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Citations for Qiagen :
1 - 50 of 75 citations for L Valine 13C5 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 99% Buffer TCL (Qiagen, cat# 1031576), sealed with perfluorinated oil (Sigma-Aldrich ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was extracted from the red blood cells of the parents and 99 F2 hybrid females using the DNeasy Blood & Tissue Kit (Qiagen). The library was constructed according to a previously described method [27] ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.8□l of forward and reverse primer at 5□M concentration and 10□l QuantiNova (Qiagen), made up to a final volume of 20□l with nuclease-free water ...
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bioRxiv - Systems Biology 2021Quote: ... and 200 L of Buffer AL (QIAGEN) were added to each sample before mixing with a pipette ...
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bioRxiv - Evolutionary Biology 2023Quote: ... trigynum (Sample size: L. tenue thrum=6, pin=4, L. trigynum homostyle=5) using the RNeasy Plant Mini Kit (QIAGEN, Germany). Libraries were sequenced on an Illumina NovaSeq S1 Sequencing System to produce paired-end 150bp read length reads ...
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bioRxiv - Immunology 2022Quote: ... T6BP (L-016892-00-0020 Dharmacon or SI02781296, Qiagen), CANX (SI02663367 and SI02757300 ...
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bioRxiv - Systems Biology 2022Quote: ... FABP (horizon L-042923-01-0005) and HIF1α (Qiagen SI00193032 ...
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bioRxiv - Microbiology 2020Quote: ... 0.01% L-tryptophan) using the RNeasy Mini kit (Qiagen). Ten μg of total RNA was hybridized to 150 nM 32P 5’-end-labeled DNA oligonucleotide complementary to nucleotides +94 to +109 (relative to yfmHG transcription ...
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bioRxiv - Genetics 2022Quote: ... Quantified libraries (GeneRead DNA Library L Core Kit; QIAGEN) were sequenced using an Ion Torrent S5 XL instrument with Ion 530 chips and respective reagents (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The samples were homogenized in 180 l of ATL Buffer (Qiagen) with ~20 1-mm glass beads (BioSpec ...
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bioRxiv - Microbiology 2019Quote: ... 0.125 μl Taq DNA Polymerase (5 units/L, Qiagen, Hilden, Germany) and 1 μl of DNA template ...
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bioRxiv - Genetics 2020Quote: ... and adapter ligating (T4 DNA Ligase #L603-HC-L, Enzymatics/Qiagen). Final cleanup and size selection was performed using 1-part DNA ...
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bioRxiv - Biochemistry 2021Quote: ... then supplemented with 15 mL/L settled Ni-NTA resin (Qiagen) at a scale of 12 L ...
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bioRxiv - Cancer Biology 2019Quote: ... 12 μL of 20 μmol/L negative control siRNA (QIAGEN, Germantown, MD) or Hs_MAN1B1 siRNA (QIAGEN ...
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bioRxiv - Biophysics 2022Quote: ... combined with Ni-NTA resin (2 mL/1 L of biomass, Qiagen) pre-equilibrated with 40 mM sodium phosphate buffer ...
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bioRxiv - Biophysics 2022Quote: ... supernatant was combined with Ni-NTA resin (1 mL/1 L of biomass, Qiagen) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Pathology 2021Quote: ... Ion Torrent-specific sequencing libraries were generated using the GeneRead L Core Kit (Qiagen, Hilden, Germany) together with IonXpress barcode adaptors (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: HUVECs were transfected at 60% confluency with 10 nmol/L locked nucleic acid (LNA) GapmeRs (Qiagen) or small interfering RNAs (siRNAs ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from aged and aged Nox4-/+ L-MSCs using RNeasy® Mini Kit (Qiagen) and reverse transcribed using iScript Reverse Transcription SuperMix for RT-qPCR (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... The enriched and purified samples were amplified using the GeneRead DNA Library L amplification Kit (Qiagen, Germany) according to manufacturer’s instructions with 14 cycles and amplicons were purified using solid-phase paramagnetic bead technology ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was supplemented with Ni-NTA agarose (Qiagen, 1.3 ml resin/ 1 l insect cell pellet). Subsequently ...
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bioRxiv - Genomics 2023Quote: ... 2μL DNA was amplified in a 30μL reaction consisting of 14.33μL QuantiTect Multiplex PCR NoROX mix (Qiagen), 0.67μL QuantiTect Multiplex PCR mix with ROX (Qiagen) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1.0–3.0 μl (5.0–15 pmol) of primers and 7.5–15 μl of 2x Multiplex PCR Plus Master mix (QIAGEN). The PCR protocol consisted of an initial DNA polymerase (HotStar Taq ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet, Qiagen) and incubated for 30 mins at 4 °C on a rotating wheel ...
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bioRxiv - Microbiology 2019Quote: ... The final concentrated solution was transferred to a microtube containing Pathogen lysis tube L beads (Qiagen, Hilden, Germany), 50 μl of Solution SL and 200 μl of sterile saline solution to homogenize ...
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bioRxiv - Neuroscience 2019Quote: ... The cDNA was measured in triplicates by RT-qPCR (reaction volume 15 l) using Rotor-Gene Q (Qiagen) and Brilliant III Ultra-Fast SYBR Green qPCR Master Mix (Agilent) ...
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bioRxiv - Cancer Biology 2023Quote: ... and Huh6 cell lines with siRNAs targeting PTPRK or a negative control siRNA (working concentration 30nmol/L; QIAGEN). The delivery of siRNA was achieved using Lipofectamine™ RNAiMAX Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... An aliquot (16 μl) of the enriched samples was amplified using the GeneRead DNA Library L amplification Kit (Qiagen) according to manufacturer’s instructions performing 14 cycles and purified using solid-phase paramagnetic bead technology ...
