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Citations for Qiagen :
1 - 50 of 10000+ citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from in vitro activated CD4+ T cells using RNeasy Mini Kit (Qiagen Inc.) as described in manufacturer’s protocol including an on-column digestion step (RNaseFree DNase Set ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from 0.5×106 naïve and activated CD8+ T cells using a using an RNeasy Mini kit (Qiagen) and additionally purified using an RNase Free DNase Set (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... total RNA of CD8+ human T cells was extracted by miRNeasy Mini Kit (Qiagen), following the manufacturers’ instructions ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA from human primary T cells was isolated using Gentra Puregene Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: Genomic DNA from human primary CD4+/CD8+ T cells was isolated using the Gentra Puregene Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... high molecular weight genomic DNA (gDNA) was purified from human primary T cells using the Blood & Cell Culture DNA Maxi Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA from 1-5 million total CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen) or phenol-chloroform and the DNA concentration was measured by Qubit High Sensitivity Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from 5 x 106 T cells using the RNeasy® Mini Kit (Qiagen, Cat. 74106). RNA extraction was performed following the instructions from the “Purification of Total RNA from Animal Cells Using Spin Technology” protocol given in the RNeasy® Mini Handbook ...
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bioRxiv - Immunology 2023Quote: RNA was purified from at least 5 x 104 CD4+ T cells using the RNeasy Micro kit (Qiagen). cDNA was synthesized using the SuperScriptTMVILOTMcDNA synthesis kit (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted from quiescent and activated cells using an RNeasy kit (Qiagen) for RNA-seq.
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from 5 million transfected Jurkat T-cells using the DNeasy Blood and Tissue kit (Qiagen) and quantified by Quant-iT™ PicoGreen™ dsDNA Assay kit (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: Cultured human T cells after treatment were flash frozen in Buffer RLT (Qiagen, 79216) and kept in −80°C until processing ...
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bioRxiv - Immunology 2020Quote: ... Within these gates CD8+MR1-5-OP-RU- and CD8+MR1-5-OP-RU+ T cell populations were sorted directly into lysis buffer for RNA isolation (RNeasy Plus Micro kit, Qiagen). Purity of ungated sorted CD8+humMR1-5-OP-RU+ cells was between 91-99% (Fig ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from cultured in vitro or in vivo primed T cells or ex vivo sorted T cells using the RNeasy kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted from purified human T cells using the RNeasy Plus Minikit (Qiagen, Germantown, MD). 0.5 μg Donor B RNA was mixed with 4.5 μg Donor A RNA to create sample C for quantitation experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... the nuclear pellet was sonicated for 5 min and genomic DNA was extracted using the QIAamp DNA Mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... the nuclear pellet was sonicated for 5 min and nuclear gDNA was extracted following the protocol of the DNeasy Blood & Tissue kit (Qiagen) with addition of RNase A treatment.
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bioRxiv - Immunology 2020Quote: ... or CD4+ T cells was extracted using the RNeasy micro kit (Qiagen) followed by cDNA synthesis using the iScript cDNA synthesis kit (BioRad) ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from T cells using the RNeasy kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from isolated CD8+ T cells (RNeasy kit, Qiagen) and 300 ng of RNA were used for cDNA synthesis (First-Strand Synthesis kit ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from murine T cells via RNeasy Kit (Qiagen) with DNase digestion (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... and CD95+ CD4 T cells using a RNeasy plus mini kit (QIAgen). Isolated RNA sample quality was assessed using a BioAnalyzer RNA pico assay (Agilent Technologies Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... shRNA expressing cells were isolated by fluorescence activated cell sorting at passage doubling 1 and 15 followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Sequencing libraries were prepared by subjecting 18 μg (1.2 μg x 15 reactions ...
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bioRxiv - Immunology 2020Quote: RNA from isolated fresh B cells and B cells activated with LPS/IL-4 was extracted using a RNeasy kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... total mRNA was isolated from mouse T cells by RNeasy Micro kit (Qiagen), reverse transcribed from mRNA to cDNA for subsequent real-time PCR analysis ...
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bioRxiv - Immunology 2024Quote: ... total RNA was extracted from T cells by using RNeasy mini kit (QIAGEN) and reverse-transcribed into cDNA with Superiorscript III reverse transcriptase (Enzynomics ...
