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Citations for Qiagen :
1 - 50 of 107 citations for Ion Exchange Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The Klenow polymerization reactions were incubated at 37 °C for 1 h and then purified on ion-exchange cartridges (cat. no. 28306; Qiagen). The 194 purified reactions were dissolved in 50 µL of water each and pooled to generate the 3,880 members library (DEL3880 ...
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bioRxiv - Cell Biology 2022Quote: ... anion exchange chromatography and by Ni-NTA-Agarose (Qiagen #1018244) affinity chromatography as described previously [2,39–41] ...
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bioRxiv - Physiology 2022Quote: ... Using the Neuronal ion channel plate (Qiagen, UK), 84 ion channels as well as housekeepers were measured in each sample ...
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bioRxiv - Biophysics 2021Quote: ... DNA plasmids were purified using anion-exchange gravity flow chromatography (Qiagen, Hilden, Germany).
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was collected and purified using Nickel ion affinity chromatography (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Ion-chelated IMAC beads were prepared from Ni-NTA Superflow agarose beads (Qiagen, MA). Nickel ion was stripped with 50 mM EDTA and iron was chelated by passing the beads through aqueous solution of 200 mM FeCl3 followed by three times of water wash and one time wash with binding buffer (40% acetonitrile ...
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bioRxiv - Pathology 2021Quote: ... Ion Torrent-specific sequencing libraries were generated using the GeneRead L Core Kit (Qiagen, Hilden, Germany) together with IonXpress barcode adaptors (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... which was performed by batch immobilized metal ion affinity (IMAC) using nickel agarose slurry (Ni-NTA, QIAGEN or Ni Sepharose High Performance ...
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bioRxiv - Microbiology 2022Quote: ... 6xHis tagged protein were purified by immobilized metal ion chromatography using nickel-NTA super flow resin (Qiagen) as previously described with slight modifications46 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were further purified by reverse immobilized metal ion affinity chromatography (IMAC) using TALON resin (Qiagen), to remove uncleaved protein ...
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bioRxiv - Developmental Biology 2023Quote: ... BeWo conditioned media and MEHP (10μM) + BeWo conditioned media) using the RNeasy PLUS Mini Kit from Qiagen (Germantown, MD). RNA extraction followed the manufacturer instructions ...
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bioRxiv - Physiology 2021Quote: ... the supernatant was harvested by centrifugation for 20 min at 10,000 g and then loaded onto 5 ml Immobilized Metal-ion Affinity chromatography (IMAC, Ni-NTA Agarose, Qiagen) pre-equilibrated in 20 mM Hepes pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... for the isogenic pair (APOE3 and APOE4) of iMGLs exposed to spheroid conditioned media or unspent neuronal media was achieved with RNeasy Plus Mini Kit (Qiagen). RNA integrity number (RIN ...
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bioRxiv - Immunology 2023Quote: ... the media was aspirated and RLT buffer (Qiagen) was added to the wells ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and dARC1 CA captured from clarified lysate using immobilised metal ion affinity on a 5 mL Ni2+-NTA superflow column (Qiagen). Bound dARC1 CA was eluted in non-reducing buffer (50 mM Tris-HCl ...
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bioRxiv - Genetics 2020Quote: Iron nitrilotriacetic acid (NTA) resin were prepared in-house by stripping metal ions from nickel nitrilotriacetic acid agarose resin (Qiagen) with 100 mM ethylenediaminetetraacetic (EDTA ...
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bioRxiv - Microbiology 2022Quote: ... The Sanger sequencing data and Ion Torrent BAM files were analyzed using CLC Genomics Workbench (Qiagen, CLC bio, Aarhus, Denmark), including running BLAST against the NCBI database (downloaded in May 2021).
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bioRxiv - Molecular Biology 2023Quote: ... The nucleosides were quantified using the nucleoside to base ion mass transitions and analyzed as the ratio of m6A was applied to a RNeasy Mini Kit (Qiagen) and RNA was / A or m6A / A ...
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bioRxiv - Microbiology 2021Quote: ... 40 μl of media from each of 6 plant wells or media from 3 minimal media wells were pooled and stabilized in RNAprotect® Bacteria Reagent (QIAGEN) before performing RNA extraction using RNeasy Mini Kit (QIAGEN) ...
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bioRxiv - Bioengineering 2020Quote: ... the resulting supernatant containing soluble LCC was subjected to purification by immobilized metal ion chromatography (IMAC) using Ni-NTA Superflow (Qiagen, Hilden, Germany). Protein elution using 250 mM imidazole was then dialyzed against 100 mM potassium phosphate buffer (pH 8 ...
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bioRxiv - Cell Biology 2020Quote: ... media containing cadherin was flowed through Ni-NTA agarose beads (Qiagen) packed on 1 ml Empty Bio-Scale Mini Cartridges (Biorad #7324660) ...
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bioRxiv - Biochemistry 2020Quote: ... Media was removed by filtration using a QIAvac 96 vacuum manifold (Qiagen) and wells were washed two times with 150 μL media ...
