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Citations for Qiagen :
1 - 50 of 1933 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... shRNA constructs were obtained from QIAGEN (SureSilencing Akt1 shRNA plasmids) or Origene (HuSH Myh9 shRNA constructs).
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bioRxiv - Neuroscience 2019Quote: Four Arc shRNA plasmids (SureSilencing, Qiagen) were transfected into neuronal cultures using Lipofectamine 2000 (Qiagen) ...
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bioRxiv - Immunology 2023Quote: ... and the shRNA plasmid using SuperFect (Qiagen). T cells were transduced by centrifugation and selected with puromycin.
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bioRxiv - Cell Biology 2022Quote: Alox12b and Tgm1 shRNA knockdown in rat epidermal keratinocytes using SureSilencing shRNA plasmids (Qiagen) has been previously described (O’Shaughnessy et al ...
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bioRxiv - Cancer Biology 2022Quote: ... ShRNA plasmids were purified with QIAprep Spin Miniprep Kit (#27106, Qiagen) after overnight incubation with E-coli bacteria ...
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bioRxiv - Neuroscience 2022Quote: ... Rai14 shRNA or scrambled shRNA sequence in pLL3.7-EGFP vectors were purified by using EndoFree plasmid maxi kit (Qiagen, Germantown, MD, USA). Each DNA solution (2.0 μg/μL ...
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bioRxiv - Cancer Biology 2020Quote: ... 1×106 HEK-293T cells were transfected with 2 μg of plasmid DNA containing each shRNA along with packaging plasmids pMD2.g and psPax2 using Effectene® Transfection Reagent (QIAGEN) to yield 5 mL of supernatant containing lentivirus ...
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bioRxiv - Cancer Biology 2020Quote: H23 cells were stably transfected with PCK2 shRNA or non-silencing control shRNA (Qiagen, Hilden, Germany). Puromycin-selection and generation of monoclonal subcultures was performed as described (Leithner et al ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 cells were transfected with 1:1:2 μg of packaging plasmids versus shRNA hairpins on the pLKO.1 vector using Effectene transfection reagent (Qiagen) 48 h prior to harvesting supernatants ...
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bioRxiv - Molecular Biology 2023Quote: ... shRNA-Cacna2d2 using the miRNeasy Mini Kit (Qiagen, Germantown, MD) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The recombinant plasmids for mouse vaccination were prepared using an EndoFree Plasmid Purification Kit (Cat# 12391, Qiagen, Hilden, USA).
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bioRxiv - Developmental Biology 2023Quote: ... The control shRNA (shCtrl) was generated from AllStars Negative Control siRNA (Qiagen), which does not recognize mammalian RNAs ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: RNA was isolated from shRNA-transduced BMDCs using RNeasy kit (Qiagen, Germantown, MD). 1 μg RNA was reverse transcribed to cDNA using TaqMan™ Reverse Transcription Kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were isolated (Qiagen Plasmid Mini Kit) and sequenced (Eurofins Genomics ...
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bioRxiv - Immunology 2022Quote: ... 97-mer shRNA oligonucleotides were synthesized (IDT) and 4 picomoles were amplified with HotStarTaq polymerase (Qiagen#203207) using the primers miR-E-fw (5’-TGAACTCGAGAAGGTATATTGCTGTTGACAGTGAGCG-3’ ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was collected 4 days post lentiviral shRNA transduction using RNeasy Plus Mini Kit (Qiagen #74134). Lentiviral transduction and RNA extraction were performed in triplicates ...
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bioRxiv - Biochemistry 2020Quote: ... Purified plasmid (Plasmid Plus Midi Kit, QIAGEN, Germany) was mixed with selection plasmid pCoBlast (1:10 ...
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bioRxiv - Systems Biology 2022Quote: ... Plasmids were extracted using Plasmid Miniprep Kit (Qiagen). gDNA was extracted using DNeasy Blood & Tissue Kit (Qiagen) ...
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bioRxiv - Biochemistry 2022Quote: ... pRL02 plasmid was isolated (Qiagen plasmid isolation kit) from DH5α E ...
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bioRxiv - Genetics 2023Quote: ... Plasmid was collected using QIAquick Plasmid Kit (QIAGEN) and named pUB2441 (3V5-αGFP) ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA was then purified (QIAGEN Plasmid Kit) and sequenced (Genewiz).