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bioRxiv - Immunology 2024Quote: T cells were subjected to RNA extraction utilizing the RNeasy Micro Kit (QIAGEN). RNA from the renal cortex was isolated with the NucleoSpin Kit (Macharey-Nagel ...
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bioRxiv - Immunology 2021Quote: Between 104 and 3.104 murine and human T cells were sorted from lymph nodes and tumors in TCL buffer (Qiagen) with 1% of β-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from sorted CD4+ T cells using the RNeasy Mini kit (Qiagen). A total of 8.5 µL extracted RNA was subjected to one-step RT-PCR using the Superscript III one-step RT-PCR system (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: Real-time quantitative PCR (RT-qPCR) was done as previously described [Zonneveld 2021] using the Human T cell Tolerance & Anergy RT2 Profiler PCR array (Qiagen). The relative expression levels of each gene were normalized using 4 reference genes (B2M ...
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bioRxiv - Clinical Trials 2019Quote: Genomic DNA from 1-10 Million CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen). In some experiments ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from CD4+ T cells using the DNeasy blood and tissue kit (Qiagen), according to manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... The total RNA of sorted T cells was extracted with an RNeasy Micro kit (Qiagen) and reverse transcribed using reverse transcribed using SuperScript III Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... primary T cells and BJ-5at fibroblasts using the RNeasy Plus Mini Kit (Qiagen. #74136), reverse transcribed to first-stand cDNAs using iScript™ cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was extracted from sorted T cell populations with mini RNA-easy Kit (Qiagen). Equal amounts of total RNA from different groups were used for the assay ...
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bioRxiv - Immunology 2022Quote: ... DNA for library preparation was extracted from CD8+ T-cells using FlexiGene DNA Kit (Qiagen). 150 ng aliquots of obtained DNA were used as input to prepare each out of four TRA libraries ...
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bioRxiv - Immunology 2022Quote: ... CD4+/CD8+ T cells and Tfh from indicated organs using RNeasy Mini Kit (Qiagen Inc.). cDNA synthesis was performed using Superscript III First Strand Synthesis System (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... isolate DNA of CAR-T cell pellets with DNEasy Blood & Tissue Kit (Qiagen catalogue # 69506), perform PCR amplification with appropriate forward and reverse primers to TSC2 S1364A Δ insertion and then submit PRC products for sequencing (Genewiz ...
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bioRxiv - Immunology 2023Quote: T-cells or homogenized colon were suspended in RLT buffer (RNAeasy kit, Qiagen, Valencia, CA), RNA was extracted (RNAeasy ...
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bioRxiv - Immunology 2023Quote: ... RNA was extracted from CAR T cells using a Qiagen RNeasy Mini Kit (Qiagen; #74104) and RNA was quantified by NanoDrop Spectrophotometer (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 5×106 human PBMCs from Cohort II using AllPrep RNEasy kits (Qiagen) and the small RNA-containing column flow-through collected ...
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bioRxiv - Immunology 2021Quote: ... viable in vitro differentiated CD4+ T cells were sorted into 96-well plates containing 5 μl TCL Buffer (QIAGEN) with 1% 2-mercaptoethanol ...
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bioRxiv - Immunology 2020Quote: ... human sputum cells or mouse lung tissue using an RNeasy kit (Qiagen). 2μg was utilised for cDNA synthesis using the Omniscript RT kit (Qiagen) ...
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bioRxiv - Immunology 2020Quote: RNA from CD27/CD45RA defined CD4+ T cell subsets was isolated using the RNeasy kit (Qiagen) according the manufactures instructions ...
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bioRxiv - Cell Biology 2021Quote: RNA extraction from murine CD4+ T cells was performed using the RNeasy Plus Mini Kit (Qiagen) and cDNA was generated using the QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from FACS sorted target memory CD4+ T cells with RNeasy Micro Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted from FACS sorted target memory CD4+ T cells with RNeasy Micro Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... DNA was extracted from the T cells using the DNeasy Blood and Tissue kit (Qiagen, 69504) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was isolated from FACS-sorted T cell populations using genomic DNA isolation kits (Qiagen). Purified genomic DNA was subjected to bisulfite treatment with the Qiagen EpiTect bisulfite kit ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from the T cells using the RNeasy Mini RNA Isolation Kit (Qiagen). TCR α- and β-chain genes were reverse transcribed and amplified from the RNA using the SMARTer RACE Kit (Clontech Laboratories ...