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bioRxiv - Genomics 2022Quote: ... exRNA was isolated from cell culture media by two methods: exoRNeasy (Qiagen) and Exosome Total Isolation Chip (ExoTIC) ...
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bioRxiv - Developmental Biology 2024Quote: ... media was removed and cells were lysed with Qiazol (Qiagen, Cat. #79306). RNA extraction was performed according to manufacturer’s guidelines ...
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bioRxiv - Microbiology 2019Quote: RNA was isolated from growth media using QIAmp Viral RNA Mini Kit (Qiagen). Reverse transcription was performed using ProtoScript First Strand cDNA Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... media were aspirated and cells were lysed with RLT plus lysis buffer (Qiagen). Lysates were transferred to 1.5 ml tubes and were fully homogenized using a handheld pestle and mortar ...
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bioRxiv - Microbiology 2020Quote: ... virus media was removed and cells were harvested using RNAprotect Cell Reagent (Qiagen). RNA was extracted using RNeasy columns (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... The media was then incubated with 1.5 mL of Ni-NTA beads (Qiagen) at 4°C on a rolling bed for 1 hr ...
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bioRxiv - Bioengineering 2024Quote: ... Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen) and eluted with DPBS containing 150 mM imidazole.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were recovered into growth media and then resuspended in RLT lysis buffer (Qiagen). RNA extraction was performed using the Qiagen RNeasy kit according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the media was collected and applied to 3 ml of Ni-NTA resin (Qiagen). The resin was collected and washed with PBS supplemented with 20 mM imidazole ...
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bioRxiv - Developmental Biology 2021Quote: ... the media was taken off and cells were lysed directly in Qiazol (Qiagen, 79306), followed by RNA preparation.
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bioRxiv - Molecular Biology 2022Quote: ... change fresh media gently and transfect cells according to Effectene Transfection Reagent Kit (QIAGEN).
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bioRxiv - Bioengineering 2023Quote: RNA was extracted from media samples using QIAamp Viral RNA Mini Kit (250) (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and aliquots stored in viral transport media (VTM) and AVL buffer (Qiagen, Milton Keynes, UK) at −80°C until assay.
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bioRxiv - Microbiology 2021Quote: ... Total RNA extraction of the media was performed using Qiamp viral RNA Mini Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Culture media or cells were collected for RNA extraction using an RNA extraction kit (Qiagen). DENV-2 copy number was quantified by one step real time RT-PCR as previously described (16) ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from 140 µl of cultured media using QIAamp RNA extraction kit (Qiagen) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: DNA extraction from media was performed using the DNeasy Blood and Tissue kit (QIAGEN, Hilden, Germany) following manufacturer’s protocols ...
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bioRxiv - Bioengineering 2022Quote: RNA was extracted from supernatant culture media using the QIAamp 96 Virus QIAcube HT Kit (Qiagen). E-gene expression was determined using the SensiFAST Probe No-Rox One Step Kit (Bioline ...
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bioRxiv - Cell Biology 2021Quote: ... Media and osteoblasts were harvested 3 days later and EVs were collected using Exoeasy kit (Qiagen). Total RNA was extracted using miRNeasy micro (Qiagen).
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bioRxiv - Biophysics 2021Quote: ... Media containing his-tagged Ecads was passed through a chromatography column containing Ni-NTA agarose beads (Qiagen). Beads were then washed with a pH 7.5 biotinylation buffer (25mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... Nucleic acid was extracted from cell culture media manually using a QIAamp Viral RNA Mini Kit (QIAGEN) or using NucliSENS easyMAG or EMAG platforms (both BioMérieux) ...
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bioRxiv - Biophysics 2022Quote: ... Media containing his-tagged Ecads was passed through a chromatography column containing Ni-NTA agarose beads (Qiagen). Beads were then washed with a pH 7.5 biotinylation buffer (25 mM HEPES ...
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bioRxiv - Genomics 2019Quote: ... Cell-free DNA was extracted from the thawed media using the Qiagen circulating DNA extraction kit (Qiagen) according to the manufacturer’s instructions and quantified using Qubit High Sensitivity DNA quantification kit (Life Technologies) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The RBD was purified from the expression media by metal-affinity chromatography using Ni-NTA beads (Qiagen). The Fabs were purified from the expression media using rProtein A Sepharose Fast Flow beads (GE healthcare) ...
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bioRxiv - Genomics 2021Quote: ... cells were lysed by removing media from culture dishes and adding 200ul of RLT plus buffer (Qiagen) supplemented with 0.5mM 2-mercaptoethanol ...
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bioRxiv - Immunology 2024Quote: ... in serum-free media containing a transfection reagent (HiPerFect Transfection Reagent, catalog no. 301704; Qiagen, Valencia, CA). M- or G-MDSC cultures were subjected to a transient elastase treatment (0.4 U ...
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bioRxiv - Cell Biology 2022Quote: ... total RNA was extracted from EV enriched samples isolated from conditioned media using the miRNeasy Micro kit (QIAGEN), again following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... The concentrated SPACA6-containing media was applied onto a 2-mL column of Ni-NTA agarose resin (Qiagen). The Ni-NTA resin was washed with 10 column volumes (CV ...