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Citations for Qiagen :
1 - 50 of 10000+ citations for Human H ACA Ribonucleoprotein Complex Subunit 1 GAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... for 1 h at 37 °C and purified using QIAquick PCR Purification Kit (Qiagen). The pLVX-Puro lentiviral expression vector (Clontech ...
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bioRxiv - Microbiology 2023Quote: ... and The Protein Complex Suite (Qiagen). One microliter of 4.6 mg/ml protein sample suspended in crystallization buffer (25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from RPE/choroid complex and primary RPE cells using a RNA extraction kit (RNeasy plus mini kit QIAGEN). The RNA concentration and integrity were determined with NanoDrop 1000 spectrophotometer ...
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bioRxiv - Neuroscience 2023Quote: ... for 24 h using an RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... along with the human NaV β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.X:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transiently co-transfected with NaV1.2 and the accessory β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.2:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tags ...
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bioRxiv - Biochemistry 2021Quote: ... against Gαs subunit and mouse poly-His antibody (cat. no. 34660, QIAGEN) against His tag ...
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bioRxiv - Biochemistry 2021Quote: ... against Gαs subunit and mouse penta-His antibody (cat. no. 34660, QIAGEN) against His tags ...
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bioRxiv - Molecular Biology 2019Quote: ... The complex was purified using Ni-NTA agarose (Qiagen) followed by cleavage of the tags with TEV and SUMO proteases ...
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bioRxiv - Genomics 2022Quote: ... The immunoprecipitated complex was treated with RNaseA (QIAGEN, 19101), and reverse-crosslinked overnight at 68°C ...
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bioRxiv - Biochemistry 2020Quote: ... The complex was purified by Ni-NTA Superflow (Qiagen) and chitin bead (NEB ...
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bioRxiv - Genetics 2021Quote: Multi-array ELISA was performed using the Multi-Analyte ELISArray Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... and 72 h after induction using RNeasy Plant Mini Kit (Qiagen). RNA-seq analysis was carried out by Hokkaido System Science Co. ...
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bioRxiv - Microbiology 2019Quote: ... and 66 h post-infection using QIAmp DNA micro kit (QIAGEN). Viral DNA forms of wild-type and mutant HIV-1 were amplified using real-time PCR (Butler et al. ...
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bioRxiv - Plant Biology 2022Quote: ... 6 or 24 h using an RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: RNA was isolated from 2 h and 10 h RPMI-grown and macrophage-internalized Cg cells using the RNeasy kit (Qiagen), followed by DNase I digestion ...
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bioRxiv - Evolutionary Biology 2024Quote: ... alphenor females that were homozygous for the h or H alleles using the QIAgen GenomicTip G-100 kit (QIAgen, USA). Extractions followed the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The eluant was treated with DNase I (1 U/μl) to digest non-specific DNA and the bound DNA was purified from the complex via PCR Qiagen Kit (Qiagen). The purified DNA was amplified with primer sets specific to the prrF1,F2 promoter (Table S2 ...
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bioRxiv - Biochemistry 2021Quote: ... the purified complex was applied to Ni-NTA agarose (Qiagen) and rotated 60’ at 4° C ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 µM A740003 or both BzATP and A740003 together (A740003 was pre incubated for 1 h before adding BzATP) 24 hours later RNA was extracted using the miRNeasy mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmids containing qPCR amplicon sequences were linearized by digestion with SmaI for 1 h and purified using the QIAquick PCR purification kit (Qiagen). Linearized plasmids were quantified using spectrophotometry ...
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bioRxiv - Microbiology 2022Quote: ... were inoculated in 20 mL tryptone soy broth and incubated at 37°C for 24 h before 1 mL was pelleted and DNA extracted using the DNeasy Blood & Tissue kit (Qiagen). Libraries for genome sequencing were prepared using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... (1 μl) for 18 h and the RNA was extracted by using the mirCury cell and plant isolation kit (QIAGEN). A final number of 108 treated cells were lysed using 500 μL of glass beads and the 600 μL of the Lysis buffer provided with the kit ...
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bioRxiv - Microbiology 2020Quote: ... The PCR fragment was then digested with Dpn I at 37 °C for 1 h and purified using the QIAquick PCR purification kit (Qiagen). To prepare the competent SW102 containing the pSMART BAC-T7-scv2-replicon ...
