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Citations for Qiagen :
1 - 50 of 1117 citations for Kanamycin kinase type II NEO Klebsiella pneumoniae since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... pneumoniae ST846-OXA48 culture (Minutesiprep, Qiagen, Hilden, Germany). The khe and blaOXA-48 genes were consequently amplified from 5 µL of the extract by PrimeSTAR HS DNA polymerase ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was prepared from pure culture of Klebsiella isolates using DNeasy Blood and Tissue kit (Qiagen) and whole genomes were sequenced on an Illumina HiSeq 2000 platform ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA for each Klebsiella strain was extracted using the AllPrep Bacterial DNA/RNA/Protein kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... pneumoniae D39 WT and all FQ-resistant mutants using the DNeasy Blood & Tissue Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s recommendations for Gram-positive bacteria ...
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In-silico enrichment of bacterial plasmids by chromosomal depletion using nanopore adaptive samplingbioRxiv - Genomics 2022Quote: ... Klebsiella pneumoniae (GCF_025837075.1) and Enterobacter hormaechei (GCF_001729785.1) DNA was extracted using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was homogenised to a fine powder using a ball mill-type grinder (Tissuelyser II Qiagen, 85300). For the homogenization of the frozen material one liquid nitrogen cooled 5 mm stainless steel metal balls was added to each Eppendorf tube and the frozen material was disintegrated for 1 min at 25 Hz.
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bioRxiv - Systems Biology 2023Quote: ... and 20 µL Protein Kinase K (Qiagen) for 30 min at RT followed by the steps as described in the protocol of Dneasy Blood & Tissue Kit (QIAGEN) ...
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bioRxiv - Molecular Biology 2023Quote: ... Polynucleotide Kinase and Klenow DNA Polymerase (Qiagen). An A base was added to the 3’ ends with Klenow (3’-4’ exo- ...
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bioRxiv - Microbiology 2021Quote: ... and the kanamycin resistant phenotype using the RNeasy PowerSoil Total RNA Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Lentivirus carrying the response elements for type I (ISRE - #CLS-008L-1) or type II (GAS - #CLS-009L-1) upstream of firefly luciferase was purchased from Qiagen. Twenty-four hours post plating ...
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bioRxiv - Systems Biology 2022Quote: ... We knocked down kinases using siRNAs from Qiagen (see spreadsheet for the ordering numbers of the siRNAs used ...
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bioRxiv - Genomics 2023Quote: ... californicus (neo-W chromosome race) was extracted separately using the MagAttract HMW DNA kit (Qiagen) and labeled using the SureTag Complete DNA Labeling Kit (Agilent ...
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bioRxiv - Cancer Biology 2019Quote: ... using specific primers for each silenced kinase (QuantiTect, #249900, Qiagen).
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bioRxiv - Molecular Biology 2023Quote: A siRNA library targeting all human kinases (Qiagen, Hilden, Germany) was utilized ...
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bioRxiv - Microbiology 2020Quote: ... Clones growing only in Kanamycin medium were expanded and concatemers extracted and purified using MAXI prep (Qiagen). Recovered DNA was tested by PCR to confirm the LTR junction ...
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bioRxiv - Genomics 2022Quote: ... 300 μl of Protein Kinase Digestion (PKD) buffer (Qiagen, Cat. No. 169021771) was added to sample tubes ...
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bioRxiv - Microbiology 2023Quote: ... The two fragments of yfhO and the fragment with the kanamycin resistance cassette were purified using the QIAquick PCR purification kit (Qiagen) and fused by Gibson Assembly (SGA-DNA ...
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bioRxiv - Neuroscience 2024Quote: ... Overnight cultures of the positive clones were grown in LB Kanamycin (30 μg/mL) media and plasmid DNA was extracted using the QIAprep Spin Miniprep Kit (Qiagen). Purified plasmid DNA was sequenced using ABI BigDye Terminator v3.1 Sequencing at the Australian with the following primers ...
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bioRxiv - Microbiology 2019Quote: ... TissueLyser II (Qiagen) was further used to break heterocysts at frequency of 30/s for 8 min ...
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bioRxiv - Genetics 2021Quote: ... 0.4 μl H2O (Type-it Kit, QIAGEN) and 75 μg of template DNA ...
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bioRxiv - Genetics 2021Quote: ... 0.2 μl Q (Type-it Kit, QIAGEN), 0.4 μl H2O (Type-it Kit ...
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bioRxiv - Developmental Biology 2020Quote: ... EvaGreen (Type-it HRM PCR Kit, Qiagen) and the manufacturers recommended protocol for use ...
