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Citations for Qiagen :
1 - 50 of 10000+ citations for Mouse WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... penicillin and streptomycin (R10) containing 2.7 Kunitz units/ml of DNAse (Qiagen). Cells were stained in BD Horizon Brilliant Stain Buffer (BD ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.5 Kunitz units DNase I (Qiagen) was added to the reaction and allowed to sit for an additional 15 min to remove the template DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 Kunitz unit DNase I (Qiagen) was added to the reaction and allowed to sit for an additional 15 min to digest the template DNA ...
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bioRxiv - Biochemistry 2024Quote: ... 0.05 Kunitz units/µL RNase-free DNase (QIAGEN)) ...
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bioRxiv - Cell Biology 2020Quote: The validated Alx1DN (N-terminal portion of protein product containing homeodomain and nuclear localization domains) clones in pCS2+8 were purified via miniprep (Qiagen) alongside a control (C-terminal portion containing transactivation domain) ...
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bioRxiv - Microbiology 2020Quote: ... samples were treated with RNase-Free DNase I (14 Kunitz units/rxn, Qiagen) for 30 min at RT ...
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bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Microbiology 2019Quote: ... DNase digestions were carried out on the column by adding 82 units of Kunitz enzyme (Qiagen) with incubation at room temperature for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... following the manufacturer’s instructions and samples treated with RNase-Free DNaseI (14 Kunitz units/rxn, Qiagen) for 30 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... remaining cellular DNA was digested using 0.0164 Kunitz Units/µL DNase I in Buffer RDD (79254, Qiagen), RNA digestion was prevented by utilizing 0.533 U/µL RNasin® ribonuclease inhibitor (N2511 ...
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bioRxiv - Microbiology 2019Quote: ... containing 2 ml microcentrifuge tubes (QIAGEN) in which samples were placed ...
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bioRxiv - Neuroscience 2021Quote: Tissue-gel samples were equilibrated in DNase buffer (750 ml) for 30 min and then incubated with RNase-free DNase1 (2.7 Kunitz units/µl, 50 µl) (Qiagen) in DNase buffer (450 µl ...
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bioRxiv - Biophysics 2021Quote: ... The correct band with the GRB2-SH3 domain was purified using the MinElute Gel Extraction Kit (QIAGEN) and the sample was quantified using a Qubit fluorometer to be cloned into the yeast assay plasmids by high efficiency temperature-cycle ligation64.
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bioRxiv - Biochemistry 2022Quote: Approximately 25 mg of frozen tissues were transferred to 2 mL Eppendorf Protein Lobind tubes containing one 5 mm stainless steel bead (cat# 69989, Qiagen) and 500 µL of lysis buffer consisting of 5% SDS in 50 mM TEAB with protease inhibitors cocktail (cat# A32953 ...
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bioRxiv - Biochemistry 2020Quote: ... low melting point agarose gel and the approximately 700 base pair band corresponding to the heavy chain only immunoglobulin was extracted and purified using QIAquick Gel Extraction Kit (Qiagen). A secondary amplification of the VHH domain was performed using modified primers (VHH-Esp-For ...
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bioRxiv - Molecular Biology 2021Quote: ... Approximately 700 base pair bands corresponding to the heavy-chain only immunoglobulin were extracted (QIAquick Gel Extraction Kit: Qiagen K.K., Tokyo, Japan). Nested PCR was performed to amplify coding genes of VHH domains using the VHH-PstI-For and VHH-BstEII-Rev primers and subcloned into the pMES4 phagemid vector5 ...
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bioRxiv - Cancer Biology 2023Quote: ... and lower bands corresponding to the heavy-chain only immunoglobulin were extracted using the QIAquick Gel Extraction Kit (Qiagen K.K., Tokyo, Japan). Subsequent nested PCR was performed to amplify coding genes of VHH domains using the VHH-PstI-For and VHH-BstEII-Rev primers and subcloned into the pMES4 phagemid vector36 ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted from 1 ml cell suspensions of each isolate standardised to A600 0.65 (containing approximately 2 x 108 cells) using the RNeasy Mini kit (Qiagen) with enzymatic lysis and Proteinase K digestion ...
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bioRxiv - Neuroscience 2019Quote: ... and cell pellets were lysed using RLT+ buffer containing 2-Mercaptoethanol using the Qiagen RNeasy+ Micro Kit (Qiagen #74034), following manufacturer’s suggested protocol for RNA isolation ...
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bioRxiv - Biophysics 2021Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the two assay plasmids by temperature-cycle ligation.
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bioRxiv - Biophysics 2022Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the AbundancePCA plasmid and BindingPCA plasmids by temperature-cycle ligation ...
