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Citations for Qiagen :
1 - 50 of 1570 citations for Mac 1 CD11b Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... CD11b+ cells were collected in RNA lysis buffer (Qiagen) and processed for real time quantitative PCR analysis.
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bioRxiv - Neuroscience 2023Quote: ... human THP-1 macrophages (MACs) and acutely isolated mouse microglia was extracted using the RNeasy Plus Mini kit (Qiagen, 74136) following manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... and probed with primary antibodies anti-5His monoclonal (1:1,000; QIAGEN), anti-Myc monoclonal (1:1,000 ...
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bioRxiv - Neuroscience 2024Quote: ... the enriched CD11b-positive cells were lysed using RLT buffer plus (Qiagen) supplemented with 40 μM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... RNA from MACS-purified cells was extracted using QIAshredder and RNeasy protocols (Qiagen). cDNA was amplified by Single Primer Isothermal Amplification (Ribo-SPIA® technology ...
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bioRxiv - Neuroscience 2020Quote: ... and isolated microglia/macrophage (CD11b+ cells) using RNeasy plus Mini kit (Qiagen, 74134). cDNA synthesis was performed using QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from CD11b-positive cells using the RNeasy Micro Kit (Qiagen). Libraries were prepared using Smart-Seq2 64 and modified for sequencing using the Nextera XT DNA Sample Preparation Kit (Illumina ...
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bioRxiv - Neuroscience 2021Quote: RNA was extracted from MACS sorted neurons (see above) using the RNeasy Micro Kit (Qiagen, 74004) following the manufactureŕs instructions ...
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bioRxiv - Neuroscience 2022Quote: ... CD11b+ CD45+ cells from one animal (2 retinas) were sorted directly into RLT buffer (QIAGEN 79216) and stored at -80°C ...
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bioRxiv - Immunology 2022Quote: ... MACS or FACS purified CD1c+ cells were immediately taken up in a lysis buffer (RLT plus, Qiagen) containing 1% β-mercaptoethanol ...
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bioRxiv - Genomics 2021Quote: ... One section was added to a MACs M tube (Miltenyi Biotech, U.K.) containing 1ml Qiazol (Qiagen, U.K.) and dissociated on a GentleMACs (Miltenyi Biotech ...
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bioRxiv - Immunology 2020Quote: RNA was isolated from MACS-sorted splenic B cells with the RNA Mini Kit (Qiagen, Hilden, Germany). Quantification of RNA was performed with a NanoPhotometer (Implen ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from CD11b+ cells and LK cells using RNeasy plus mini kit (Qiagen 74134) [source?] ...
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bioRxiv - Immunology 2019Quote: ... StrEP-Tag (mouse monoclonal, Qiagen, #34850). Peroxidase-conjugated secondary antibodies against rabbit IgG (#7074 ...
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bioRxiv - Biochemistry 2022Quote: ... A mouse monoclonal His-tetra (Qiagen) antibody (1:1000 dilution ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from FACS/MACS-isolated CD8+ T cells and reverse-transcribed using RNeasy Micro Kit (Qiagen) and SuperScript™ IV VILO™ Master Mix with ezDNase™ Enzyme (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated for 1 hour with monoclonal α-His antibodies conjugated to the horseradish peroxidase (1:5000 dilution; Qiagen). Blots were visualized with the addition of Lumina Forte Western HRP Substrate (MilliporeSigma ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... CD11b+ magnetic sorted microglia cells or embryonic neural progenitor cells were homogenized in QIAzol Lysis Reagent (#79306, QIAGEN) either immediately after isolation or after 10 days of in vitro culturing ...
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bioRxiv - Immunology 2022Quote: 5000-25000 P3/P4 LP macrophages from bone marrow chimeras 8 weeks following reconstitution were FACS-sorted (gated on: CD103-, Ly6G-, CD11b+, CD11cint, CD64+, Ly6C-, CD45.1+ or CD45.2+) directly into RLT buffer (Qiagen) on a FACS Aria II (BD Life Sciences ...
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bioRxiv - Immunology 2022Quote: Total RNA from ~ 5 × 105 neutrophils (CD45+Lin-CD11b+Ly6G+) was isolated with the RNeasy micro kit (Qiagen) and RNA quality was checked with the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Immunology 2023Quote: ... cytometry-sorted 7-AAD- CD45+CD11b+F4/80+ cells were lysed in 350µL RLT lysis buffer (Qiagen, 79216). At 4 days post injury ...
