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Citations for Qiagen :
1 - 50 of 512 citations for M CSF Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Approximately 2 × 106 undifferentiated MØP cells or M-CSF differentiated MØP cells were lysed in accordance with the Qiagen RNeasy Mini kit (Qiagen). The concentration of RNA was measured using either a Nanodrop™ 2000 (Thermofisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... miRCURY Exosome Cell/Urine/CSF Kit (Qiagen, 76743) was used to isolate EVs from conditioned medium according to the manufacturer instructions.
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bioRxiv - Developmental Biology 2023Quote: ... miRCURY Exosome Cell/Urine/CSF Kit (Qiagen, 76743) was used to isolate EVs from conditioned medium according to the manufacturer instructions ...
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bioRxiv - Biochemistry 2019Quote: ... and CHO-K1 cells respectively using a RNA isolation kit (RNeasy Mini Kit) from Qiagen. cDNAs were synthesized from the isolated RNA using a cDNA synthesis kit (Advantage RT-PCR kits ...
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bioRxiv - Microbiology 2023Quote: CSF was extracted by using the DNeasy Blood & Tissue Kit (Qiagen, 69504) with the following modifications ...
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bioRxiv - Neuroscience 2020Quote: Plcg2 knockdown was achieved in M-MØP cells using an antisense LNA GapmeR produced against mouse Plcg2 (Qiagen). Cells were grown to 80% confluence (∼2.4 × 105 cells per well ...
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bioRxiv - Physiology 2020Quote: ... RNA samples from heart tissue and HEK and CHO cell pellets were purified using a RNeasy mini kit (Qiagen). RNA was reverse-transcribed to cDNA using an Applied Biosystems High-Capacity RNA-to-cDNA kit (Foster City ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 M Tris (pH 7.0) (QIAGEN), 30% PEG 3000 (QIAGEN ...
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bioRxiv - Molecular Biology 2020Quote: ... The complex was initially crystallized in a buffer 0.1 M MES pH 6.5 and 0.6 M sodium fluoride from Anions Suite crystallization screen (Qiagen). After optimization of crystallization conditions ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... 2 μL of 0.1 M Dithiothreitol (Qiagen), 1 μL of 10 mM each dNTP ...
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bioRxiv - Neuroscience 2020Quote: Viral RNA was extracted from CSF and plasma using QIAamp® Viral RNA Mini Kit (Qiagen, CA). The viral RNA loads were determined by real time RT-PCR with the Taqman one-step RT-PCR master kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: CSF cell precipitates (approximately 5000 cells) was resuspended in 700 μl of QIAzol Lysis Reagent (Qiagen, Germany) and stored at −80°C ...
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bioRxiv - Immunology 2019Quote: RNA was extracted from blood and CSF using the PAXgene® Blood miRNA kit (Pre-Analytix, Qiagen, USA), quantified and RIN scores calculated using RNA Tapestation 4200® (Agilent ...
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bioRxiv - Immunology 2019Quote: ... CSF and whole blood for transcriptional profiling were collected in blood PAX-gene® (Pre-AnalytiX, Qiagen, USA) tubes ...
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bioRxiv - Microbiology 2020Quote: Load Viral RNA was isolated from plasma and cerebral spinal fluid (CSF) using the QuantiTech Virus Kit (Qiagen). Viral loads were quantified by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... Viral RNA from parietal cortex tissues and CSF was isolated by QIAamp Viral RNA Mini kit (Qiagen 1020953). qPCR of SIV gag RNA was by QuantiTect Virus kit (Qiagen 211011 ...
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bioRxiv - Biochemistry 2021Quote: ... 0.9 M KOAc [pH 4.8] (Qiagen N3 buffer). Centrifuge sample at 23°C for 10 minutes at 14,000 x g ...
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bioRxiv - Biochemistry 2023Quote: ... 0.9 M KOAc [pH 4.8] (Qiagen N3 buffer). Centrifuge sample at 23ºC for 10 minutes at 14,000 x g ...
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bioRxiv - Microbiology 2019Quote: ... Influenza virus nucleic load was determined by qRT-PCR using influenza virus segment M specific primers (IAV-M; Table. 1) in 96-well plates according to the manufacturer instructions (OneStep RT-PCR, Qiagen, Toronto, Canada). Absolute quantification was performed using a standard curve based on a 10-fold serial dilution of a plasmid containing the A/Guinea Fowl/129/2015(H5N9 ...
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bioRxiv - Biochemistry 2020Quote: ... 0.1 M Tris/HCl pH 8.5 (JCSG+ suite, Qiagen). Hanging drops of 1 ml protein at 2 mg/ml and 1 ml of precipitant (19% (w/v ...