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bioRxiv - Genomics 2022Quote: ... 32 μl Master Mix containing 30 μl MDA reaction buffer and 2 μl Phi29 polymerase (REPLI-g UltraFast Mini Kit; Qiagen) were added ...
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted directly into 2-Mercaptoethanol-containing RLT buffer and RNA was extracted using a RNeasy Mini kit (Qiagen). The cell populations used for RNA sequencing are summarized in Table S1 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... containing 1 × Master Mix (Qiagen Multiplex Kit), 0.4 μg/μL of BSA ...
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bioRxiv - Synthetic Biology 2019Quote: ... The final domain-insertion library was obtained by purifying plasmids from this cell slurry using a QIAprep Spin Miniprep Kit (Qiagen, Inc.).
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bioRxiv - Genetics 2021Quote: Multi-array ELISA was performed using the Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... the supernatants containing denatured proteins were mixed with Ni-NTA resin (Qiagen) and incubated for 60 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: SacSm-containing protein sample was loaded on a Ni-NTA Agarose (Qiagen) column equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Systems Biology 2021Quote: ... 2 mL samples were mixed into glass tubes containing 2 volumes of RNAprotect bacteria reagent solution (Qiagen). After mixing and incubating for 5 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... containing a Lysis buffer ((RNeasy Micro Kit (Qiagen)) ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and proteins were extracted from fresh or snap-frozen FACS-sorted 2-5 million splenic B cells using AllPrep DNA/RNA/Protein Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... the DNA was extracted from 100 mg mouse fecal samples (9-week-old, 2 weeks after final oral gavage treatment) using QIAamp PowerFecal Pro DNA kit (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2020Quote: ... including miRNA was isolated from brains of E11 mouse fetuses (3 biological replicates) at 2 h after irradiation using the miRNeasy Mini Kit (Qiagen). RNA was subsequently processed for hybridization to GeneChip miRNA 4.0 microarrays (Affymetrix ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA from cultured mouse neuro 2a cells was extracted using AllPrep DNA/RNA/Protein Mini Kit (80004, Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Protein extraction was conducted using a Qproteome Bacterial Protein Prep Kit (Qiagen)‡ ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein isolation with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen).
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bioRxiv - Microbiology 2022Quote: ... 2) the DNeasy PowerWater Kit (Qiagen), which is optimized for the isolation of genomic DNA from filtered water samples ...
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bioRxiv - Molecular Biology 2021Quote: ... containing 700μl Buffer RLT (RNeasy MinElute Cleanup Kit, Qiagen). 500 μl of 100% ethanol was added to the RNA solution ...
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bioRxiv - Developmental Biology 2019Quote: ... containing β-mercaptoethanol and the RNeasy Mini kit (Qiagen) followed by in-column DNase I treatment to remove genomic DNA contamination ...
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bioRxiv - Cell Biology 2021Quote: AllPrep DNA/RNA/Protein Mini Kits (Qiagen) were used to extract total proteins from UVA-exposed and non-exposed HTEpC from all experiments ...
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bioRxiv - Microbiology 2022Quote: ... hACE2 expression or SARS-CoV-2 nucleocapsid (N) gene in individual mouse organs was determined using QuantiNova SYBR Green PCR kit (Qiagen #208052) in combination of 500 nM of hACE2 gene specific primer set (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2022Quote: Whole tissue protein was extracted by Qproteome Mammalian Protein Prep Kit (Qiagen, 37901) according to the manufacture’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein were extracted using the AllPrep DNA/RNA/Protein kit (Qiagen, #47054). Sample concentrations were measured with Qubit high sensitivity dsDNA and RNA platform ...
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The gut hormone Allatostatin C regulates food intake and metabolic homeostasis under nutrient stressbioRxiv - Physiology 2020Quote: ... were homogenized in 2-mL tubes containing lysis buffer plus 1% 2-mercaptoethanol using a TissueLyser LT bead mill (Qiagen) and 5-mm stainless-steel beads (Qiagen #69989 ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted and purified from 20 mg (2 pellets) of stool from each mouse using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germantown, MD). The concentration of DNA in samples was determined by spectrophotometry ...
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bioRxiv - Biochemistry 2023Quote: Mouse liver RNA was extracted from 10 mg (± 2 mg) of preserved tissue using RNEasy kits and QIAshredders (QIAGEN; Germantown, MD, USA). Aliquots of RNA from each sample were assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Caps contained guanidine isothiocyanate-containing buffer (RNeasy Mini Kit, Qiagen) and 1% β-mercaptoethanol (AppliChem GmbH) ...
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bioRxiv - Neuroscience 2020Quote: ... Total protein was extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen #80004) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein was purified by the AllPrep DNA/RNA/Protein Mini Kit (Qiagen: # 80004). Corresponding protein expression levels in the cells of different groups were detected by western blot using the following antibodies ...