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were purified by Plasmid Maxi Kit (Qiagen) according to the manufacture’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... All cells treated with a particular shRNA were pooled for RNA isolation using the RNeasy Mini kit (Qiagen). DNaseI (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed to extract the shRNA sequence and column purification with the QIAprep PCR Purification Kit (Qiagen #28104) was performed to concentrate the digested SGEN plasmid ...
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bioRxiv - Neuroscience 2021Quote: ... plasmids were purified using Plasmid Plus Purification Kit (Qiagen) and injected into w ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were purified using Plasmid Plus Maxi kits (Qiagen), quantified (Nanodrop 1000 spectrophotometer ...
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bioRxiv - Systems Biology 2019Quote: ... and plasmids extracted using Plasmid Mini Kit (Qiagen, UK). Plasmids were indexed and stored in 96 well plates and at −20 °C ...
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bioRxiv - Neuroscience 2021Quote: ... The plasmid was purified with Plasmid Midi Kit (QIAGEN) and digested with restriction enzyme Spe I ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmid Plus Midi or Plasmid Maxi Kit (all Qiagen).
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bioRxiv - Microbiology 2021Quote: ... Each plasmid DNA was isolated by plasmid purification (Qiagen). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmids were isolated using the Plasmid Midi Kit (Qiagen).
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids were transformed into DH5α electrocompetent cells for cloning and plasmid purification (Qiagen Plasmid Midi Kit) for sequencing and cell-free reaction.
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bioRxiv - Cancer Biology 2023Quote: Lineage tracing plasmids and envelope-expressing plasmids were prepared using a plasmid maxi kit (QIAGEN cat. 12162). To produce lentivirus ...
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bioRxiv - Cancer Biology 2020Quote: ... The lentiviral plasmid was prepared using plasmid midiprep kit (Qiagen) for lentivirus preparation.
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bioRxiv - Genomics 2020Quote: ... The plasmid DNA was produced with Plasmid Midi Kit (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... and plasmid extraction was performed using Qiagen Plasmid Maxikit (Qiagen) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2020Quote: ... These plasmids were purified with Plasmid DNA Purification Minipreps (Qiagen), used as a template in cycle sequencing reactions and run on 4% denaturing polyacrylamide gels in an ABI 377 automated sequencer (Applied Biosystems ...
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bioRxiv - Genomics 2022Quote: ... Plasmids were extracted with the HiSpeed Plasmid Maxi Kit (Qiagen).
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bioRxiv - Neuroscience 2022Quote: ... Plasmids were extracted with the QIAfilter Plasmid Midi Kit (QIAGEN) and then linearized using an appropriate restriction enzyme (Takara) ...
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bioRxiv - Cell Biology 2022Quote: ... and plasmids purified using Plasmid Miniprep or Midiprep Kits (Qiagen) or a PureLink HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid DNA was purified using Plasmid Plus Midi Kit (Qiagen) or NucleoBond Xtra Midi Kit (Machery Nagel) ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid was purified using plasmid plus giga kits (Qiagen) following standard protocol ...
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bioRxiv - Microbiology 2019Quote: ... and plasmids were extracted using QIAGEN Plasmid Midi Kit (QIAGEN). Plasmid sequencing was performed using Illumina MiSeq platform (2 x 301 bp ...
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bioRxiv - Systems Biology 2021Quote: ... Plasmid DNA was purified with the QIAprep Plasmid MiniSpin (QIAGEN) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were purified using the EndoFree Plasmid Maxi Kit (Qiagen). Presence of the mutation at the desired site without additional off-target effects was evaluated by Sanger sequencing (Table S5) ...
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bioRxiv - Immunology 2022Quote: ... Plasmid DNA was purified using HiSpeed Plasmid Midi Kit (Qiagen). Plasmid DNA was linearized by MluI ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmid DNA was isolated using EndoFree Plasmid Maxi Kit (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Evolutionary Biology 2023Quote: Sea robin T1r plasmids were purified (Qiagen Plasmid Mini Kit) and in vitro responses were measured using a luminescence cell-based assay16,17 ...
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bioRxiv - Biochemistry 2024Quote: Plasmid DNA was prepared using a Plasmid Maxi Kit (Qiagen). EXPI293 cells (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were isolated using a Plasmid Mini Kit (Qiagen), sequenced using Sanger sequencing (Eurofins Genomics ...