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Citations for Qiagen :
1 - 50 of 10000+ citations for Human Low affinity immunoglobulin gamma Fc region receptor II c FCGR2C ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... flanking region PCR products were gel purified (QIAEX II Kit; Qiagen), digested with XbaI (NEB) ...
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bioRxiv - Immunology 2020Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cβ primer ...
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bioRxiv - Immunology 2024Quote: ... The cDNA library was amplified using a second set of multiple internally nested V-region and C-region primers with HotStarTaq DNA polymerase kit (Qiagen). The final PCR reaction was performed on an aliquot of the second reaction using a primer containing common base sequence and a third internally nested Cý primer ...
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bioRxiv - Biophysics 2021Quote: ... and Fc-tag ACE2 protein was purified using a protein affinity A column (Qiagen). Proteins were further purified by gel filtration (Superdex™ 200 Increase 10/30GL ...
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bioRxiv - Microbiology 2024Quote: ... Flag-SHOC2 was cleaved from 6X-His-MBP by incubating 37 mg purified protein with 0.65 mg TEV protease at 4°C overnight followed by affinity purification using Nickel affinity resin (Qiagen) where cleaved Flag-SHOC2 was collected in the flow-through.
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bioRxiv - Molecular Biology 2020Quote: ... were separated in 1.2% (w/v) low melting agarose gels and the ∼0.6 kb band was purified (QIAEX II Gel Extraction kit, Qiagen). This fragment was used as a template in a second PCR reaction with oligonucleotides VHH-Sfi2 (5′-GTC CTC GCA ACT GCG GCC CAG CCGGCC ATG GCT CAG GTG CAG CTG GTG GA-3’ ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 20,000 rpm for 1 hour at 4°C and the soluble fraction was affinity purified by gravity column with Ni-NTA affinity resin (QIAGEN). The protein was eluted by 50 mM Tris pH 8 ...
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bioRxiv - Immunology 2022Quote: ... For RT2 Profiler PCR Array Human Interferons and Receptors (Cat# PAHS064ZC-12, Qiagen, Germantown, MD), RNA extraction (RNeasy Pls Mini kit ...
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bioRxiv - Cancer Biology 2022Quote: c-miR isolations from blood serum were carried out using affinity column-based miRNeasy Serum/Plasma Advanced Kit (217204, Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 5×106 human PBMCs from Cohort II using AllPrep RNEasy kits (Qiagen) and the small RNA-containing column flow-through collected ...
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bioRxiv - Neuroscience 2022Quote: Brain tissue from the four specified regions was homogenized using the TissueLyser II (QIAGEN, Germantown, MD) in 10 vol ...
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bioRxiv - Biochemistry 2019Quote: ... at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen) followed by TEV protease treatment to remove the tag ...
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bioRxiv - Immunology 2020Quote: ... cDNA was added to the Human Toll-like receptor signaling pathway RT2 Profiler PCR array (Qiagen) and run on an iCycler MyiQ (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... The RT2 Profiler “Inflammatory cytokines and receptors” and “Human innate and adaptive response” arrays PCR Arrays (Qiagen) were performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and further disrupted by TissueLyser II (QIAGEN, C.0659). 100 mg ground sample was first resuspended in 1 mL lysis buffer (1×PBS ...
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bioRxiv - Neuroscience 2021Quote: A region-specific polymerase chain reaction (PCR) was performed for the S-COMT promoter region using the PyroMark PCR Kit (Qiagen, Hilden, GER) according to manufacturer’s instructions with previously published primers (F ...
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bioRxiv - Cancer Biology 2022Quote: ... and further purified with affinity column (RNeasy Mini Kit, Qiagen) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... HsKinesin-II was purified using Ni column and MBP column successively (Ni-NTA agarose affinity resin, QIAGEN; Amylose resin ...
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bioRxiv - Genetics 2022Quote: ... mouse brain and brain region tissues using the miRNeasy kit (QIAGEN, # 217004) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... miScript II RT Kit (Qiagen) was used to synthesize cDNA ...
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bioRxiv - Genetics 2021Quote: RNA was extracted from half of a 4 mm biopsy punch for each region of the brain using the Qiagen TissueLyser II for tissue disruption with 5mm stainless steel beads (Qiagen). The RNeasy Plus kit (Qiagen ...
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bioRxiv - Genomics 2022Quote: ... Age-associated regions were amplified using the PyroMark PCR Kit (Qiagen, Hilden, Germany) with manufactures instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... or miScript II RT Kit (Qiagen). Noteworthy ...
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bioRxiv - Developmental Biology 2021Quote: ... or miScript II RT kit (Qiagen). The qRT-PCR was carried out using Light Cycler 480 SYBR Green I Master (Roche) ...
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bioRxiv - Bioengineering 2021Quote: The miScript II RT kit (Qiagen) was used according to manufacturer’s protocol for cDNA synthesis from 1 μg of RNA ...
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bioRxiv - Physiology 2022Quote: ... miScript II RT Kit (Qiagen, UK) was used and cDNA synthesis was performed according to manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: ... 1 µg of RNA isolated from human islets or 10 µL of exosomal RNA was reverse transcribed using the miRScript II kit (Qiagen, Germany), following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity chromatography was carried out at 4°C with a Ni-NTA sepharose column (Qiagen, Hilden, Germany). The column was pre-equilibrated with 50 mM NaH2PO4-buffer pH 7.6 ...