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bioRxiv - Genomics 2020Quote: ... Amplified DNA (2ul from a 1:20 dilution of a 16 h REPLI-g Mini Kit whole-genome amplification, Qiagen) from each sorted population was tested in a 96-well plate in duplicate according to the manufacturer’s protocol ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6int and CCR6high subsets of CD4+ T cells were used for isolating RNA without and with prior activation with 20 ng/ml PMA and 1 mM ionomycin for 3 h at 37°C under 5% CO2 using RNeasy Mini Kit (Qiagen) and gene expression levels were determined using Illumina Human WG6 Expression BeadChip Version 3 arrays (Illumina Inc. ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from untreated cells (24 h) and IFNγ-treated cells (6 or 24 h) were extracted with RNeasy plus mini kit (Qiagen, 74134) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h or 24 h) using RNeasy Kit (Qiagen, #74104) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from INS-1 and human islets 48 hours post transfection using RNeasy cleanup kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: The LRH-1-10CA-TIF2 complex was concentrated to 5 mg/mL and screened using the Classics screen (Qiagen) and a Phoenix Liquid Handler (Art Robbins Instruments ...
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bioRxiv - Microbiology 2020Quote: ... and 8 h post-infection) using the QIAamp DNA micro kit (QIAGEN). As a parallel control ...
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bioRxiv - Zoology 2020Quote: ... SDS and 100 µg/ml proteinase K) and incubated at 56°C for 1 h before extraction using a QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s directions ...
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bioRxiv - Zoology 2023Quote: ... The mixtures were incubated at 56 °C for 1 h before DNA was extracted using an QIAamp DNA Mini Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from OSU-CLL cells (1.5e6 cells/mL) treated with DMSO or SpiD3 (1, 2 μM; 4 h) using the miRNeasy Mini Kit (Qiagen; Hilden, Germany) per manufacturer instructions and processed using the Universal Plus mRNA-Seq with NuQuant kit (Tecan ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from both initial mixtures (0 h incubation) and end point co-cultures (72 h incubation) using the DNeasy Power Biofilm extraction kit (DNeasy PowerBiofilm, Qiagen, Mississauga, ON) as described previously [5] ...
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bioRxiv - Immunology 2023Quote: ... RNA from human PBMCs was isolated using RNeasy Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Initial screens were performed using Nucleix and Protein Complex suites (Qiagen) in a sitting-drop setup ...
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bioRxiv - Microbiology 2019Quote: ... Initial crystallization conditions were obtained with the Protein Complex Suite (Qiagen) screened by sitting drop-vapor diffusion at 18 °C using a Mosquito robot and were optimized in hanging drops by mixing 1 μL of the sample with 1 μL of the precipitant ...
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bioRxiv - Biochemistry 2020Quote: Initial crystals of Nup133NTD-VHH-SAN5 were obtained at 18°C in 1 day as part of the Protein Complex suite (Qiagen) in a 96-well sitting drop tray with a reservoir containing 15% PEG MME 2,000 ...
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bioRxiv - Bioengineering 2023Quote: ... 48 and 72 h after transfection using an RNeasy Mini Plus kit (QIAGEN). Synthesis of first-strand cDNA from total RNA was performed with HiScript® III All-in-one RT SuperMix Perfect for qPCR (Vazyme) ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells treated for 48 h with 1 mM aspirin and 4 μM regorafenib using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen Cat# 80004) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 1.2% and lysozyme 20mg/ml) and incubated at 37°C for 1 h and DNA was extracted with the DNeasy blood&Tissue kit (Qiagen, Courtaboeuf, France) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... at 37 °C for 1 h and recovered by using RNeasy MinElute Cleanup kit as recommended by the manufacturer (Qiagen, Hilden, Germany). RNA yields were estimated by employing a Qubit® Fluorometer as recommended by the manufacturer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... human THP-1 macrophages (MACs) and acutely isolated mouse microglia was extracted using the RNeasy Plus Mini kit (Qiagen, 74136) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... astrocytes and human brain tissue using the RNeasy Mini kit (Qiagen) and reverse transcribed using the High-Capacity RNA-to-cDNA kit (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... the complex was purified by nickel affinity chromatography (Ni-NTA resin, Qiagen) and eluted with elution buffer (40mM Tris pH 8 ...
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bioRxiv - Biochemistry 2022Quote: PaPcc1-PaPcc2 complex was purified by affinity chromatography on NiNTA column (Qiagen) at room temperature followed by size exclusion chromatography as described above except that the lysis buffer contained 300 mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: RNA from human microglia differentiated in vitro and human microglia extracted from human-mouse chimeras was extracted using the RNeasy Plus Micro Kit (Qiagen). The RNA from 3 independent in vitro microglia differentiations per cell line were pooled at equal weight to obtain six samples ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted 48 h post-transfection using the RNeasy Mini Kit (Qiagen) following the manufacturer’s instructions ...