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bioRxiv - Immunology 2024Quote: ... as described previously.21 Lymphocytes were then purified using MACS LS Columns per the manufacturer’s instructions and sorted on the iCyt Synergy (Sony) into Buffer RLT (Qiagen). RNA was next extracted using the RNeasy Micro Kit (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... the mouse monoclonal Penta-His antibody (Qiagen n°34660 stock at 200 µg/ml ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from MACS-isolated whole-cortex microglia using the RNeasy Micro Kit per manufacturer instructions (Qiagen, Cat # 74004) and quantified on a NanoDrop 2000 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... a monoclonal mouse Strep-tag antibodies (#34850, Qiagen), a monoclonal anti-actin antibody (A5441 ...
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bioRxiv - Neuroscience 2022Quote: ... CD11b+) were sorted with the Beckman Coulter MoFlo XDP and were collected in 350 µl RNA lysis buffer (Qiagen, 1053393), and stored at −80°C until further use ...
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bioRxiv - Cancer Biology 2023Quote: ... epithelial cells (DAPI-EGFP+CD45-) and fibroblasts (DAPI-EGFP-CD45-CD11b-CD31-CD3-) were sorted and lysed in RLT buffer (Qiagen). Total RNA was prepared using RNeasy (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse brains with TRIzol or from selected CD11b or CD4-positive cells with RNeasy Plus Mini Kit (Qiagen) and reverse-transcribed ...
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... rat MPG were harvested and kept in RNAlater (Qiagen) immediately after cavernosometry ...
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bioRxiv - Cell Biology 2019Quote: ... then mixed with BioMag goat-rat IgG beads (Qiagen) and Ter119+ cells were depleted using a Dynal magnet (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... we also probed for the Strep-tag on γTuNA (1:1000 dilution of Strep-tag mouse monoclonal antibody, cat. # 34850, Qiagen). Band intensities were measured in ImageJ and normalized in Prism 7 by the wildtype γTuNA band (positive control) ...
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bioRxiv - Neuroscience 2021Quote: ... Lysis buffer was immediately added to CD11b+ cells for isolation of total RNA with the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany); alternatively ...
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bioRxiv - Synthetic Biology 2020Quote: ... For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen, #34660) and then incubated with 1:10000 peroxidase labelled anti-mouse IgG secondary antibody (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... followed by magnetic depletion using goat anti-rat beads (QIAGEN). For adoptive transfer experiments ...
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bioRxiv - Neuroscience 2019Quote: ... anti-Strep-tag II epitope mouse monoclonal antibody # 34850 (QIAGEN), anti-6xHis-tag mouse monoclonal antibody # H1029 (Sigma-Aldrich) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used Penta-His mouse monoclonal antibody (Qiagen, Qiagen #34660) at a 1:2000 dilution in 5% BSA in PBS-T buffer for 1 h ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We used Penta-His mouse monoclonal antibody (Qiagen, Qiagen #34660) at a 1:2000 dilution in 5% BSA in PBS-T buffer for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... blots were incubated with mouse monoclonal RGS-His antibody (Qiagen) diluted 1:1,000 ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were blocked with 5% milk in PBST (Phosphate buffer saline, 0.05% Tween 20) and incubated with monoclonal mouse anti-His antibody (Penta His, Qiagen, dilution 1:1000) according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cells were mixed with anti-rat Ig magnetic beads (Qiagen BioMag), incubated for 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... thymocytes were mixed with anti-rat IgG-conjugated BioMag beads (QIAgen) and incubated for 45 mins at 4°C on a MACSmix Tube Rotator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2019Quote: ... cells were incubated with anti-rat Ig magnetic beads (Qiagen BioMag), and subjected to magnetic separation ...
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bioRxiv - Cell Biology 2021Quote: ... was from List Biological Laboratories and the mouse monoclonal antibody against the His-tag from Qiagen.
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bioRxiv - Biochemistry 2023Quote: ... Bound PEX5L was detected immunologically using monoclonal Anti-His Antibodies (Qiagen).
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bioRxiv - Bioengineering 2022Quote: ... and native rat tissue samples were homogenized in lysis Buffer RLT (Qiagen). Total RNA was isolated using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with goat anti-rat beads (QIAGEN, cat. no. 310107). For intracellular staining ...
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bioRxiv - Biophysics 2020Quote: ... functionalized with neutravidin and treated with biotinylated anti-hexahistidine monoclonal antibody (Qiagen) as described (15) ...
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bioRxiv - Microbiology 2022Quote: ... and immunoblotted using a mouse monoclonal antibody against Strep-tag (Qiagen, 34850). Alkaline phosphatase conjugated to anti-mouse IgG (Sigma-Aldrich ...