Labshake search
Citations for Qiagen :
1 - 50 of 10000+ citations for Medium Kit without Serum and without Growth Factors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... total RNA was isolated from the study strains following overnight growth in media with and without NaHCO3 supplementation using an RNeasy Kit (Qiagen, Valencia, CA) (66) ...
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bioRxiv - Immunology 2021Quote: ... and small intestine without contents using the RNeasy Mini Kit (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and were then incubated with or without triton-x (1% v/v) and with or without RNase-A (Qiagen #19101 ...
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bioRxiv - Microbiology 2023Quote: Three individual colonies of WT and Pcu-shc::Δshc growth with and without 25 µM cumate were cultured to early exponential phase and total RNAs were extracted by Qiagen RNeasy Mini Kit as described previously (44) ...
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bioRxiv - Cell Biology 2023Quote: ... Drosophila S2 cells were cultured in Excel media without fetal bovine serum (FBS) and co-transfected with Effectene (Qiagen). The expression of the copper-inducible transgenes was induced by adding CuSO4 (700 µM ...
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bioRxiv - Microbiology 2023Quote: DNA was isolated from microbiome samples (with and without PMA) using the DNeasy PowerSoil Kit (Qiagen) (Figures 2-5 ...
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bioRxiv - Microbiology 2024Quote: ... Soil supernatants without pre-enrichment were extracted using the DNeasy PowerSoil Pro kit (QIAGEN, Hilden, Germany), while supernatants from enriched soil/water samples and rectal swabs were extracted using the High Pure PCR Template Preparation kit (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated in a 50 µL reaction with or without triton-x (1% v/v) and with or without proteinase K (Qiagen #19131, final concentration 20 µg/mL) for 15 minutes at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... 110.25 uL XVIVO depleted (without MCSF) and 11 uL HiPerfect (Qiagen) were gently mixed in a tube and incubated at room temperature for 15 to 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... The master mix without primers was purchased from Qiagen (Manchester, UK) and primers were synthesised from Primer Design (Southampton ...
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bioRxiv - Genomics 2021Quote: ... The adaptor-ligated DNA were cleaned without size selection using the MinElute® PCR Purification Kit (QIAGEN, Germany), following the instructions provided by the manufacturer ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was isolated from roots treated with or without bleomycin using an RNeasy Plant Mini Kit (Qiagen), labeled using a Low Input Quick-Amp Labeling Kit (Agilent Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... direct RT-qPCRs were performed on heat-inactivated samples without extraction using the QuantiTect Reverse Transcription Kit (Qiagen). The reactions were run in an Applied Biosystems QuantStudio 7 Flex System (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: Total RNA was isolated from cells grown with and without IPTG using the RNeasy mini kit (Qiagen. Inc), followed by DNase treatment with the TURBO DNA-free kit (Invitrogen) ...
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bioRxiv - Plant Biology 2022Quote: Total RNA isolated from shoots and roots treated with or without bleomycin using an RNeasy Plant Mini Kit (Qiagen) was treated with DNase I (Qiagen ...
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bioRxiv - Genomics 2022Quote: ... or mock digestion (protocol without RNase R) followed by clean-up with the Qiagen RNeasy Mini kit (Q74106, Qiagen). RNA-seq libraries were prepared with the TruSeq Stranded Illumina Total RNA Preparation Kit (20020599 ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from tibial nerves (without epineurium) using QIAzol lysis reagent and was purified according to manufacturer’s instructions using RNeasy Kit (Qiagen). The concentration and purity of the RNA was determined with the ratio of absorption at 260/280 nm using NanoDrop 2000 Spectraphotometer ...
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bioRxiv - Immunology 2020Quote: ... miRNA inhibitors (miRCURY LNA miRNA power inhibitor with or without 5’-FAM, Qiagen) were added directly to culture media at the time of activation and were taken up by the cells by spontaneous translocation across the cell membrane ...