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bioRxiv - Neuroscience 2019Quote: ... Pools of 150 RFP+ and RFP− individual cells were laser captured from the granular cell layer of the dentate gyrus of each mouse using a Leica Microsystem LMD system under a HC PL FL L 63/0.60 XT objective and collected in lysis buffer (50 ul RLT buffer, Qiagen). Total RNA from LCM samples was isolated separately from each animal.
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bioRxiv - Microbiology 2019Quote: ... Samples were neutralized with 0.7 µL of a 7.5 M HCl stock and the remaining cDNA was purified using a nucleotide removal kit (Qiagen). Sequencing reactions were performed according to the method of Sanger ...
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bioRxiv - Cell Biology 2020Quote: ... 35 nL cell lysis buffer was added to each microwell (P1: 2.89 AU/L Protease K (19155, Qiagen, Germany) and 72.8 mM pH 7.5 Tris•HCl (15567027 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10µL of diluted nuclei were distributed to all wells of a 96-well plate and 1µL EB buffer (Qiagen) 0.5µL Proteinase K (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified bacterial lysates from a 1 l culture were bound to a 0.5 ml column of Ni-NTA resin (Qiagen) by gravity flow ...
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bioRxiv - Genetics 2023Quote: ... leaf tissues were collected and homogenized in 400 μL of TPS buffer (100 mM Tris-HCl, 10 mM EDTA, 1 M KCl, pH8.0) using TissueLyser II (Qiagen), followed by incubation for 20 min at 75°C ...
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bioRxiv - Immunology 2022Quote: ... 2 μL of cDNA was added to 19 μL first round PCR reaction mix containing 0.5 U HotStarTaq Polymerase (Qiagen), 0.18 μM of each 3’ reverse primer ...
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bioRxiv - Genomics 2023Quote: ... Each 1 μl uracil cleavage mix contained 0.5 μl Uracil DNA Glycosylase (Enzymatics G5010) and 0.5 μl Elution Buffer (QIAGEN 19086). Unincorporated DNA oligos were digested at 37 °C for 30 minutes using a thermocycler ...
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bioRxiv - Genomics 2020Quote: ... The 96-well plates contained 12μl per well of reverse cross-linking buffer (0.83 mg/ml Proteinase K and 0.042% SDS in Qiagen EB buffer), and were put on ice ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from 18 cultures that were growing in LB+colistin (2mg/L) using DNeasy Blood and Tissue Kit (QIAGEN)
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bioRxiv - Genomics 2022Quote: ... 100 µl of lysis buffer was added to each 5mm punch biopsy and subjected to lysis using the TissueLyser II (Qiagen), at speed of 30 Hz for 90 seconds ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl clarified lysate were mixed with 70 μl RNase free water and RNA isolated using the RNeasy Mini Kit (Qiagen) according the manufacturers recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... Biotin-labelled RNA was eluted with 100 μl elution buffer (100 mM DTT in nuclease-free water) and cleaned up with the RNeasy MinElute Cleanup kit (QIAGEN), adjusting the amount of ethanol to capture RNA < 200 nucleotides in length ...
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bioRxiv - Microbiology 2022Quote: For RNAseq the pellets were resuspended in 150 µL 10 mM Tris-HCl pH 8 and mixed with 700 µL of ice cold RLT+BME (RLT buffer (Qiagen) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Microbiology 2020Quote: ... The eluant was treated with DNase I (1 U/μl) to digest non-specific DNA and the bound DNA was purified from the complex via PCR Qiagen Kit (Qiagen). The purified DNA was amplified with primer sets specific to the prrF1,F2 promoter (Table S2 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The samples were purified using 500 μl PB and 650 μl of PE buffer and eluted in 30 μl EB buffer (MinElute PCR Purification Kit, QIAGEN). The adaptor ligation module was implemented using 10 μl of buffer ...
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bioRxiv - Immunology 2021Quote: THP-1 human monocytes or mature primary human peripheral blood derived macrophages were transfected with control (D-001810-10-05, Dharmacon) or SP140 (L-016508-00-0005, Dharmacon) SMARTpool siRNA (100nM) using HiPerfect reagent (Qiagen) or RNAiMAX (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... were annealed (10 μL each 100 μM) to 10 μL 5′-[Phos]-CTGTCTCTTATACACATCT-3’ oligonucleotide (100 μM) within 80 μL EB buffer (Qiagen), incubating 2 min at 95°C and cooled to room temperature (0.1°C/sec) ...
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bioRxiv - Genomics 2022Quote: ... 32 μl Master Mix containing 30 μl MDA reaction buffer and 2 μl Phi29 polymerase (REPLI-g UltraFast Mini Kit; Qiagen) were added ...
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bioRxiv - Microbiology 2022Quote: ... and 150 μL DMEM supplemented with 2% FBS and homogenized using a TissueLyser II (2 cycles at 30 Hz, 1 min, Qiagen). Homogenates were clarified by centrifugation and frozen until plaque assay titration ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from FACS-sorted L-GMPs from BL/6 and Il21-/- AML mice using the RNeasy Micro Kit (Qiagen). RNA purity was checked using the NanoPhotometer® spectrophotometer (Implen) ...
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bioRxiv - Microbiology 2023Quote: ... fresh 0.35 g/L proteinase K) during two rounds of 3 minutes at a 30Hz/s frequency in a TissueLyser II grinder (Qiagen). Total RNA was converted into complementary DNA (cDNA ...