qPCR reactions were performed using a Qiagen Rotor-Gene Q real-time cycler ...
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bioRxiv - Microbiology 2020Quote: ... blood and organs of the pigs using the DNeasy Blood &Tissue kit (Qiagen GmbH, Hilden, Germany). DNA was quantified using a NanoDrop ND-1000 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2023Quote: ... susceptible and normal pigs were isolated from PBMC using RNeasy Plus Universal Mini Kit (QIAGEN, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The YB-1 (1-180) protein fragment was purified following the manufacturer’s recommendations (Qiagen). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was isolated from pig fat or liver tissues by TRIzol and RNeasy Mini Kit (QIAGEN). Then RNA samples were reverse transcribed to cDNA by M-MLV reverse transcription kit (Promega) ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extract (1 mg) was incubated with His6-RabD2c (1 μg) and Ni-NTA Agarose (QIAGEN) for 1 h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... DNA was isolated from the collected vaginal swab and DRG of guinea pigs by using DNeasy blood and tissue kits (Qiagen). The presence and quantification of HSV- 2 was done by real-time PCR (StepOnePlus Real-Time PCR System ...
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bioRxiv - Immunology 2023Quote: ... DNA was isolated from the collected vaginal swab and DRG of guinea pigs by using DNeasy blood and tissue kits (Qiagen). The presence of HSV-2 DNA was quantified by real-time PCR (StepOnePlus Real-Time PCR System ...
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bioRxiv - Immunology 2022Quote: ... The cleaved protein was passed over a 1 mL Ni-NTA agarose (Qiagen) gravity column to remove TEV protease ...
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bioRxiv - Molecular Biology 2023Quote: The eluted protein was mixed with 1 ml Strep-Tactin Superflow agarose (Qiagen) and incubated at 4 °C for 30 min ...
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bioRxiv - Microbiology 2023Quote: Blood samples of all categories of pigs were subjected to extract the genomic DNA using DNeasy Blood & Tissue Kits (QIAGEN, Germany) manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: RNAseq: mRNA was extracted from the iPSCs of the pig-tailed macaque using RNeasy Mini Kit extraction kit (Qiagen Inc. Valencia CA). Following cDNA synthesis ...
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bioRxiv - Genomics 2022Quote: Viral RNA of JEV was extracted from blood and tissue samples collected from pigs and mosquito samples (Mosquitoes were pooled as approx. 30 mosquitoes/pool) using QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany) as per manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Profiling of TGF-β targets was performed using the RT2 Profiler™ PCR Array Pig TGF-β Signaling Targets kit from Qiagen following manufacturers’ instructions.
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bioRxiv - Biophysics 2019Quote: ... proteins were purified by batch Ni-NTA bead purification (1 mL slurry/1L culture; Qiagen) and further purified by a Superdex 200 16/60 column (GE Healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... Bound STAT3 proteins were then labeled with Alexa488-conjugated anti-6 × His antibodies (1 hr incubation, 1:20 dilution, Qiagen 35310). The array was washed and scanned using a GenePix 4400A scanner (Molecular Devices ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...
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bioRxiv - Developmental Biology 2022Quote: Microbial genomic DNA was extracted from the pigs colonic digesta samples and the sows faeces using a QIAamp PowerFecal Pro DNA stool kit (Qiagen, West Sussex, UK) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from the tissues of warthogs and Kenyan domestic pigs using the DNA MagAttract HMW DNA Kit (Qiagen, Germantown, MD, USA) for 10× genomics according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... followed by protein precipitation with the Protein Precipitation Solution (Qiagen) for 10 min at -20 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant proteins were purified on Ni-NTA columns (1 ml Ni-NTA Agarose; Qiagen Cat # 30210), washed with 20 ml hexokinase buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... The purified recombinant proteins were analyzed by western-blot with anti-His antibody (1:2000 dilution) (Qiagen) followed by HRP-labeled anti-mouse IgG (1:50000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... Protein extraction was conducted using a Qproteome Bacterial Protein Prep Kit (Qiagen)‡ ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein isolation with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen).
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bioRxiv - Cancer Biology 2023Quote: ... Protein-protein interaction networks were built using Ingenuity Pathway Analysis (IPA) (Qiagen).
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bioRxiv - Genetics 2021Quote: ... Protein Precipitation Solution (Qiagen) was added at 0.33x and mixed well ...
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bioRxiv - Cancer Biology 2022Quote: Whole tissue protein was extracted by Qproteome Mammalian Protein Prep Kit (Qiagen, 37901) according to the manufacture’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein were extracted using the AllPrep DNA/RNA/Protein kit (Qiagen, #47054). Sample concentrations were measured with Qubit high sensitivity dsDNA and RNA platform ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated by adding 100 μL of a protein precipitation solution (Qiagen). Samples were centrifuged for 5 min at 13000 rpm ...
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bioRxiv - Plant Biology 2023Quote: ... All protein sequences (for accession numbers see Supplementary Table 1) were aligned using CLC Genomics Workbench v20.0.4 (Qiagen) and the phylogenetic tree was constructed with the neighbor-joining method using 1000 bootstrap replicates ...
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bioRxiv - Genomics 2020Quote: ... Protein was precipitated by adding 200 µL of ice-cold Protein Precipitation Solution (Qiagen), gentle mixing and incubation on ice for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The peptide-coupled proteins were separated from uncoupled proteins using Ni-NTA Agarose (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... N-terminal His-tagged proteins were purified using a QIAexpress protein purification system (Qiagen), as previously described47.
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bioRxiv - Neuroscience 2020Quote: ... Total protein was extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen #80004) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein was purified by the AllPrep DNA/RNA/Protein Mini Kit (Qiagen: # 80004). Corresponding protein expression levels in the cells of different groups were detected by western blot using the following antibodies ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested for total protein using a Qproteome Mammalian Protein Prep Kit (Qiagen). Total protein was quantified using a PierceTM BCA Protein Assay Kit (Cat ...
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bioRxiv - Developmental Biology 2022Quote: ... 333µl Protein Precipitation Solution (Qiagen) was added and samples were vortexed vigorously for 20 seconds at high speed ...
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bioRxiv - Cell Biology 2023Quote: ... Protein Precipitation Solution (Qiagen #158910), DNA Hydration Solution (Qiagen #158914 ...
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bioRxiv - Biochemistry 2021Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Molecular Biology 2021Quote: The solubilized protein was incubated for 1 hour with washed and pre-equilibrated Strep-Tactin Superflow plus resin (Qiagen). The resin was then loaded into an Econo-Column Chromatography Column (Biorad ...
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bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...