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bioRxiv - Genomics 2021Quote: ... 1XPIC) with or without 0.1l of 100mg/ml RNaseA (Qiagen cat. no. 19101) for 15 min on ice briefly tapped few times during incubation time and then centrifuged at 1000g for 5 min at 40C ...
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bioRxiv - Microbiology 2020Quote: ... the samples were diluted further with RTL buffer to give a 1:60 w/v homogenate and total nucleic acid was extracted from 300 μl of the clarified sample using the RNA tissue mini kit without DNase (Qiagen) and eluted in a 60 μl volume.
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from Doxycycline-inducible p53 expressing RPETert cells (treated with or without doxycycline) using the RNeasy kit (QIAGEN). 500 ng RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (BIO-RAD ...
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bioRxiv - Genetics 2021Quote: ... Swabs were stored without media at - 20°C until DNA was extracted with QiaAmp DNA Mini kit (Qiagen, Germantown, MD). Nasal shedding status of M ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA extracted from ID8 and OAW28 cells with or without SMARCA4-deficiency using the RNeasy Mini Kit (74104, Qiagen) was subjected to ribodeplete RNA-sequencing at MSK’s Integrated Genomics Operation (IGO) ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from five-day-old green seedlings with or without four hours of 27°C treatment of each genotype using the RNeasy Plant Kit (Qiagen). For mRNA sequencing (mRNA-seq ...
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bioRxiv - Physiology 2023Quote: Hypoxic cells were lysed in Buffer RLT without β-mercaptoethanol (Qiagen, Germantown, MD, USA). RNA extractions were carried out using an RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted for each of the persistently infected cells as well as the cell lines without NDV infection (negative control) using Qiagen RNeasy micro kit (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 65-79*SE or 65-79*PE (100 μg/mL) with or without IFN-γ (10 ng/mL) using the RNeasy mini kit (Qiagen) according to the vendor instructions ...
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bioRxiv - Molecular Biology 2023Quote: Cells were harvested after 72 hours siRNA knockdown with or without 4 hours etoposide treatment and genomic DNA was isolated with DNeasy Blood and Tissue kit (Qiagen # 69504). Nucleic acid was measured by nanodrop ...
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bioRxiv - Plant Biology 2023Quote: Frozen tissue samples were ground without a buffer suspension using a TissueLyzer II (Qiagen; 85300) that oscillated at 25Hz in 30 second increments ...
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bioRxiv - Microbiology 2019Quote: ... viral RNA was extracted from the egg-isolate of the virus from the nasal swab using the QIAmp viral RNA mini-kit without the addition of carrier RNA (Qiagen, Manchester, UK). cDNA was synthesized from RNA using a random hexamer primer mix and cDNA Synthesis System (Roche ...
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bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from the nasal swab using the QIAmp viral RNA mini kit without the addition of carrier RNA (Qiagen, Manchester, UK). cDNA was synthesised from RNA using a random hexamer primer mix and cDNA Synthesis System (Roche ...
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bioRxiv - Microbiology 2020Quote: ... Viral nucleic acids were then isolated using the Qiagen QIAamp MinElute Virus Spin Kit without the use of AW1 buffer or carrier RNA (Qiagen, Valencia, CA, USA). Random hexamers were used to prime cDNA synthesis (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... and wRB6) with or without shESRRG knockdown using a combination of TriZol and the RNeasy Mini RNA isolation kit (QIAGEN, Valencia, CA, USA). RNA-seq libraries were prepared using the TruSeq Stranded Total RNA prep kit with Ribo-Zero Gold to remove cytoplasmic and mitochondrial rRNA according to the manufacturer’s recommendation (Illumina ...
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bioRxiv - Genetics 2022Quote: ... the PCR fragment and the plasmid fragment without PDS gene were isolated from agarose gel using QIAquick Gel Extraction Kit (Qiagen, catalog number 28706). The digested PCR products and the pBSMVγ plasmid were ligated using T4 ligase (New England BioLabs ...