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bioRxiv - Evolutionary Biology 2023Quote: ... HBV-DNA was extracted from 200 μL of serum/CSF samples using a QIAamp Blood Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Extracellular RNA was isolated from plasma and CSF using the QIAGEN miRNeasy Serum/Plasma Advanced Kit (Cat. No: 217204, QIAGEN) and following the vendor’s instructions with slight modifications ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 M Tris/Bicine pH 8.5 with 9 µL of JCSG+ (Qiagen) condition #29 containing 0.8 M sodium phosphate monobasic monohydrate ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was extracted from 140 µl of plasma and CSF samples using a QIAamp Viral RNA mini kit according to manufacturer’s instructions (QIAGEN Germantown, MD, USA; Cat# 52906). SIV gag RNA was quantified by qRT-PCR using the TaqMan RNA-to-Ct 1-Step Kit (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... 0.001 M of HCl at pH 3 was added and then homogenized (TissueRuptor, Qiagen) on ice ...
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bioRxiv - Plant Biology 2020Quote: ... and ground for one minute at 20 m/s in a TissueLyser II (QIAGEN). Then ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription for cDNA synthesis was performed using M-MuLV Reverse Transcriptase (Enzymatics, Qiagen) and RNAse Inhibitor (Biotechrabbit) ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from M-MDSCs using the miRNeasy Mini Kit (Qiagen, Germany). RNA libraries were prepared using TruSeq stranded mRNA sample prep kit (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 PPKS/M and 4 PPKS/Y) using the AllPrep DNA/RNA Mini Kit (Qiagen). Indexed libraries were generates using the KAPA mRNA HyperPrep kit and sequenced on a NextSeq500 sequencer (NextSeq 500/550 High Output Kit v2.5 ...
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bioRxiv - Bioengineering 2021Quote: RNA was extracted from ∼1 M cells using the QIAGEN RNeasy Mini Kit (QIAGEN 74104). A total of 36 samples were prepared ...
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bioRxiv - Molecular Biology 2021Quote: ... STAT3 (M-003544-02) or non-targeting AllStars negative control siRNA (1027280) were purchased from Qiagen or Dharmacon ...
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bioRxiv - Molecular Biology 2019Quote: ... with 0.2 M of IPTG at room temperature and was purified with Ni-NTA resin (Qiagen) with 50 mM sodium phosphate (pH 8.0) ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were incubated in 0.08 M KOH for 5 min and washed by Buffer EB (Qiagen). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.8□l of forward and reverse primer at 5□M concentration and 10□l QuantiNova (Qiagen), made up to a final volume of 20□l with nuclease-free water ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Systems Biology 2022Quote: ... 12 μl of 1 M Tris-HCl (pH 6.5) and 1 μl RNAse A (Qiagen cat # 19101) were added to the sample and incubated for 30 min at 37 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and homogenized twice at 10 s at 2 M/s with a 5-mm steal bead (Qiagen) using a tissue homogenizer (MP Biomedicals) ...
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bioRxiv - Genomics 2021Quote: ... One section was added to a MACs M tube (Miltenyi Biotech, U.K.) containing 1ml Qiazol (Qiagen, U.K.) and dissociated on a GentleMACs (Miltenyi Biotech ...
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bioRxiv - Microbiology 2023Quote: ... followed by rigorous shaking for 40s at 5.5 m/s in a Tissuelyser II (Qiagen, Hilden, Germany). Serial dilutions of these samples were then plated on agar-solidified culture media (Fig ...
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bioRxiv - Biochemistry 2022Quote: IQN17 was crystallized at room temperature in a hanging-drop vapor diffusion system by mixing 0.3 μL of 15 mg/mL peptide with 0.3 μL of reservoir solution containing 0.1 M imidazole (pH 8.0) and 35 % 2-methyl-2,4-pentanediol (MPD) (Qiagen). Crystals were seen two days after trays were set up ...
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bioRxiv - Plant Biology 2023Quote: ... with 0.2% (m/v) arabinose and the recombinant protein was affinity purified using Ni-NTA agarose (Qiagen). For GST-CPK3 ...
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bioRxiv - Physiology 2022Quote: ... Mouse Obesity (PAMM- 017ZC-12) array and Mouse Aging (PAMM-178ZC) from Qiagen (Maryland, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
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bioRxiv - Genomics 2019Quote: ... The partially decalcified nodule was then immersed in 1 mL of a digestion buffer with final concentrations of 0.45 M EDTA and 0.25 mg/mL Proteinase K (Qiagen) and rotated at 37°C overnight ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
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bioRxiv - Plant Biology 2020Quote: ... Magnetic beads (Dynabeads M-270 Carboxylic Acid) were activated and coated with ad hoc designed LNA probes (Qiagen), harboring an −NH2 group ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Cancer Biology 2020Quote: ... SAMHD1-targeting siRNA pool (siGENOME SMARTpool; M-013950-00-0005, Dharmacon) or control siRNA (AllStars Negative Control, Qiagen) were transfected at 10 nM final concentration.
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bioRxiv - Immunology 2020Quote: ... MuSK1A crystals were grown at 18 °C by vapor diffusion and after ≈ 4 days grew from drops consisting of 100 nL protein plus 100 nL of a reservoir solution consisting of 20% w/v PEG 6K (Precipitant) and 0.1 M BICINE 8.5 pH (Buffer) from the JCSG Core I (Qiagen) screen ...