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bioRxiv - Molecular Biology 2023Quote: ... EvaGreen (Type-it HRM PCR Kit, Qiagen) and the manufacturers recommended protocol for use ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 1 μl Type-it Master Mix (Qiagen), 0.17 μM of either FAM or VIC ...
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bioRxiv - Plant Biology 2019Quote: ... Bacteria were grown overnight at 37°C on LB plates with 50 μg/mL kanamycin and plasmids from randomly selected positive clones were purified using QIAprep Spin Miniprep kit (QIAGEN, Germany) and Sanger sequenced (Genoscreen ...
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bioRxiv - Microbiology 2023Quote: ... Single colonies were picked and grown overnight in LB broth with 50 μg/mL Kanamycin before plasmids were isolated using a QIAprep Spin Mini Kit (Qiagen, Germantown, MD), confirmed by restriction enzyme digest ...
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bioRxiv - Genetics 2019Quote: ... 1× Type-it Multiplex PCR Master Mix (Qiagen), 0.2 μM of each locus specific reverse primers ...
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bioRxiv - Microbiology 2019Quote: ... The TissueLyser II (QIAGEN) was set to 30 hz/sec for a total of 15 mins for the pools of whole mosquito samples ...
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bioRxiv - Molecular Biology 2020Quote: ... using TissueLyser II (Qiagen). Quantification of total protein concentration was assessed by the bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2021Quote: ... and Tissuelyser II (Qiagen). Total RNA were extracted from roots 5 days after germination using the RNeasy® Plant Mini kit (Qiagen #74904 ...
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bioRxiv - Cell Biology 2021Quote: ... using TissueLyzer II (Qiagen) for 30 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... using TissueLyser II (Qiagen) at 25Hz for 2mins ...
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bioRxiv - Synthetic Biology 2021Quote: ... using TissueLyser II (Qiagen) at 25Hz for 2mins ...
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bioRxiv - Cancer Biology 2023Quote: ... using TissueLyser II (Qiagen) homogenizer at 4°C following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... using Tissuelyser II (Qiagen) and steel beads of 5 mm diameter ...
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bioRxiv - Microbiology 2019Quote: ... The stool samples were homogenized at 1,500 rpm for 20 min with Shake Master Neo (Biomedical Sciences, Tokyo, Japan) after adding the Inhibit EX buffer from the QIAamp Fast DNA Stool Mini Kit (Qiagen, Hilden, Germany). Genomic DNA was then extracted using the kit according to the manufacturer’s instructions and was re-suspended in 10 mM Tris-HCl buffer at 5 ng/μL ...
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bioRxiv - Biophysics 2023Quote: ... QIAEX II Gel Extraction Kit was used (50 µL of QIAEX II beads, QIAGEN). The samples were washed twice with PE buffer and eluted twice by 125 µL of 10 mM Tris-HCI buffer ...
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bioRxiv - Cell Biology 2020Quote: ... using a TissueLyser II (Qiagen). Samples were clarified by centrifugation at 17000 x g for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... in a TissueLyser II (Qiagen) at 20s−1 for 3min ...
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bioRxiv - Microbiology 2019Quote: ... using the TissueLyser II (QIAGEN) with two sets of 96-well adapter plates (QIAGEN ...
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bioRxiv - Neuroscience 2020Quote: ... using TissueRuptor II (QIAGEN, 9002756), then lysed for 1 hour on ice ...
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bioRxiv - Neuroscience 2021Quote: ... using a TissueRuptor II (Qiagen). Chloroform was added and RNA subsequently extracted following the spin-column protocol from the miRNeasy kit with DNase digestion (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... and the TissueLyser II (Qiagen). For plaque assays ...
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bioRxiv - Physiology 2021Quote: ... using a TissueLyser II (Qiagen), followed by sonication and centrifugation (10 min at 12,000 rpm at 4°C) ...
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bioRxiv - Microbiology 2022Quote: ... and a TissueLyser II (Qiagen) (carcasses) ...
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bioRxiv - Cell Biology 2021Quote: ... using the TissueLyser II (Qiagen) for 30 minutes ...
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bioRxiv - Immunology 2019Quote: ... using a TissueLyser II (Qiagen), centrifuged for 3 min at 12,000 × g and the lysate was transferred to a clean tube.
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bioRxiv - Pathology 2020Quote: ... with a TissueLyser II (Qiagen).
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bioRxiv - Genetics 2021Quote: ... Following homogenization (Qiagen TissueLyser II), sonication and centrifugation (20,000 x g ...
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bioRxiv - Cell Biology 2021Quote: ... with Tissue Lyzer II (Qiagen) and processed with RNeasy kit (Qiagen ...