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bioRxiv - Cancer Biology 2023Quote: ... The resultant backbone without the Puromycin gene was purified using the QIAquick PCR & Gel Cleanup Kit (Qiagen 28506. A DNA block (Table 3) containing the Blasticidin resistance gene sequence was synthetised (Integrated DNA Technologies ...
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bioRxiv - Genomics 2023Quote: ... the microbial DNAs were extracted directly from the 20 Strain Staggered Mix Genomic Material (ATCC MSA-1003) without size selection using a QIAamp DNA stool mini kit (Qiagen, Valencia, CA, USA). For the human gut microbiomes ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from HPAEpiC with or without TGFβ1 or IL11 stimulation using RNeasy columns (Qiagen). RNA was quantified using Qubit™ RNA Broad Range Assay Kit (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing SctV9 without higher order aggregates of SctV were selected and incubated with 1.5 mL of Ni2+- NTA resin (Qiagen) pre-equilibrated in Buffer F (6 h ...
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bioRxiv - Cell Biology 2020Quote: ... and A10 encoding constructs or 1 μg Shh constructs with or without 0.5 μg Scube2 by using Polyfect (Qiagen). Cells were grown for 2 days or 3 days for Disp−/− rescue experiments at 37°C with 5% CO2 in DMEM containing 10% fetal calf serum and penicillin-streptomycin (100 μg/mL) ...
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bioRxiv - Genetics 2020Quote: ... and cells treated with transfection mix without siRNA (Wild type (WT)) or non-targeting control siRNA (scrambled (SCR)) (Qiagen catalog SI03650318 ...
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bioRxiv - Molecular Biology 2022Quote: ... and grown in serum-free medium for 24 hr and released into growth by addition of serum for 14 hr) by using RNA purification kit (QIAGEN). Purified RNAs were sequenced by GENEWIZ ...
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bioRxiv - Molecular Biology 2022Quote: ... serum or cell medium was extracted using the QIAmp DNA Mini Blood kit (Qiagen), used according to the ‘‘Blood and body fluid protocol’’ ...
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bioRxiv - Biochemistry 2021Quote: The plasmids pIB-spike-V5 with or without pIB-GALNTs-FLAG were transfected to S2R+ cells (DGRC) using Effectene transfection reagent (Qiagen) according to the instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The BmAgo3 immunoprecipitated beads were divided into two and incubated in buffer D with/without 200 μg/ml RNase A (Qiagen) at 30°C for 15 min ...
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bioRxiv - Genetics 2024Quote: ... and a negative control region within a regulatory region of the estrogen receptor substrate 1 (ESR1) without a predicted G4 motif (91) were PCR-amplified with HotStar Taq Polymerase (Qiagen). The PCR fragments were cleaned-up with Promega ReliaPrep DNA concentration kit and eluted in dH2O ...
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rpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencingbioRxiv - Microbiology 2019Quote: ... and were subjected to three cycles of grinding (1 minute, at 30 Hz followed by 1 minute without agitation) in a TissueLyser II apparatus (Qiagen, France). The solutions obtained from crushed nematodes or the contents of G ...
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rpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencingbioRxiv - Microbiology 2019Quote: ... IJs were crushed by three cycles of mechanical grinding (2 minutes at 30 Hz followed by 1 minute without agitation) in a TissueLyser II apparatus (Qiagen, France). We added 2 µL of Ready-Lyse Lysozyme Solution (Epi-centre ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in growth medium for one hour before lysis and RNA purification with the RNeasy Mini Plus Kit (Qiagen). Control cells were treated identically except for the five-minute addition of dissociating enzyme.
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bioRxiv - Microbiology 2019Quote: Strains were grown under agitating growth conditions in LB medium and total RNA isolation was performed using the RNeasy Mini kit (Qiagen). For removal of genomic DNA ...
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bioRxiv - Pathology 2022Quote: Total RNA was extracted from the lungs of uninfected and SARS-CoV-2 infected hamsters with or without immune suppression using Trizol reagent and purified by RNeasy mini columns (Qiagen, CA, USA) as described previously 61 ...