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bioRxiv - Molecular Biology 2021Quote: ... at 55°C for 8h followed by 20 min heat inactivation at 80°C and gel purified using the QIAEX II Gel Extraction Kit (Qiagen). The gene fragments were ligated into the backbone using T4 DNA ligase (NEB ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... RNA was isolated from both brain regions using the AllPrep RNA Micro Kit (Qiagen) and reverse transcribed to cDNA using the SuperScript III First-Strand Synthesis System for RT-PCR applications ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA of different brain regions was isolated using the RNeasy extraction kit (Qiagen), and cDNA was synthesized after Deoxyribonuclease I (Invitrogen)treatment by using EvoScript Universal cDNA Master(Roche) ...
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bioRxiv - Neuroscience 2023Quote: ... Hippocampi were placed in protein low-binding tubes mounted onto a Qiagen TissueLyser II (Qiagen cat. Nr. 85300). Lysates were span down at 20.000 rcf for 20 minutes at 4°C to remove debris ...
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bioRxiv - Cell Biology 2024Quote: ... a second transfection of a 0.6 μg plasmid encoding either the C-terminal GFP11 tagged M2tail(368-466) or M2 receptor was performed using the Effectene Transfection Reagent (Qiagen). After washing each well with 2 mL PBS ...
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bioRxiv - Biochemistry 2019Quote: ... genomic DNA was de-crosslinked in ChIP elution buffer containing 5 M NaCl at 65 °C overnight and purified with the Qiaex II kit (Qiagen, 20021) and eluted in water for PCR amplification ...
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bioRxiv - Neuroscience 2023Quote: ... at a frequency of 28/s @ 4°C (Tissue Lyser II, Qiagen). After this step the samples were adjusted to RT for 5 minutes and processed using the RNeasy Lipid Tissue Mini Kit (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... and purified in buffer C (20 mM Tris, 100 mM NaCl, pH 7.5) by nickel-affinity chromatography (Qiagen) followed by size-exclusion chromatography on a Superdex 200 column in buffer D (20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C for 1 h and purified by affinity chromatography using a 5 ml Ni-HP column (Qiagen). Flow through with pure AstaPo1 was collected and dialyzed overnight against the buffer containing 50 mM Tris pH 7.6 ...
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bioRxiv - Genetics 2019Quote: ... and purified with QIAEX II kit (Qiagen). PCR products from the first step were used as templates in the second PCR step ...
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bioRxiv - Cancer Biology 2022Quote: ... miScript II RT Kit (Qiagen, Hilden, Germany) was used to convert the RNA to cDNA via a thermocycler (Eppendorf ...
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bioRxiv - Biochemistry 2020Quote: ... low melting point agarose gel and the approximately 700 base pair band corresponding to the heavy chain only immunoglobulin was extracted and purified using QIAquick Gel Extraction Kit (Qiagen). A secondary amplification of the VHH domain was performed using modified primers (VHH-Esp-For ...
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bioRxiv - Genomics 2020Quote: ... The regions of interest were subsequently amplified by PCR using the PyroMark PCR kit (Qiagen; primers listed in Supplemental Table S9) ...
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bioRxiv - Neuroscience 2019Quote: Total RNA was extracted from distinct mouse brain regions using the RNeasy Mini Kit (QIAGEN). The QuantiTect Reverse Transcription Kit (QIAGEN ...
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bioRxiv - Genetics 2019Quote: ... genomic regions were PCR amplified using sequence specific primers with HotStarTaq Polymerase Kit (Qiagen # 203203). PCR primer sequences used to amplify the CASK_SS variant ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was extracted from brain regions with a Qiagen RNAeasy mini kit (Qiagen, Hilden, Germany). There were eight mice in each experimental group ...
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bioRxiv - Biophysics 2023Quote: ... QIAEX II Gel Extraction Kit was used (50 µL of QIAEX II beads, QIAGEN). The samples were washed twice with PE buffer and eluted twice by 125 µL of 10 mM Tris-HCI buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The affinity purification was performed using Ni-NTA affinity column (Qiagen, Venlo, Netherlands), and the purified CyaY protein was then placed in the dialysis buffer and treated by Ulp1 to remove the N-terminal hexahistidine-Sumo tag ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was extracted from the same tumor region as the single-cell analyses using the Qiagen AllPrep kit and matched normal blood using DNeasy kit (Qiagen). Briefly ...
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bioRxiv - Immunology 2023Quote: For protein production a pT350 plasmid encoding for the construct of interest with a BiP signal sequence in the N-terminal and a double strep-tag in the C-terminal region was co-transfected with a puromycin resistant plasmid (pCoPuro) (61) with effectene reagent (Qiagen, 301425) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... froze them at –80°C and pulverized them using a TissueLyser II (Qiagen). We extracted DNA from the samples with DNAEasy Plant Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... NsiI and XhoI and gel purified to remove regions of CTCF homology (Qiagen Gel Extraction kit). ∼900 bp homology arms to Usp9x were amplified from mouse genomic DNA using PrimeStar GXL polymerase (Takara ...